Hello Vidya,

It seems that your iProphet file might contain incorrect/incomplete
file paths for the source mzXML files.  What steps did you follow to
generate this file?  Feel free to email it to me so I can further
diagnose.

As you found out, you can always just open the PeptideProphet results
file in Pep3D, or the mzXML/mzML raw data.

Cheers,
--Luis


On Wed, Oct 24, 2012 at 5:02 PM, Vidya Venkatraman
<vidya.venk...@gmail.com> wrote:
> Hi,
>
> I have received searched Xtandem data (from 1 raw file) which was also run
> through peptide and protein prophet. I am trying to open the iprophet
> pep.xml file through TPP in the pep3d viewer. But when i click on generate
> 3d image, it errors saying could not open input file. Also, you can see how
> the mzxml does not appear such that it can be selected.
>
> I just wanted to check on how one can import iprophet pep.xml results onto
> pep3d viewer if it was searched and run through peptide prophet elsewhere. I
> do have the fasta, mzxml, pep.xml, ipep.xml, prot.xml under
> C:\Inetpub\wwwroot\ISB\data\data.
>
> Could it be the naming convention eg. the files need to have the same name?
> What files does it look for or what files do i need to have for this to
> work?
>
> Lastly, when i took the pep.xml file and ran it through peptide prophet on
> the TPP gui (instead of starting with the ipep file), it does seem to work
> and generate the pep3d image.
>
> I dont use TPP that often, so this could be something obvious. But i
> appreciate any guidance on this matter.
>
> Thanks in advance,
> Vidya
>
>
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