Hi, Henry,
I have the same problem. I use the the Comet to do the search, and when I 
use Libra function in peptide analysis module, I got this message: 

 Error ms2 filter line: 

I don't really know what causes this, anyone in TPP team know how to solve this?


SunSun



On Sunday, March 29, 2015 at 8:10:34 AM UTC+8, Henry Rodríguez wrote:
>
> Hi, 
>
> I am performing a quantification of my data with Libra software. However, 
> I always obtain a error. My original spectra are in *MGF format*, so I 
> convert them to mzML with MSConvert software of Proteowizard with different 
> filters, but this does not functioned. Also, I have tried use the 
> ScanRanker software which filter for quality the spectra (
> http://fenchurch.mc.vanderbilt.edu/bumbershoot/scanranker/index.html), 
> but this also does not functioned. The error that I have obtained is the 
> following. Thanks for your help with this error,
>
> Henry.
>
>
>
> Analyzing interact_rawdata280315.pep.xml ...
> Parsing search results 
> "c:/Inetpub/wwwroot/ISB/data/Rawdata_Peakpicking2/PAIV141024_1 (X! Tandem 
> (k-score))"...
>   => Found 34 hits. (0 decoys, 0 excluded)
>   => Total so far: 34 hits. (0 decoys, 0 excluded)
> Parsing search results 
> "c:/Inetpub/wwwroot/ISB/data/Rawdata_Peakpicking2/PAIV141024_2 (X! Tandem 
> (k-score))"...
>   => Found 68 hits. (0 decoys, 0 excluded)
>   => Total so far: 102 hits. (0 decoys, 0 excluded)
> El sistema no puede encontrar la ruta especificada.
> command completed in 1 sec 
>
> running: "C:/Inetpub/tpp-bin/LibraPeptideParser 
> "interact_rawdata280315.pep.xml" -ccondition.xml"
>  Error ms2 filter line: 
>
>
> command "C:/Inetpub/tpp-bin/LibraPeptideParser 
> "interact_rawdata280315.pep.xml" -ccondition.xml" failed: Operation not 
> permitted
>
> command "C:/Inetpub/tpp-bin/LibraPeptideParser 
> "interact_rawdata280315.pep.xml" -ccondition.xml" exited with non-zero exit 
> code: 1
> QUIT - the job is incomplete
>
>

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