Dear Jimmy,

I have a similar problem. I have replaced the parameter file to an older 
version and tried updated Comet version with the appropriate parameter file 
but I still get the error /190417_CytHumSt_1_Pep3D_Spectrum.pep.xml (*file 
not found:* not created or deleted?).
TPP no longer complains about the parameter file though and looks like it 
is going through the search but then seems to abruptly terminate the 
process:

Comet version "2019.01 rev. 5"

 Search start:  07/29/2020, 12:08:28 PM
 - Input file: 190417_CytHumSt_1_Pep3D_Spectrum.mzML
   - Load spectra: 1458
     - Search progress:   4%
     - Post analysis:  done
 Search end:    07/29/2020, 12:08:30 PM, 0m:2s

Is there something else I could try?

Thank you,

Kate Mostovenko

On Monday, 13 April 2020 14:31:47 UTC-4, Jimmy Eng wrote:
>
> Javan,
>
> As the message states, the Comet binary is for version 2018.01 yet you are 
> attempting to use a comet.params file for newer Comet version 2019.01.  You 
> have 2 options.  
>
>    1. The first is to get rid of the comet.params you're using and grab 
>    one for version 2018.01.   At the top of this page 
>    <http://comet-ms.sourceforge.net/parameters/parameters_201801/>, you 
>    can download example comet.params files for version 2018.01.  Rename them 
>    to just "comet.params", modify parameters as needed, and try the search 
>    again.
>    2. Your second option is to stick with your 2019.01 parameters file 
>    and update the Comet executable.  I would personally go this route.  To do 
>    that, go here and download the comet_2019015.zip file 
>    <https://sourceforge.net/projects/comet-ms/files/>.  Grab the 
>    comet.2019015.win64.exe binary within that zip archive, rename it to 
>    comet.exe and place it in c:\TPP\bin\.  You will be replacing the existing 
>    2018.01.4 version of comet.exe in that directory (which you may consider 
>    backing up before replacing).  Try the search again after updating the 
>    binary.
>
> Good luck.
>
> On Mon, Apr 13, 2020 at 11:18 AM Javan Okendo <javan...@gmail.com 
> <javascript:>> wrote:
>
>> Hi,
>>
>>  How are you attempting to running Comet? 
>> I am running this data using the TPP program. 
>>
>> The program gives this message
>>
>> Comet version 2018.01 rev. 4
>>
>>  The comet.params file is from version 2019.01
>>  Please update your comet.params file.  You can generate
>>  a new parameters file using "comet -p"
>>
>>
>>
>>
>> On Mon, Apr 13, 2020 at 7:40 PM Jimmy Eng <jke...@gmail.com <javascript:>> 
>> wrote:
>>
>>> Javan,
>>>
>>> This implies the Comet search has not run.  How are you attempting to 
>>> running Comet?  You mention searchGUI in your title ... are you using the 
>>> TPP to run Comet or CometOmic's SearchGUI?
>>>
>>> Jimmy
>>>
>>> On Mon, Apr 13, 2020 at 10:27 AM JAVAN OKENDO <javan...@gmail.com 
>>> <javascript:>> wrote:
>>>
>>>> I get the following error even though my mzML raw files are in the 
>>>> "Data" dir. Why is the program complaining about the .pepXML not found and 
>>>> yet am doing the comet search? I am stuck and I disparately need your 
>>>> assistance
>>>>
>>>>    - c:/Data/170909_CH_C1_T006_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T007_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T009_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T010_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T011_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T013_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T014_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T015_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T025_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T026_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T027_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T037_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T042_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T049_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T051_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T052_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T053_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T054_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T061_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T062_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T064_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T069_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T073_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T075_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T077_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T080_FT.pep.xml (*file not found:* not 
>>>>    created or deleted?)
>>>>    - c:/Data/170909_CH_C1_T084_FT_R2.pep.xml (*file not found:* not 
>>>>    created or deleted?
>>>>
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