Dear Jimmy, I have a similar problem. I have replaced the parameter file to an older version and tried updated Comet version with the appropriate parameter file but I still get the error /190417_CytHumSt_1_Pep3D_Spectrum.pep.xml (*file not found:* not created or deleted?). TPP no longer complains about the parameter file though and looks like it is going through the search but then seems to abruptly terminate the process:
Comet version "2019.01 rev. 5" Search start: 07/29/2020, 12:08:28 PM - Input file: 190417_CytHumSt_1_Pep3D_Spectrum.mzML - Load spectra: 1458 - Search progress: 4% - Post analysis: done Search end: 07/29/2020, 12:08:30 PM, 0m:2s Is there something else I could try? Thank you, Kate Mostovenko On Monday, 13 April 2020 14:31:47 UTC-4, Jimmy Eng wrote: > > Javan, > > As the message states, the Comet binary is for version 2018.01 yet you are > attempting to use a comet.params file for newer Comet version 2019.01. You > have 2 options. > > 1. The first is to get rid of the comet.params you're using and grab > one for version 2018.01. At the top of this page > <http://comet-ms.sourceforge.net/parameters/parameters_201801/>, you > can download example comet.params files for version 2018.01. Rename them > to just "comet.params", modify parameters as needed, and try the search > again. > 2. Your second option is to stick with your 2019.01 parameters file > and update the Comet executable. I would personally go this route. To do > that, go here and download the comet_2019015.zip file > <https://sourceforge.net/projects/comet-ms/files/>. Grab the > comet.2019015.win64.exe binary within that zip archive, rename it to > comet.exe and place it in c:\TPP\bin\. You will be replacing the existing > 2018.01.4 version of comet.exe in that directory (which you may consider > backing up before replacing). Try the search again after updating the > binary. > > Good luck. > > On Mon, Apr 13, 2020 at 11:18 AM Javan Okendo <javan...@gmail.com > <javascript:>> wrote: > >> Hi, >> >> How are you attempting to running Comet? >> I am running this data using the TPP program. >> >> The program gives this message >> >> Comet version 2018.01 rev. 4 >> >> The comet.params file is from version 2019.01 >> Please update your comet.params file. You can generate >> a new parameters file using "comet -p" >> >> >> >> >> On Mon, Apr 13, 2020 at 7:40 PM Jimmy Eng <jke...@gmail.com <javascript:>> >> wrote: >> >>> Javan, >>> >>> This implies the Comet search has not run. How are you attempting to >>> running Comet? You mention searchGUI in your title ... are you using the >>> TPP to run Comet or CometOmic's SearchGUI? >>> >>> Jimmy >>> >>> On Mon, Apr 13, 2020 at 10:27 AM JAVAN OKENDO <javan...@gmail.com >>> <javascript:>> wrote: >>> >>>> I get the following error even though my mzML raw files are in the >>>> "Data" dir. Why is the program complaining about the .pepXML not found and >>>> yet am doing the comet search? I am stuck and I disparately need your >>>> assistance >>>> >>>> - c:/Data/170909_CH_C1_T006_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T007_FT_R2.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T009_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T010_FT_R2.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T011_FT_R2.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T013_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T014_FT_R2.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T015_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T025_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T026_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T027_FT_R2.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T037_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T042_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T049_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T051_FT_R2.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T052_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T053_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T054_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T061_FT_R2.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T062_FT_R2.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T064_FT_R2.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T069_FT_R2.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T073_FT_R2.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T075_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T077_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T080_FT.pep.xml (*file not found:* not >>>> created or deleted?) >>>> - c:/Data/170909_CH_C1_T084_FT_R2.pep.xml (*file not found:* not >>>> created or deleted? >>>> >>>> -- >>>> You received this message because you are subscribed to the Google >>>> Groups "spctools-discuss" group. >>>> To unsubscribe from this group and stop receiving emails from it, send >>>> an email to spctools...@googlegroups.com <javascript:>. >>>> To view this discussion on the web visit >>>> https://groups.google.com/d/msgid/spctools-discuss/aa0607da-e037-4a8c-a94f-4bbfb5eab0e8%40googlegroups.com >>>> >>>> <https://groups.google.com/d/msgid/spctools-discuss/aa0607da-e037-4a8c-a94f-4bbfb5eab0e8%40googlegroups.com?utm_medium=email&utm_source=footer> >>>> . >>>> >>> -- >>> You received this message because you are subscribed to the Google >>> Groups "spctools-discuss" group. >>> To unsubscribe from this group and stop receiving emails from it, send >>> an email to spctools...@googlegroups.com <javascript:>. >>> To view this discussion on the web visit >>> https://groups.google.com/d/msgid/spctools-discuss/CAJqD6EPk%3DzDb1yZPzAXMoDSCwH7mtNYWeMRiiy66R-A6j3Zx3Q%40mail.gmail.com >>> >>> <https://groups.google.com/d/msgid/spctools-discuss/CAJqD6EPk%3DzDb1yZPzAXMoDSCwH7mtNYWeMRiiy66R-A6j3Zx3Q%40mail.gmail.com?utm_medium=email&utm_source=footer> >>> . >>> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to spctools...@googlegroups.com <javascript:>. >> To view this discussion on the web visit >> https://groups.google.com/d/msgid/spctools-discuss/CAGdnHmveXWFvWgk7v6tsEE7j9UJLxuOphNtV%2BkVmy1f8HKxN8w%40mail.gmail.com >> >> <https://groups.google.com/d/msgid/spctools-discuss/CAGdnHmveXWFvWgk7v6tsEE7j9UJLxuOphNtV%2BkVmy1f8HKxN8w%40mail.gmail.com?utm_medium=email&utm_source=footer> >> . >> > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. 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