Hi Debojyoti, Yes, I would say this is the most straightforward way of conducting this search. Note that you may need to make further adjustments to the variable mod string settings if, say, a given peptide must have *all* of its Cysteines in one state (+50) or the other (+90). The default is to allow for mixed Cysteines in the peptide, e.g. PEPC[50]TIDEC[90]R. Hope this helps, --Luis
On Mon, Jul 31, 2023 at 11:04 AM Debojyoti Pal <debojyoti...@gmail.com> wrote: > Hello everyone > > I have a specific scenario where I want to search for peptide in which > cysteine must either have +50 or +90 mod. Basically: > 1) Cys can only have one of +50 or +90 > 2) Cys much have one of +50 or +90 (ie It cannot be unmodified) > > Only way I can achieve this is to put a static mod of +50 and variable mod > of +40 (40+50 = +90). Is there any better way to do this. > > Regards > Debojyoti > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to spctools-discuss+unsubscr...@googlegroups.com. > To view this discussion on the web visit > https://groups.google.com/d/msgid/spctools-discuss/e4fc0662-b880-4a69-863f-4261444ba547n%40googlegroups.com > <https://groups.google.com/d/msgid/spctools-discuss/e4fc0662-b880-4a69-863f-4261444ba547n%40googlegroups.com?utm_medium=email&utm_source=footer> > . > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CACyS9bpgorSzKSqYab8zAtpuVkmaPkg2-27iz%3Ds4PJe9Tqz2yA%40mail.gmail.com.