This should already be possible with, e.g. variable_mod02 = 40.000 C 0 3 -1 0 0 0.0 add_C_cysteine = 50.000
(Of course using whatever masses are appropriate.) Or does this not work? On Mon, Jul 31, 2023 at 9:31 PM Debojyoti Pal <debojyoti...@gmail.com> wrote: > Thanks Luis. I have asked this on the COMET group as well. They will > probably come up with an update or something to enable it. > On Tuesday, August 1, 2023 at 8:47:46 AM UTC+5:30 Luis Mendoza wrote: > >> Hi Debojyoti, >> Yes, I would say this is the most straightforward way of conducting this >> search. >> Note that you may need to make further adjustments to the variable mod >> string settings if, say, a given peptide must have *all* of its Cysteines >> in one state (+50) or the other (+90). The default is to allow for mixed >> Cysteines in the peptide, e.g. PEPC[50]TIDEC[90]R. >> Hope this helps, >> --Luis >> >> >> On Mon, Jul 31, 2023 at 11:04 AM Debojyoti Pal <debojy...@gmail.com> >> wrote: >> >>> Hello everyone >>> >>> I have a specific scenario where I want to search for peptide in which >>> cysteine must either have +50 or +90 mod. Basically: >>> 1) Cys can only have one of +50 or +90 >>> 2) Cys much have one of +50 or +90 (ie It cannot be unmodified) >>> >>> Only way I can achieve this is to put a static mod of +50 and variable >>> mod of +40 (40+50 = +90). Is there any better way to do this. >>> >>> Regards >>> Debojyoti >>> >>> -- >>> You received this message because you are subscribed to the Google >>> Groups "spctools-discuss" group. >>> To unsubscribe from this group and stop receiving emails from it, send >>> an email to spctools-discu...@googlegroups.com. >>> To view this discussion on the web visit >>> https://groups.google.com/d/msgid/spctools-discuss/e4fc0662-b880-4a69-863f-4261444ba547n%40googlegroups.com >>> <https://groups.google.com/d/msgid/spctools-discuss/e4fc0662-b880-4a69-863f-4261444ba547n%40googlegroups.com?utm_medium=email&utm_source=footer> >>> . >>> >> -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to spctools-discuss+unsubscr...@googlegroups.com. > To view this discussion on the web visit > https://groups.google.com/d/msgid/spctools-discuss/b4cd975a-1881-49a0-8a04-684c92bb5ae4n%40googlegroups.com > <https://groups.google.com/d/msgid/spctools-discuss/b4cd975a-1881-49a0-8a04-684c92bb5ae4n%40googlegroups.com?utm_medium=email&utm_source=footer> > . > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CACyS9bq1fOfCp6bc9UrdCjEvTcQeSxDmz%3D%3DmdGeBRVULqZ%3Dh8A%40mail.gmail.com.