I meant that they would come up with something to get it done with only variable modifications. By updating the code. Not by the static + variable workaround we are doing.
Thanks Debojyoti On Tue, Aug 1, 2023 at 10:14 AM 'Luis Mendoza' via spctools-discuss < spctools-discuss@googlegroups.com> wrote: > This should already be possible with, e.g. > > variable_mod02 = 40.000 C 0 3 -1 0 0 0.0 > add_C_cysteine = 50.000 > > (Of course using whatever masses are appropriate.) > > Or does this not work? > > > On Mon, Jul 31, 2023 at 9:31 PM Debojyoti Pal <debojyoti...@gmail.com> > wrote: > >> Thanks Luis. I have asked this on the COMET group as well. They will >> probably come up with an update or something to enable it. >> On Tuesday, August 1, 2023 at 8:47:46 AM UTC+5:30 Luis Mendoza wrote: >> >>> Hi Debojyoti, >>> Yes, I would say this is the most straightforward way of conducting this >>> search. >>> Note that you may need to make further adjustments to the variable mod >>> string settings if, say, a given peptide must have *all* of its Cysteines >>> in one state (+50) or the other (+90). The default is to allow for mixed >>> Cysteines in the peptide, e.g. PEPC[50]TIDEC[90]R. >>> Hope this helps, >>> --Luis >>> >>> >>> On Mon, Jul 31, 2023 at 11:04 AM Debojyoti Pal <debojy...@gmail.com> >>> wrote: >>> >>>> Hello everyone >>>> >>>> I have a specific scenario where I want to search for peptide in which >>>> cysteine must either have +50 or +90 mod. Basically: >>>> 1) Cys can only have one of +50 or +90 >>>> 2) Cys much have one of +50 or +90 (ie It cannot be unmodified) >>>> >>>> Only way I can achieve this is to put a static mod of +50 and variable >>>> mod of +40 (40+50 = +90). Is there any better way to do this. >>>> >>>> Regards >>>> Debojyoti >>>> >>>> -- >>>> You received this message because you are subscribed to the Google >>>> Groups "spctools-discuss" group. >>>> To unsubscribe from this group and stop receiving emails from it, send >>>> an email to spctools-discu...@googlegroups.com. >>>> To view this discussion on the web visit >>>> https://groups.google.com/d/msgid/spctools-discuss/e4fc0662-b880-4a69-863f-4261444ba547n%40googlegroups.com >>>> <https://groups.google.com/d/msgid/spctools-discuss/e4fc0662-b880-4a69-863f-4261444ba547n%40googlegroups.com?utm_medium=email&utm_source=footer> >>>> . >>>> >>> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to spctools-discuss+unsubscr...@googlegroups.com. >> To view this discussion on the web visit >> https://groups.google.com/d/msgid/spctools-discuss/b4cd975a-1881-49a0-8a04-684c92bb5ae4n%40googlegroups.com >> <https://groups.google.com/d/msgid/spctools-discuss/b4cd975a-1881-49a0-8a04-684c92bb5ae4n%40googlegroups.com?utm_medium=email&utm_source=footer> >> . >> > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to spctools-discuss+unsubscr...@googlegroups.com. > To view this discussion on the web visit > https://groups.google.com/d/msgid/spctools-discuss/CACyS9bq1fOfCp6bc9UrdCjEvTcQeSxDmz%3D%3DmdGeBRVULqZ%3Dh8A%40mail.gmail.com > <https://groups.google.com/d/msgid/spctools-discuss/CACyS9bq1fOfCp6bc9UrdCjEvTcQeSxDmz%3D%3DmdGeBRVULqZ%3Dh8A%40mail.gmail.com?utm_medium=email&utm_source=footer> > . > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CAFydq4bHfjyYmNYzOpyZ5%3DHqw2DYq9K-GgCeLkvrdmNe-Tj0Sw%40mail.gmail.com.
