Aleks said: >I've looked at the pictures and it seems to be a lipase catalyzed >reaction, hence the totally pure-looking glycerine. The murkiness of >the mixture is probably due to a water solution of the enzyme, the >water is then removed with the glycerine (lipase is active only in a >water solution, I'm told). It doesn't hurt the process, because ther >is no lye around and it doesn't have to be washed, at least not hard. >Remaining methanol could be left to evaporate, ant the settled ester >used immediately. The question is, is it as cheap as standard >processes? How do you obtain stabilized lipase at a fair price? >Cheers, Aleks >P.s. : it looks very elegant though, if I can get some lipase I'll >surely try it.
A small quantity of porcine lipase can be purchased from Worthington <http://www.worthington-biochem/com/priceList/L/Lipase.html> for US$21. There is also some good info at <http://www.worthington-biochem/manual/L/PL.html>. Many references are provided, which I have not yet read. Do you know for certain that methyl or ethyl esters would be created by adding alcohol to the lipase/oil emulsion? Perhaps alcohol would denature the enzyme and shut down the reaction? -- ...Warren Rekow ------------------------ Yahoo! Groups Sponsor ---------------------~-~> Make good on the promise you made at graduation to keep in touch. Classmates.com has over 14 million registered high school alumni--chances are you'll find your friends! http://us.click.yahoo.com/03IJGA/DMUCAA/4ihDAA/FZTVlB/TM ---------------------------------------------------------------------_-> Biofuel at Journey to Forever: http://journeytoforever.org/biofuel.html To unsubscribe, send an email to: [EMAIL PROTECTED] Your use of Yahoo! Groups is subject to http://docs.yahoo.com/info/terms/