Aleks replied: > > A small quantity of porcine lipase can be purchased from >Worthington >> <http://www.worthington-biochem/com/priceList/L/Lipase.html> for >> US$21. There is also some good info at >> <http://www.worthington-biochem/manual/L/PL.html>. Many references >> are provided, which I have not yet read. Do you know for certain >that >> methyl or ethyl esters would be created by adding alcohol to the >> lipase/oil emulsion? >Yepp. It has been done before. But now there are methods to recover >cheaply the overpriced lipase, and this certainly is a way to look >at. No harsh chemicals, giant pH leaps, no washing... 'ts the world >of wonderdiesel. It can create bio in a watery envyronment (actually >it needs some water), creates methyl esters, ethyl esters, butyl >esters, iso-propyl esters (the latter are said to be extremely winter >friendly)....
OK, and isopropanol is not so toxic like methanol, and not so government regulated like ethanol. Sounds promising. Where have you seen lipase recovery methods described? >Perhaps alcohol would denature the enzyme and >> shut down the reaction? >Short chain alcohols can damage unstabilized enzymes. But if I can >get the lipase to sit on little pieces of ceramics? Yes, I too have seen references to coating enzyme on a solid substrate, but don't know how the process is done. Aleks, is a university located anywhere near you? BTW, where are you? There are three small universities within 50 miles of me. When there is time I will stop in at one of them to see what relevant citations can be found in the chem abstracts, etc. Here in Idaho the U of I would likely be an excellent resource, but their campus is a several-hour drive from my home. -- ...Warren Rekow Biofuel at Journey to Forever: http://journeytoforever.org/biofuel.html To unsubscribe, send an email to: [EMAIL PROTECTED] Your use of Yahoo! Groups is subject to http://docs.yahoo.com/info/terms/
