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Dear Vince,
Since you crystallized in PEG 8000, try adding low molecular weight PEGs
(e.g. PEG 400 or PEG 600) to get a suitable cryo buffer. One good
reference is Garman and Schneider in J. Appl. Cryst. (1997), volume 30,
pages 211-237 (should be open access on the web).
If that fails, you could also try ethylene glycol, sucrose (or other
sugars), or MPD...
Good luck!
Lauren
Vincenzo Carbone wrote:
Hi guys
Sorry for the non ccp4 based question but i was wondering if anyone
has any tips on producing a suitable cryo buffer for crystals grown in
peg8000. My crystals come out quite quickly in 15 to 10% peg and can
be quite large. Ive tried to increase the peg concentration,
replacing the peg with glycerol or using a combination of peg8000 and
glycerol but each time the crystals fail to diffract when flash
frozen. Previous experience with these crystals have shown diffraction
of up to 2A when dry mounted.
thanks,
vince
--
Lauren Parker
MRC Laboratory of Molecular Biology
Structural Studies Division
Hills Road
Cambridge CB2 2QH
+44 1223 402 406
[EMAIL PROTECTED]
