Hi,
assuming that 1) you used the right MW cut-off and 2) the membrane is
intact, you probably "pelleted" your protein onto the membrane.
This often happens if the protein has a limited solubility in the buffer
you are using.
Remember, while concentrating your protein, you are actually "traveling"
through the solubility phase diagram.
- J. -
yanming Zhang wrote:
Hi all,
during concentrating my protein, using Amicon Ultra centrifugal filter
devices, 5000g, 4C, I lost large amount of my protein (75%). I heard
the same story from one of my colleagues too. It seems the membrane of
the Amicon tube ate my protein.
Why this happen and how to recover my protein?
Thank you very much.
Yan
--
Dr. Jeroen R. Mesters
Gruppenleiter Strukturelle Neurobiologie und Kristallogenese
Institut für Biochemie, Universität zu Lübeck
Zentrum für Medizinische Struktur- und Zellbiologie
Ratzeburger Allee 160, D-23538 Lübeck
Tel: +49-451-5004065, Fax: +49-451-5004068
Http://www.biochem.uni-luebeck.de
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