Check the speed--for the 15mL devices, ~3000 x g is the cutoff, I think. You
may be breaking the membranes, and your protein is simply in the bottom of the
concentrator.
JPK
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
*******************************************
----- Original Message -----
From: yanming Zhang
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, May 05, 2009 11:06 AM
Subject: [ccp4bb] Lost my protein
Hi all,
during concentrating my protein, using Amicon Ultra centrifugal filter
devices, 5000g, 4C, I lost large amount of my protein (75%). I heard the same
story from one of my colleagues too. It seems the membrane of the Amicon tube
ate my protein.
Why this happen and how to recover my protein?
Thank you very much.
Yan
