Hi Yanming, you can try adding 1 M of urea, 1% detergent (OG,DM) that often helps to keep proteins in solution.
Best wishes Kornelius On Tue, 5 May 2009 09:06:09 -0700 yanming Zhang <shanma...@yahoo.com> wrote: > Hi all, > > during concentrating my protein, using Amicon Ultra centrifugal filter > devices, 5000g, 4C, I lost large amount of my protein (75%). I heard the same > story from one of my colleagues too. It seems the membrane of the Amicon tube > ate my protein. > Why this happen and how to recover my protein? > > Thank you very much. > > Yan > > > > ---------------------------------------------- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany kornelius.z...@tuebingen.mpg.de Tel -49 7071 601 323 Fax -49 7071 601 349
