Hey Len, I had this problem, too. As you know, my favorite first try is always fomblin (no need to mix anything). I had quite a bit success in stubborn cases to inject about 4uL fomblin through the tape on top of the drop and then looping crystals through the oil layer. You can wick the mother liquor off and try them right away or continue manipulation under oil
Cheers, Jens On Wed, 2011-10-26 at 11:46 -0500, Leonard Thomas wrote: > Hi All, > > I have run into a very sensitive crystals system when it comes to cryo > protecting them. I have run through the usual suspects and trays are > going to be setup with a cryo protectant as part of crystallization > cocktail. The one problem that seems to be occurring is that the > crystals crack as soon as they are transfered out of the original > drop. I am running out of ideas and really would love some new ones. > > Thanks in advance. > > Len > > Leonard Thomas Ph.D. > Macromolecular Crystallography Laboratory Manager > University of Oklahoma > Department of Chemistry and Biochemistry > Stephenson Life Sciences Research Center > 101 Stephenson Parkway > Norman, OK 73019-5251 > > lmtho...@ou.edu > http://barlywine.chem.ou.edu > Office: (405)325-1126 > Lab: (405)325-7571
