Another possibility (other than those already mentioned) is to try
freezing without a cryoprotectant, by fishing the crystals out onto a
mesh and removing all the mother liquor.
The following paper has some details:
"Direct cryocooling of naked crystals: are cryoprotection agents
always necessary?"
Erika Pellegrini, Dario Pianoa, and Matthew W. Bowlera
Acta Cryst. (2011). D67, 902906
--
Andrew Purkiss
X-ray Laboratory Manager
Cancer Research UK
London Research Institute.
Quoting Leonard Thomas <lmtho...@ou.edu>:
Hi All,
I have run into a very sensitive crystals system when it comes to
cryo protecting them. I have run through the usual suspects and
trays are going to be setup with a cryo protectant as part of
crystallization cocktail. The one problem that seems to be
occurring is that the crystals crack as soon as they are transfered
out of the original drop. I am running out of ideas and really
would love some new ones.
Thanks in advance.
Len
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
Stephenson Life Sciences Research Center
101 Stephenson Parkway
Norman, OK 73019-5251
lmtho...@ou.edu
http://barlywine.chem.ou.edu
Office: (405)325-1126
Lab: (405)325-7571
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