One more thing you could try: high pressure cryo-cooling. Se any of a
number of paperas by Chae Un Kim; e.g.
http://www.ncbi.nlm.nih.gov/pubmed/17452791
Acta Crystallogr D Biol Crystallogr.
<http://www.ncbi.nlm.nih.gov/pubmed/17452791#> 2007 May;63(Pt 5):653-9.
Epub 2007 Apr 21.
On 10/26/11 12:46, Leonard Thomas wrote:
Hi All,
I have run into a very sensitive crystals system when it comes to cryo
protecting them. I have run through the usual suspects and trays are
going to be setup with a cryo protectant as part of crystallization
cocktail. The one problem that seems to be occurring is that the
crystals crack as soon as they are transfered out of the original
drop. I am running out of ideas and really would love some new ones.
Thanks in advance.
Len
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
Stephenson Life Sciences Research Center
101 Stephenson Parkway
Norman, OK 73019-5251
lmtho...@ou.edu
http://barlywine.chem.ou.edu
Office: (405)325-1126
Lab: (405)325-7571
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All Things Serve the Beam
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David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schul...@cornell.edu
