I remembered that people had crystallize a series of streptavidin-2-iminobiotin structures at a low pH. If it might help, check the following PDBIDs:
2RTD 2RTE 2RTI 2RTK 2RTL > Hi everyone > > I have a protein that is extraordinarily stable at PH=3.0 or even 2.0. > > I want to crystallize it in the low PH and compare the differences > between the crystals in regular PH and low PH. > > I was wondering how people set up the boxes in low PH, as usual buffers > are mostly less acidic. > > Regards > > Sam >
