Dow Corning Heavy Vacuum Grease, a 10 ml syringe and a shorter cut 200 µl tip attached to the syringe.
Jürgen On Nov 18, 2011, at 12:55 PM, Dima Klenchin wrote: Hello, I wonder what everyone is using for sealing hanging drop slides on VDX plates? For the most part, we paraffin oil but I am unhappy with it because it is too think and too frequently there is break in the oil and the drop dries too much. I find vacuum grease to be not terribly practical because it takes too much time - particularly when the well needs to be opened (seeding, modifying well content, etc). In ideal world I would like to find a much thicker oil that 1) contains as little volatiles as paraffin oil, and 2) allows no more water vapor diffusion through it than paraffin oil. Hampton suggests Cargille immersion oils for this purpose but the MSDS for these oils states that they have slight odor, so I am a bit concerned with unknown volatiles getting into crystallization drop. So, what do you like to use? Thanks much, - Dima ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://web.mac.com/bosch_lab/
