The is probably the cleanest and fastest way of greasing plates.
Take a 15ml Falcon tube and unscrew the cap. You will notice that the cap is exactly the width as the well of the VDX plate. Using a fairly wide bored metal syringe needle, pierce 8 holes into the cap from the outside, along the top edge of the cap. Fill the Falcon tube with Vaseline and screw the cap back onto the tube. Loosen the cap about 2 to 2.5 turns, just before it comes of the tube. Stick the tube in a heating block, wait for the Vaseline to melt. When you now invert the tube with the liquid Vaseline, it should not come running out, but if you press the top of the cap against the well of the VDX plate, you will see that a small amount of Vaseline is let out through the small holes. If you lift the tube off the well, air will then go into the tube and you can press the cap against the next well. Continue until you're. Should take no more than a few seconds to do the whole plate.
Cheers, Chris Browning -- Dr. Christopher Browning Post-Doctor to Prof. Petr Leiman EPFL BSP-416 1015 Lausanne Tel: 0041 (0) 02 16 93 04 40
