Vaseline (pure, with no additives) from CVS/Walgreens in a 10 ml syringe
and a pipette tip (wide bore) also works very well.
On 11/18/11 12:58 PM, Bosch, Juergen wrote:
Dow Corning Heavy Vacuum Grease, a 10 ml syringe and a shorter cut 200
µl tip attached to the syringe.
Jürgen
On Nov 18, 2011, at 12:55 PM, Dima Klenchin wrote:
Hello,
I wonder what everyone is using for sealing hanging drop slides on VDX
plates? For the most part, we paraffin oil but I am unhappy with it
because
it is too think and too frequently there is break in the oil and the
drop
dries too much. I find vacuum grease to be not terribly practical
because
it takes too much time - particularly when the well needs to be opened
(seeding, modifying well content, etc).
In ideal world I would like to find a much thicker oil that 1)
contains as
little volatiles as paraffin oil, and 2) allows no more water vapor
diffusion through it than paraffin oil. Hampton suggests Cargille
immersion
oils for this purpose but the MSDS for these oils states that they have
slight odor, so I am a bit concerned with unknown volatiles getting into
crystallization drop.
So, what do you like to use? Thanks much,
- Dima
--
Arun Malhotra Phone:
(305) 243-2826
Associate Professor Lab:
(305) 243-2890
Dept. of Biochemistry& Molecular Biology Fax: (305) 243-3955
University of Miami School of Medicine
PO Box 016129 E-Mail:
malho...@miami.edu
Miami, FL 33101 Web:
http://structure.med.miami.edu
