I remember a technique that used warmed Vaseline (liquefied) in a shallow pan. People would invert the plate and dip it into the liquefied Vaseline, then flip it over to dry. I suppose one could use masking tape to cover the outer edge of the plate to keep it Vaseline free. I am not sure how it would work with cryschem (24-well sitting drop) plates.
Good luck! Bryan -------------------------------------------------------------------------- Confidentiality Notice: This message is private and may contain confidential and proprietary information. If you have received this message in error, please notify us and remove it from your system and note that you must not copy, distribute or take any action in reliance on it. Any unauthorized use or disclosure of the contents of this message is not permitted and may be unlawful. -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of malho...@miami.edu Sent: Friday, November 18, 2011 1:26 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] sealing slides on VDX plates? Vaseline (pure, with no additives) from CVS/Walgreens in a 10 ml syringe and a pipette tip (wide bore) also works very well. On 11/18/11 12:58 PM, Bosch, Juergen wrote: > Dow Corning Heavy Vacuum Grease, a 10 ml syringe and a shorter cut 200 > µl tip attached to the syringe. > > Jürgen > > On Nov 18, 2011, at 12:55 PM, Dima Klenchin wrote: > >> Hello, >> >> I wonder what everyone is using for sealing hanging drop slides on VDX >> plates? For the most part, we paraffin oil but I am unhappy with it >> because >> it is too think and too frequently there is break in the oil and the >> drop >> dries too much. I find vacuum grease to be not terribly practical >> because >> it takes too much time - particularly when the well needs to be opened >> (seeding, modifying well content, etc). >> >> In ideal world I would like to find a much thicker oil that 1) >> contains as >> little volatiles as paraffin oil, and 2) allows no more water vapor >> diffusion through it than paraffin oil. Hampton suggests Cargille >> immersion >> oils for this purpose but the MSDS for these oils states that they have >> slight odor, so I am a bit concerned with unknown volatiles getting into >> crystallization drop. >> >> So, what do you like to use? Thanks much, >> >> - Dima > -- Arun Malhotra Phone: (305) 243-2826 Associate Professor Lab: (305) 243-2890 Dept. of Biochemistry& Molecular Biology Fax: (305) 243-3955 University of Miami School of Medicine PO Box 016129 E-Mail: malho...@miami.edu Miami, FL 33101 Web: http://structure.med.miami.edu
