Hi Theresa, We use polymerization by incomplete primer extension (PIPE). it can use very low (~1.5ng) of template so we don't have to use dpn1 after the reaction. link<http://www.springerlink.com/content/j02438u4434j257l/#section=92209&page=1>to paper.
Brett 2012/4/17 Xun Lu <xlun...@gmail.com> > Hi, Theresa, > > Our lab uses Phusion high-fidelity DNA polymerase. It might not be better > though. > > > > Regards, > > Xun > > > > Sent from my iPad > > On Apr 17, 2012, at 5:53 PM, Theresa Hsu <theresah...@live.com> wrote: > > > Dear all > > > > I would like to get some opinions on site-directed mutagenesis. What are > the current methods available? I know the Quick Change, are there others > that work better? > > > > Thank you. >
