increase your PEG3350 >27% and keep the other components at their current concentration. You can also add glycerol or ethylene glycol into the mix.
If you have multiple crystals then try various variants. Jürgen On Aug 23, 2013, at 1:52 PM, Uday Kumar wrote: Hello Can anyone suggest a cryoprotectant for the following crystallization condition 0.2-0.4M sodium formate ~20% PEG 3350 0-25 mM Nickel 0-100 mM Malonate Thank you with regards uday ...................... Jürgen Bosch Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Office: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-2926 http://lupo.jhsph.edu
