I've had good success transferring crystals grown in 200 mM NaOAc pH 5.5 / 10% PEG
Into a solution for room temperature diffraction (in ~6 steps, peg first, then buffers) : 100 mM NaOAc / 100 mM Malonate / 30% PEG And from there into a cryo solution in 4-5 steps) that was: 100 mM NaOAc / 100 mM Malonate / 30% PEG / 20% Ethylene Glycol Crystals have been solved to at least 1.4A resolution with mosaicity in the 0.2-0.3 range. -Alex On Aug 23, 2013, at 10:52 AM, Uday Kumar <u.ku...@fz-juelich.de> wrote: > Hello > > Can anyone suggest a cryoprotectant for the following crystallization > condition > > 0.2-0.4M sodium formate > > ~20% PEG 3350 > > 0-25 mM Nickel > > 0-100 mM Malonate > > Thank you > > with regards > uday
