Dear uday, I have grown crystals in 0.2M sodium formate and 20% peg3350, i tried cryo solution cotaining 0.2M sodium formate and peg1050 40%, it worked well for me and diffracted to 2.3A at home source. 0.2-0.4M sodium formate
40% PEG 1050 0-25 mM Nickel 0-100 mM Malonate On Fri, Aug 23, 2013 at 11:46 PM, Alexander D. Scouras <scou...@berkeley.edu > wrote: > I've had good success transferring crystals grown in > > 200 mM NaOAc pH 5.5 / 10% PEG > > Into a solution for room temperature diffraction (in ~6 steps, peg first, > then buffers) : > > 100 mM NaOAc / 100 mM Malonate / 30% PEG > > And from there into a cryo solution in 4-5 steps) that was: > > 100 mM NaOAc / 100 mM Malonate / 30% PEG / 20% Ethylene Glycol > > > Crystals have been solved to at least 1.4A resolution with mosaicity in > the 0.2-0.3 range. > > -Alex > > > > > On Aug 23, 2013, at 10:52 AM, Uday Kumar <u.ku...@fz-juelich.de> wrote: > > > Hello > > > > Can anyone suggest a cryoprotectant for the following crystallization > condition > > > > 0.2-0.4M sodium formate > > > > ~20% PEG 3350 > > > > 0-25 mM Nickel > > > > 0-100 mM Malonate > > > > Thank you > > > > with regards > > uday > -- ravi kumar
