Dear Thomas,
Alternatively you can try shooting on crystals in the drop, in situ. So
fishing, no cryo. But potentially high radiation damage. Can be
considered if you have enough crystals, and if your crystallization
plate makes it possible.
Regards
JL
On 14/08/2018 20:58, Thomas Krey wrote:
Dear crystallization experts,
We have 3D protein crystals grown from a microseed matrix screening
vapor diffusion experiment in either
15% (v/v) Reagent alcohol
HEPES Na pH 7.5
0.2 M MgCl2
or in
27% Isopropanol
0.18 M MgCl2
90 mM HEPES Na pH 7.5
10% Glycerol
Upon opening the corresponding wells these crystals move quite a bit –
presumably due to the volatility of the alcohols. Does anyone have a
good suggestion to stabilize the swirling movements? Does anyone have
experience, whether these conditions alone can serve as
cryo-protectant (i.e., do we really have to fish, move into cryo
solution and fish again)?
Any suggestion or input would be highly welcome.
Thank you very much in advance.
Thomas
Prof. Dr. Thomas Krey
Hannover Medical School
Institute of Virology
Structural Virology Group
Carl-Neuberg-Str. 1
D-30625 Hannover
phone: +49 (0) 511 - 532 4308
email: krey.tho...@mh-hannover.de
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