Hi Chitra, To add to the discussion, I can offer an example about obtaining the structure of a flexible/disordered N-term of a membrane protein.
Previous structures of the full-length multidrug efflux transporter AcrB (12 TM helices in each protein ~1000 residues, forms a trimer, so 36 TM helices) were missing the first 6 residues in the N-term in the cytoplasm but we could determine this structure and look at some interesting sequence-structure implications of these first 6 residues in our structure: "Crystal structure of the multidrug efflux transporter AcrB at 3.1 Å resolution reveals the N-terminal region with conserved amino acids Debanu Das,* Qian Steven Xu,* Jonas Y. Lee, Irina Ankoudinova, Candice Huang, Yun Lou, Andy DeGiovanni, Rosalind Kim, and Sung-Hou Kim" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2023878/ I think there should also be examples of C-term or N-term expression/purification tags that are ordered in some crystal forms of a target but disordered in other crystal forms/structures of the same target/homologs. Best, Debanu -- Debanu Das On Thu, Mar 12, 2020 at 5:02 AM chitra latka <chitra.la...@gmail.com> wrote: > Dear Klemens, > > I am going to setup the crystallisation of the entire protein anyhow. I > hope I get lucky :) > > Thanks > Chitra > > On Thu, Mar 12, 2020 at 5:12 PM Klemens Wild < > klemens.w...@bzh.uni-heidelberg.de> wrote: > >> On 12.03.20 08:53, chitra latka wrote: >> >> Dear All, >> >> I am working on a protein that has flexible C terminus. None of the >> available structures even in homologs have density for C term region >> (around 20 odd residues). All the available pdb entries have missing >> density for these 20 residues at C terminus. >> >> I am going to try my luck crystallising the entire protein in hope of >> getting density for C term residues as well (Fingers crossed). >> >> Has anyone faced a similar problem where they have managed to get density >> for a flexible terminus successfully? >> >> Any suggestions would be appreciated. >> >> Cheers ! >> >> Chitra Latka >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> >> Dear Chitra >> >> I would nevertheless try. Sometimes flexible termini fold back either in >> cis or in trans (crystal packing, a case I just had Yesterday) and you >> might learn sth important for biological regulation if you are lucky. At >> the same time I would truncate the terminus and crystallize the globular >> domain in parallel. >> >> Good luck >> >> Klemens >> > > > -- > Regards > Chitra > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
