Hi Aura,

1. What do you mean by “loading Matlab 8.2”? It should be Matlab 8.4 (2014b).
2. “Some still did”: what error did you get?

- Also, I’m confused by what you wrote, that subfields worked but hippocampal 
module didn’t (aren’t they the same thing?)
- Finally: can you please send us a screenshot of one of those abruptly cut 
hippocampi, so I better understand what might be going on?

Cheers,

/Eugenio


--
Juan Eugenio Iglesias
ERC Senior Research Fellow
Translational Imaging Group
University College London
http://www.jeiglesias.com
http://cmictig.cs.ucl.ac.uk/


From: <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of "Hurtado, Aura 
Maria" <ahurt...@mgh.harvard.edu>
Reply-To: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
Date: Wednesday, 21 March 2018 at 22:24
To: "freesurfer@nmr.mgh.harvard.edu" <freesurfer@nmr.mgh.harvard.edu>
Subject: [Freesurfer] Hippocampal Subfields Module, output error

Hi,

I am attempting to use the longitudinal pipeline of the "hippocampal subfields 
and nuclei of amygdala”, but keep getting a flawed output.

My dataset consists of images from a longitudinal study with some dropouts. 
First, I ran the reconall longitudinal pipeline in fs60 for all subjects. I got 
no errors. Then, I tried

1. Running the longitudinal pipeline of the hippocampal module, loading Matlab 
8.2 to run
segmentHA_T1_long.sh SUBJ1_basetemplate
segmentHA_T1_long.sh SUBJ2_basetemplate
etc

I got an error in most of these jobs (mostly for templates of subjects with 
multiple visits) that read

"ERROR: MatlabObjectArray(0x7fd7ce84cea0): Trying to access non-existing object 
(25)

Error in kvlGetOptimizer (line 11)

Error in SegmentSubfieldsT1Longitudinal (line 1155)”

2. I then tried running the cross sectional pipeline for each one of the 
timepoints in subjects already processed by the reconall longitudinal pipeline 
(i. e. segmentations in the  .long. directories)
segmentHA_T1.sh SUBJ1_timepoint1.long.SUBJ1_basetemplate
segmentHA_T1.sh SUBJ1_timepoint2.long.SUBJ1_basetemplate
segmentHA_T1.sh SUBJ2_timepoint1.long.SUBJ2_basetemplate
segmentHA_T1.sh SUBJ3_timepoint1.long.SUBJ3_basetemplate
etc

Most of them ran without errors, but some still did.

3. I then tried to run the cross sectional hippocampal module for all 
subjects/timepoints without using the reconall longitudinal pipeline
segmentHA_T1.sh SUBJ1_timepoint1
segmentHA_T1.sh SUBJ1_timepoint2
segmentHA_T1.sh SUBJ2_timepoint1
segmentHA_T1.sh SUBJ3_timepoint1
etc

They were processed without errors. But when inspecting the processing log, 
there was a message that appeared 4 times in the file

"WARNING: Registration did not converge in 5 steps!
*          Problem might be ill posed.
*          Please inspect output manually!
………
……..
…...
Everything done!
It took 766.0135 seconds “

With manual inspection, subfield segmentations output by fs60 seemed fine in 
all the directories of the reconall cross sectional and longitudinal outputs 
(hippoSfLabels-T1). For the Hippocampal Module outputs (hippoAmygLabels-T1), 
the hippocampus was abbruptly cut, sometimes laterally, sometimes dorsally. 
This was the case for all three analyses.

I wonder what is the problem and what I can do to fix it so I can successfully 
run the longitudinal pipeline of the "hippocampal subfields and nuclei of 
amygdala”


Thanks for your help!

Aura M.





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