Map the diffusion data to the subfield space, eg,
mri_vol2vol --mov fa.nii.gz --reg register.lta --targ subfields.mgz --o fa-in-subfields.mgz
Then run mri_segstats
mri_segstats  --ctab-default --i fa-in-subfields.mgz --seg subfields.mgz --sum fa-in-subfields.dat


On 1/12/2024 4:53 PM, Mirsol Choi wrote:

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Hi there,

Is there a way to successfully obtain FA/MD values from the hippocampus subfields?

Thank you,
M

On Fri, Jan 12, 2024 at 2:23 PM Douglas N. Greve <dgr...@mgh.harvard.edu> wrote:

    I'm not sure what you mean. The subfields are an ROI-based
    analysis. Are you saying you want to do a voxel-based analysis
    (without reference to ROIs)?

    On 1/9/2024 12:05 PM, Mirsol Choi wrote:

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    Hi there Freesurfer,

    I am currently using freesurfer to take DTI metrics (FA, MD) from
    hippocampal subfields.
    At this point of my exploration with freesurfer, I am able to
    correctly register the hippocampal subfields and diffusion data,
    and obtain my FA values from the subfields. However, this is at
    the individual subject level.

    I will eventually need to register all of my samples in the same
    space for me to do a group analysis. However I am unsure what the
    order of registration should be for this process to be feasible.

    Does this order seem feasible for accurate analysis?

    1) recon-all all of my subjects
    2) dt_recon all of my subjects (one of the outputs is a
    registration file, which has registered the DTI data to outputs
    of recon-all)
    3) run the hippocampus segmentation command on all subjects
    (segmentHA_T1.sh) to obtain hippocampal subfield segmentation
    4) register the segmentation file to the FA maps (output of
    dt_recon) using vol2subfield command
    5) After creating a subfield-FA map for each subject, spatial
    normalize the samples onto the same space using *mri_vol2vol*
    *
    *
    Thank you so much for taking the time reading my question. If
    there is a different method to spatially normalizing my datasets,
    or if there is a more optimal order of registration, I'd be happy
    to hear from you.

    Thank you,
    M

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