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Hi there,


Following the first registration step, should I also run another
registration command to put all fa-in-subfields.mgz for each subject onto
the same space for inter subject analysis of FA values?

Thanks again,
M

On Fri, Jan 12, 2024 at 3:00 PM Douglas N. Greve <dgr...@mgh.harvard.edu>
wrote:

> Map the diffusion data to the subfield space, eg,
> mri_vol2vol --mov fa.nii.gz --reg register.lta --targ subfields.mgz --o
> fa-in-subfields.mgz
> Then run mri_segstats
> mri_segstats  --ctab-default --i fa-in-subfields.mgz --seg subfields.mgz
> --sum fa-in-subfields.dat
>
>
>
> On 1/12/2024 4:53 PM, Mirsol Choi wrote:
>
>         External Email - Use Caution
> Hi there,
>
> Is there a way to successfully obtain FA/MD values from the hippocampus
> subfields?
>
> Thank you,
> M
>
>
> On Fri, Jan 12, 2024 at 2:23 PM Douglas N. Greve <dgr...@mgh.harvard.edu>
> wrote:
>
> I'm not sure what you mean. The subfields are an ROI-based analysis. Are
>> you saying you want to do a voxel-based analysis (without reference to
>> ROIs)?
>>
>> On 1/9/2024 12:05 PM, Mirsol Choi wrote:
>>
>>         External Email - Use Caution
>>
>> Hi there Freesurfer,
>>
>> I am currently using freesurfer to take DTI metrics (FA, MD) from
>> hippocampal subfields.
>> At this point of my exploration with freesurfer, I am able to correctly
>> register the hippocampal subfields and diffusion data, and obtain my FA
>> values from the subfields. However, this is at the individual subject
>> level.
>>
>> I will eventually need to register all of my samples in the same space
>> for me to do a group analysis. However I am unsure what the order of
>> registration should be for this process to be feasible.
>>
>> Does this order seem feasible for accurate analysis?
>>
>> 1) recon-all all of my subjects
>> 2) dt_recon all of my subjects (one of the outputs is a registration
>> file, which has registered the DTI data to outputs of recon-all)
>> 3) run the hippocampus segmentation command on all subjects
>> (segmentHA_T1.sh) to obtain hippocampal subfield segmentation
>> 4) register the segmentation file to the FA maps (output of dt_recon)
>> using vol2subfield command
>> 5) After creating a subfield-FA map for each subject, spatial normalize
>> the samples onto the same space using *mri_vol2vol*
>>
>> Thank you so much for taking the time reading my question. If there is a
>> different method to spatially normalizing my datasets, or if there is a
>> more optimal order of registration, I'd be happy to hear from you.
>>
>> Thank you,
>> M
>>
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