Hello,

Apologies for the delay in replying. The Table Browser will retrieve 
sequence corresponding to the items in whichever track you have 
selected. Thus even if you specify a region, the Table Browser returns 
the sequence for the nearest track item overlapping with your region.

To retrieve sequence for one exact region you can use our getDNA tool 
(accessible via the "DNA" link in the top bar above the main browser 
display):

http://genome.ucsc.edu/cgi-bin/hgc?g=getDna

If you have multiple regions the best thing would be to make a custom 
track of the regions and then use the Table Browser to retrieve the 
sequence. For detailed instructions on this method please see this 
previously answered mailing list question:

https://lists.soe.ucsc.edu/pipermail/genome/2006-September/011647.html

Hopefully this resolves the discrepancy from your second question. If 
not, we will need more information regarding how you obtained each of 
the conflicting sequences to be able to understand the issue.


Regards,

Pauline Fujita
UCSC Genome Bioinformatics Group
http://genome.ucsc.edu


> We have a couple of questions:
> 
> 1) In Table Browser if we request a sequence of a short genomic region of
> about 1000bp, Table Browser returns a much longer sequence and the
> coordinates are off. Is there any way to retrieve the exact stretch of
> sequence that we want?
> 
> 2) We retrieved promoter sequences for TNFalpha from both NCBI sequence
> viewer and UCSC Table Browser and they do not match. They are both hg19.
> What is the basis for this discrepancy?
> 
> Thank you!
> 
> _______________________________________________
> Genome maillist  -  [email protected]
> https://lists.soe.ucsc.edu/mailman/listinfo/genome

_______________________________________________
Genome maillist  -  [email protected]
https://lists.soe.ucsc.edu/mailman/listinfo/genome

Reply via email to