Hello Susan, You can either install BLAT and run it locally:
http://genome.ucsc.edu/FAQ/FAQblat#blat3 Or you can use a script to time your queries appropriately. One of our engineers has developed such a script - you can read a bit more about it in this previously answered mailing list question: https://lists.soe.ucsc.edu/pipermail/genome/2006-November/012216.html Hopefully this information was helpful and answers your question. If you have further questions or require clarification feel free to contact the mailing list at [email protected]. Regards, Pauline Fujita UCSC Genome Bioinformatics Group http://genome.ucsc.edu On 04/13/11 08:00, Lagerstedt, Susan A. wrote: > Hello, > > I received the following message. > > There is a very high volume of traffic coming from your site (IP address > 129.176.151.11) as of Wed Apr 13 07:52:37 2011 (California time). So > that other users get a fair share of our bandwidth, we are putting in a > delay of 10.2 seconds before we service your request. This delay will > slowly decrease over a half hour as activity returns to normal. This > high volume of traffic is likely due to program-driven rather than > interactive access, or the submission of queries on a large number of > sequences. If you are making large batch queries, please write to our > [email protected] public mailing list and inquire about more efficient > ways to access our data. If you are sharing an IP address with someone > who is submitting large batch queries, we apologize for the > inconvenience. Please contact [email protected] if you think this > delay is being imposed unfairly. > > Yes, I am guilty I submitted several file upload requests for BLAT at > once. > > I have 1400 120bp sequences that I would like to run through BLAT. > > Is there a quick and efficient way to do this. > > Thanks, > > > Susan Lagerstedt | Department of Laboratory Medicine and Pathology | > Development Technologist | Ph: 507-284-4844 | [email protected] > <[email protected]%20> | > Mayo Clinic | 200 First Street SW | Rochester, MN 55905 | > www.mayoclinic.org > > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
