Hi Mica, Most haematoxylins will do eg Harris or Mayer's. I would recommend a light counterstain (it is a counterstain, not the main stain). Differentiate if too strong.
Also periodic acid treatment increases nuclear affinity for Hx so shorten the counterstain time. Regards, Tony ________________________________________ From: Mica [faith14...@aol.com] Sent: Tuesday, 2 June 2015 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS stain Just a question...what kind of hematoxylin do you use as a counter stain for your PAS stain and do you use a differentiator? Sent from my iPad _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
