Spokane Ray points out something I've wondered about for years - can just anybody spit on the slide and remove the glycogen? I've never heard of any variation, but the number of people I've asked is very limited. This reference: http://www.ncbi.nlm.nih.gov/gene/276 certainly suggests that different people have different salivary alpha amylase activity.
Bob Richmond On Thu, May 5, 2016 at 2:27 PM, <koelli...@comcast.net> wrote: > I love having the Samuri Pathologist on this forum for wisdom and > real-laboratory life knowledge. And yes, I have in the past spit on slide > ON OCCASSION when faced with a dire necessity. Although I know there are > those who would wretch about this; it remains a fact of viable laboratory > life for some. > > My problem now is that in this era of (MUCH TOO MUCH) regulation, how do > you "test lots" or control from "lot-to-lot variation" in this SOP? When > Jane or Joe do this routinely and then goes on vacation, what about Sally > or Jim spit? There is a variation in copy number of the AMY1 gene > (amylase) and resulting difference in amylase protein concentration amongst > individuals. > > Why not just standardize it from the start, reagent, pH, temperature and > it really cannot fail. > > Spokane Ray > > ------------------------------ > *From: *"Bob Richmond via Histonet" <histonet@lists.utsouthwestern.edu> > *To: *"Histonet@lists.utsouthwestern.edu" < > histonet@lists.utsouthwestern.edu> > *Sent: *Thursday, May 5, 2016 11:10:40 AM > *Subject: *Re: [Histonet] PAS Stain > > > Amylase (diastase) for the PAS stain queries: > > Whatever happened to spitting on the slide (30 min at room temperature)? > John Kiernan advises "thinking of lemons and drooling into a small beaker" > though I'd advise chewing on a rubber band for a few seconds. > > He notes that alpha amylase is preferred. I'd go with the cheapest one in > the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma > offers a heat-stable alpha amylase. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
