We switched to alpha amylase since the diastase we were ordering was not working. We use a 2% solution of alpha amylase for 40 minutes at room temperature and it has been working fine.
Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -----Original Message----- From: Joanne Clark via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, May 04, 2016 4:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS Stain Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase method. We have been digesting the tissue in 0.5% diastase of malt in a 60 degree oven for 30 minutes, but do not see the glycogen being digested out. I have tried alpha amylase and beta amylase also without any luck. Does anyone have any suggestions to get the digestion to work Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
