Hi, Dear perl experts: I am a new hand of perl. I use perl because I am trying to use a package called "miRDeep<http://www.mdc-berlin.de/en/research/research_teams/systems_biology_of_gene_regulatory_elements/projects/miRDeep/index.html>". I install the *activeperl 5.10 for windows*. Then I used the MS DOS to try perl.
But there is an error coming out when I tried to run this command their demo: "perl auto_blast.pl 454_aligned.fa precursors. fa-b > signatures". The error message is as follows: *Copying subject file 'cp' is not recognized as an internal or external command, operable program or batch file. formatting subject file [NULL_Caption] ERROR: Could not open blast_dir_temp/file_subject Blasting query file against subject file [NULL_Caption] WARNING: readdb: wrong version of formatdb was used to make database blast_dir_temp/file_subject. [NULL_Caption] WARNING: bartel_100023_x1: readdb: wrong version of formatdb was used to make database blast_dir_temp/file_subject. Parsing blast output: 'cat' is not recognized as an internal or external command, operable program or batch file. 'cat' is not recognized as an internal or external command, operable program or batch file. 'cat' is not recognized as an internal or external command, operable program or batch file. 'rm' is not recognized as an internal or external command, operable program or batch file. 'rm' is not recognized as an internal or external command, operable program or batch file. * But I think the perl code "*auto_blast.pl*" is tested maturely by the author of the package so I am suspicious that there may be something wrong with my system? The *MS DOS is 5.1.2600 by windows XP*. I wonder if the above error message due to this or other reasons? I really appreciate if someone can point me out of this and give me a clue of the problem. Thank you very much for your time. Sincerely, Fei
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