Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block
Exactly, I process the agar,not the tissue in it Cells,organoids, spheroids. Bernice -Original Message- From: Colleen Forster via Histonet Sent: Friday, May 17, 2024 3:07 PM To: Jay Lundgren Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block Chistopher, Were these small pieces of tissue? If they are in an agar (such as histogel) they would need to have been processed overnight to ensure the agar is completely dehydrated during processing. The sample, no matter how small, is protected and processes perfectly. IF you ran a short run with the agar it will not have [processed properly. I was never able to salvage those samples. It took me a couple times to figure out the solution. Just a thought. Colleen Forster HT(ASCP)QIHC On Fri, May 17, 2024 at 2:32 PM Jay Lundgren via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Where did the agarose come from? > > On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > > > > > Hello everyone! > > > > I'm having trouble sectioning tissue array blocks where the array > > is in agarose embedded into a paraffin block. I've chilled the > > blocks and I'm sectioning on a rotary microtome, at 5 microns, with > > a high profile Accuedge blade. The paraffin surrounding the agarose > > sections normally, > but > > the agarose portion of the block causes the blade to "skip" across > > it slightly and even chip out as if my blade isn't snug in the blade > > holder (it is). If I do get a tiny portion of agarose on my section > > to float > out > > on the waterbath it flies away (like adding ETOH to a waterbath with > > sections already on it.) Everyone I have asked about this says the > > agarose should section normally with the paraffin like any other > > FFPE blocks. Any ideas on why this agarose is behaving this way for > > me? Thank you in advance! > > > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/ > > listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SEM0o5vbbKuzHCJUAy39eR > > ALW6YwlWuJkF7rTMW9nJ_DRMWtgXqBRmD4yuNChwCHd4H_uCmrPk4dybLFdX60UH_YOK > > Z8ESaaEB3125Zq$ > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SEM0o5vbbKuzHCJUAy39eRALW6 > YwlWuJkF7rTMW9nJ_DRMWtgXqBRmD4yuNChwCHd4H_uCmrPk4dybLFdX60UH_YOKZ8ESaa > EB3125Zq$ > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SEM0o5vbbKuzHCJUAy39eRALW6YwlWuJkF7rTMW9nJ_DRMWtgXqBRmD4yuNChwCHd4H_uCmrPk4dybLFdX60UH_YOKZ8ESaaEB3125Zq$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Golgi-cox staining
From: Rizaldy P Scott Sent: Wednesday, April 10, 2024 4:33 PM To: Bernice Frederick Subject: Re: [Histonet] Golgi-cox staining Hi Bernice, What they should have done ideally is to store the uncut agarose blocks submerged in whatever buffer they used to rinse the brains out prior to agarose embedding. They can probably salvage the dried-out brains by rehydrating in their buffer or 30% sucrose solution overnight (or store at 4 degrees as needed) and re-embed in agarose only when they are ready to cut. I can't guarantee however if artefacts due to drying might result but it's worth the shot. Best regards, Riz Rizaldy P. Scott, M.S., Ph.D. Research Associate Professor of Pathology Scientific Director Mouse Histology & Phenotyping Laboratory Robert H. Lurie Comprehensive Cancer Center Northwestern University Feinberg School of Medicine Olson Pavilion, Room 8-335, 710 N. Fairbanks Ct., Chicago, IL 60611 ✉️ rizaldy.sc...@northwestern.edu<mailto:rizaldy.sc...@northwestern.edu> +1-(312)-503-2695 https://www.feinberg.northwestern.edu/sites/mhpl/ www.cancer.northwestern.edu<http://www.cancer.northwestern.edu> ________ From: Bernice Frederick mailto:b-freder...@northwestern.edu>> Sent: Wednesday, April 10, 2024 13:40 To: Rizaldy P Scott mailto:rizaldy.sc...@northwestern.edu>> Subject: FW: [Histonet] Golgi-cox staining Can you help this person? Bernice -Original Message- From: Mariela Chertoff via Histonet mailto:histonet@lists.utsouthwestern.edu>> Sent: Wednesday, April 10, 2024 9:28 AM To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> Subject: [Histonet] Golgi-cox staining Hi all We made the Golgi cox staining and due to a problem with the vibratome, we left the tissue several days embedded in agarose and they get dryed, It is possoble to recover the brains? it is better to repeat the agarose embebbing o it is better to put the brains in crioprerervate solution to rehidrated and after that put them in agarosa again? We are following the Zaquot protocol https://urldefense.com/v3/__https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848hoVaCzp$<https://urldefense.com/v3/__https:/www.frontiersin.org/articles/10.3389/fnana.2016.00038/full__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848hoVaCzp$> Thanks in advance for your reply Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Química Biológica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabellón II Piso 4 Ciudad Autónoma de Buenos Aires C1428EGA - Argentina Tel: 54 11 5285-8680/1/2 email:marielachert...@gmail.com marielachert...@qb.fcen.uba.ar<mailto:marielachert...@qb.fcen.uba.ar> mailto:mariela.chert...@uab.cat>> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848ty2UFsq$<https://urldefense.com/v3/__http:/lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848ty2UFsq$> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] preordering giemsa stains
We used to Alcian Yellow... Bernice -Original Message- From: Gudrun Lang via Histonet Sent: Thursday, February 29, 2024 8:48 AM To: 'Paula' Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] preordering giemsa stains In my place it is ordered on demand, but the pathologists usually perfer IHC for Hp. Gudrun -Ursprüngliche Nachricht- Von: Paula via Histonet [mailto:histonet@lists.utsouthwestern.edu] Gesendet: Dienstag, 27. Februar 2024 20:46 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] preordering giemsa stains Hello, Is it a common practice to preorder stains that involve the stomach searching for H pylori? Or, is it more common to order as needed? Thank you for anyone's feedback. Paula Lucas Lab Manager ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!X7dgilKf4-E3EhKyDtv4fhEtpCU8Wn2T2DDP5BXwZz1QBzrRzg4fSvYjkrZlIXOzt4OeVj_obLymNiZvAoblSsCoe5C7VBQN6GFIavSx$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!X7dgilKf4-E3EhKyDtv4fhEtpCU8Wn2T2DDP5BXwZz1QBzrRzg4fSvYjkrZlIXOzt4OeVj_obLymNiZvAoblSsCoe5C7VBQN6GFIavSx$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Von Kossa staining
I use a 60 watt bulb and it works fine. Bernice -Original Message- From: John Kiernan via Histonet Sent: Wednesday, July 26, 2023 11:51 PM To: Charles Riley ; histonet@lists.utsouthwestern.edu; dsiena Subject: Re: [Histonet] Von Kossa staining Charles, A handheld light of any kind isn't really suitable because you would have to hold it over the slides for 15 to 60 minutes, according to which variant of the von Kossa method you plan to use (see Lillie & Fullmer 1976 Histopathologic Technic ... 4th ed. pp 539-541). An anglepoise lamp with an old-fashioned 100W bulb is OK, and so is a sunny windowsill. Silver salts absorb at the blue end of the spectrum, so a fluorescent light should be more efficient than an incandescent bulb. If no bright light source is available, it's possible to chemically reduce the silver phosphate and/or carbonate to black colloidal silver, with a traditional photographic developer. The method of Rungby et al.1993 may be better than other post-reduction methods (https://urldefense.com/v3/__https://scholar.google.ca/scholar?hl=en_sdt=0*2C5=rungby*1993*calcium*deposits=rungby*1993__;JSsrKys!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfChgoE5Zi$ ). I never tried it, but Rungby's paper has collected 104 citations, which is very good for a paper in our field. The von Kossa technique is simply explained in my Histological and Histochemical Methods textbook, 5th edn (2015). The book costs less than 1ml of any antibody. Enough said! John John A. Kiernan Emeritus, Anatomy & Cell Biology, University of Western Ontario London, Canada https://urldefense.com/v3/__https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html__;!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfCiLlysL2$ Also Secretary, Biological Stain Commission, Inc. https://urldefense.com/v3/__https://biologicalstaincommission.org__;!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfCtBsy2U8$ = = = -Original Message- From: Charles Riley via Histonet Sent: Wednesday, July 26, 2023 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Von Kossa staining *** Externally sourced email message *** Can anyone out there who performs Von Kossa staining provide me with any guidelines or suggestions for the light source to use for the Silver nitrate activation? Is a standard handheld black light strong enough or does it need to be a UV sanitizing strength light if using UV versus incandescent bulb exposure? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfCgGN6_75$ CAUTION: This email was originated from outside StatLab and contains links. Do not click links or open attachments unless you recognize the sender and you know the content is safe. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfCgGN6_75$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfCgGN6_75$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] water under sectios
Good morning (well, in Chicago anyway) We've recently been having issues with water under the sections when picked up from the waterbath. It seems to even affect the sections during antigen retrieval after removing excess water, drying and baking I was given a block from an outside source that I know is a different paraffin and there was no water to be seen. Same person,same techniques. Can paraffin play a part? Or is the processing throwing this off. We run various programs and it seems to happen with almost everything. TMA"s in particular, as cores get lost during retrieval. Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center 710 North Fairbanks Court Olson 8-421 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RAt teeth
Hi all, I have bamboo rat teeth that have (ha-ha) been decalled. Obviously they are not soft. Can I use Nair on them or is there something better? I will need an H and an iron stain. They are not tiny samples.. Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center 710 North Fairbanks Court Olson 8-421 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P16
Who is the best and current supplier for p16? We were using biocare,but evidently it is no longer available from them. And no Santa Cruz per my IHC tech. Thanks, Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center 710 North Fairbanks Court Olson 8-421 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] sectioning issues?
You may have to call a tech at thermos to ask.. It still sounds like it it's the microtome as we know they cut. Join Histonet or the NSH facebook page and you can ask there as well. I may just be missing something obvious. histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> for histonet. It is a forum. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> From: Elizabeth Jane Lux Sent: Friday, April 21, 2017 10:04 AM To: Bernice Frederick Subject: Re: sectioning issues? Thanks so much for your help! Frustratingly having issues when I move into sectioning. Ribbons look square at trim mode (20u) but when I try to get my sections, I'm getting inconsistent slicing, almost like it's compressing it at times. Or I guess not advancing the specimen correctly? Perhaps the arm needs maintenance? Oh, and I was completely wrong on the model, it's a Thermo Finesse ME+ Liz From: Elizabeth Jane Lux Sent: Friday, April 21, 2017 8:58 AM To: Bernice Frederick Subject: Re: sectioning issues? Awesome! I'll bring the block(s) over now, thanks! Elizabeth Lux Research Tech 3 Feinberg Cardiovascular Research Institute Northwestern University Lurie Building, 10-220 312.503.6368 lab phone 312.503.0219 fax ____ From: Bernice Frederick Sent: Friday, April 21, 2017 8:55 AM To: Elizabeth Jane Lux Subject: RE: sectioning issues? You can always pop by and let me see. Now would be fine. I am gone all next week. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> From: Elizabeth Jane Lux Sent: Friday, April 21, 2017 8:27 AM To: Bernice Frederick Subject: Re: sectioning issues? Tissue is kidney and heart. Thickness was going off of past work done in the lab, utilizing the guidelines in the AFIP Lab methods in Histo red book. The machine had some maintenance done last year, though the tech seemed really green and unsure of himself. The block seems secure to the cassette. Thanks for your thoughts! Elizabeth Lux Research Tech 3 Feinberg Cardiovascular Research Institute Northwestern University Lurie Building, 10-220 312.503.6368 lab phone 312.503.0219 fax ________ From: Bernice Frederick Sent: Friday, April 21, 2017 7:43 AM To: Elizabeth Jane Lux Subject: RE: sectioning issues? Hi Liz, What kind of tissue are you sectioning? Bear in mind,the microtome you are using may have never had any maintenance on it. And why are you cutting at 6? Unless you are doing neuro or looking for amyloid, 4 um is the best thickness. The block itself may be coming away from the cassette. This can cause said issues. Wiggle the paraffin and see if it is not coming away from the cassette. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> From: Elizabeth Jane Lux Sent: Thursday, April 20, 2017 4:09 PM To: Bernice Frederick Subject: sectioning issues? Hey Bernice, Wonder if I could ask your opinion on some microtome troubleshooting. I'm having a few tricky paraffin blocks. Facing it gets me nice smooth ribbons, but when I move to the sections (6micron) I get inconsistent results. It will give me a full section, then a half, then full, then half, and so on . . Moved to a test block to work out the kinks, and adjusted the angle slightly, tightened up the blade holder, and things seemed to be ribboning better. But then still having issues with the sample block of interest. Anyway, thought it was worth asking if you had any advice. Thanks! Liz Elizabeth Lux Research Tech 3 Feinberg Cardiovascular Research Institute Northwestern University Lurie Building, 10-220 312.503.6368 lab phone 312.503.0219 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TUNEL
Hello all. Would there be any reason that slides stained with TUNEL (ISH) would not pick up the Mayers counterstain? We use it for IHC and it is fine. Mayers for 7 minutes and then ammonia water for 1 minute. We do that rather than rinse for 15 minutes and there are usually no issues. Could a reagent in the stain be causing it? DAPI is used... Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fibroblasts
Hello all, Don't have a ton of time to search so am asking. Any good markers specific to fibroblasts? And am drawing a blank for regular special stains for some reason. Researcher says Vimentin is not specific enough. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] floor vibration
We have issues as HVAC is above us! Sometimes at the switch over from heat to air and vice versa the facilities personnel don't check the drains and we get a flood. Came in one morning to hear dripping and we were going to a self -eval that day.! Most of the water was in my garbage can (Thankfully) Just missed my PC and the TMA arrayer. Got part of the embedding unit (it was a big leak). Facilities had the wet/dry vac up in the ceiling to get rid of the water. Had to have a good chunk of ceiling replaced and repaired after that one. There was even water in the light fixtures! Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: Jeffrey Robinson via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 5:08 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] floor vibration An additional word of caution about placing the EM Lab under ANY kind of potential water leak. Tim Morken and I can attest to that! They installed a new CT scanner in Radiology directly above the EM Lab. They broke some sort of water pipe during the installation and it leaked so much that part of the ceiling collapsed onto the scope! They didn't bother checking to see if there was any damage on the next floor down- they just left. Tim had a huge surprise waiting for him the next morning. We always had to cover the scope with a sheet of plastic at night after that in case they installed something else. Jeff Robinson, Senior Histotechnologist, EM Tech (emeritus), Sierra Pathology Lab, Clovis, CA. -Original Message- From: Keyser Gerald T via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 1:25 PM To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] floor vibration I can think of two things. First, relentlessly make fun of your building planner for putting a histology lab underneath a laundry. This is a mistake worthy of pointing and laughing. Second, there are isolation tables and platforms. That's probably the first thing I would try. http://www.taab.co.uk/pdf-details/305_taab_products_1402580607.pdf Gerry -Original Message- From: Nancy Schmitt via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 10:03 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] floor vibration Happy Monday! We are moving to a new space and part of our area is above the laundry - there is some vibration from there. Does anyone have any experience with this and could you please share how you accommodated this? Special flooring, pads, etc. Thank you much! Nancy Schmitt HT, MLT (ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mycoplasma
Hello all, Will PAS stain mycoplasma? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP
ANP21382. What kind of policy or SOP do you all have for this question? T asks how reagents are given an expiration. This includes but is not restricted to reagents where manufacturer does not specify a date. We date made up reagents as a 6 month expiration unless we know it doesn't last that long. Came up during interim self inspection. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD34
Been looking around a bit and seem to have it a roadblock. Does anyone know of a vendor for CD34 that will cross react with rabbit? On frozen sections, mind you. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil Red O
Hello all, Will Oil Red O stain phospholipids? We will be helping another lab with some work and the project out line states that Phopslipids are externalized on the cell surface during apoptosis. Can always try it I suppose. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Masson's on Frozens.
Question: When we stain and HE on frozen we fix in 95% prior to staining. As I never in 30 years have done a Masson's Trichrome on a frozen section I ask : do I need to fix and then mordant as usual or go straight to the Bouin's and continue as normal? Do I need to mordant at all? Tissue is rabbit liver. BErnice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipping mystery
We have a CV5030 and use the suggested Surgipath micromount. It works well. As has been previously mentioned, you can adjust the amount of mountant dispensed as desired. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: Caroline Miller [mailto:mi...@3scan.com] Sent: Saturday, July 11, 2015 9:18 AM To: John Kiernan Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 On Jul 10, 2015, at 10:55 PM, John Kiernan jkier...@uwo.ca wrote: DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. John Kiernan = = = On 09/07/15, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Trichrome troubleshooting
Actually, I have microwaved the Bouins (and still do) for small numbers of slides and the results are the same. I ran a liver bx both ways as well as larger tissue to compare. I use the 10 slide plastic coplin jar and generally have 5 or less slides when I do this. One microwaves the Bouins for 30 seconds on power level 7 in a lab grade microwave. A higher level will cause the Bouin's to spill. Slides are then added and left for 5 minutes. Excess rinsed out and then proceed as per your SOP. As for the blue- I rinse out excess and do 1 dip in 1% GAA. Rinse and dehydrate (10 dips each solution) Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: Elizabeth Chlipala [mailto:l...@premierlab.com] Sent: Tuesday, June 30, 2015 3:30 PM To: Suzanne Martin; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome troubleshooting Suzanne How many times have you used the kit and reagents, I did look up how the kit works but the trichrome stain can be tricky. First of all you need to make sure that the mordant (bouins solution) is at 60C prior to placing your slides in them. We normally heat up our bouins for at least an hour prior to placing the slides in the solution. We leave in bouins for an hour and a half rather than an hour. I see that this is a microwave protocol I cannot comment on that but I don't think that the hematoxylin is the issue, if you leave longer in 1% acetic acid that may pull some of the blue stain out or I would try dehydrating with lower alcohol percentage that can pull some of the blue stain out. I would also try leaving it a bit longer in the bouins after you microwave it - that might help. Trichrome staining works best with fresh reagents so if you have used these reagents too much that could cause problems. I'm also not a big fan of the one step trichromes, they are quicker but sometimes not as good as the two steps, just my opinion. FYI - to evaluate your staining look for a smaller vessel, the smooth muscle should be nice a red, if its greyish or blue you have not done the stain properly. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Suzanne Martin [mailto:smar...@lcpath.com] Sent: Tuesday, June 30, 2015 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome troubleshooting Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange. We have tried lessening the time in Gills, adding time for the last acid step, even lessening time and adding time in the Weigerts. Thoughts? Thank you. Suzanne HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] : Paraffin block disposal
All, Any blocks for patients on clinical trials should also be kept to the end of the trial (please flag them and keep them somewhere) or sent to the cooperative group as the patient did consent for use of their blocks (usually) for future use. As well, one block is not always sufficient as that person may end up on more than one trial and need a different block (like a LN when a tumor block was originally needed). Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: Mayer,Toysha N [mailto:tnma...@mdanderson.org] Sent: Monday, June 08, 2015 9:02 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] : Paraffin block disposal The regulation says two years. I was always led to believe that for Pedi, it should 10 years past the age of 18. Some facilities add the phrase 'past sign out' onto the policy for disposal. The methods can vary according to facility and state. In some places that could mean in the trash, in others biohazard waste. If confused check with another long standing facility and a newer one in the area to get an idea of what should be done. I have usually placed them in the biohazard trash, so that there would be no issues with anything. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 1 Date: Sat, 6 Jun 2015 10:24:42 -0700 From: Aimee Tolentino a.tolentin...@gmail.com To: Arbaugh, Roberta rarba...@csdermatology.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin block disposal Message-ID: 1f36aba5-452b-4556-8d1e-e5d09fdb2...@gmail.com Content-Type: text/plain; charset=us-ascii That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta rarba...@csdermatology.com wrote: Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Toluidine blue
Hello all, I was taught to do Toluidine Blue O without a control. Is there actual one and what would it be? I'm staining a bone core. Don's ask why, it's research and what a researcher wants... Plus they have a protocol they are following for this cartilaganous defect. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MTA-1 Microarrayer
Hello all, Anybody have any idea where to get the batteries for this arrayer? Regular 2450 batteries are too thick. Batteries plus was a no go. See some on Amazon but not sure if they are thin enough. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question
All, I have a procedure here that call for and I quote 1.25 ml NaOH 10N in 1L of water. I know how to get 1 N, but how do I get 10. Having rarely hd the opportunity to make many Normal solutions ,my brain is not computing. Is it an error? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Schiffs
Am I missing something? I ordered Schiffs and sigma tells me it was shipped yesterday. Hello, today is Friday and last I recall Schiffs need to be refrigerated! You'd.. think they would realize this. Sorry all, have to vent. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Schiffs
Having a brain fart all- does the Schiff go into the formaldehyde or vice versa to test if the Schiffs is still good? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Kidney Stone Histology?
Do you all see the picture of the stone on the NSH facebook page? It looks like an easter egg! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Andrews Sent: Saturday, April 04, 2015 10:54 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Kidney Stone Histology? What Everyone Else Wrote. They're stone (apatite; the kind I myself get) or hard crystals (uric acid). They're very unfriendly to a microtome blade. Getting a thin section of an apatite stone would require petrological thin-section techniques, which won't be in the repertoire of the typical soft-tissue histo lab. Think epoxy, abrasives, and time. Under a stereo scope, they can be amazing: covered with sharp corners and edges, and all the corners and edges have more corners and edges on them. For a big staghorn stone, a diamond rock saw might be required. Uric acid crystals aren't particularly better. But histo techniques pretty much don't apply. On Fri, Apr 3, 2015 at 1:42 PM, Norton, Sally sally.nor...@seattlechildrens.org wrote: The only thing we do with stones is send them to the Mayo clinic for chemical analysis. Sally Norton Seattle Children's -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Friday, April 03, 2015 11:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Stone Histology? How the heck do I process and section kidney stones? And what kind of stain do you like for them? Thanks Jerry Ricks Research Scientist University of Washington Department of Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- 73 de Mike Andrews W5EGO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cleaning glassware
We always acid clean before doing any silver stain. No metal Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, April 02, 2015 2:36 PM To: Jeff Halstead; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: cleaning glassware I work in a small veterinary lab and when I do the Warthin Starry stain I mix all the reagents in never used before disposable plastic beakers and I stain the slides in an un-used slide mailer. Roberta Horner Animal Diagnostic Lab Penn State University -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] BS in Histotechnology
They don't realize the theory we have to learn and those questions we have to answer like What's the best fixative for a pheochromocytoma? You tell them and they say the pathologist says B-5, to which I said, well they wouldn't pass out registry exam with that answer.Grrr. Or the difference between a Mucin, Pas and Alcian Blue. The cytopath who asked did really need to know. As well I vaguely recall a question back on my HTL exam asking why a pathologist would request a mucin stain Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 2:14 PM To: Carl Nituda; Marcum, Pamela A; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I know someone personally that works in a hospital and it hast Histotechnologist by his nameand he never took the HTL exam. He said his hospital bases it on experience From: Carl Nituda [cnit...@nvdermatology.com] Sent: Tuesday, March 24, 2015 2:32 PM To: Marcum, Pamela A; Sanders, Jeanine (CDC/OID/NCEZID); Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I personally think that a person just can't call themselves a Histotechnologist unless they went to school, training, and then pass the BOC by ASCP. Anyone, I mean anyone can perform a job with proper training in any field but that doesn't mean they should have that title until they pass certification. For hiring managers, I encourage you to hire certified candidates as priority and call them a Histotechnician, or Histotechnologist based on their certification. If a person is doing Histology work and is uncertified, encourage them to be certified and just don't give them a title. Imagine a world when people doing the job is actually certified like other professions, then you will get the respect from your colleagues that you deserve. Changes for the future of the profession starts with good leaders. Have a good and blessed week everyone. Carl Nituda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 24, 2015 10:53 AM To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology That was nicer than the pathologist who told me years ago, any monkey could be trained to do my job. I basically did not take the job I was interviewing for at the time. At least the next interview went a lot better and I did take the job. Pam -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 12:30 PM To: Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I agree, BUTas long as many pathologists think you can teach any trained dog how to section histology will never have the recognition those of us that have studied and trained deserve. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 24, 2015 12:59 PM To: Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration. In my opinion histologists are just as important and needed as MT. Even though there is an increase in automation in pathology the hands on of a histologists is most important. The fact that hospital still consider a lower entry job is the reason there are not more of us. It is quite frustrating. Sue TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list
RE: [Histonet] FW: Release of blocks to research facilities
Research facilities usually pay the submitting institution for submitting the block to the study. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Tuesday, March 17, 2015 10:45 AM To: Abbott, Tanya Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: Release of blocks to research facilities There are other issues, besides CAP, that you should consider when releasing blocks. 1. A block is a medical record. Has the patient consented to release for research? 2. If the block is released prior to 14 days discharge and there are any charges, the charge must be sent to your hospital. Post 14 days the charges are sent to insurance and patient. 3. Will the research lead to a commercial product? Again, written patient consent. 4. You may be able to charge the research facility for sending the block. Administrative costs. 5. Communicate with patient is a must. 6. Get written consent and have the receiving facility verify they will handle properly, retain or return the block d you will get copies of data for patient file. Make sure you reduce your risk. Sent from my iPhone On Mar 17, 2015, at 5:38 AM, Abbott, Tanya tanyaabb...@catholichealth.net wrote: I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. From: Abbott, Tanya Sent: Monday, March 16, 2015 3:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Release of blocks to research facilities CAP checklist ANP.12500 refers to Record Retention. I am looking specifically at NOTE 2: Regarding extra-institutional release of blocks for research purposes. I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. Thanks in advance for your help! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Old slides.
Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls:
There was actually an article written in The Journal of Histotechnology a few years back on this and it was, I f I recall, using a hot dog to create the gram control. Might be in the archives on nsh.org. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, March 05, 2015 9:21 AM To: Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: We have successfully used hamburger meat to make Gram controls. As far as GMS controls go, we ran across a post from someone smearing cream cheese onto lung tissue and letting it sit for a couple of days, fixed and processed it and were able to demonstrate Aspergillus by GMS. My fungus control stocks are low so I was actually planning to try this with some beef lung. I haven't heard of the Slim Jim method before. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Automatic H E slide stainer recommendations
I agree as well. The XL is a wokhorse. We have the transfer station and the CV5030 to go with it. Bernice Frederick (HTL) ASCP Senior Research Tech Pathology Core Facility Robert H. Lurie Cancer Center Northwestern University 710 North Fairbanks Court Olson Room 8421 Chicago, IL 60611 312-503-3723 b-freder...@northwestern.edu From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histot...@imagesbyhopper.com [histot...@imagesbyhopper.com] Sent: Friday, January 09, 2015 5:37 AM To: ian bernard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Automatic H E slide stainer recommendations I second the Leica XL. That stainer is a reliable workhorse. Sent from my iPhone On Jan 7, 2015, at 8:08 PM, ian bernard ian.bern...@comcast.net wrote: The Leica Autostainer XL has proven effective for our lab. IRB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, January 06, 2015 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H E slide stainer recommendations Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TMA arrayer
Hello all, If anyone has a semi-automated arrayer, where did you get it? We have one from Veridiam, but are wondering if anyone else makes them. We are looking to purchase another unit. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Specimen numbering systems
We do Alpha numeric so A1,A2 but our samples also get barcodes so we have a barcode ID that picks up whatever you designate it to and it is AAA001234 (designating a block) the slides then become AAA001234 0001, AAA001234 0002 etc. ties it back to the block and uniquely identified (called parent and children) The hitch here is if you had, say a colon it would be the (parent) and each block created would be a child and then technically the slides become the grandchildren. It works, but can be confusing at times - we're still working out the kinks. Turns out NCI and NIH use the system. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, November 21, 2014 2:11 PM To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems We are currently using Numeric for the specimen (or part) and Alpha for the block. I don't like it; we frequently have 1Z blocks for large CA resections! I would like to change this going forward. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, November 21, 2014 2:03 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Specimen numbering systems Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Flames at embedding centers
Darn right! We'll live forever at this rate of fixation... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, November 19, 2014 2:46 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Old Histotechs never die. They're just well fixed... :). From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Mark Turner [mtur...@csilaboratories.com] Sent: Wednesday, November 19, 2014 1:53 PM To: Sanders, Jeanine (CDC/OID/NCEZID); Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers We had a special clean counter we used for pizza on a regular basis. I worked with a pathologist who refused to wear gloves and would gross colons bare-handed. Guy is still alive and kicking at 85! In the very old days, we used carbon tetrachloride to dehydrate in the open tissue processor (Technicon). Not going to say anything at all about disposal Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:10 PM To: Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Okay, since we are confessing. When I was a student in histology school, we did the potluck thing IN THE LAB! I mean, ALL the food was laid out on a back counter -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, November 19, 2014 2:08 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Since the break room was right across the hall from the histology lab, we use to clear off a counter to put all the food for our potlucks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:04 PM To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers sigh the good old days -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Rubber mats for pinning specimens
We make up paraffin trays, either in a cafeteria tray (sssh) or in the lid of a slide box if it's for something small. Works like a charm and supplies are at hand. Besides that, paraffin floats in a formalin tank as well. You could have custom cut trays and still use your paraffin. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, November 18, 2014 10:21 AM To: Dennis Hahn; 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Rubber mats for pinning specimens Have you tried to pin on large cork sheets? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, November 18, 2014 8:18 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Rubber mats for pinning specimens Can anyone tell me if there is a company that sells the rubber mats for pinning open larger specimens, such as colons? We are currently using cooled paraffin as our pinning surface. I have found one company that sells small pans with the rubber already inside of it, but I'm looking for something we can cut to fit our current containers, no small specimen pans needed. The pathologists have also stated that they do NOT want cork. Thanks again, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histology Tracking systems
Our tracking system has everything there. We can create templates for various scenarios based on what is needed. The barcodes are generated by the system. It's a complicated system and takes a little time for data entry and such, but it does work. Our microscopy area is not set up in it as of yet, but it takes time. Once the requisition has been made we go in and make as completed for each task we perform. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Thursday, October 16, 2014 4:17 PM To: Morken, Timothy; 'Willis, Donna G.'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Histology Tracking systems We do about the same thing with the embedding and sectioning. The computers are touch screen and some people use them, others use the pad on the keyboard, the rest asked for mice. We have no way to get the information we need for sectioning from the system so, we have to do the paperwork by hand. The system we have was created by engineers who thought we should do processing, embedding and staining, then deliver the slides. They missed sectioning completely and say they can't add it now. If you are on check out you get credit for sectioning, staining and delivering every slide cut that day. Just doesn't work well for workload recording daily. Someday maybe they will ask the people doing the work instead of assuming we are CP. Nah, can't happen to easy. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 16, 2014 4:06 PM To: 'Willis, Donna G.'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Histology Tracking systems Donna, we have touch screens, but our application is not fully touch screen friendly yet, so we have mini-keyboards that include a trackpad. They work well for login, choosing some items on the screen etc. They are not really meant for extensive use. 99% of the interaction is achieved simply by scanning a block or slide and using the touch screen as much as possible. the keyboard is just for that one percent in which the screen does not work so well. We do all QA recording on paper at the microtome and embedding center, and then computer entry later. Again, the system is not set up well for that aspect yet. If you are thinking of entering barcodes manually (you can do that if you know the ID) , yes, you can do it, it just slows things down a lot. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, October 16, 2014 2:00 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Histology Tracking systems I know this might be a strange question but, do any Histology Labs out in Histoland use a keyboard and mouse at Embedding and Microtomy to perform tracking, slide label printing and Quality Assurance recording. Thanks in advance for your replies. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges
RE: [Histonet] On the lighter side...
Or we'll live forever. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Monday, August 11, 2014 12:49 PM To: Shirley A. Powell; Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Saves on embalming costs Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I agree. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjo...@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, (what¹s that in micron¹s?²) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 8/11/14, 5:17 AM, Edwards, Richard r...@leicester.ac.uk wrote: Sniffed my first formalin and saw first post-mortem November 1965. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
What's that in microns??? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Thursday, August 07, 2014 3:11 PM To: Douglas Porter; 'Baldridge, Lee Ann'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I have been recruiting exclusively in histology for 10 years. ; ) Thanks-Pam Right Place, Right Time, Right Opportunity. Pam Barker RELIA Solutions. rel...@earthlink.net (407) 353-5070 cell/text Sent from my Galaxy S®III div Original message /divdivFrom: Douglas Porter doug.por...@caplab.org /divdivDate:08/07/2014 4:10 PM (GMT-05:00) /divdivTo: 'Baldridge, Lee Ann' lhad...@iupui.edu, histonet@lists.utsouthwestern.edu /divdivSubject: RE: [Histonet] On the lighter side... /divdiv /divWell...I appears most of us are right properly fixed. Good to see some newbies chiming in. This also shows that we need to get/keep more of the young ones interested in the field. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: Baldridge, Lee Ann [mailto:lhad...@iupui.edu] Sent: Thursday, August 07, 2014 3:56 PM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 30years tomorrow. Lee Ann Baldridge IUSM -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4744 / Virus Database: 3986/7994 - Release Date: 08/06/14 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Are Paraffin Blocks Biohazard
Same for IATA (shipping )We have to take the training every 3 years... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, August 08, 2014 2:00 PM To: Dawn Bugge; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Are Paraffin Blocks Biohazard Dawn I think it may be on the OSHA website under the bloodborne pathogen standard - fixed tissue is considered non-infectious and non-hazardous. Below is what OSHA considers potentially infectious materials and specifically addressed unfixed tissue, fixed tissue would therefore be considered non-infectious. occupational exposure to blood or other potentially infectious materials (OPIM) place workers at risk for infection with bloodborne pathogens. OSHA defines blood to mean human blood, human blood components, and products made from human blood. Other potentially infectious materials (OPIM) means: (1) The following human body fluids: semen, vaginal secretions, cerebrospinal fluid, synovial fluid, pleural fluid, pericardial fluid, peritoneal fluid, amniotic fluid, saliva in dental procedures, any body fluid that is visibly contaminated with blood, and all body fluids in situations where it is difficult or impossible to differentiate between body fluids; (2) Any unfixed tissue or organ (other than intact skin) from a human (living or dead); and (3) HIV-containing cell or tissue cultures, organ cultures, and HIV- or HBV-containing culture medium or other solutions; and blood, organs, or other tissues from experimental animals infected with HIV or HBV. The following references aid in recognizing workplace hazards associated with bloodborne pathogens. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Friday, August 08, 2014 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Paraffin Blocks Biohazard Hello Histo World! Our pathologist for our private GI lab would like me to find out if anyone has done a study to determine if the paraffin blocks, once they have been processed, are considered biohazard. I have searched high and low and can find many people stating that the blocks are not bioharzard, with the exception of neurological tissue, but they don't state how they know this. He would like me to reference an actual study to prove that someone has actually looked into this. Any one know of something like this? I know common sense would say that once the tissues have been in formalin for hours, than on the processor for hours that the tissue would be non biohazard and completely safe. Thanks for your help :) -- Dawn R Bugge HT(ASCP), Lab Manager Seattle Histology Dawns Usborne Books Website http://x3128.myubam.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
29. I feel so young... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Thursday, August 07, 2014 1:51 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 44 years here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Peggy Wenk
This is the announcement on the NSH page: http://nsh.org/content/peggy-wenk-true-mentor She will be missed! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of David Kemler Sent: Tuesday, July 29, 2014 7:08 AM To: Fellow HistoNetters Subject: [Histonet] Peggy Wenk Peggy's last interview on HistoTalk www.histotalk.com was taped last year at the NSH. During her interview, I stopped taping several times, because the tears and my choking up were too much for me. Each time I stopped, Peggy smiled at me and said 'It's Ok, Dave, it's OK. It's a shame HistoTalk never got the recognition it truly deserved. Interviews like Peggy's have meaning for everyone in the profession. Over the years, HistoTalk has had a small group of champions - Peggy was one of them! God bless. To Mr. Peggy Wenk, May the Universal Power be within you and your loved ones, now and always. Dave ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histology as art!
Love it! There is a tech that makes jewelry that sells at NSH. Made in a variety of special stains... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Friday, June 27, 2014 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology as art! Hello Histonetters, I'm really looking forward to going to the brand new Morbid Anatomy Museum in NYC, but imagine my surprise when I found some histology in their online gift shop!! http://morbidanatomy.bigcartel.com/category/gifts Histology is beautiful, but it is odd to look at those images on clothing. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: rolling sections
True. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, June 12, 2014 9:58 AM To: Helen Fedor; 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections We do the same thing on our lab. It isn't necessary for them to rollwe just catch them and fold them up and put them in the tube. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, June 12, 2014 10:55 AM To: 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP at NU
Oh yay! CAP is visiting us today. Why do they send an MT to do histo? And a snarky one at that.. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HT HistoDeck question...
We fix HE's in 95% and our IHC protocol is acetone/alcohol fixation. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Watson, Linda Sent: Thursday, October 03, 2013 8:40 AM To: Lee Peggy Wenk; Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT HistoDeck question... For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, HE, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, October 03, 2013 8:16 AM To: Stephenson, Sheryl; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HT HistoDeck question... Personally, I think it's a is a wrong answer, and that you are correct that b is a better answer. My students and I have found a couple of other questions that we thought had the wrong answer indicated in the study set. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Stephenson, Sheryl Sent: Thursday, October 03, 2013 7:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT HistoDeck question... Hi, Please clarify why this answer to the HistoDeck study question is a) and not b). Here is the question: 'Frozen section slides cut from fresh, unfixed tissue specimens are optimally fixed in which of the following solutions? a) 37%-40% formaldehyde b) Cold acetone c) Acetic acid alcohol d) Alcoholic formalin Thanks, Sheryl Stephenson | Histology Technician ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Shipping Slides
Same here and then in a box with more bubble wrap or we use some of those disposable bench underpads for space filler. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Benjamin Sent: Wednesday, July 31, 2013 10:18 AM To: Debbie Granato Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Shipping Slides We use a rectangular cut bubble wrap inside the box on top of the slides, then wrap the entire box in bubble wrap a few times around. Its the best skill to teach your interns, cutting bubble wrap, boxing it up and bringing to fedex, then picking up coffee on the way back! Some slide boxes come with bubble wrap from the vendor, we save those and use when shipping also. Sent from my iPhone On Jul 31, 2013, at 11:04 AM, Debbie Granato debgran...@yahoo.com wrote: Good Morning! Can anyone tell me the best way that you have found to ship slides by Fed Ex? I need to send several cases out and want the safest way possible to eliminate broken slides. We have tried plastic slide boxes with gauze for cushioning and then taped shut and a few other ways. Are there special transport slide containers, other than the 5 slide holders. Any suggestions would be greatly appreciated! Thank you, Debbie Granato HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Competency for Anatomic and Clinical Pathology
We do the blinded sample part. Our manager has us look at an HE stained slide and we identify the tissue (basic histo) and we are asked what stain is appropriate for the tissue (special stain or IHC) Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, July 18, 2013 5:14 AM To: Michelle Lamphere; histonet@lists.utsouthwestern.edu Cc: Elma Cortinas Subject: Re: [Histonet] RE: Competency for Anatomic and Clinical Pathology On Wednesday (yesterday), there was a CAP teleconference on the to-be-updated (to be posted end of July 2013) AP checklist. Someone asked a question about this, mentioning that someone from CAP had said competency assessment does not apply to histology. The reply from the presenter and the CAP office people who could also respond was that competency assess does apply to histology, but that some of the 6 components that have to be checked for may not apply for some or most of the histotech jobs. So if a component of competency doesn't apply, then it doesn't have to be evaluated. Below is part of the standard: GEN.55500 Competency Assessment Phase II The competency of each person to perform his/her assigned duties is assessed. NOTE: The competency of each person to perform the duties assigned must be assessed following training before the person performs patient testing.Thereafter, during the first year of an individual's duties, competency must be assessed at least semiannually. After an individual has performed his/her duties for one year, competency must be assessed annually. Retraining and reassessment of employee competency must occur when problems are identified with employee performance. Elements of competency assessment include but are not limited to: 1. Direct observations of routine patient test performance, including, as applicable, patient identification and preparation; and specimen collection, handling, processing and testing 2. Monitoring the recording and reporting of test results, including, as applicable, reporting critical results 3. Review of intermediate test results or worksheets, quality control records, proficiency testing results, and preventive maintenance records 4. Direct observation of performance of instrument maintenance and function checks 5. Assessment of test performance through testing previously analyzed specimens, internal blind testing samples or external proficiency testing samples; and 6. Evaluation of problem-solving skills These are the 6 components, all of which must be assessed for every task done by histotech - except if it doesn't apply. So, for example, if you are assessing the competency of sectioning, then #2 - reporting of critical values, and #5 - blind testing samples - doesn't apply, so you don't need to assess via #2 and #5. But you would have to assess the person microtoming via the other 4 types of assessment. (But if you are participating in HistoQIP, microtomy is being assess via external proficiency testing sample, so if some of your people's slides were evaluated, you actually have #5 covered for microtomy). So every aspect of the histotech's job (and the lab assistant) must be assessed by as many of the 6 elements above as apply to each task. CAP says we must assess competency, Joint Commission says we must assess competency, and CLIA says we must assess competency, and the 6 elements come from CLIA. So we must assess competency. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on my hospital. -Original Message- From: Michelle Lamphere Sent: Wednesday, July 17, 2013 9:10 AM To: 'histonet@lists.utsouthwestern.edu' Cc: Elma Cortinas Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology I think a few people might find this interesting I recently attended a class about Competency Assessments in the lab. The class was given by Ken Byrd (fairly certain that is how you spell his name), a Senior Inspector at CAP. When this particular question came up, I asked him to give examples of how the histology lab was supposed to use the 6 elements to assess competency. He informed the entire class that the competency assessment question with the six elements did not apply to the histology lab because histology did not report test results. It is the one question on the Gen Lab checklist that did not apply to ANP. Kinda shocking, I know. It does not mean that we scrapped our entire competency program, we simply removed some of the six elements. Michelle Lamphere Senior Tech, Histology Anatomic Pathology Children's Medical Center O
[Histonet] RE: Histology Video
In Providence? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, July 18, 2013 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Video Does anyone know of any Histology parody videos out there on YouTube? Just curious! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Isopentane storage-the polling is open
Always in an explosion proof refrigerator for storage. The isopentane in use is in an isopentane tank (at -70C) in the frozen section area of the gross room as is the fridge Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky Sent: Tuesday, April 16, 2013 2:17 PM To: 'Paula Sicurello'; HistoNet Subject: RE: [Histonet] Isopentane storage-the polling is open We store at room temperature in a flammable cabinet. Use it to freeze muscle biopsies. The Isopentane is pre-cooled by immersing a container of isopentane in a container of Liquid Nitrogen. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 1:35 PM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Primera Printer
From my manager Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu From: Herbert Skip Brown Sent: Tuesday, April 02, 2013 8:27 AM To: histonet-boun...@lists.utsouthwestern.edu Subject: Primera Printer Linda/Jennifer: We have just finished doing a thorough evaluation of the Primera Printer. I agree with Linda that it is a smart new system that prints very well in black white and/or color. We do large clinical trial projects that often require 100+ slide sectioning. The Primera Printer allows you to very easily go in and edit single slides out of a run in case data changes for one slide or there was a typo error by the user. Also and more importantly, the color feature is a tremendous asset to our Q.A. by being able to color code priority cases, individual hospitals or departments, and/or individual microtomists. From the use of color we can now reduce our slide cost by not having to buy various colored slides, and only buying the Epic white slides and print in color. The training was very hands-on via interactive on-line training through their technical support. We are just initiating the unit into our system. Herbert Skip Brown, M.Div., HT(ASCP) Lab Manager - Pathology Core Facility Robert H. Lurie Comprehensive Cancer Center Northwestern University 710 N. Fairbanks Olson 8424 Chicago, Il 60611 (312) 503-3976 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin)
We only babysit when we receive outside slides or the label was not removed prior to staining. It can be adjusted to fit the parameters of the slides used. We have the XL and connected coverslipper. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Thursday, March 21, 2013 1:49 PM To: 'Sullivan, Beatrice'; Bitting, Angela K.; joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin) I love it. For a glass coverslipper. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Thursday, March 21, 2013 8:18 AM To: Bitting, Angela K.; joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin) I'd like to use something a little stronger to describe how much I dislike this coverslipper. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Thursday, March 21, 2013 8:04 AM To: joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin) We hate Leica's CV5030 coverslipper too.!!! Babysit is a good word to describe the user experience. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, March 20, 2013 7:32 PM To: Contact HistoCare; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin) I agree. The Leica stainer and Sakura are both good instruments, however I really HATE that Leica glass cover slipper and I had the same assessment as to need to baby sit. I sometimes preferred just hand cover slipping because it was less trouble, and even faster sometimes ( believe that or not). I had none of these issues with the Prisma covcr slipper, and no trouble with the user interface etc. I had the same issue with the door latch on the Prisma, but other than a quick replacement of that, it worked perfectly. Joelle Weaver MAOM, HTL (ASCP) QIHC From: cont...@histocare.com Date: Wed, 20 Mar 2013 17:49:22 -0500 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin) Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. HistoCare.com Hi, We are currently looking to switch out our linear MKII stainer for either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? Are quantity of HEs is increasing and we need adequate equipment to meet our workload. The incorporated oven seems excellent on both stainers. Any pros/cons would be greatly appreciated! Also, if you are currently using the stainer, does it meet your workload and what is your volume? Thanks! Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Pregnancy
We don't even let them near the stainers, processors or coverslippers. Those I've seen that are expecting can't deal with the smell anyway. It's a teratogen. I wouldn't risk it either. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 05, 2013 12:34 PM To: JStaruk; 'Parker, Helayne'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pregnancy Protecting the employer for a possible liability (and law suit after the fact) cannot cover the compensation for a miscarriage or a permanently disabled child. The issue is not protecting the employer, but protecting the employee and her child. I am sorry, but that is how I see it! René J. From: JStaruk jsta...@masshistology.com To: 'Parker, Helayne' hpar...@skaggs.net; histonet@lists.utsouthwestern.edu Sent: Tuesday, March 5, 2013 1:27 PM Subject: RE: [Histonet] Pregnancy We have a form for all pregnant women (and her doctor) to sign. The form lists the chemicals she will be exposed to and the potential risks of each are outlined. The form also reminds the employee that she should continue to use universal precautions when working around these chemicals. I like to have her doctor acknowledge that he/she is aware of the employee's work environment and that the employee has her doctor's OK to continue working here. I feel that having both sign this form protects us from any possible liability. ___ James E. Staruk HT(ASCP) http://www.masshistology.com/ http://www.nehorselabs.com/ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Tuesday, March 05, 2013 1:10 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pregnancy Hi Gang, Thought I would ask some people with some more experience than I in this. One of our techs is pregnant- Yay !!! She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals. So we need to ask what sort of precautions need to take place during the pregnancy etc. We also coverslip by hand here out of xylene etc. I can not remember ever working with any pregnant HTs in the past so I honestly do not know. Please carbon copy replies to jascholefi...@skaggs.net as well. We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos) Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hpar...@skaggs.net Web: www.coxhealth.com/branson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: QC Slide retention
We keep ours for 2 years- kind of like those lovely QC sheets for CAP. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Monday, March 04, 2013 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QC Slide retention How long do you folks retain you Daily QC slides? Is it the same as the diagnostic slides? I know the correct length of time for diagnostic slides in my particular area, but I wasn't sure if the same timeline applies to the actual daily HE or PAP QC slides. Is the retention of the actual documentation that I have of the quality of these stains each day sufficient? I looked thru the archives, but could only find the previous discussions on Diagnostic slides. Thanks Histonetters!! Rebecca Riesen, Histology Supervisor, PRMC, Naples, FL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: slides and blocks discards
Correct- we had to learn all this for IATA training (how to package and properly ship biological samples). There's a massive list. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Monday, February 18, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides and blocks discards I found this on the CDC website: http://www.cdc.gov/od/eaipp/faq.htm What type of material does NOT require an Etiologic Agent Import Permit? Non-infectious materials - e.g., formalin-fixed specimens, tissues, or slides, or non-infectious human stem cells or non-infectious human organs for transplantation. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.commailto:l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Microtome blades
VWR Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, January 09, 2013 9:56 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Microtome blades Does anyone know who I can purchase Accu-edge blades from, other than Cardinal or American Master Tech? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] 88305TC starting to hit the fan...
But can they afford to buy it Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, November 20, 2012 7:58 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] 88305TC starting to hit the fan... Tell that to all those who have pre-existing medical conditions that can now buy health insurance. René J. From: David Kemler histot...@yahoo.com To: Fellow HistoNetters Histonet@Lists.UTSouthwestern.edu Sent: Monday, November 19, 2012 9:49 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... Hmmm...I think more people should have paid attention to Obamacare two years ago when it was being shoved down eveyones throat. Oh well.. as old saying goes...You ain't seen nottin' yet! I'm just amazed that there are so many in the profession who are surprised about the changes beginning to take place, there are many, many more to come in 2013 14 and all of them affect your job or lack thereof. :) Everyone had the opportunity to change things on November 6th - they chose not to. So, you live with it. Yours, David From: Davide Costanzo pathloc...@gmail.com To: Webster, Thomas S. twebs...@crh.org Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Monday, November 19, 2012 6:46 PM Subject: Re: [Histonet] 88305TC starting to hit the fan... While this stinks on many levels, I have to take issue with the shift wealth from specialists to family practice - family practice docs have been the frontline of medicine, all the while earning less than a quarter of what specialists earn. It's about time they get a boost. Too many specialists earn over a million a year, while the family practice guys/ladies can barely pay their student loans. Sent from my iPhone On Nov 19, 2012, at 1:25 PM, Webster, Thomas S. twebs...@crh.org wrote: CAP had a webinar last week about the cut. These are some very scary times. For some reason the government has decided to shift wealth from specialists to family practice. I am becoming more angry with the affordable care act everyday. http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverrid e=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtl t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar s.html_state=maximized_pageLabel=cntvwr CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Devasting news on 88305TC component
Bear in mind it only 88305. 't's not the only CPT code we use for billing. Just all those biopsies..Yes, that will mess up those independent labs that just do biopsies. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Monday, November 05, 2012 10:11 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Devasting news on 88305TC component It is terrible for anyone that works in an AP lab. There will be job loss from this and some labs will close their doors. There is a lot of blame for this to go around. I blame client billing the most. The government is tired of being the pull through business for labs that are doing the TC so low. Why should the government pay so much when some labs are doing the TC for peanuts in these client billing schemes? I am sure that played a huge role in why they made such a drastic cut. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: disposal of formalin
Our department of Research Safety picks it up (5 gal) container. They are responsible for disposal. I believe it goes offsite. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie Sent: Friday, August 17, 2012 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] disposal of formalin Would anyone be willing to share how formalin is disposed of at your facility? Neutralization, disposal off site, etc. Thank you. Debra Lake MT(ASCP) Manager Micro, Blood Bank, Pathology Marion General Hospital Marion, IN 46952 (765) 660-6521 Fax: (765-651-7330) If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin
We add our own 1% phloxine in 95% alcohol. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Thursday, August 09, 2012 10:28 AM To: Hannen, Valerie; histonet Subject: [Histonet] RE: Eosin Buy one with Phloxine in it. It will make it more red and stronger... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, August 09, 2012 10:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the HE's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin 2) making sure that the alcohols after Eosin are the proper concentrations3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com * = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: counterstain for Alcian Blue (ph2.5)
As does Rowley Biochemical. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Wednesday, August 01, 2012 11:55 AM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: counterstain for Alcian Blue (ph2.5) If I recall correctly Newcomer Supply and Poly Scientific both sell NFR. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig [dmcc...@ckha.on.ca] Sent: Wednesday, August 01, 2012 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] automated H+E and cover slipper
I second this!!! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Wednesday, July 18, 2012 3:44 PM To: Joe Hardin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] automated H+E and cover slipper Leica Autostainer XL with CV5030 coverslipper and transfer station. This has been a real timesaver for us. It automatically moves the slides from the stainer to the coverslipper. It has been relatively trouble free. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Hardin Sent: Wednesday, July 18, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automated H+E and cover slipper Hi All, I will be trying out new H+E autostainers and cover slippers soon. Does anyone have a favorite, and why? Thanks for your responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Beecher
All ,I know this came up before,but who took over Beecher? We need to order some more punchers. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] A poem
I've had this for years- don't know where I got it but thought you all might be amused. LAB HIERARCHY Chief Pathologist Leaps tall building in a single bound Is more powerful than a locomotive Is faster than a speeding bullet Walks on water, Gives policy to God Associate Pathologist Leaps short buildings in single bound Is more powerful than a switch engine Is just as fast as a speeding bullet Walks on water if sea is calm Talks with God The Department Head Leaps short buildings with a running start and favorable winds Is almost as powerful as a switch engine Is faster than a speeding bullet Walks on water in an indoor pool Talks with God if special request is approved Director of Laboratories Rarely clears a Quonset hut Loses tug of war with locomotive Can fire a speeding bullet Swims well Is occasionally addressed by God Associate Director of Laboratories Makes high marks on the walls when trying to leap tall buildings Is run over by locomotives Can sometimes handle a gun without inflicting self-injury Dog paddles Talks to animals Supervisor Runs into building Recognizes locomotives two out of three times Is not issued ammunition Can stay afloat with a life jacket Talks to walls Chief Technologist Falls over doorstep when trying to enter building Says look at the choo-choo Wets themselves with a water pistol Plays in mud puddles Mumbles to Themselves Histotechnologist Lifts tall buildings and walks under them Kicks locomotives off the tracks Catches speeding bullets in their teeth and eats them Freezes water with a single glance They are God - Anonymous Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Friday funny...block alignment
Haven't had that checked lately. How about you? I know my mom may have had something to say to that effect back in high school.. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Friday, June 01, 2012 8:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday funny...block alignment Speaking of block alignment, when this topic comes up, someone invariably pipes up... Hey, is your head on straight? Eric ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CAP
We got an A+ rating. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: Jesus Ellin [mailto:jel...@yumaregional.org] Sent: Wednesday, May 30, 2012 9:34 AM To: Bernice Frederick; Fellow HistoNetters Subject: RE: CAP Have fun and good luck -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, May 30, 2012 7:16 AM To: Fellow HistoNetters Subject: [Histonet] CAP They're here! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] BLock alignment tool
As does TechOne Biomedical. www.techoneweb.com. We have one for each microtome. Just bear in mind if your floor or workstation is not level, it may skew the leveling of the microtome. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Thursday, May 31, 2012 1:57 PM To: Vickroy, Jim Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] BLock alignment tool Newcomer supply has one that works in all standard microtomes. It is rather intuitive. It is essentially a level on an apparatus that goes inside of the block clamp part of the microtome. They call it the universal microtome alignment tool. On Thu, May 31, 2012 at 11:48 AM, Vickroy, Jim vickroy@mhsil.comwrote: Awhile back we got an advertisement for a block alignment tool that would be helpful in recut cases. Can anyone share with me companies that have this device and approximate costs? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP
They're here! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Unregistered techs
All, My point exactly. It belittles what we do (our education and training) and went through to get certified. As I voiced to a an MD the other day, it would be like him calling himself an MD without being licensed. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dorothy Ragland-Glass Sent: Tuesday, May 29, 2012 12:15 AM To: David Kemler; Fellow HistoNetters Subject: Re: [Histonet] Unregistered techs Dorothy R. Glass, BS,HTL(ASCP),IHC You are so right about the good old days when you would prove who you were and sit for a paper not computer exam at a medical school close to you. I sat for the HTL in 1988. I was so proud to call myself a Histologist after being trained at a School of Histotechnology and being ASCP certified. NOW you have people on the job trained just referring to themselves casually as histotects. Not histo trainees. Unfortunate for us older techs, it is very offensive considering what we went through in establishing a career not just a job. Some reference labs is focusing on hiring aids to later, after a few months train them and refer to them as histotechs. I hate it when the term is used so freely. Sinserely, frustrated HTL David Kemler histot...@yahoo.com wrote: Good stuff. In the old days, 36 years ago for me, taking the HT(ASCP) exam it was said that you were registered by the ASCP, because the designation HT is given by the Board of Registry of the ASCP. Many of the NEW folks use the word certified. After a total of 39 years (3 years was training before you were eligible) I still only use registered by the ASCP / Licensed by the State of Florida and call myself a histologist. In those days ( long before the Internet), you took your ASCP exam (HT's, MT's, CT's, BT's, MLT's) at specially selected medical schools across the US. You chose the one giving the exam which was closest to where you lived. If you needed to drive 100 miles or further to get to the examining college on March 15 OR August 15th, (the only dates it was given) that's what you did. Getting into the exam auditorium before you were allowed to sit (that's what it was called) for the exam, was a challenge. You had to prove that you were who you said you were or you were not getting in. Once those guarded doors were closed - they were CLOSED! I saw several folks crying outside the auditorium that day n 1975. Chances for cheating were eliminated at every turn. Unfortunately, not so today. So you can see why for us older techs, if you were HT(ASCP) it really meant something. Unfortunately, not so today. Yours, Dave Histonetters, I see this subject tends to illicit strong sentiments from professionals who are impacted or have an impact on HT/HTL's (sort of everyone on the net)?. I am still in school, but I want to fully understand how training, certification, and registration work for HT/HTL’s. I realize that ASCP certification is voluntary, and that some States require some sort of license or certification, but I’ve never heard of a “Registry” for HT/HTL’s. -The way I understand through what I’ve been taught at school is that Histology is the study of tissue, And that... -To study tissue there is another science that prepares specimens so they can be studied. And that... -There is a final sequence “Quality Control” that verifies the science that prpares specimens is properly done so the tissue can be studied. And that… In order for this all to happen successfully and consistently, the HT/HTL's make sure that during the whole preparation process, safety is observed, proper adherence to federal and state regulations maintained, plus train other technicians to do the same, and much more. If I understood it all correctly I can’t help but wonder: If HT/HTL's do all of this crucial preparation work to make sure specimens are acceptable for precise microscopic identification of cells, tissue type, diagnosis of disease, and other needs: Why wouldn't we want to have some method that can gage a set of basic skills to indicate a level of competency that HT/HTL's should initially have, in order to enter the field of work that can effect so many people either directly or indirectly? And also... Wouldn’t having NAACLS accredited training and ASCP Certifications serve to gage those basic skills? And also… Would gaging basic skills have a positive impact on the quality of patient care and the efficiency of the HT/HTL’s impact on labs?” I'm not sure but...this fall… when I complete my NAACLS accredited degree program, and voluntarily take the ASCP HTL certification exam, my future employer will be able to expect a certain level
RE: [Histonet] Unregistered techs
Than you Jackie. That was the point I was trying to make. I like my job and I hate it when researchers think anyone can do histology, yet even though the MD has to have us tell him or his Post-docs what they need or do not need. Bernce Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor Sent: Thursday, May 24, 2012 8:09 PM To: cha...@yahoo.com; pathloc...@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered techs I've personally tried to train monkeys to do this - they suck at it. I've trained a lot of histotechs, and learned early on that not just any Joe Schmo can do this job (my apologies to any real Joe Schmo's out there). There is a certain skill level and intelligence needed to perform good microtomy, optimize and antibody, or troubleshoot a special stain. I've been in labs where people were just told 'this is a block, put it in the holder on that machine, crank the handle as fast as you can, and pick up what comes off. LITERALLY. This is a skill, and it requires talent. To be good at it requires intelligence and good training. To be great at it requires desire. You're really lucky if you love your job, and I do love this work.I can clicker train monkeys and dogs, but not histotechs. Jackie O' -Original Message- From: William Chappell cha...@yahoo.com To: Davide Costanzo pathloc...@gmail.com Cc: histonet histonet@lists.utsouthwestern.edu Sent: Thu, May 24, 2012 6:02 pm Subject: Re: [Histonet] Unregistered techs I have respected Jay's input in the past, but I too must say something. Without realizing it, and by stating his opinion in a horribly crass way, Jay has touched upon an important truism. There are two types of histologists, those that have a job that pays the bills, and those who have a career in which they thrive. Neither are better than the other, both are needed. I suspect, however, that the majority of Histonetters -- especially avid contributors are in the latter group. I know I am. Histotechs who approach histology as a job, go into work, embed, cut, stain and go home. they are excellent techs, but are just not committed to expanding the field or doing more than is needed to provide the pathologist with a perfect slide. Jay refers to these people as no better than trained monkeys. That is a horrible insult with a small (very small) grain of truth. One day those histologists will be replaced by a mechanical/robotic process. The march of progress is unstoppable. The career histologist has a much longer life span however. We analyze and troubleshoot problems. We understand or endeavor to learn the organic chemistry of stains. We know EXACTLY how a Rabbit Monoclonal antibody is made. We know more about the practice of histology than ANY pathologist. We invent and develop antibodies and special stains. And we conceptualize and perfect the instruments that will replace the first group in the future. Jay, that is why so many are offended. We don't do this simply because it is a good paycheck. We are histologists because we are professionals who choose this career. You may be going to a job cutting slides (which is great and necessary), but we are enjoying our life. Will Chappell, HTL (ASCP), QIHC, MBA and histologist by choice, not accident On May 24, 2012, at 6:48 PM, Davide Costanzo wrote: I'm sorry - I cannot let this rest. The comment: we are just as much needed as pathologists, blah, blah, blah... is so upsetting I cannot sit back and listen to that without saying something! Everyone, regardless of their lot in life, is a very worthwhile part of the whole. Let me ask you a question, since you highly undervalue humans that are not MD's - let's say that you are a patient at Hospital X, and you go in to have your toenail removed. Who plays a more important role in your survival - the Podiatrist or the hospital janitor? I would argue that the janitor is more crucial in this instance, for if he/she fails to clean up the MRSA from the last patient you could conceivably die. The doctor solved your fungal problem, but the janitor prevented you from getting a potentially life-threatening infection. Think before you speak like that - everyone involved in your care is critical - and, yes, sometimes the doctor is not the most important person when it comes to keeping you alive and well! On Thu, May 24, 2012 at 2:01 PM, Jay Lundgren jaylundg...@gmail.com wrote: Scott Lyons sln...@yahoo.com Give me a break, HTs and HTLs do not make diagnoses or treat patients. I am a registered HT and a Florida
RE: [Histonet] (no subject) (Not requiring HT Certification)
If OJT is no longer a valid route, then why can someone with a BS in biology and a years experience in an accredited lab be allowed to take the exam? Most of the people falling into said category learn OTJ and at that learn the lab, not all the theory, so to me, OJT is still there since many of these people never went to histo school. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 24, 2012 9:26 AM To: lpw...@sbcglobal.net; tpodawi...@lrgh.org Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) Peggy Thanks so much for posting this !! I see those job descriptions you speak of all the time. They actually contradict themselves within the description or job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable experience, and then have education requirements of HSD or GED. There are a few people I guess that could be grandfathered, but wat is the certification and education they want/require? Many people I have encountered working in the lab truly don't know the certification eligibility requirements now and think that OJT is still open- even as you pointed out the 7 year time elapse. I stopped trying to correct people's misconception on this and just direct people to the BOC/BOR website for the routes. I have no idea if they ever actually do it, but I do my best to get people to the correct information. I agree supervisors or managers should be more informed on this and check before they advise people, but just my opinion. I do think it is misleading to hire people and allow them to think that this alone can lead to their certification at this point if they don't also pursue the education. I have seen MANY people who fell into this situation and then were unable to change jobs if they needed or wanted to. I think only people who have ever been involved with teaching seem to know about NAACLS. Joelle Weaver MAOM, HTL (ASCP) QIHC From: lpw...@sbcglobal.net To: joellewea...@hotmail.com; tpodawi...@lrgh.org CC: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) Date: Thu, 24 May 2012 05:43:32 -0400 I'd like to wade into this discuss with a couple of comments: LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED HISTOTECHS: Yes, I'm still hearing about places like this. When I talk with the supervisors, it's because the lab wants the person doing the histotech job, but they only want to pay them at lab assistant wages. Plus, once they get the people trained as histotechs, the employees can't go elsewhere, because the other labs only want certified histotech, and these people can't get certified as they don't have the associate degree and minimum 12 hours of biology and chemistry combined as required to take the ASCP HT exam. So these people end up having to stay there. (Personally, I think is very unfair to the employees they hire.) LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: Even though the High School route was dropped as of Jan 1, 2005 (over 7 years ago), I still get emails from labs that want to hire one of my students, but their job description says high school diploma. I usually call these places up, and the histology supervisor had no idea the ASCP HT high school route was dropped. Someone should have told them. Even though it was in every NSH in Action for the 5 years previous (that's now over 12 years ago), in some ASCP publications each year for the 5 years previous, and on both the NSH and ASCP webpage for the 5 years previous, well, since they aren't NSH or ASCP members, well, someone still should have contacted them directly and let them know. Sigh. I've had employees call that they were hired after the 2005 deadline, with the job description of high school graduate requirement, and were told they had 2 years to get the experience required, and then they had 1 additional year in which to take and pass the HT exam. And when they went to sign up to take the HT exam, they discovered that the HT exam requirements had dropped the high school route and now the on-the-job (OJT) requires the associate degree/60 credit hours with 12 credits of bio/chem, which of course they don't have. They tell me that their histology supervisor says they are going to fire them, because they can't take the ASCP HT Exam. I end up talking with the supervisor, and advise them to talk with their HR and Legal departments, as they are the ones who advertised the high school requirement, and they are the ones
RE: [Histonet] (no subject) (Not requiring HT Certification)
So why was OJT supposedly off the charts in 2005 (so to speak). Guess not. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: William [mailto:cha...@yahoo.com] Sent: Thursday, May 24, 2012 9:50 AM To: Bernice Frederick Cc: joelle weaver; lpw...@sbcglobal.net; tpodawi...@lrgh.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) OJT is only available to HTL's via the route you described. Sent from my iPhone On May 24, 2012, at 10:45 AM, Bernice Frederick b-freder...@northwestern.edu wrote: If OJT is no longer a valid route, then why can someone with a BS in biology and a years experience in an accredited lab be allowed to take the exam? Most of the people falling into said category learn OTJ and at that learn the lab, not all the theory, so to me, OJT is still there since many of these people never went to histo school. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 24, 2012 9:26 AM To: lpw...@sbcglobal.net; tpodawi...@lrgh.org Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) (Not requiring HT Certification) Peggy Thanks so much for posting this !! I see those job descriptions you speak of all the time. They actually contradict themselves within the description or job posting itself. Such as ask for HT/HTL certification OR 1 year acceptable experience, and then have education requirements of HSD or GED. There are a few people I guess that could be grandfathered, but wat is the certification and education they want/require? Many people I have encountered working in the lab truly don't know the certification eligibility requirements now and think that OJT is still open- even as you pointed out the 7 year time elapse. I stopped trying to correct people's misconception on this and just direct people to the BOC/BOR website for the routes. I have no idea if they ever actually do it, but I do my best to get people to the correct information. I agree supervisors or managers should be more informed on this and check before they advise people, but just my opinion. I do think it is misleading to hire people and allow them to think that this alone can lead to their certification at this point if they don't also pursue the education. I have seen MANY people who fell into this situation and then were unable to change jobs if they needed or wanted to. I think only people who have ever been involved with teaching seem to know about NAACLS. Joelle Weaver MAOM, HTL (ASCP) QIHC From: lpw...@sbcglobal.net To: joellewea...@hotmail.com; tpodawi...@lrgh.org CC: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) (Not requiring HT Certification) Date: Thu, 24 May 2012 05:43:32 -0400 I'd like to wade into this discuss with a couple of comments: LABS WANTING ONLY HIGH SCHOOL GRADUATES AND/OR NON-CERTIFIED HISTOTECHS: Yes, I'm still hearing about places like this. When I talk with the supervisors, it's because the lab wants the person doing the histotech job, but they only want to pay them at lab assistant wages. Plus, once they get the people trained as histotechs, the employees can't go elsewhere, because the other labs only want certified histotech, and these people can't get certified as they don't have the associate degree and minimum 12 hours of biology and chemistry combined as required to take the ASCP HT exam. So these people end up having to stay there. (Personally, I think is very unfair to the employees they hire.) LABS NOT KNOWING ABOUT THE CHANGES IN HT REQUIREMENTS: Even though the High School route was dropped as of Jan 1, 2005 (over 7 years ago), I still get emails from labs that want to hire one of my students, but their job description says high school diploma. I usually call these places up, and the histology supervisor had no idea the ASCP HT high school route was dropped. Someone should have told them. Even though it was in every NSH in Action for the 5 years previous (that's now over 12 years ago), in some ASCP publications each year for the 5 years previous, and on both the NSH and ASCP webpage for the 5 years previous, well, since they aren't NSH or ASCP members, well, someone still should have contacted them directly and let them know. Sigh. I've had employees call that they were
RE: [Histonet] Histobath
Fisher owns Shandon. Part of Thermo-fisher. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Sunday, April 08, 2012 2:16 PM To: 'Sherwood, Margaret'; marilyn.a.we...@kp.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histobath Is Shandon still around, I never see them anymore? Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Friday, April 06, 2012 12:52 PM To: 'marilyn.a.we...@kp.org'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histobath I googled Histobath and Shandon sells them, plus some other on-line companies. Check it out. Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of marilyn.a.we...@kp.org Sent: Friday, April 06, 2012 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histobath We are desperately looking for a Histobath. I know they do not make them anymore but if someone has a old one they are not using or a company can get their hands on one, we would be eternally grateful. Our Lab Manager would prefer we do not us Liquid Nitrogen. We love the Histobaths we have now. Marilyn Weiss HT (ASCP) cm Kaiser Permanente Hospital San Diego, Ca marilyn.a.we...@kp.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Aetna requiring CAP accreditation for non-hospital labs
I know many certified techs in independent labs and I know for a fact that at least two of them are CAP certified. I can see where an insurance company would want a lab that is doing work that will determine a patient treatment to be on the up and up. I work in cancer research and as the lab for ECOG (Eastern Cooperative Oncology Group)of which many of you submit blocks to for patients on clinical trials. We are in our CAP window right now (so the bosses are stressed). We are all registered techs. And our work can determine chemo arm, future treatments (archival blocks are used for new therapies). Don't mess with us techs not in a hospital. I did it for 20 years. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Monday, April 09, 2012 7:29 AM To: Davide Costanzo; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Aetna requiring CAP accreditation for non-hospital labs Wow David, I strongly disagree with you. I happen to work and run at Dermatopathology lab and I am a licensed Histotechnologist. I have worked in the field for twelve years and I produce the same quality of work that I would if I was in a hospital. I also have to comply with the same state and CLIA rules you do. If not we woundlt be treading on thim ice we would be closed down. CLIA would not let my facility remain open if I was not producing the quality of work expected from all histology laboratories. Also, I cannt believe you would want our labs closed down. Do you know how many of YOUR FELLOW histologist you would put out of work. David is such a team player In reguards to stark laws. Maybe you should read what it actually means We only process specimens within our our facility and our slides ARE read by a licensed dermatopathologist. So, please tell me how my work is any less important than ur hospital job? And let me tell you this about my mediocker job. I work M-F 9-5. no weekends and no on-call. I also have full benefits and 401K. So, Im sorry that you feel our labs are some how underqualified, but I would not go back to hospital work, to save my life. Thanks for having my back, your fellow histologist. Nicole Tatum, HT ASCP This is a fascinating thread!! So what are your thoughts on this - It would appear that, if other insurers follow suit, this could pose a huge burden on dermatologists that do their own tissue processing, and all the GI labs across the country that are popping up doing their own in-house histology. It may be hard, and in some cases not possible for those labs to become CAP accredited. In my opinion, that would be a great thing, to see all those physician offices doing histology close their lab doors, and focus on thier own specialty rather than invade the pathology world from which they were not trained. It would seem, to the average witness, that these facilities are treading on very thin ice as it is. It certainly does appear to be a violation of Stark laws that were created for a very good reason. In addition, these offices have stolen the bread and butter from large labs, and hospital pathology departments and left behind the far less profitable work. It would be nice to see that work return to the place it belongs - in Pathology laboratories. It may be a pipe dream at this point, but who knows - maybe this is the start of a very, very good thing. On Fri, Apr 6, 2012 at 2:56 PM, Kim Donadio one_angel_sec...@yahoo.comwrote: Yikes I just 2 sec ago said that lol Sent from my iPhone On Apr 6, 2012, at 4:51 PM, Jesus Ellin jel...@yumaregional.org wrote: There are several frame of minds here, but most closely this aligns with the affordable care act and quality outcomes for patients. I to agree with the statement that other agencies can provide good quality outcomes, but Anatomic pathology is changing so rapidly. From all aspects, but if you look at who bills for most of the CMS testing it falls under hospital based laboratories, yet the government decides reimbursement based on what the large labs make.. In the end we are seeing consolidation,, but I hope someone comes to the forefront to speak for us all. Sent from my iPad On Apr 6, 2012, at 1:41 PM, Carol Torrence ctorre...@kmcpa.com wrote: I too have been through many CAP inspections in the past. Passing is not my concern - how about expense, prep time, time away to inspect a peer. We are a small private lab also so this a bit of a pain. There is no way that CAP will be able to accommodate the workload that will ensue if this becomes a trend. Which I think
RE: [Histonet] Retention Standards
You can find the CAP retention policies on their web site http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsletter.html_state=maximized_pageLabel=cntvwr Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Thursday, March 22, 2012 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retention Standards Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines? I want to update my retention policy, but I want to ensure that I am using the strictest of the guidelines. I am looking for guidelines on retention of service worksheets, specimen worksheets, requisitions etc and all things paper as well. THANKS for any help and direction you can provide, Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water Quality
Our DI tanks are provided and serviced by Seimens. They do the testing. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 21, 2012 9:35 AM To: Histonet post; lau...@blufrogpath.com Subject: Re: [Histonet] Water Quality When you introduce the words reagent quality it means that a chemical manufacturer has done specific tests to assure that the water complies with such designation. Deionized water is usable for solutions preparations but the issue resides in the deionizer that has to be tested as to its performance. No small laboratory has the conditions required to perform microbiology cultures, nor it is really necessary to do under normal working histology conditions. If these are new CAP requirements it seems to me that CAP is going beyond reasonable requirements. René J. --- On Wed, 3/21/12, lau...@blufrogpath.com lau...@blufrogpath.com wrote: From: lau...@blufrogpath.com lau...@blufrogpath.com Subject: [Histonet] Water Quality To: Histonet post histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 10:27 AM In regards to CAP's Lab General Checklist questions regardin= g water quality: Is deionized water consid= ered CLRW (Clinical Laboratory Reagent Water)? Is water testin= g necessary for a Histology Lab? If so, how do small labs test f= or resisitivity and perform microbiology cultures? Would anyone be willing to share their procedure for Water Quality for a small, private pathology lab? Thank= s, Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processor Preferences??
If it was me and it is in the future, I want a Leica Peloris. Leica also has a new one that is a single chamber version of the Peloris. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Baker Sent: Thursday, March 15, 2012 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor Preferences?? Hello Jennifer, I saw your message about tissue processors on the Histonet Archive May 2006 and wondered which unit you decided to purchase? We are looking for one now and have three in mind, Thermo Pathcentre, Leica ASP300s and the Tissue-Tek VIP6. Your thoughts on your choice and on these listed. Thanks you, John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] saffron vs. safran du gatinais
We buy our alcoholic saffron from Rowley biochemical. Already made and reusable. We were still using it when it hit its expiration date. You can but all the Movat's reagents as a kit or but what you need. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Thursday, January 05, 2012 4:21 PM To: 'Villarreal, Beth'; Histonet Subject: RE: [Histonet] saffron vs. safran du gatinais Beth, In the past, people replied to Histonet with the suggestion to buy saffron aka safran du gatinais from a grocery store spice section. Depending on where you are located e.g. a bigger city, try to find a store that sells spices from India may have the freshest saffron. However, you won't suffer the sticker shock of buying it from a chemical supplier. It still tends to be expensive but not like chemical company prices.Once we made the alcoholic saffron solution for Movat's pentachrome, we stored this solution in a container with a dessicant to maintain a water free environment. I was fascinated that Tom actually grew and harvested saffron from the flowers. That is true devotion, but I suspect it was for those delicious sounding nubbies . Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Villarreal, Beth Sent: Thursday, January 05, 2012 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saffron vs. safran du gatinais Hello histonet, I have a protocol that calls for safran du gatinais and am experiencing some serious sticker shock. Can I substitute saffron in my solution or am I asking for trouble? Many thanks, Beth Beth Villarreal Scientist I Novartis Institutes for BioMedical Research, Inc. 300 Technology Square Cambridge, MA 02139 USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Amazing
We use .5% acid alcohol ( hydrochloric) with Harris hemo. No problems. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, January 05, 2012 10:17 AM To: histonet@lists.utsouthwestern.edu; Sarah Dysart Subject: Re: [Histonet] Amazing You can use either acetic or hydrochloric, both will do. The strength is 1% BUT it does not matter. As a matter of fact the weaker the solution the better. Remember that the acid solution (that can be made with 7ethanolanol even better) is used to differentiate progressivesive hematoxylin, like Harris, and the weaker it is the more control you have with the differentiation. A weak solution allows you to dip the slides several times until you obtain the desired reddish hue on the sections that signals when the differentiation is completed. If the solution is too strong you will have to take the slides often precipitouslyusly and some sections will be have a weak nuclear staining. So, use acetic at 1% in 70% ethanol That is what I used to prepare. René J. --- On Thu, 1/5/12, Sarah Dysart sdys...@mirnarx.com wrote: From: Sarah Dysart sdys...@mirnarx.com Subject: [Histonet] Amazing To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, January 5, 2012, 10:59 AM I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away. That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up). I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well. I want to say it was like a 1% acid solution in alcohol?? What was the acid? For some reason my brain says glacial acetic...but time has made me forget. Is the alcohol you mix it in 100% or something lower with a water content to it? Please help my alzheimers =) Happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control Slides
Same here René, unless we can absolutely not find a positive case. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, December 21, 2011 11:05 AM To: histonet@lists.utsouthwestern.edu; MargaretSherwood Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. René J. --- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Fibulin 3
Or does it detect only 3??? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, November 22, 2011 3:38 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fibulin 3 Does this antibody detect little lies? Sorry, short week and am anticipating the turkey! Claire Subject: [Histonet] Fibulin 3 Does anyone have any experience with Fibulin 3 (EFEMP1) IHC in human tissues? Interested in opinions/observations on staining patterns and sub-cellular localization Thanks in advance and happy thanksgiving to all ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Staffing to work ratios
I wish! Try at least 100blk /day. And that is on an easy day. Can I work with you all? We were taught to cut a block a minute, levels or not. At slowest, one ice tray of 14 blocks in 20 minutes ,with quality. And yes ,I can still do it after all these years. When I started I had 3 months (probation) to get the quality and quantity of techs that had been around for at least 10 years and I was straight out of histo school. Well taught, I might add. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, November 04, 2011 11:16 AM To: Janice Mahoney; stacy_mclaugh...@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios That is only 20 blocks per working day (Mon-Fri)! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, November 04, 2011 11:05 AM To: stacy_mclaugh...@cooley-dickinson.org; histo net Subject: RE: [Histonet] Staffing to work ratios I have always used the 5000 blocks per HT for the year. (not including IHC staff) Jan Mahoney Omaha,NE Date: Thu, 3 Nov 2011 13:03:44 -0400 From: stacy_mclaugh...@cooley-dickinson.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staffing to work ratios Hi, I know this subject has been discussed before, but I'm having trouble finding the info I need from the archives. Would anyone know where I can find the work to staffing ratios for a Histology laboratory? Our volume was ~9750 surgical cases (2010). The majority of them are biopsies (GI, GYN, skin, etc) but we do some larger complex cases. We had 2569 billable IHC tests, 254 billable group I special stains, 722 billable group II special stains. #blocks: 23,380 # HE slides : 49,524 Thank you for your help! Stacy McLaughlin, HT(ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 (413)582-2019 stacy_mclaugh...@cooley-dickinson.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] decal question
Ann Preece states acid decal uses aqueous solutions od either formic,nitric or
trichloroacetic acid. Other methods mentioned are Ion-exchange resin,electrical
ionization and chelation. The histo bible!
Bernice
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, October 03, 2011 11:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decal question
Hi Everyone,
I have a new student taking course work at UND and using my lab for her
practical clinical site. She took a test on decal today and there was a
question we didn't know the answer to.
What fixative should not be used for acid decalcification? Would it be osmium
tetroxide?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email mailto:pru...@ihctech.net pru...@ihctech.net web site
http://www.ihctech.net www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
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dissemination, distribution, copying, or other use of this message, or any of
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RE: [Histonet] Alignment tool
So does TechOne biomedical Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Friday, September 30, 2011 9:45 AM To: jsjurc...@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Alignment tool Newcomer sells one Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Friday, September 30, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alignment tool Who sells the microtome alignment tool that clamps onto the base of the microtome and also into the cassette holder to assure that all microtomes in the lab are in the same cutting plane? Do the bubble ones work as well? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Phosphogard/guard
Has anyone heard of or used this for protection of phosphorylation sites during IHC? My manager wants to try it. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Automatic HE stainer with coverslipper (glass)
Same here. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, September 01, 2011 8:03 AM To: 'Ernestine Middleton'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automatic HE stainer with coverslipper (glass) We have a Leica stainer and coverslipper. We like them both. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ernestine Middleton Sent: Wednesday, August 31, 2011 8:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic HE stainer with coverslipper (glass) Hi; Looking and need comments on those of you that are using combination HE stainer with glass coverslipper. Thank you. Ernestine Middleton, Manager, HT, HTL ,BS ,MPA Montefiore Medical Center Bronx, New York 718-920-4157 emidd...@montefiore.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin
I went through histo school (in 1983-84) and we were taught the skills needed to work in a hospital lab- Many of these came into play when we were preparing our practical. I had to cut at my interview, being a new grad, and once I was hired I had my 3 months probation to have the quality and quantity of the techs already there, most of them had been in the field at least 10 years, so I had a basis and a goal to meet in a short time. I survived and worked there for 15 years and learned how to crank out the work with the needed quality. If trained properly, it should not be a problem. You relearn/adapt your skills to do what is needed in your job- that is the reason I was hired- the hospital wanted a tech easy to retrain to their procedures. Bear in mind, OTJ is no longer a viable route (last I heard) Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Wednesday, August 31, 2011 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin You might be surprised I too agree with Joyce and Richard. I understand very well that a new graduate will not be up to the skill level of an individual that has been working for a while. What I am surprised about is that this program seemed to teach to pass the test and has left all of the technical skills left to be taught by the persons 1st job. This could lead to several painful experiences for not only the facility but the new HT. Were is the disconnect. If a person is doing the OJT route they need to have at least one year of experience signed-off by a pathologist. If a person goes through a program who is responsible for making sure that the base-skills are there? Is there not some standards that a new graduate should be able to cut/embed so many blocks in an hour? Is it reasonable for a new graduate to take 1.5 hours to cut 5 (uterus, appendix, tonsil) one-cuts? From facing to lifting the slide off the water-bath? Or to take 2hrs to embed 15 (large tissue sections) one-cut blocks? So Richard is also right how is a new grad to get experiance without that 1st gig? But how much resposiblity should be placed on the 1st gig to train a new HT that is supose to have base skills in lab equipment, cutting, embeding, staining etal? More thoughts ciao Matt Lunetta BS HT(ASCP) Edwards, Richard E. r...@leicester.ac.uk If you do not employ recently trained individuals, how on earth are they going to obtain the experience that Matt craves, it's beyond me. My daughter recently qualified as an Occupational Therapist, when she asked for the reason that she was unsuccessful in obtaining a post, they said that as a recently graduated student she had no experience, what rubbish!!.Thankfully she has now obtained a position under more enlightened management that is to be found here!. Cheers Richard Edwards Shirley A. Powell 08/30/11 6:32 PM I second that Joyce. sp From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [jwe...@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their Clinicals. They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons
[Histonet] RE: TFE-3
Same here. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Tuesday, August 09, 2011 11:05 AM To: 'Houston, Ronald'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: TFE-3 We use TFE-3 (p16) from Santa Cruz, made in Goat. Cat.# SC-5958 Dana Settembre University Hospital - UMDNJ Newark, NJ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, August 09, 2011 12:02 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] TFE-3 Is anyone using TFE-3? If so I would appreciate which clone is being used most. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual mirotome
I want my AO820 back! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, July 12, 2011 2:40 PM To: sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, sdys...@mirnarx.com wrote: I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual mirotome
I actually use a Microm HM315 and have never had issues with it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Tuesday, July 12, 2011 2:47 PM To: Patrick Laurie Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What is a great manual mirotome We have tried the Leicas thru the years and found that post 2030's that our arms were not long enough to turn the handwheel without completely straightening our elbows. This led to soreness in our elbows. Their microtomes kept getting larger and larger with the handwheel further back. The 2125 solves that problem for us. We really like ours. - Original Message - From: Patrick Laurie foreig...@gmail.com To: sdys...@mirnarx.com Cc: Histonet@lists.utsouthwestern.edu Sent: Tuesday, July 12, 2011 2:40:12 PM Subject: Re: [Histonet] What is a great manual mirotome I've always been a fan of the Leica microtomes, I've used all kinds, from the Leica RM 2025 Microtome to the Leica RM 2265. A good non-automated version is the RM 2235. I've also used the Microm (thermo-fisher-shandon) HM325S which work well. The Sakura Accucut SRM is manufactured by Leica, I believe it is equivalent to the RM2025. I however have never used the Leica 2125. I've always had good service from them, especially if you have a service contract. Good luck! On Tue, Jul 12, 2011 at 12:11 PM, sdys...@mirnarx.com wrote: I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lsc...@sfcn.org Sent: Tuesday, July 12, 2011 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] What is a great manual mirotome Hi, Our small lab is looking for some advise on what microtome to replace a fairly new Leica RM2235 with. We have been looking at the Sakura SRM 200 and the Leica 2125. We are looking for reliability and have net gotten it from the RM2235. Is anyone using either of these that would be willing to offer suggestions? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Retirement
Pina coladas here.!!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, June 17, 2011 11:43 AM To: Cynthia Robinson; Bill O'Donnell; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Retirement I 'm game for that as long as there is Tequila around. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer ___ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson [robin...@mercyhealth.com] Sent: Friday, June 17, 2011 12:13 PM To: Bill O'Donnell; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Retirement Bill, I am about the same age as you are and would like the same type of locale. Since there are so many of us in this field due to retire in the next 15-18 years maybe we should consider investing in a Histotech Retirement Community. Specimens could be shipped to us for processing and staining. We could set up microtome stations in the common area and we could get together and talk and cut at the same time and do it in a wonderful climate with nice views and of courseat our own pace which will be relaxed and more of a shuffle that a full out sprint. Anyone have any suggestions for naming such a paradise? Ok...I'm just getting old and it is Friday..so hope you appreciate my humor. Have a good weekend everyone! From here in the Midwest, where waterfront property is in abundance along the Mighty MO Cindi Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robin...@mercyhealth.com O'Donnell, Bill billodonn...@catholichealth.net 6/17/2011 11:00 AM OK, I know it is Friday, and I know that this may sound like a bit of a jokebut I am 15-18 years out from retirement and my wife and I want to retire someplace tropical And it would be smart to get settled in such a location. So, if anyone knows of any openings in Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT (ASCP) QIHC PLEASE let me know. Would be open to others, but would prefer a US territory. I can be reached at b...@deaconbill.com William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formaldehyde in H2S's 12th RoC
Well we still use Hematoxylin powder, Xylene ,Oil Red O, Beibrich scarlet and Congo red and we haven't been stopped yet. I'd think embalmer would be well-fixed and preserved ,rather than have a high mortality rate. Have the proven it's from formaldehyde? Whom knows what the get from a dead body? Bernice -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, June 13, 2011 9:11 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde in H2S's 12th RoC The US Department of Health and Human Services (sometimes affectionately known as H2S) has released the 12th Report on Carcinogens (RoC). The report lists formaldehyde as a definite human carcinogen. The regulatory repercussions of this aren't immediately clear, but I suspect pathologists will see increasing pressure to eliminate formaldehyde fixation. As the snake oil peddlers come out of the woodwork, it's going to become increasingly important to remember that there is no substitute for formaldehyde that will not greatly change the way pathologists and histologists work. A not entirely satisfactory New York Times account is at: http://www.nytimes.com/2011/06/11/health/11cancer.html?hp The report itself can be obtained in pieces in PDF form at: http://ntp.niehs.nih.gov/?objectid=72016262-BDB7-CEBA-FA60E922B18C2540 The 12th RoC reports repeat the mantra studies show at Joyce Brothers length, but do not really make clear what the facts are. They do offer these references about mortality among embalmers and pathologists. Does anyone on the list have access to these papers? Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2003. Mortality from lymphohematopoietic malignancies among workers in formaldehyde industries. J Natl Cancer Inst 95(21): 1615-1623. Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2004. Mortality from solid cancers among workers in formaldehyde industries. Am J Epidemiol 159(12): 1117-1130. Hauptmann M, Stewart PA, Lubin JH, Beane Freeman LE, Hornung RW, Herrick RF, et al. 2009. Mortality from lymphohematopoietic malignancies and brain cancer among embalmers exposed to formaldehyde. J Natl Cancer Inst 101(24): 1696-1708. Posting this both to PATHO-L and to Histonet. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica CV 5030
Micromount (Surgipath- now a part of Leica). 21 gauge needle. No problem. Make sure volume set is correct for the media. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 09, 2011 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica CV 5030 Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] question on H pylori
We did diif -quik or Alcian Yellow. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Friday, June 10, 2011 9:10 AM To: Setlak, Lisa; histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: RE: [Histonet] question on H pylori We do either IHC or a Modified Steiner, depending on the Pathologist's preference. We switched to Newcomer Supply's Steiner-Chapman Modified Silver Stain Kit about a year ago, because it eliminated the use of Uranyl Nitrate. We have been delighted with the consistency of the stain. Sandy Harrison, HTL (ASCP) Anatomical and Surgical Pathology Supervisor, VAMC Minneapolis 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Tuesday, June 07, 2011 1:12 PM To: histonet@lists.utsouthwestern.edu; 'histonet-requ...@lists.utsouthwestern.edu' Subject: [Histonet] question on H pylori I was just curious what everyone is using for standard of care regarding H = Pylori..is everyone doing IHC or are you doing a Giemsa? Thanks, Lisa Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin blocks from cell line
You can use Histogel as well from Richard Allan (Fisher or VWR) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS Sent: Wednesday, May 18, 2011 4:51 AM To: kst...@mcw.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin blocks from cell line we made a cell block from cell line by following procedure: - centrifuge cell suspension - fix the sediment overnight in formalin (for tissue fixation) - optional, the button will be anyway fixed by subsequent procedure - centrifuge and decant the formalin - add few drops of liquid agar (not too hot) - as the button solidify you can put it in cassette and process as diagnostic tissue. We got good immunoreactions on sections prepared from melanoma cell line for example. hope this helps Irena Kirbis From: kst...@mcw.edu To: histonet@lists.utsouthwestern.edu Date: Tue, 17 May 2011 13:45:41 -0500 Subject: [Histonet] Paraffin blocks from cell line Hi histonetters, Has anyone made up paraffin blocks from cells from a cell line. This is new to me. I assume they will be in some kind of a media. We will be getting the cells and incubating them to grow. Thanks in advance for the help Kathryn Stoll, HT(ASCP) Depatment of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kst...@mcw.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] NSH symposium registration
Try i...@nsh.org. They'll let you and us know. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Denise G Crowley Sent: Thursday, May 05, 2011 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH symposium registration Hi all, Does anyone know if we will be receiving booklets from NSH or if everything is to be done on line? Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencr...@mit.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat repair
We use them too- Illinois office. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bouchal, Rena L Sent: Thursday, March 31, 2011 1:38 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat repair We are using Tech One Biomedical... service reps in Pghfor almost all of our routine instrument service really good and half the price of the vendors! Their number is 866-497-3033 or www.techoneweb.com Please note that my email address as of Jan 3, 2011 is bouch...@wvuhealthcare.com . Please make the appropriate changes in your address book. Rena Bouchal, M.S. Anatomic Pathology Manager West Virginia University Hospitals 304-293-7765 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DANIEL HEWITT Sent: Thursday, March 31, 2011 2:15 PM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat repair Emily You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars and do most of our repairs. Ray does an excellent job at a good price. It sounds like the bearings in your fan or maybe the compressor. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, March 31, 2011 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat repair Our cryostat (CM3050S) is making a terrible noise when the fan stops running, yet I don't want to call Leica (the manufacturer) because they charge more money than anyone could ever afford. How would I go about finding someone who could repair a cryostat in Pittsburgh? I don't think it's the refrigeration that's broken, it sounds like something mechanical. Any suggestions? Has anyone ever tried to open the back of one to see if it's fixable? I'm no mechanic, but I think I can spot a worn belt or loose screw. I'm just not sure I want to open it up myself. Emily It has become almost a cliche to remark that nobody boasts of ignorance of literature, but it is socially acceptable to boast ignorance of science and proudly claim incompetence in mathematics. -Richard Dawkins ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Daylight Savings Time?
I think it used to be the first weekend in April,but I was also told it may have been this coming weekend (in Europe anyway) Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, March 14, 2011 8:00 AM To: Histonet Subject: [Histonet] Daylight Savings Time? Does anyone know the OLD daylight savings time date that USED to be in effect? I'll have to manually change the processor time to avoid being late (or early - who knows?). Today, I was way early! Senior moment, I suppose... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] expired abs
I don't think mine were ever made,let alone expired! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Thursday, March 10, 2011 10:17 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] expired abs I think my abs expired long ago! Happy Friday Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Patsy Ruegg Sent: Thu 3/10/2011 8:28 PM To: 'ihcrg Group (E-mail)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] expired abs Just wanted to remind everyone if you are about to discard expired abs I would be willing to take them and put them to good use. If you have expired abs to get rid of contact me and I will provide you a fedex acct. # so you can send them to me rather than discard. This effort paid off recently because I sent a care package of expired abs and detection reagents to a hospital tech in Ghana West Africa who is using them to test patients there because they have training in ihc but do not have the abs or detection reagents to do it. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette labeling
B Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Wednesday, February 09, 2011 11:15 AM To: 'Lee Mayhew'; Histonet Subject: RE: [Histonet] Cassette labeling A For filing reasons. When we file in the boxes on top of microtome they are right side up and for final filing in the plastic drawers writing is right side up. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Mayhew Sent: Tuesday, February 08, 2011 3:55 PM To: Histonet Subject: [Histonet] Cassette labeling Hi Histonetters, At my hospital, we are having a discussion about how to label cassettes. I have worked at 2 hospitals, and they each do it a different way. Our cassette labeller will print either way. Could you please indicate which way you do it at your site, A or B. A..When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is upside down. B.When the cassette is open and sitting on the bench facing you with the lid on the far side and the surface for writing on is closest to you, the surgical number is right side up. Thanks in advance. Lee Mayhew MLT St. Josephs Hospital Hamilton, ON Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Wear gloves when handling / cutting paraffin blocks.
We wear gloves we have to sterile section blocks for DNA/RNA extraction. Keeps the slides free from contamination if we are placing sections on slides, otherwise we are sticking them in a vial. All equipment associated with the sterile sectioning is cleaned between each block (block holder, blade holder, forceps etc) and gloves are changed. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tahs...@brain.net.pk Sent: Saturday, October 23, 2010 8:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wear gloves when handling / cutting paraffin blocks. Dear All, I am looking some kind of references out there that addresses that it is not a requirement to wear gloves when handling / cutting paraffin blocks. Any help would be greatly appreciated Thank in advance, Muhammad Tahseen Senior Supervisor Histology SKMCHRC Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
