Re: [ccp4bb] Ligand relevance for bioinformaticians using PDB files

Firslty, there is credo http://marid.bioc.cam.ac.uk/credo a database set up in the Blundell lab, that deals with protein ligand interactions. That might be of interest. Second, as more general points. I see your point Shane, I'd argue there is no great value in trying to distinguish artefacts

Re: [ccp4bb] ppGpp purification and concentrate

Can you elute from AE with NH4HCO3? It's volatile, so you could just evaporate it off afterwards. JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of luzuok Sent: Friday, June 12, 2015 7:13 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] ppGpp purification and concentrate

Re: [ccp4bb] ppGpp purification and concentrate

desiccator with P2O5 in the freezer should do the trick... Artem - Cosmic Cats approve of this message On Thu, Jun 11, 2015 at 11:12 PM, luzuok wrote: > > Dear ccp4bb members, > > Sorry for asking a non-crystallographic question where google search > cannot provide any valuable answer. > >

[ccp4bb] ppGpp purification and concentrate

Dear ccp4bb members, Sorry for asking a non-crystallographic question where google search cannot provide any valuable answer. pppGpp was synthesized by relQ enzyme using ATP and GTP, the product was purified by anion exchange chromatography. The problem is that the concentration of pp

[ccp4bb] Post-doc available on membrane protein structure determination at Columbia University

A postdoctoral position is available in the laboratory of Dr. Filippo Mancia in the Department of Physiology and Cellular Biophysics at Columbia University in New York, USA. The labs primary interest is in unraveling the structure and function of eukaryotic and prokaryotic membrane proteins, wit

[ccp4bb] Peltier cooled incubators

Little off topic and after a quick search I could not find much on the BB already. We are looking at purchasing a larger Peltier cooled incubator for crystal trays. They are designed to go down to 15 C. I know people have had pretty good luck with the bench top models but we are looking at a

Re: [ccp4bb] Ligand relevance for bioinformaticians using PDB files

Hmm, this makes me think of how upon deposition to the PDB, we describe the expected biological assembly that a crystal structure represents. This is a judgement call, but can be backed up with experimental data. A similar flag for physiological ligands as opposed to components of the crystallizati

[ccp4bb] Ligand relevance for bioinformaticians using PDB files

This week a bioinformatics PhD candidate was talking about his PDB analysis tool which was aimed at studying protein-ligand statistics and asked for some advice on what should he consider as "biologically relevant" or not. I told him many of the most common molecules he found were buffers, cryoprot

[ccp4bb] PREDOCTORAL POSITION IN STRUCTURAL BIOLOGY (BARCELONA)

PREDOCTORAL POSITION IN STRUCTURAL BIOLOGY Applications are invited for a predoctoral position in the group headed by Prof. Miquel Coll at the Institute for Research in Biomedicine (IRB Barcelona) / Institute of Molecular Biology of Barcelona (IBMB-CSIC) to work on the project “Structure of

[ccp4bb] Postdoctoral Position

A postdoctoral position in structural biology is available in the Shamoo Lab at Rice University (http://www.bioc.rice.edu/~shamoo/shamoolab.html ). The successful candidate must have experience in X-ray crystallography as well as biochemistry. Candidates should hold a PhD degree in biophysics or b

[ccp4bb] Release of iMosflm/Mosflm version 7.2.0

Dear all We are pleased to announce the release of iMosflm/Mosflm 7.2.0; this version includes a number of new features as well as the usual "numerous bug fixes" - • iMosflm now has a "known cell" indexing option that can be useful for difficult cases if the cell parameters are known a

Re: [ccp4bb] OT: looking for a 3D-beamer that works with nvidia nvision

Hi, if you plan to use the IR emitter and work in groups (> 10 people), you are probably going to need a stronger IR emitter. If people are sitting in rows, the IR emitter signal can get lost in the backrows. Better to have a short throw beamer so nobody gets in the way (at a distance of 1 met

[ccp4bb] Instruct Call for proposals

Call for proposals utilising Instruct-funded structural biology techniques If you don't have local access to ALL the structural biology instrumentation you need for your research you can access some of the best structural biology facilities in Europe. Instruct has 16 Centres around Europe and

Re: [ccp4bb] twinned data refinement

If you have unmerged data try running it through pointless/aimless/ctruncate. That analyses twin fractions very carefully and with that twin law you should see some evidence of 6 fold symmetry but less pronounced than for the 3 and 2 fold symmetry operators.. Eleanor On 11 June 2015 at 01:03, Men

[ccp4bb] PhD position in the laboratory AFMB, Marseille

Dear all, A PhD position within the Marie-Curie (ITN) network is available in the laboratory AFMB (Marseille, France) to work on the mRNA capping machinery of Alphaviruses. A detailed description of the topic and eligibility can be found at the following link http://hactar.phrmy.cf.ac.uk/an

[ccp4bb] Diamond MX bag training 15-16th July - with registration link working

Sorry I sent the wrong link for the registration... Here is the new one: Registration open until 24th June Pierre Dear all, The Diamond Light Source Macromolecular Crystallography group would like to invite both our academic a

[ccp4bb] OT: looking for a 3D-beamer that works with nvidia nvision

Dear CCP4ers, sorry for the off-topic mail: We are looking for a 3D-beamer for teaching purposes (structural biology courses). In the "teaching rooms" we have lots of high-end workstations equipped with 3D-stereo (Nvidia Nvision2, Quadro K5000, ASUS 27" VG278HE) that work perfectly in 3D (Scie