there
are numerous possibilities.
Kind regards,
Isabel
On 7 Jul 2017, at 12:57, Dr. Isabel De Moraes
mailto:isabel.de-mor...@diamond.ac.uk>> wrote:
Any suggestions regarding to the positive densities?
The crystallisation condition only has NaCl and CaCl
[cid:DD08D41F-CAB5-458
Any suggestions regarding to the positive densities?
The crystallisation condition only has NaCl and CaCl
[cid:DD08D41F-CAB5-458D-BBD0-5DDCADC746FF@diamond.ac.uk]
[cid:B6BDEE5E-325C-4FEF-A7BC-B4F679D5315F@diamond.ac.uk]
Best regards,
Isabel
--
This e-mail and any attachments may cont
Dear all,
Applications are invited for a full-time Research Technician position at the
Membrane Protein Laboratory (MPL) located at the Diamond Light Source and at
the Research Complex (Oxfordshire).
The MPL is Wellcome Trust funded facility created to allow scientists to
determine medically
Dear all,
Applications are invited for a full-time Research Assistant position at the
Membrane Protein Laboratory (MPL) located at the Diamond Light Source and at
the Research Complex (Oxfordshire).
The MPL is Wellcome Trust funded facility created to allow scientists to
determine medically r
Dear Amit,
You are working with a membrane protein and the use of SDS (harsh detergent)
often makes these proteins to oligomerise. Boiling your sample is not
advisable, it might make it worse.
I’m not sure why you would like to “see” a monomer in the gel but if you really
would like to know
Dear CCP4 colleagues,
The Membrane Protein Laboratory (MPL) at the Diamond Light Source and GE
Healthcare are organising a FREE Symposium on Membrane Protein Sample
Preparation & Analysis.
The symposium will take place on the 10th November, 9:30am at the Pickvance
Lecture Theatre, Harwell
Dear Andre,
It is a small protein then, I recommend to do the purification in a small
micelle detergent (e.g. OG). This regardless the extraction done in DDM or DM
or any other suitable detergent.
You can start by reading
Moraes, Isabel, et al. "Membrane protein structure determination—the ne
oracle/apps/irc/candidateSelfService/webui/VisVacDispPG&akRegionApplicationId=821&transactionid=1538103408&retainAM=Y&addBreadCrumb=S&p_svid=43842&p_spid=1669138&oapc=9&oas=dHtHWqciP0_3C_VtnV-LXQ
Kind regards,
Isabel
----------------
9, 920–923
--------
Dr Isabel De Moraes, MRSC
Membrane Protein Laboratory
Diamond Light Source
On 22 Apr 2014, at 13:44, Campeotto, Ivan wrote:
Dear Yahui,
Your problem is quite common and very frustrating,
------
Dr Isabel De Moraes, MRSC
Membrane Protein Laboratory
Diamond Light Source
On 16 Feb 2014, at 15:58, Raji Edayathumangalam wrote:
> Hi Everyone,
>
> After several attempts to cleave the SUMO tag off my
Dear Stephen,
Many thanks for this post.
The lecture is really great, I would recommended it to all interested in the
field (from beginners to seniors).
Best,
Isabel
Dr Isabel De Moraes, MRSC
Membrane Protein Laboratory
.
Best,
Isabel
------------
Dr Isabel De Moraes, MRSC
Membrane Protein Laboratory
Diamond Light Source Ltd,
Harwell Science and Innovation Campus,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK
--
e last three years.
For informal enquires please contact Dr Isabel Moraes
(i.mor...@imperial.ac.uk<mailto:i.mor...@imperial.ac.uk>)
--------
Dr Isabel De Moraes, MRSC
Head of the Membrane Protein Laboratory
Diamond Light Source Ltd,
Har
n:http://events.embo.org/13-htp-protein/application.html
With best Regards,
Isabel
On 14 Oct 2012, at 13:09, Dr. Isabel De Moraes wrote:
> Apologies for the previous email, almost impossible to read. Hopefully,
> this one looks better.
>
>
> Dear All,
>
> We are happ
Dear All,
I would like to retract this post since the information is wrong.
My sincerely apologies.
I will send the right information shortly.
Isabel
On 9 Jan 2013, at 11:16, Dr. Isabel De Moraes wrote:
> Dear All,
>
> A gentle reminder that the deadline for the practical
2 Dec 2010, at 15:52, Dr. Isabel De Moraes wrote:
>
> Dear Structural Biologists,
>
> The Oxford Protein Production Facility (OPPF-UK) and The Membrane Protein
> Laboratory (MPL) at Diamond are offering a
> "hands-on" course in "High-throughput Protein Productio
Deal All,
Like many others, I also would like to encourage to listen the “In Our Time”
BBC Radio 4 program next week as part of the Bragg Centenary Celebration.
Melvyn will have Prof Mike Glazer (Oxford), Dr Chris Hammond (Leeds) and Prof
Judith Howard (Durham) as guests.
A subject not to be
Apologies for the previous email, almost impossible to read. Hopefully, this
one looks better.
Dear All,
We are happy to announce an EMBO Practical Course in “High throughput Protein
Production and Crystallisation”
The overall aim of the course will be to review the state-of-the-art in HTP
Dear All,
We are happy to announce an EMBO Practical Course in
“High throughput Protein Production and Crystallization”
The overall aim of the course will be to review the state-of-the-art in HTP
structural biology with an emphasis on methods to study complex targets,
including membrane proteins.
tp://www3.imperial.ac.uk/>
We would be delighted to meet you at the symposium.
With best Regards,
Isabel Moraes
-----------------
Dr. Isabel De Moraes, MRSC
Membrane Protein Laboratory Facility Co-ordinator
Membrane
,
Isabel
-
Dr. Isabel De Moraes, MRSC
Membrane Protein Laboratory Facility Co-ordinator
Membrane Protein Laboratory
Diamond Light Source Ltd,
Chilton, Didcot, Oxfordshire,
OX11 ODE, UK
Tel (direct): 01235
Dear Raji,
The best way to find out is to run a SEC-MALLS (Size Exclusion Chomatography -
Multi-Angle Laser Light Scattering) experiment.
Best,
Isabel
-
Dr. Isabel De Moraes, MRSC
Membrane Protein
between plates due to the tip washes).
Regards,
Isabel
-
Dr. Isabel De Moraes, MRSC
Membrane Protein Laboratory Facilities Co-ordinator/Group Leader
Membrane Protein Laboratory,
Diamond Light Source Ltd,
Harwell Science and Innovation Campus
around Diamond you are welcome to have a look.
Regards,
Isabel
-
Dr. Isabel De Moraes, MRSC
Membrane Protein Laboratory Facilities Co-ordinator/Group Leader
Membrane Protein Laboratory,
Diamond Light Source Ltd,
Harwell Science and Innovation Campus
Call for applications to the Membrane Protein Lab at Diamond
Dear Crystallographers,
The Membrane Protein Laboratory (MPL) at Diamond Light Source in
Oxfordshire (UK) is pleased to announce that is now open our call for
proposals.
The MPL is a joint venture between the Diamond Ligh
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