Re: [Histonet] Tissue Embedder

Tec5 Embedding system from Sakura does it for me - ergonomic and left/right handed Annie 2009/9/3 Perry, Margaret > We are in the market for a new embedder. What do you suggest? I tried > searching the archives but only came up with older comments. > Vendors please feel free to contact frank.

[Histonet] Ink issues

We  blot the tissue dry, ink specimen and dip into 50% vinegar .  Black and green work best, but all colors work. Judy McKinney JPS Hospital Fort Worth Texas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mai

[Histonet] Dragon dictating system

Histonet, Has anyone had any experience with the Dragon dictating system? Pro's or Con's? Thanks for your opinions Judy McKinney    John Peter Smith Hospital Fort Worth Texas 817-927-1024 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Tissue Embedder

We are in the market for a new embedder. What do you suggest? I tried searching the archives but only came up with older comments. Vendors please feel free to contact frank@sdstate.edu. We may have to go with a used instrument so would like comments on good

Re: [Histonet] Re: anti-GFP antibody

Hi all, Just to update you, I ordered Abcam's ab13970 chicken anti-GFP and tested it out in paraffin embedded sections with HIER for 10 minutes in Dako target retrieval system. I came in with a Dylight 488 secondary from Jackson Immunoresearch at 1 ug / mL. It worked beautifully at 1:500, 1:1000,

RE: [Histonet] Cost to produce an H&E slide

Over the years I have not found any published cost calculations that have addressed individual categories that fluctuate constantly in the department and it seems that there are as many models as there are institutions/companies. In the past I have found if I separate costs into a minimum of 4

[Histonet] pERK IHC

Does anyone have any suggestions for antibody, protocol, etc., regarding staining FFPE sections with pERK? Any information would be greatly appreciated. Thanks in advance, Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit _

RE: [Histonet] Cost to produce an H&E slide

The cost around $12 to produce a regularly stained section. The cost to produce a stained section by an very experienced histotech...priceless. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of B

RE: [Histonet] Endogenous biotin blocking

Sally: The simple answer to the question of whether or not biotin blocking is required is ‘no’. As you stated in your inquiry, blocking need only be performed when excessive nonspecific staining, attributable to endogenous biotin, is observed. For what its worth, I think that some folks be

RE: [Histonet] Re: Gloves

We also use the Aloetouch gloves for all aspects of Histology and have had no issues with them. They fit "like a glove" and are very durable but continue to have good flexability. Luckily Medline is on contract with HPG (our buying group)!!! Dawn D. Schneider, HT(ASCP) Howard Young Medical Cent

RE: [Histonet] Cost to produce an H&E slide

I don't know what the COST to produce an H&E is but we charge outside entities $8 each to stain them. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Amy Johnson Sent: Wed 9/2/2009 11:

RE: [Histonet] Re: Gloves

I was just doing some research on the original question posted of gloves suitable for handling xylene. Nitrile is a synthetic rubber made of acrylnitrile and butadiene, devoid of allergy-causing proteins like in latex and natural rubber. The U.S. Dept. of Energy suggests using nitrile when ha

[Histonet] (no subject)

Does anyone have a good recipe for making up a dissect aid in house? Sheila Adey HT MLT _ New! Open Messenger faster on the MSN homepage http://go.microsoft.com/?linkid=9677405___

[Histonet] Uranyl Nitrate

I am looking for an alternate disposal method for my Uranyl Nitrate.  I was quoted at about $3000 from our haz. waste disposal company.  I would like to find a cheaper method.  I cannot ship it outside the US.  Would anyone be able to take it and add it to their waste?  I have about 500ml of a 1

RE: [Histonet] Ink issues (UNCLASSIFIED)

Classification: UNCLASSIFIED Caveats: NONE We use "Distilled White Vinegar". I buy it at the grocery store by the gallon. It is the acetic acid in bouin's solution that fixes the ink to the tissue, not the picric acid. I have been using white vinegar for more years than I care to remember. Hope

RE: [Histonet] Re: Ink issues

Maybe it is the acetic acid in Bouin's that is the "active ingredient" for dye adherence! Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.ed

RE: [Histonet] Re: Gloves

It says on the box that these aloe touch nitrile gloves are "Latex-free, powder-free nitrile examination gloves, single use, non-sterile. Tested for use with chemotherapy drugs" We've had a terrible time with getting good gloves, and then when we get some good ones, they are replaced the fol

RE: [Histonet] Re: Ink issues

I concur with the Samurai Pathologist. Long ago, we used Bouin's but have stopped that procedure and prefer to just blot dry but do also use 3% acetic acid, especially if blotting could potentially damage a delicate tissue. Good luck. Joe Galbraith University of Iowa joseph-galbra...@uiowa.edu

[Histonet] Re: Gloves

Janet Bonner notes: >We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for >small size) www.medline.com.(1-800 - medline) These gloves are not >stiff, they fit the hand 'like a glove' . They say on the box "not intended >to be used as a chemical barrier", but they

[Histonet] Re: Ink issues

About what to put on tissue to make the marking ink stick to it: I don't use anything. The trick is to blot the surfaces thoroughly dry with a paper towel before you try to put the ink on. Of course, there's nothing you can use that will make ink adhere well to cauterized surfaces (some breast bio

Re: [Histonet] Robotic Prostatectomy

And your problem will not be solved unless the prostates, after being inked, are cut in half before placing them in the fixative. I do not see any problem in cutting in half a properly inked prostate to assure proper fixation and subsequent processing. René J.  --- On Wed, 9/2/09, Phyllis Thaxt

[Histonet] Cost to produce an H&E slide

Hello Histonet.Has anyone ever figured out how much it costs to produce an H&E slide? Are there any articles out there that would help to figure this out? I realize it may be different for each institution but a ball park figure would be greatly appreciated. Thanks Amylin Johnson

[Histonet] (no subject)

Does anyone have experience with the Cell Technology Apo-single stranded DNA antibody? Please let me know if you do. Thanks! Jennifer James BS, HT(ASCP)HTL, QIHC, CRS Research Assistant Experimental Pathology Laboratory Winthrop P.Rockefeller Cancer Institute, Room 429 Universtiy of Arkansas for

[Histonet] Robotic Prostatectomy

For the first time here, we have Urologists using the robotic method for removing prostates. The PA always receives them fresh, inks them, then puts the whole prostate in alcoholic fixative (currently Zfix from Anatech). The next day they are grossed in and processed.  The prostates we have rece

RE: [Histonet] H&E preparation

the good ole days for sure - purple ceilings, purple walls, purple people And cigarette ashes in the trash and food on the counter... Is it Friday yet?!!! J:>) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On B

RE: [Histonet] Ink issues

We use acetic acid - 3-5% solution (vinegar). j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 07:03 To: histonet@lists.utsouthwestern.edu Subject: [

[Histonet] RE: Ink issues

Good Morning, We dip the inked tissue in a container of Acetone. We use the 7-dye kit from Bradley Products, Inc. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -Original Message--

RE: [Histonet] Gloves

We use Aloe Touch Nitrile gloves, powder-free from Medline (MDS195084 for small size) www.medline.com.(1-800 - medline) These gloves are not stiff, they fit the hand 'like a glove' . They say on the box "not intended to be used as a chemical barrier", but they do a great job when expos

[Histonet] RE: Ink issues

My Docs use acetone. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues G

[Histonet] Ink issues

Good Morning all- I am having issues getting ink to "stick" to the tissue surface, the black and blue that we are using are fine we have tried several different companies and even use a "ink stay" acetic acid spray on the tissue. My new pathologist has talked about dipping the tissue after it is

RE: [Histonet] H&E preparation

Ah, the good 'ol days! Remember adding the oxidizinng agent (mecuric oxide) too soon when it was still bubbling and having a volcano of hematoxylin?!!! LOLj Was remembering tho', that every day, before using, we would "top off" (add...oh, about 30ml) of fresh to the filtered used batch. This rea

RE: [Histonet] H&E preparation

Ripening in the sun (we do get some in the UK) used to give Haematoxylin that was stable; if you forgot to make any up then you hastely added oxidising agent to a new batch or 'jumped started' a batch that was as yet still immature. That batch then never seemed to last as long as you got muddy stai