[Histonet] PT tech in Santa Rosa
I'm still looking for a p/t tech to run a new GI path lab in Santa Rosa. Send resumes to tja...@yahoo.com. Great facility, flexible hours, friendly docs, warm, caring staff, and competitive pay. Timothy Garcia-Jay Pillar Consulting ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] unsubscribe
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, 20 May 2010 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Freezing spray artifact (Shirley A. Powell) 2. RE: IHC Validation on new instrument (Liz Chlipala) 3. Re: Freezing spray artifact (Rena Fail) 4. RE: Freezing spray artifact (Sebree Linda A) 5. IHC Validation on microwaved tissue (Matt Brooks) 6. charging for cytospins (Tench, Bill) 7. Re: Re: Grossing Technician Qualifications (Daniel Schneider) 8. Temp Histo Tech Needed (Marcia Fisher) 9. IHC Validation on new instrument (Troutman, Kenneth A) 10. PPE (Cindy DeRiso) 11. RE: Freezing spray artifact (sgoe...@xbiotech.com) 12. RE: Re: Grossing Technician Qualifications (Mahoney,Janice A) 13. RE: PPE (Liz Chlipala) 14. RE: PPE (Sherwood, Margaret ) 15. Re: Re: Grossing Technician Qualifications (Daniel Schneider) 16. IHC Validation (again) (Laurie Colbert) 17. Re: alternative for bunsen burner? (Debbie Dreesen) 18. Thomas Crowell is out of the office. (thomas.crow...@novartis.com) 19. RE: IHC Validation (again) (Liz Chlipala) 20. Re: Freezing spray artifact (James L Burchette) 21. Equipment Repair Dude (sgoe...@xbiotech.com) 22. aanlktik8dnsqt16rpmss8_x5fgqqe_18-ctuenp71...@mail.gmail.com (Andrew Burgeson) 23. Histonet (Rebecca Johnson) 24. bone processing (Andrew Burgeson) 25. Re: Grossing Technician Qualifications (Robert Richmond) 26. RE: Freezing spray artifact (Jennifer MacDonald) -- Message: 1 Date: Wed, 19 May 2010 13:15:37 -0400 From: Shirley A. Powell powell...@mercer.edu Subject: [Histonet] RE: Freezing spray artifact To: Martin, Erin erin.mar...@ucsf.edu, histonet histonet@lists.utsouthwestern.edu Message-ID: 9bf995bc0e47744e9673a41486e24ee2268c551...@mercermail.merceru.local Content-Type: text/plain; charset=us-ascii There is a text written by the late Lee Luna and Samuel Wesley Thompson entitled An Atlas of Artifacts in this the artifact from freeze spray is pictured. The ISBN # is 0-398-03624-1 Published by Charles C. Thomas, Publisher. Shirley Powell -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, May 19, 2010 12:09 PM To: histonet Subject: [Histonet] Freezing spray artifact Has anyone run into a problem or artifact from freezing spray? I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like. Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Wed, 19 May 2010 11:21:32 -0600 From: Liz Chlipala l...@premierlab.com Subject: RE: [Histonet] IHC Validation on new instrument To: Laurie Colbert laurie.colb...@huntingtonhospital.com, histonet@lists.utsouthwestern.edu Message-ID: ee33be5c905a3046a7ff8f58a64c8e4b101...@server.premierlab.local Content-Type: text/plain; charset=us-ascii We perform our entire validation process as a new piece of equipment. Our validation protocols are quite extensive, up to about 85 pages long on each piece of major equipment, at least that's what it was for our new prisma stainer and glass coverslipper. We perform an installation/operational qualification protocol or an IOQ. If we move the instrument we also do the same thing, we already have the protocol written which takes most of the time we just execute it again. We are a GLP lab so we work off a Validation Master Plan that basically tells us how we are going to validate each piece of equipment in the lab. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu
Re: [Histonet] aanlktik8dnsqt16rpmss8_x5fgqqe_18-ctuenp71...@mail.gmail.com
My wrongI meant epidermis. Blame old age On Thu, May 20, 2010 at 12:08 AM, Andrew Burgeson nap...@siscom.net wrote: One thing i forgot to mention wasthat when you embed, try to orientate the tissue so that the long axis (if there is one) lies in the same direction as the cutting stroke. when embedding, orientate the tissue at a slight diagonal, so that the knife dous not continously pass through the tissue on the cutting stroke - (this works well for skins also, except make sure the dermis is away from the knife) I do not agree with the above statement about the dermis being embedded so as to be facing away from the blade. The last tissue to hit the knife edge should be EPIDERMIS. Dermis and SubQ fat should be the first tissues to hit the blade. Perhaps this is what you meant by dermis? Otherwise, I would agree with that methodology of orientation and angle. MethylMethacrylate bone embedding works very well from what I understand. See link: http://www.jhc.org/cgi/content/full/45/2/307 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Linda Blazek
Please contact me at this email address. Thank you. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 595-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Grossing Technician Qualifications
At 9:00 PM -0400 5/19/10, Robert Richmond wrote: I probably shouldn't be admitting it, but I don't think I had enough college science courses to be allowed to gross today. Maybe if they don't find out that two of my biology courses were in paleontology. I can gross a trilobite like you wouldn't believe! Hah!! If it is defined as college, I took minimal premed courses and majored in classics and literature. I guess I cannot gross either. My attitude is screw the pundits. I will supervise in my own way. It is what 'professional' means. BB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] rfp in cryosections
Hello, I am currently making cryosections of transgenic tomato fruit expressing mRFP http://www.invitrogen.com/site/us/en/home/support/Product-Technical-Resources/Product-Spectra.mRFP.html but am having some problems visualising fluorescence with a confocal. I realise fluorescent proteins work best in live cells but I have read a few reports of direct GFP detection in fixed cryosections. When I view hand sections of the fruit I can easily see the flourescence with no autofluorescence in my wild type control. However, with my cryosections the autofluorescence increases and the RFP fluorescence is so diminished i can't distinguish it. I've tried three different fixatives (in order of most to least autofluorescence: 4% formaldehyde/0.25% glutaraldehyde in PBS, 3:1 ethanol:acetic acid and 1:3 ethanol:acetic acid), followed by infiltration with 20% sucrose in PBS. I embed in OCT and use the cryojane tape transfer system to transfer the sections to slides. After sectioning at 14 microns I dry the slides at room temperature before viewing. I assume some part of the fixation process (and/or the cryojane tape transfer process) is leading to autofluorescence in the RFP range. Does anyone have any suggestions for minimizing this? Thanks in advance, Richard -- Richard Pattison Boyce Thompson Institute for Plant Research Tower Road Ithaca, NY, 14853-1801 USA Phone: +1 607 254 8757 rjp...@cornell.edu http://bti.cornell.edu/CarmenCatala.php ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
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RE: [Histonet] PPE
We require gloves for sterile sectioning as we are usually taking sections for DNA and RNA (and a mask).Otherwise gloves etc are optional. Nothing for embedding. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cindy DeRiso Sent: Wednesday, May 19, 2010 1:21 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] PPE Does any one use or require gloves when cutting and embedding? How about safety goggles when embedding? Thanks in advance- Cindy DeRiso Yale University Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Large fibrous Bone
I would like to thank everyone for their contribution on how to remedy our large fibrous bone tissue. Just for your knowledge, this case is a Congenital Pseudoathrosis(CPT) and we have process them both for paraffin and MMA. The paraffin was the choice for our study since we will be doing a lot of IHC studies on this. I was able to cut this hard fibrous tissue by placing them longer(1 hr) on ice and constanly surface decal (30minutes) even if a complete decal was achieve. Thank you histonetters for your unselfish knowledge and experience. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sodium borate?
Help! Does anyone know a substitute for sodium borate in the GMS stain? Ours got unknowingly thrown out by our out-date police and he didn't tell anyone, and now we need it ASAP. And we don't have half of the stuff for the Warthin-Starry either. (Derm lab. We do this stuff oh so often, but now of course it is a stat case.) Or if anyone knows a halfway easy stain for, I'm assuming, spirochetes. Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] differentiation of optic tectum layers
Hello, I want to stain sections of optic tectum to show the different cellular layers. I am planning on using cresyl violet and luxol fast blue. Anyone have any better ideas? I'm going to try HE as well simply because everyone knows it. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@gmail.com tel: +27-84-632-1925 (c) Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sodium borate?
20 Mule Team Borax in the laundry soap aisle. ;) From: Ingles Claire cing...@uwhealth.org To: histonet@lists.utsouthwestern.edu Sent: Thu, May 20, 2010 9:47:50 AM Subject: [Histonet] Sodium borate? Help! Does anyone know a substitute for sodium borate in the GMS stain? Ours got unknowingly thrown out by our out-date police and he didn't tell anyone, and now we need it ASAP. And we don't have half of the stuff for the Warthin-Starry either. (Derm lab. We do this stuff oh so often, but now of course it is a stat case.) Or if anyone knows a halfway easy stain for, I'm assuming, spirochetes. Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job opening in Dallas, Texas
HISTOTECHNOLOGIST ProPath, a high volume, pathology practice, located in Dallas, Texas, has an immediate opening for a Histotechnologist. Responsibilities include embedding tissue specimens, microtomy of paraffin-embedded tissue, operation of automated stainer and coverslipper, equipment maintenance and record retention. The ideal candidate will have a high school diploma or equivalent. We prefer, HT, HTL (ASCP) registered or eligible. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! For consideration send resume to: ProPath, Human Resources 1355 River Bend Drive Dallas, TX 75247 FAX: 214/237-1825 Job-Line: 214/237-1775 Email address: j...@propath.com Website: www.propath.com http://www.propath.com/ Don't Follow the Leader! Join the Leader! EOE Doug Showers, MS, HT Histology Manager ProPath 1355 River Bend Dallas, TX 75247 214-237-1680 214-422-3083 Mobile To learn more about ProPath, please visit http://www.ProPathLab.com http://www.propathlab.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: alternative for bunsen burner
TBS has an electric 3 well forceps warmer catalog #FW-120 Message: 3 Date: Tue, 18 May 2010 10:26:17 -0700 From: Pat Laurie foreig...@gmail.com Subject: Re: [Histonet] alternative for bunsen burner? To: Brandi Higgins brandihigg...@gmail.com Cc: histonet@lists.utsouthwestern.edu Message-ID: aanlktillsw0rymnowr8yjbdgle4byz78bbusp9hh6...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 We use one of the Bactincinerator sterilizer that Micro uses. I believe we got ours through cardinal. On Tue, May 18, 2010 at 8:30 AM, Brandi Higgins brandihigg...@gmail.comwrote: Hello all, Our hospital is moving our histology department across the hall and have asked us if we can find an alternative to the bunsen burner so they don't have to install a gas line. The pathologist has told me that there is some machine that can be used instead of a flame to burn the forceps while embedding (which is our main use for the bunsen flame). This is the only lab I have ever worked at and I don't know what such a machine would be called. If anyone knows the name, or any other alternative method can you let me know. Model numbers/companies would be a bonus too! Thanks so much for your help. Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mounting Medium for Immunofluorescence
We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium. By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides. This mounting medium is described as Mounting medium for fluorescence, with propidium iodide. Will this make a difference when reading the slides?? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Mounting Medium for Immunofluorescence
I think that the propidium iodide will stain the nuclei or DNA, similar to DAPI, so this mounting media will counterstain the nuclei and its visible in the FITC wavelength (488), while if you conterstain with DAPI it is excited with a different wavelength, therefore it might interfere with the FITC portion of your stain. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, May 20, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mounting Medium for Immunofluorescence We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium. By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides. This mounting medium is described as Mounting medium for fluorescence, with propidium iodide. Will this make a difference when reading the slides?? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mounting Medium for Immunofluorescence
Hi Laurie, Propidium iodide is a DNA intercalator that fluoresces red (ex 488nm). Regards, Merced --On Thursday, May 20, 2010 11:13 AM -0700 Laurie Colbert laurie.colb...@huntingtonhospital.com wrote: We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium. By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides. This mounting medium is described as Mounting medium for fluorescence, with propidium iodide. Will this make a difference when reading the slides?? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] weekend fixation
Histonetters: Here's a message I was asked to post.. Dear Colleagues, I have the following question concerning tissue processing. We do a lot of IHC work on NF fixed tissue. To standardize and minimize the effect of NF fixation, we fixate the tissue always for 24h. This is of course a problem for tissues taken on Friday. In the past, we asked our technicians to come on Saturday to embed the tissues in paraffin. Unfortunately, this is not possible anymore, and that is why I need your advice. What would you suggest ? 1) to leave the tissue in NF until Sunday evening and start processing, or 2) to keep the fixation time (24 hours) and leave the tissue in alcohol 70% until Sunday evening and then start processing. Thanks for your advice. Kind regards, Wim. Prof. dr. Wim Van den Broeck, DVM, MSc, PhD Cell biology and Histology Department of Morphology - Faculty of Veterinary Medicine Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Mounting Medium for Immunofluorescence
Actually if I could make a minor correction to your statement: propidium iodide is excited by green light at 488nm but emits in the red portion of the spectrum (620nm)... --On Thursday, May 20, 2010 12:48 PM -0600 Liz Chlipala l...@premierlab.com wrote: I think that the propidium iodide will stain the nuclei or DNA, similar to DAPI, so this mounting media will counterstain the nuclei and its visible in the FITC wavelength (488), while if you conterstain with DAPI it is excited with a different wavelength, therefore it might interfere with the FITC portion of your stain. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, May 20, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mounting Medium for Immunofluorescence We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium. By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides. This mounting medium is described as Mounting medium for fluorescence, with propidium iodide. Will this make a difference when reading the slides?? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PBA for Immunofluorescence
Concerning Immunofluorescence, where are you getting your (PBA) Protein Blocking Agent from? I use to order it from Thermo Shandon but they no longer carry it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, May 20, 2010 1:49 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mounting Medium for Immunofluorescence I think that the propidium iodide will stain the nuclei or DNA, similar to DAPI, so this mounting media will counterstain the nuclei and its visible in the FITC wavelength (488), while if you conterstain with DAPI it is excited with a different wavelength, therefore it might interfere with the FITC portion of your stain. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, May 20, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mounting Medium for Immunofluorescence We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium. By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides. This mounting medium is described as Mounting medium for fluorescence, with propidium iodide. Will this make a difference when reading the slides?? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: PBA for Immunofluorescence
We just use Dako's serum free protein block for IF. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: Nails, Felton [mailto:flna...@texaschildrens.org] Sent: Thursday, May 20, 2010 1:29 PM To: Liz Chlipala; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: PBA for Immunofluorescence Concerning Immunofluorescence, where are you getting your (PBA) Protein Blocking Agent from? I use to order it from Thermo Shandon but they no longer carry it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, May 20, 2010 1:49 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mounting Medium for Immunofluorescence I think that the propidium iodide will stain the nuclei or DNA, similar to DAPI, so this mounting media will counterstain the nuclei and its visible in the FITC wavelength (488), while if you conterstain with DAPI it is excited with a different wavelength, therefore it might interfere with the FITC portion of your stain. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, May 20, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mounting Medium for Immunofluorescence We normally coverslip our immunofluorescence slides with a permanent aqueous mounting medium. By mistake, today they were coverslipped with a mounting medium that we use for coverslipping our FISH slides. This mounting medium is described as Mounting medium for fluorescence, with propidium iodide. Will this make a difference when reading the slides?? Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] possible AFB contaminant
Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are false positives on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain. We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths. Any advice or comments would be greatly appreciated. Thanks Debi This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mounting medium for IF
Hello Laurie Pi gives red color in IF, it stains DNA and RNA PI excites at 535nmand emits at 615nm, producing a *red* fluorescence. Therefore if you are using any chromophore at these wave lengths which emits red color you will have problems. By the way we also have several mounting mediums for IHC and IF, if you would like to try, please let us know. Bader ** -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience corp. Phone: + 425 367 4601 Phone: + 425 514 3761 Fax: + 425 367 4817 cell (mobile) phone: + 425 314 0199 e-mail address: bade...@gmail.com Web site: www.immunobioscience.com Marketing: phone: + 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] weekend fixation
I was talking to Peggy Wenk over the weekend at the MSH meeting and they had a paper that was published regarding fixation and ER/PR staining sensitivity etc. The biggest problem that they reported is underfixation is much worse than over fixation. I think a minimum of 10 hours of fixation demonstrated good results and that intensity of staining started to decrease but not by much at 48 hours. I would be more concerned over underfixation than overfixation. Also the new ER/PR guidelines state its acceptable to have samples in fixative for 72 hours. Maybe Peggy can post the link to this paper. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, May 20, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weekend fixation Histonetters: Here's a message I was asked to post.. Dear Colleagues, I have the following question concerning tissue processing. We do a lot of IHC work on NF fixed tissue. To standardize and minimize the effect of NF fixation, we fixate the tissue always for 24h. This is of course a problem for tissues taken on Friday. In the past, we asked our technicians to come on Saturday to embed the tissues in paraffin. Unfortunately, this is not possible anymore, and that is why I need your advice. What would you suggest ? 1) to leave the tissue in NF until Sunday evening and start processing, or 2) to keep the fixation time (24 hours) and leave the tissue in alcohol 70% until Sunday evening and then start processing. Thanks for your advice. Kind regards, Wim. Prof. dr. Wim Van den Broeck, DVM, MSc, PhD Cell biology and Histology Department of Morphology - Faculty of Veterinary Medicine Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] weekend fixation
From what I have seen and heard you can have fixation times up to= 72
hours and still be ok?
Sarah Goebel, B.A., HT (ASCP)
= div
Histo= technician
XBiotech USA Inc.
8201 East Rivers= ide Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: [Histonet] weekend fixation
From: LINDA MARGRAF linda.marg...@childrens.com
Date: Thu, May 20, 2010 12:04 pm
To: histonet@lists.utsouthwestern.edu
Histonetters:
Here's a message I was asked to post..
Dear Colleagues,
I have the following question concerning tissue processing. We do a
lot o= f IHC work on NF fixed tissue. To standardize and minimize the
effect of NF= fixation, we fixate the tissue always for 24h. This is
of course a problem= for tissues taken on Friday. In the past, we
asked our technicians to come= on Saturday to embed the tissues in
paraffin. Unfortunately, this is not p= ossible anymore, and that is
why I need your advice. What would you suggest= ? 1) to leave the
tissue in NF until Sunday evening and start processing, = or 2) to
keep the fixation time (24 hours) and leave the tissue in alcohol70%
until Sunday evening and then start processing.
Thanks for your advice.
Kind regards,
Wim.
Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM
Please consider the environment before printing this e-mail.
This e-mail, facsimile, or letter and any files or attachments
transmitted = with it contains
information that is confidential and privileged. This information is
intend= ed only for the
use of the individual(s) and entity(ies) to whom it is addressed. If
you ar= e the intended
recipient, further disclosures are prohibited without proper
authorization.= If you are not
the intended recipient, any disclosure, copying, printing, or use of
this i= nformation is
strictly prohibited and possibly a violation of federal or state law
and re= gulations. If you
have received this information in error, please notify Children's
Medical C= enter Dallas
immediately at 214-456- or via e-mail at priv...@childrens.com.
Childre= n's Medical
Center Dallas and its affiliates hereby claim all applicable
privileges rel= ated to this
information.
___
Histonet mailing list
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Re: [Histonet] possible AFB contaminant
How often are you decontaminating your NeXes machines (running decontaminating reagents through the tubing)? Jan Shivers UMN VetDiagLab - Original Message - From: debra.or...@uchospitals.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 20, 2010 2:41 PM Subject: [Histonet] possible AFB contaminant Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are false positives on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain. We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths. Any advice or comments would be greatly appreciated. Thanks Debi This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] weekend fixation
We fix all specimens in NBF for 24h, wash them in tap water and store them in alcohol 70% at 4°C until further processing. That works perfectly well for us, and we never noticed any loss of staining. But I also knew that some colleagues prefer to run a weekend program on their Tissue Tek without delay so that the specimens stay infiltrated with paraffin until Monday morning. Alexandra Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] weekend fixation
I just realized the question came from Belgium. I have no idea how they do things there. Mark On Thu, May 20, 2010 at 2:14 PM, Mark Tarango marktara...@gmail.com wrote: Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if you do HER2 the maximum is still 48 hours. I'm assuming you want HER2 as well, so your best option would probably be to hold the tissues in 70% alcochol on the processer until Sunday night. Mark Tarango On Thu, May 20, 2010 at 12:54 PM, Liz Chlipala l...@premierlab.comwrote: I was talking to Peggy Wenk over the weekend at the MSH meeting and they had a paper that was published regarding fixation and ER/PR staining sensitivity etc. The biggest problem that they reported is underfixation is much worse than over fixation. I think a minimum of 10 hours of fixation demonstrated good results and that intensity of staining started to decrease but not by much at 48 hours. I would be more concerned over underfixation than overfixation. Also the new ER/PR guidelines state its acceptable to have samples in fixative for 72 hours. Maybe Peggy can post the link to this paper. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, May 20, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weekend fixation Histonetters: Here's a message I was asked to post.. Dear Colleagues, I have the following question concerning tissue processing. We do a lot of IHC work on NF fixed tissue. To standardize and minimize the effect of NF fixation, we fixate the tissue always for 24h. This is of course a problem for tissues taken on Friday. In the past, we asked our technicians to come on Saturday to embed the tissues in paraffin. Unfortunately, this is not possible anymore, and that is why I need your advice. What would you suggest ? 1) to leave the tissue in NF until Sunday evening and start processing, or 2) to keep the fixation time (24 hours) and leave the tissue in alcohol 70% until Sunday evening and then start processing. Thanks for your advice. Kind regards, Wim. Prof. dr. Wim Van den Broeck, DVM, MSc, PhD Cell biology and Histology Department of Morphology - Faculty of Veterinary Medicine Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] weekend fixation
Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if you do HER2 the maximum is still 48 hours. I'm assuming you want HER2 as well, so your best option would probably be to hold the tissues in 70% alcochol on the processer until Sunday night. Mark Tarango On Thu, May 20, 2010 at 12:54 PM, Liz Chlipala l...@premierlab.com wrote: I was talking to Peggy Wenk over the weekend at the MSH meeting and they had a paper that was published regarding fixation and ER/PR staining sensitivity etc. The biggest problem that they reported is underfixation is much worse than over fixation. I think a minimum of 10 hours of fixation demonstrated good results and that intensity of staining started to decrease but not by much at 48 hours. I would be more concerned over underfixation than overfixation. Also the new ER/PR guidelines state its acceptable to have samples in fixative for 72 hours. Maybe Peggy can post the link to this paper. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, May 20, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weekend fixation Histonetters: Here's a message I was asked to post.. Dear Colleagues, I have the following question concerning tissue processing. We do a lot of IHC work on NF fixed tissue. To standardize and minimize the effect of NF fixation, we fixate the tissue always for 24h. This is of course a problem for tissues taken on Friday. In the past, we asked our technicians to come on Saturday to embed the tissues in paraffin. Unfortunately, this is not possible anymore, and that is why I need your advice. What would you suggest ? 1) to leave the tissue in NF until Sunday evening and start processing, or 2) to keep the fixation time (24 hours) and leave the tissue in alcohol 70% until Sunday evening and then start processing. Thanks for your advice. Kind regards, Wim. Prof. dr. Wim Van den Broeck, DVM, MSc, PhD Cell biology and Histology Department of Morphology - Faculty of Veterinary Medicine Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] possible AFB contaminant
We decontame once a month. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Thursday, May 20, 2010 3:23 PM To: debra.or...@uchospitals.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] possible AFB contaminant How often are you decontaminating your NeXes machines (running decontaminating reagents through the tubing)? Jan Shivers UMN VetDiagLab - Original Message - From: debra.or...@uchospitals.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 20, 2010 2:41 PM Subject: [Histonet] possible AFB contaminant Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are false positives on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain. We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths. Any advice or comments would be greatly appreciated. Thanks Debi This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] weekend fixation
Yeah. Good luck with that one. The minimum clearly states 6 hrs.
Mike
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@xbiotech.com
Sent: Thursday, May 20, 2010 3:08 PM
To: LINDA MARGRAF
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] weekend fixation
From what I have seen and heard you can have fixation times up to=2
hours and still be ok?
Sarah Goebel, B.A., HT (ASCP)
=iv
Histo=echnician
XBiotech USA Inc.
8201 East Rivers=de Dr. Bldg 4 Suite 100
Austin, Texas 78744
(512)386-5107
Original Message
Subject: [Histonet] weekend fixation
From: LINDA MARGRAF linda.marg...@childrens.com
Date: Thu, May 20, 2010 12:04 pm
To: histonet@lists.utsouthwestern.edu
Histonetters:
Here's a message I was asked to post..
Dear Colleagues,
I have the following question concerning tissue processing. We do
a
lot o= IHC work on NF fixed tissue. To standardize and minimize the
effect of NF=ixation, we fixate the tissue always for 24h. This is
of course a problem=for tissues taken on Friday. In the past, we
asked our technicians to come=n Saturday to embed the tissues in
paraffin. Unfortunately, this is not p=ssible anymore, and that is
why I need your advice. What would you suggest= 1) to leave the
tissue in NF until Sunday evening and start processing, =r 2) to
keep the fixation time (24 hours) and leave the tissue in alcohol
70% until Sunday evening and then start processing.
Thanks for your advice.
Kind regards,
Wim.
Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM
Please consider the environment before printing this e-mail.
This e-mail, facsimile, or letter and any files or
attachments
transmitted =ith it contains
information that is confidential and privileged. This information
is
intend=d only for the
use of the individual(s) and entity(ies) to whom it is addressed.
If
you ar= the intended
recipient, further disclosures are prohibited without
proper
authorization.=f you are not
the intended recipient, any disclosure, copying, printing, or use
of
this i=formation is
strictly prohibited and possibly a violation of federal or state
law
and re=ulations. If you
have received this information in error, please notify
Children's
Medical C=nter Dallas
immediately at 214-456- or via e-mail at
priv...@childrens.com.
Childre='s Medical
Center Dallas and its affiliates hereby claim all
applicable
privileges rel=ted to this
information.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
[1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet=r
References
1.
3Dhttp://lists.utsouthwestern.edu/mailman/listin___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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RE: [Histonet] weekend fixation
The problem with all this is that the ER/PR fixation times and the her2 fixation times do not match 72 hrs vs 48 hrs. Many times the same specimen that receives ER/PR also gets her2. This is where things are missed up. Why would they ever change one and not the othe...@#$%^* Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, May 20, 2010 2:54 PM To: LINDA MARGRAF; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] weekend fixation I was talking to Peggy Wenk over the weekend at the MSH meeting and they had a paper that was published regarding fixation and ER/PR staining sensitivity etc. The biggest problem that they reported is underfixation is much worse than over fixation. I think a minimum of 10 hours of fixation demonstrated good results and that intensity of staining started to decrease but not by much at 48 hours. I would be more concerned over underfixation than overfixation. Also the new ER/PR guidelines state its acceptable to have samples in fixative for 72 hours. Maybe Peggy can post the link to this paper. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, May 20, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weekend fixation Histonetters: Here's a message I was asked to post.. Dear Colleagues, I have the following question concerning tissue processing. We do a lot of IHC work on NF fixed tissue. To standardize and minimize the effect of NF fixation, we fixate the tissue always for 24h. This is of course a problem for tissues taken on Friday. In the past, we asked our technicians to come on Saturday to embed the tissues in paraffin. Unfortunately, this is not possible anymore, and that is why I need your advice. What would you suggest ? 1) to leave the tissue in NF until Sunday evening and start processing, or 2) to keep the fixation time (24 hours) and leave the tissue in alcohol 70% until Sunday evening and then start processing. Thanks for your advice. Kind regards, Wim. Prof. dr. Wim Van den Broeck, DVM, MSc, PhD Cell biology and Histology Department of Morphology - Faculty of Veterinary Medicine Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] possible AFB contaminant
Are you using tubing on your dispensers for your DI water? They can be a source of contaminant. You can also have cross contaminantion from your control slide during dehydration and hydration as well as during staining.Do not use the same set up for contrpl and pt slide. Lay slides flat for hand staining Rena Fail - Original Message From: debra.or...@uchospitals.edu debra.or...@uchospitals.edu To: histonet@lists.utsouthwestern.edu Sent: Thu, May 20, 2010 3:41:09 PM Subject: [Histonet] possible AFB contaminant Please help, we have had an increase in demands for repeats on our AFB stain. Our pathologists have been noticing what they believe are false positives on our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. Because of this problem, we have been doing side by side comparisons using the Nexus and hand stain. We still have complaints. We have made new reagents, changed kits, tested our DI water sytem and have different techs cutting using different waterbaths. Any advice or comments would be greatly appreciated. Thanks Debi This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] weekend fixation
I heard on a teleconference yesterday that they're going to be changing both to 72 hours max time fixation, but until its the published guidlines 48 hours still stands for her2neu. Mark On Thu, May 20, 2010 at 2:28 PM, Mike Pence mpe...@grhs.net wrote: The problem with all this is that the ER/PR fixation times and the her2 fixation times do not match 72 hrs vs 48 hrs. Many times the same specimen that receives ER/PR also gets her2. This is where things are missed up. Why would they ever change one and not the othe...@#$%^* Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, May 20, 2010 2:54 PM To: LINDA MARGRAF; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] weekend fixation I was talking to Peggy Wenk over the weekend at the MSH meeting and they had a paper that was published regarding fixation and ER/PR staining sensitivity etc. The biggest problem that they reported is underfixation is much worse than over fixation. I think a minimum of 10 hours of fixation demonstrated good results and that intensity of staining started to decrease but not by much at 48 hours. I would be more concerned over underfixation than overfixation. Also the new ER/PR guidelines state its acceptable to have samples in fixative for 72 hours. Maybe Peggy can post the link to this paper. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, May 20, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weekend fixation Histonetters: Here's a message I was asked to post.. Dear Colleagues, I have the following question concerning tissue processing. We do a lot of IHC work on NF fixed tissue. To standardize and minimize the effect of NF fixation, we fixate the tissue always for 24h. This is of course a problem for tissues taken on Friday. In the past, we asked our technicians to come on Saturday to embed the tissues in paraffin. Unfortunately, this is not possible anymore, and that is why I need your advice. What would you suggest ? 1) to leave the tissue in NF until Sunday evening and start processing, or 2) to keep the fixation time (24 hours) and leave the tissue in alcohol 70% until Sunday evening and then start processing. Thanks for your advice. Kind regards, Wim. Prof. dr. Wim Van den Broeck, DVM, MSc, PhD Cell biology and Histology Department of Morphology - Faculty of Veterinary Medicine Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Career Opportunities
Hi Histonet Subscribers, Are you interested in hearing about new job opportunities? I am a one of the founders of a Healthcare Recruiting firm that specializes in placing Lab Professionals. We work exclusively on permanent positions and have established relationships with clients at leading hospitals and labs across the country. We are completely free of charge to candidates and are currently working on numerous Histology positions. Our clients often assist with relocation expenses. Below is a list of some of the Histology opportunities we are currently working on. *Southern GA - Histology Supervisor 1st shift *Atlanta, GA - Atlanta - Histotech *Atlanta, GA - Atlanta - Pathology Coordinator (HT or CT) *New York, NY - Histotech 3rd shift *New York, NY - Histology Supervisor *Upstate NY (Capital District) - Histotechnologist 1st shift *Las Vegas, NV - Histotech 3rd shift *Las Vegas, NV - Histology Supervisor - 3rd shift *Palm Springs, CA - Histotech - 1st shift *Los Angeles, CA - Histotech *Northeastern VA - Histotech 2nd shift *Southern NH - HTL and HT *Southeastern MA - IHC Tech *Southeastern MA - Histotech If you're interested in learning more about any of these opportunities then please email me a resume and let me know how best to get in touch with you. If none of these are a fit please let me know what you'd be interested in and where you're looking so I can tailor a search for you. With the New Year upon us many of our clients have fresh hiring budgets and will be looking to add people over the next several months. We work on positions at all levels and cover the entire US. To view some additional opportunities please visit our website at www.ka-recruiting.com . Sincerely, KC Carpenter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 k...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: weekend fixation
The CAP has the following to say about this issue (in the FAQ section on HE= R2 testing): The CAP Immu= nohistochemistry Committee does not advocate transferring formalin-fi= xed tissues into alcohol. There are no data to support the validity= of testing specimens processed in this manner, and there is a signif= icant risk that incompletely fixed tissues will be placed in alcohol prematurely and tissue fixation will be completed in alcohol. This = may be more deleterious to IHC testing than slightly prolonged formal= in fixation. Data are also lacking on testing specimens that have h= ad prolonged exposure to xylene (orxylenesubstitute).Finally,it=isconsidered unacceptable to leave fully processed tissues in heate= d paraffin for prolonged periods. Confident= iality Notice: This email message, including any attachments, is for the = sole use of the intended recipient(s) and may contain confidential and pr= ivileged information. Any unauthorized review, use, disclosure or distr= ibution is prohibited. If you are not the intended recipient, please co ntact the sender by reply email and destroy all copies of the original me= ssage. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: weekend fixation
We discussed this problem of weekend fixation of breast tissue (about 54 hrs in 10% NBF) with our Reference Lab and they reassured us that ER/PR IHC wouldn't be a problem. However, when ordering Her2, select FISH method instead of IHC. (Otherwise, if it is fixed for less than 48 hrs we would order Her2 by IHC, then reflex 2+ to FISH). Any thoughts about this? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] weekend fixation
Dear Colleagues: Please keep in mind that the ASCO/CAP guidelines are recommendations, not mandates. You can experiment, validate, and establish your own formalin-fixation protocol for breast specimens. At Hartford Hospital I have established a minimum of 4.5 hours for needle core specimens. This includes 0.5 hour of pre-processor fixation and 4 hours of fixation on our tissue processors. We do use the 6 hour minimum for all excisional specimens. WE HAVE NO MAXIMUM FIXATION TIME. I can show you absolutely beautiful HER2 immunoreactivity and gene amplification on breast cancer tissues that have been fixed for 1 week, 1 month, 1 year, and longer. If you don't agree with the guidelines, work with your pathologist(s) and establish your own fixation policy. And, please remember, fixation time is only one piece to the puzzle. In my experience, minimizing ischemic time, making sure that the tissue does not dry out prior to fixation, and submitting thin (2 mm) sections are probably more important than fixation time. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Mike Pence 05/20/10 6:10 PM Yeah. Good luck with that one. The minimum clearly states 6 hrs. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@xbiotech.com Sent: Thursday, May 20, 2010 3:08 PM To: LINDA MARGRAF Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] weekend fixation From what I have seen and heard you can have fixation times up to=2 hours and still be ok? Sarah Goebel, B.A., HT (ASCP) =iv Histo=echnician XBiotech USA Inc. 8201 East Rivers=de Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 Original Message Subject: [Histonet] weekend fixation From: LINDA MARGRAF Date: Thu, May 20, 2010 12:04 pm To: Histonetters: Here's a message I was asked to post.. Dear Colleagues, I have the following question concerning tissue processing. We do a lot o= IHC work on NF fixed tissue. To standardize and minimize the effect of NF=ixation, we fixate the tissue always for 24h. This is of course a problem=for tissues taken on Friday. In the past, we asked our technicians to come=n Saturday to embed the tissues in paraffin. Unfortunately, this is not p=ssible anymore, and that is why I need your advice. What would you suggest= 1) to leave the tissue in NF until Sunday evening and start processing, =r 2) to keep the fixation time (24 hours) and leave the tissue in alcohol 70% until Sunday evening and then start processing. Thanks for your advice. Kind regards, Wim. Prof. dr. Wim Van den Broeck, DVM, MSc, PhD Cell biology and Histology Department of Morphology - Faculty of Veterinary Medicine Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted =ith it contains information that is confidential and privileged. This information is intend=d only for the use of the individual(s) and entity(ies) to whom it is addressed. If you ar= the intended recipient, further disclosures are prohibited without proper authorization.=f you are not the intended recipient, any disclosure, copying, printing, or use of this i=formation is strictly prohibited and possibly a violation of federal or state law and re=ulations. If you have received this information in error, please notify Children's Medical C=nter Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Childre='s Medical Center Dallas and its affiliates hereby claim all applicable privileges rel=ted to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu [1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet=r References 1. 3Dhttp://lists.utsouthwestern.edu/mailman/listin___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
