RE: [Histonet] Nail Problems
Hi Laurie, I have had success keeping the nails on using gelatin coated slides. I make up the gelatin in beaker and dissolve it using the microwave. Then coat the slide and pick the section from the water bath using the the just coated slide. I then dry the slide overnight in the 37degree oven. You may need to vary the concentration of the gelatin. My first attempts were successful because the gelatin wasn't concentrated enough. The reason I think that the nail won't stick is because you can't get it to sit flat. Also you can first float the section on cold water. I can't remember whether I picked up the section only from cold water exclusively. I think that nails have to be one of the most difficult tissues to keep on a slide. Gerard Spoelstra Veterinary Histology Murdoch University Western Australia -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of Laurie Elmgren Sent: Thu 8/12/2010 8:15 PM To: Histonet Subject: [Histonet] Nail Problems Does anyone have a sure fire way of keeping nails attached to the slide? We use Sta-On and charged slides, have used albumin, dried overnight, and still have difficulty keeping them attached during staining. Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515x1108 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Acceptable way to bake sections onto slides?
Hi In our fairly large histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to bake for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Acceptable way to bake sections onto slides?
Use the sakura DRS hotpot Loading from the plate (apart for the other drawbacks) will set the wax on the first slides put in the racks and you may end uo with inadequate dewaxing Just my 5cents worth Annieinarabia Empower your Business with BlackBerry® and Mobile Solutions from Etisalat -Original Message- From: Gibson, Philip philip.gib...@nuth.nhs.uk Sender: histonet-boun...@lists.utsouthwestern.edu Date: Fri, 13 Aug 2010 13:23:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Acceptable way to bake sections onto slides? Hi In our fairly large histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to bake for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Acceptable way to bake sections onto slides?
The correct answer is b: if there is water underneath the section that is going to be placed on the hot plate, the most likely artifact is that of empty nuclei that will ruin the sections' usefulness. René J. --- On Fri, 8/13/10, Gibson, Philip philip.gib...@nuth.nhs.uk wrote: From: Gibson, Philip philip.gib...@nuth.nhs.uk Subject: [Histonet] Acceptable way to bake sections onto slides? To: histonet@lists.utsouthwestern.edu Date: Friday, August 13, 2010, 8:23 AM Hi In our fairly large histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to bake for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C4d on paraffin sections
I need some advice. We have been trying to do C4d on paraffin sections without much success. Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much! Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Acceptable way to bake sections onto slides?
Totally agree with Rene. You will need to use some type of drying oven Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 13, 2010 8:36 AM To: histonet@lists.utsouthwestern.edu; PhilipGibson Subject: Re: [Histonet] Acceptable way to bake sections onto slides? The correct answer is b: if there is water underneath the section that is going to be placed on the hot plate, the most likely artifact is that of empty nuclei that will ruin the sections' usefulness. René J. --- On Fri, 8/13/10, Gibson, Philip philip.gib...@nuth.nhs.uk wrote: From: Gibson, Philip philip.gib...@nuth.nhs.uk Subject: [Histonet] Acceptable way to bake sections onto slides? To: histonet@lists.utsouthwestern.edu Date: Friday, August 13, 2010, 8:23 AM Hi In our fairly large histopathology lab we're hoping to consolidate our autostainers and coverslippers to only two (Sakura) machines. In order to efficiently do this, we would need to bypass the autostainer's oven so that multiple racks of slides can be processed continuously without any 10-15 minute hold-ups. Therefore, we would like our four separate microtomist teams to place freshly-cut sections on a hotplate to bake for 10 minutes, before being picked up into racks and transferred directly to xylene on the autostainer. My question: Do hotplates work well enough to do this? Two conflicting views in my lab are (a) Yes, this would work in my experience, and (b) No, this creates artifacts because water trapped underneath the sections boils and does damage. Of course, the more conventional approach would be to use ovens, but loading and unloading an oven before the autostainer is an additional wasteful step. What do my fellow histonetters think? Many Thanks Phil Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Blade Angle on Microtome
Key Kim, The blade angle depends on the manufacturer of the microtome. In general, Leica suggests and angle of 3 to 5. Microm uses 10 to 12 and Shandon uses 0 to 3. Confused? Me too and I repair them for a living. I have found that vibration is caused by problems with the knife holder more often than knife angle. The problem can be as simple as the pressure plate not being tight enough. Let me know what type of tome you have and I may me able to help. Best Matt Mincer Tech One Biomedical 708-822-3738 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: C4d on paraffin sections
We use the C4d from Cell Marque now with great results; it is an IVD. ER1 20 minutes on the BondMax and Bond III Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Friday, August 13, 2010 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d on paraffin sections I need some advice. We have been trying to do C4d on paraffin sections without much success. Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much! Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: C4d on paraffin sections
We use the same antibody from Cell Marque on the Dako Autostainers with great success. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [ronald.hous...@nationwidechildrens.org] Sent: Friday, August 13, 2010 10:29 AM To: 'Martha Ward'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: C4d on paraffin sections We use the C4d from Cell Marque now with great results; it is an IVD. ER1 20 minutes on the BondMax and Bond III Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Friday, August 13, 2010 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d on paraffin sections I need some advice. We have been trying to do C4d on paraffin sections without much success. Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much! Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Acceptable way to bake sections onto slides?
Hi Everyone Just for clarification; our current practice involves a mixture of placing racks of slides in an oven for 25 minutes, or using the hot area of an autostainer (we're talking HE's here, BTW). Occasionally, with a particularly urgent case us old-timers will place individual slides directly onto the floor of an oven for 5 mins which bakes the sections onto the slides very nicely prior to staining. My theory is that a 60-70 celsius hotplate will perform the same job for all of our HE slides. So far, I haven't noticed any particular artefacts, and we don't suffer from water pooling between section and slide anyway. I'm trying to find both the leanest and cheap (hey - this is the NHS!) solution by consolidating four stainers and three coverslippers into just the two machines. Maybe I've not looked closely enough at our super-urgent HE's... Thanks Phil Phil Gibson Senior Biomedical Scientist Histopathology Dept Royal Victoria Infirmary Newcastle Upon Tyne NE1 4LP Ext. 24565 Tel. 0191 2824565 This email has been processed by SmoothZap - www.smoothwall.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] voice recognition
We have been using Dragon with SoftPath and works well, there's a little bit of training the software at first but well worth it Bill Lecorchick Cytology Prep.Tech. 609-584-5128 Fax 609-584-6439 wleco...@rwjuhh.edu www.rwjhamilton.orghttp://www.rwjhamilton.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: voice recognition
We use Dragon here as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lecorchick, William [wleco...@rwjuhh.edu] Sent: Friday, August 13, 2010 11:41 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] voice recognition We have been using Dragon with SoftPath and works well, there's a little bit of training the software at first but well worth it Bill Lecorchick Cytology Prep.Tech. 609-584-5128 Fax 609-584-6439 wleco...@rwjuhh.edu www.rwjhamilton.orghttp://www.rwjhamilton.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Job Mohs Histotech Needed in Florida.
Hi Histonetters!, How are you? I have a exciting new opportunity I want to tell you about. I am working with a leading client located in the Sarasota area. They are looking for someone to do Mohs histology. If you are interested you would also be considered for management of the lab. You would be working 4 10 hour days. They have great benefits - 3 weeks vacation, 100% paid medical benefits for the employee and 50% for family, and 100% paid dental for employee and family and a 401K. They are a busy fast paced lab and a great group of people to work with. If you think you might be interested please let me know. I will be in the office tomorrow -Saturday. You can reach me toll free at 866-607-3542 or shoot me an e-mail with a time to call you over the weekend or on Monday. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: mailto:rel...@earthlink.net rel...@earthlink.net http://www.facebook.com www.facebook.com search Pam Barker RELIA http://www.linkedin.com/reliasolutions www.linkedin.com/reliasolutions http://www.myspace.com/pamatrelia www.myspace.com/pamatrelia http://www.twitter.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Conferences / Trade Shows
What conferences or trade shows do you attend and find most valuable? NSH? Pathology Informatics? Others? Maggie Allen Niceware International, LLC Tel (810) 629-3930 Email: maggie.al...@nicewareintl.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] please enter
Dear histonetters i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the opportunity to learn from it!! and we are ashamed to talk about that with experts like you.but the group lose its functionality in teaching IHC for the new histologiest sector. so i put our proplem on your hands and my hope is to help us to find solution an example of what i'm taking about is what is C4d and for what it is used? thanx in advance to your help and for these amazing group mohamed Faculty of Vet. Med. Cairo Univ. - Egypt We use the same antibody from Cell Marque on the Dako Autostainers with great success. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [ronald.hous...@nationwidechildrens.org] Sent: Friday, August 13, 2010 10:29 AM To: 'Martha Ward'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: C4d on paraffin sections We use the C4d from Cell Marque now with great results; it is an IVD. ER1 20 minutes on the BondMax and Bond III Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Friday, August 13, 2010 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d on paraffin sections I need some advice. We have been trying to do C4d on paraffin sections without much success. Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much! Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] please enter
Mohamed, Deposition of C4d is an inactive fragment of C4b (derived from complement component C4). It provides in-situ evidence of complement activation following alloantibody binding to graft vasculature. As such, the development of antibodies against C4d has dramatically improved the ability to diagnose antibody mediated rejection (AMR). We use the C4d (along with C3d) antibody from Cell Marque to investigate heart, lung and kidney biopsies for antibody mediated rejection in transplanted organs - in fact many consider C4d immunohistochemistry to be the gold standard for evaluation of AMR. C4d has also been postulated as a specific marker in the differential diagnosis of Follicular Lymphoma vs MALT lymphoma (Pathology - Research and Practice 2007; 203:163-167) Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, August 13, 2010 1:46 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] please enter Dear histonetters i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the opportunity to learn from it!! and we are ashamed to talk about that with experts like you.but the group lose its functionality in teaching IHC for the new histologiest sector. so i put our proplem on your hands and my hope is to help us to find solution an example of what i'm taking about is what is C4d and for what it is used? thanx in advance to your help and for these amazing group mohamed Faculty of Vet. Med. Cairo Univ. - Egypt We use the same antibody from Cell Marque on the Dako Autostainers with great success. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [ronald.hous...@nationwidechildrens.org] Sent: Friday, August 13, 2010 10:29 AM To: 'Martha Ward'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: C4d on paraffin sections We use the C4d from Cell Marque now with great results; it is an IVD. ER1 20 minutes on the BondMax and Bond III Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Friday, August 13, 2010 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d on paraffin sections I need some advice. We have been trying to do C4d on paraffin sections without much success. Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much! Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list
RE: [Histonet] voice recognition
Carol we are using Dragon with a Voice Brook interface. Dragon will work by itself but has some issues with Pathology terminology. Voice Brook was created specifically for Pathology. Our staff, PA's and Pathologists really like this configuration. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, August 12, 2010 3:02 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] voice recognition Is anyone using voice recognition with SoftPath for dictating? If so, what software program are you using and does it work well? Thank you in advance for any comments. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP and RUO's
In case anyone is still wondering, here is the response from CAP to a question about their current position on use of RUO's for diagnostic use: The CAP removed reference to RUOs in the checklist. The CAP position is RUOs are not to be used for clinical testing. Tim Morken UCSF Pathology San Francisco, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue-Tek VIP 2000-3000
I may have the chance to get a tissue processor (VIP 2000 or 3000). Are these units still serviceable? Is it hard to find parts? Are they Reliable? Thanks for any help!! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue-Tek VIP 2000-3000
I don't know the answer to your first 2 questions, but reliable they really are! René J. --- On Fri, 8/13/10, Mike Tighe mti...@trudeauinstitute.org wrote: From: Mike Tighe mti...@trudeauinstitute.org Subject: [Histonet] Tissue-Tek VIP 2000-3000 To: histonet@lists.utsouthwestern.edu Date: Friday, August 13, 2010, 4:26 PM I may have the chance to get a tissue processor (VIP 2000 or 3000). Are these units still serviceable? Is it hard to find parts? Are they Reliable? Thanks for any help!! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue-Tek VIP 2000-3000
Agreed extremely reliable -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 13, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu; Mike Tighe Subject: Re: [Histonet] Tissue-Tek VIP 2000-3000 I don't know the answer to your first 2 questions, but reliable they really are! René J. --- On Fri, 8/13/10, Mike Tighe mti...@trudeauinstitute.org wrote: From: Mike Tighe mti...@trudeauinstitute.org Subject: [Histonet] Tissue-Tek VIP 2000-3000 To: histonet@lists.utsouthwestern.edu Date: Friday, August 13, 2010, 4:26 PM I may have the chance to get a tissue processor (VIP 2000 or 3000). Are these units still serviceable? Is it hard to find parts? Are they Reliable? Thanks for any help!! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryostat cutting problems
I am new to using a cryostat and am having some issues with my tissue. 1.Every slice seems to shatter and fragment. When I have used a microtome it was because the tissue was too cold. 2. The tissue itself was flash frozen and so was not fixed and the tissue did not go through the perfusion process. 3. Each slice is condensed and doesn't lay flat. 4. The cryostat is old. Any tips would be very helpful. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cf0145ebf1eb4c4e82768d82886a0c9b8fb...@pluto.ad.murdoch.edu.au
Joe the toe Nocito...are you out there? Joe has good ideas about nails. Maybe he will send out his procedure again. I like using either potassium hydroxide 10-20% or Sodium hydroxide 10-20% for softening nail fragments before processing. Also, keep in mind that soft tissues attached are equally as important and sections of nail beds need to be of high quality. A melanoma under a nail can be a bad situation. Sometimes in addition to PAS or GMS stains for onychomycosis, we have done melanin and iron stains for areas of pigment or hemmorhagic depositions. Joe...are you out there? lol ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cf0145ebf1eb4c4e82768d82886a0c9b8fb...@pluto.ad.murdoch.edu.au
Happy Friday all, try 10% sodium hydroxide for 1 hour prior to processing. It is important to let the slides drain really well before placing them in the oven. You want to make sure there is no water between the section and the slide. We use Plus slides with plain distilled water in our water baths. Hope this helps. JTT - Original Message - From: Andrew Burgeson nap...@siscom.net To: histonet@lists.utsouthwestern.edu Sent: Friday, August 13, 2010 3:57 PM Subject: [Histonet] cf0145ebf1eb4c4e82768d82886a0c9b8fb...@pluto.ad.murdoch.edu.au Joe the toe Nocito...are you out there? Joe has good ideas about nails. Maybe he will send out his procedure again. I like using either potassium hydroxide 10-20% or Sodium hydroxide 10-20% for softening nail fragments before processing. Also, keep in mind that soft tissues attached are equally as important and sections of nail beds need to be of high quality. A melanoma under a nail can be a bad situation. Sometimes in addition to PAS or GMS stains for onychomycosis, we have done melanin and iron stains for areas of pigment or hemmorhagic depositions. Joe...are you out there? lol ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Seeking Position in Canada
I am a US trained and ASCP certified Histologist. I am looking for job opportunity in Canada either as Histologist or as an IHC Specialist. Thanking you all in advance. Emmanuel. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
