RE: [Histonet] After hours autopsies
Richard, we do autopsies regularly at the weekends (both Saturday and Sunday) and also metabolic cases as soon as we get the permit. Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, September 14, 2010 8:16 PM To: Histonet Subject: [Histonet] After hours autopsies How many of you (especially Pathology Laboratories located in or that cover Children's Hospitals) do after-hours autopsies? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] I'm in the New Lab!
We are physically moved and Organized Chaos prevails (except in Histo, of course!). There's been some discussion about what would be needed when designing a new lab and I agree with what has been offered - and actually used this sort of input from Histonet, along with the lab design course at NSH (which was priceless), and visiting a couple of labs before meeting with the architects. It was a long and arduous process but, with the exception of the chairs chosen by the majority in this building (about 700 people), was absolutely worth all the pain and agony. Please make note of our new physical address and a direct line to the histo lab (which I'll answer just as soon as I figure out how to work all those buttons and knobs!). Now to find out where I set up the microtome so I can start cutting! Good day, all! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] WS 50
HISTONETTERS I was trying my best to get out to Seattle for the workshop Steve was doing on the lab design, our budget wouldn't allow it but any help I can get would be great we are going to start construction Jan 2011 We are getting 3 patient rooms and making them one big cytology/histology lab. Is there a powerpoint or something out there to help me? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] WS 50
I would love this same information. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Wednesday, September 15, 2010 8:07 AM To: Histonet Subject: [Histonet] WS 50 HISTONETTERS I was trying my best to get out to Seattle for the workshop Steve was doing on the lab design, our budget wouldn't allow it but any help I can get would be great we are going to start construction Jan 2011 We are getting 3 patient rooms and making them one big cytology/histology lab. Is there a powerpoint or something out there to help me? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ANP. 23075
I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C3 and C5b-9
Hi fellow histonetters, I am trying to work out an assay for complement 3 and C5b-9 detection in rodent. I have moved on to mouse, but have done some work with rats also. I haven't felt all that successful in my endeavors. In mice, the tissue of interest is kidney and have thus far proceeded in the following manner: Section frozen kidneys, place on + slides, air dry overnight, fix in chilled acetone 10 min, proceed with a standard ABC protocol. I have found rabbit poly from Abcam against C5b-9 in mouse and two anti-C3 antibodies from Thermo against mouse.My results haven't been all that great and the tissue often comes off the slides, so my results are ugly and frustrating. Also, I can only go by what I am told and that is that my kidneys should be expressing complement, so I don't really have a good control. Anyone out there familiar with C3/C5b-9 detection in rodents who can offer any advise or suggestions? We would like to stay away from fluorescent procedures if possible. Any thoughts as to why I might be losing my tissue off the slides? Thank you for your help, Dolores The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] vendors send me a quote for a slide and cassette etcher
Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Mgr, OMW, Area 4, Lab 2438 301.398.4745(vm) 301.398.6360(lab) 301.398.9745(fax) To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] barcode systems
Hazel- Try calling Ventana. They were just in my lab (large reference lab in Houston) and their evaluation system includes process and space evaluation and suggestions. Barcoding speeds some areas and slows others down--nice to have them come in and show you were to save a lot of time so you get a fair trade for the extra effort for specimen integrity. I don't have his direct phone but the guy who came in was effective and our people related to him really well - Hylton Surrett was his name. Cheryl Kerry, HT(ASCP) Houston TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Problem with liver fixation
Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 with sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that im not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] dimethyl methylene blue??
Hello, A researcher wants me to do dimethyl methylene blue staining on some cartilage sections. Is anyone familiar with this? Is it just another name for standard methylene blue staining? Or is it actually another dye? If so, where can I get the dye? And, does anyone have a staining protocol they are willing to share? Paul Monfils ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Masson's trichrom - problem with nuclei staining
Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997With sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that im not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai P.S By mistake I've post this before in another message with a wrong title. please respond to that message, so the title will be o.k for future archive searching. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ANP. 23075
I simply added the requirements for daily, weekly or monthly mainenance, temp recording, cleaning under the procedure of using each piece of equipment. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 8:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Adipose Cryosections Fixation
hello! Will you either provide me with info protocol for fixation of adipose cryosections prior to staining and/or contact info for Dr. M. Rocio Sierra-Honigmann ASAP? Thank you kindly, Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] filtering cytology stains
Hello All, I was wondering how you are filtering your cytology stains. We are a relatively small lab, so we have been gravity filtering our cytology stains, but as we are getting busier with a larger volume of slides, especially fna's this is becoming a very time consuming process. I think vacuum filtration would be almost as lengthy a process. It was suggested that we look into getting some 200ml syringes that can come with an attached filter to suck in and pump out the fluid for faster filtration...is anyone using such a process? If so, do you have product names/numbers for the filters or the syringes. If anyone has another method they are using I would like to hear any suggestions. Thanks in advance for you input, Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Symphony
Hello all, I have some questions for other Symphony users out there. Can someone please contact me off-list if you don't mind answering some questions for me? Thanks much! Clare Clare J. Thornton, HTL(ASCP),QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NBF fixation and long term storage
I have some questions about Fixing tissues for paraffin embedding when the ultimate purpose will be looking for viral antigens and cell surface markers. We have some monkey tissue that has been in NBF for over a year. and we are just now processing them and embedding them in to paraffin. We will be getting additional tissues from monkey necropsies and in an effort to avoid epitope damage from prolonged exposure to NBF I was wondering if anyone could recommend a fixation period. (I.E. no longer than 24 hours in NBF, 48 hours? ETC. I was thinking of replacing the NBF (Neutral buffered Formalin) with 70% Etoh. And using 70% Etoh as long term storage of tissue parts. The ideal situation would be to get the tissues from necropsy the same day of the necropsy and store the tissues overnight in NBF and then transfer them to 70% etoh and then process them on the tissue processer after 24 hours in Etoh. I'm not sure how many animals I've eventually get and I'm a little concerned over where we will put all the spare animal parts that I'm sure I'll accumulate. We don't want to throw anything away and for some tissues I'll have more than I can put into a single cassette. I may end up using more than 1 cassette for a single piece of tissue so that I have backup cassettes of it. Anyone storing research tissues has a suggestion about the best way to store them when space is a premium. Thanks Patrick. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Problem with liver fixation
Itai, Are you post fixing in Bouins? Post fix in heated Bouins for 1hr, wash well and then follow your protocal. Hope this helps, Matt Lunetta HT (ASCP) Longmont United Hospital Message: 2 Date: Wed, 15 Sep 2010 18:27:29 +0200 From: Itai Moshe Subject: [Histonet] Re: Problem with liver fixation To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 with sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that im not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] NBF fixation and long term storage
Patrick: Under separate cover I am sending an article about NBF fixation and its comparison with other fixatives so you can make an informed decision. René J. --- On Wed, 9/15/10, Lewis, Patrick patrick.le...@seattlechildrens.org wrote: From: Lewis, Patrick patrick.le...@seattlechildrens.org Subject: [Histonet] NBF fixation and long term storage To: Histonet@lists.utsouthwestern.edu Date: Wednesday, September 15, 2010, 1:53 PM I have some questions about Fixing tissues for paraffin embedding when the ultimate purpose will be looking for viral antigens and cell surface markers. We have some monkey tissue that has been in NBF for over a year. and we are just now processing them and embedding them in to paraffin. We will be getting additional tissues from monkey necropsies and in an effort to avoid epitope damage from prolonged exposure to NBF I was wondering if anyone could recommend a fixation period. (I.E. no longer than 24 hours in NBF, 48 hours? ETC. I was thinking of replacing the NBF (Neutral buffered Formalin) with 70% Etoh. And using 70% Etoh as long term storage of tissue parts. The ideal situation would be to get the tissues from necropsy the same day of the necropsy and store the tissues overnight in NBF and then transfer them to 70% etoh and then process them on the tissue processer after 24 hours in Etoh. I'm not sure how many animals I've eventually get and I'm a little concerned over where we will put all the spare animal parts that I'm sure I'll accumulate. We don't want to throw anything away and for some tissues I'll have more than I can put into a single cassette. I may end up using more than 1 cassette for a single piece of tissue so that I have backup cassettes of it. Anyone storing research tissues has a suggestion about the best way to store them when space is a premium. Thanks Patrick. Patrick Lewis Research Associate II-Bench| Infections and Prematurity Seattle Children's Research Institute 206-884-1115 OFFICE 000-000- PAGER 000-000- CELL 206-884-7311 FAX patrick.le...@seattlechildrens.org OFFICE 1900 9th Avenue Seattle, WA 98101 MAIL M/S C9S-8, Seattle, WA 98101 WWW seattlechildrens.org http://seattlechildrens.org/ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] drying ovens
Has anyone used the TDO66 Medite drying oven? Do you like it? Is the company good to work with? Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ANP 11605 11610
HISTONETTERS I was wondering what some of your thoughts on this. Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ANP. 23075
I interpret this to mean that I, as the supervisor, need to document monthly that temps have been recorded and instrument maintenance has been recorded. I review and initial temp and maintenance charts at the end of each month to make sure that it has been done and that temps are in range. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham Sent: Wednesday, September 15, 2010 6:51 AM To: ADESUPO ADESUYI; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ANP. 23075 I would like this information also please. Kathy, Gorham, H.T. GRH National Recognition Outstanding Rural Health Organization of 2009 awarded by NRHA Gold Standard Critical Access Hospital 2009 awarded by LarsonAllen LLP Leader in Innovative Excellence 2009 awarded by the OAHHS Financial Excellence Award 2010 awarded by the national Rural Health Research Policy Analysis Center Healthcare Achievement Award for Quality in Patient Care Delivery and Satisfaction 2010 awarded by Amerinet GRH Mission We will ensure access to high-quality, cost-effective health services in a safe, customer-friendly environment for all those in need of our services. GRH Confidentiality Notice This e-mail and any attached documents are for the intended recipient/s only and should be protected against viewing by unauthorized persons. The information herein may have been disclosed from records whose confidentiality is protected by Federal and State Law. Federal regulations prohibit further distribution or copying of this information without permission. If you received this e-mail transmission in error, please notify the sender immediately to arrange for return or destruction of this information. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI Sent: Tuesday, September 14, 2010 8:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP. 23075 Hi, Does anyone have a procedure on the CAP ANP. 23075 that they would like to share? Thanking you all for your usual cooperation. Banjo Adesuyi, BS, HT (ASCP) HTL, QIHC (ASCP). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lab Supervisor/Manager Position in Orange County CA
Laboratory Supervisor /Manager preferably with CLS Qualification The Manager is a key role within the pathology organization located in Orange County California. This individual will join our expanding laboratory facilities, and will participate in daily operations of Histology, IHC, Flow, Cytogenetic and Molecular Diagnostics. The candidate will report to the CEO/Medical Director. The ideal candidate must be have previous management experience with 5-10 years practical experience in the areas of Biopsy Histology, Cytology, Bone Marrow Morphology, Flow Cytometry, IHC experience, Molecular Genetics or relevant experience. CLS certified is preferred.We are looking for an exceptional professional who enjoys the challenge of working with a cross section of professionals in a out patient-focused environment. Superior communication, collaboration, business and clinical judgment, emphasis on turnaround time, and organizational development skills are absolutely essential for success. The ideal candidate will possess exceptional interpersonal, communication, and partnering skills that will allow effective interaction with employees at all levels, with laboratory professional peers throughout the company and with clients, client services and sales professionals. Being persuasive and leading to consensus, and a commitment to the highest standards of excellence in the successful implementation of the best industry standards of the Medical Laboratory operations. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: transporting sectioned tissues
Is there any way to relax curled brain sections? They've been in cryoprotectant and I tried sitting them in PBS for a while. They're to be used in an IHC- can they be rescued? Thanks, -- Teresa Iglesias Graduate Group in Animal Behavior University of California-Davis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help! Curled cryosections
Is there any way to relax curled brain sections? They've been in cryoprotectant and I tried sitting them in PBS for a while. They're to be used in an IHC- can they be rescued? Thanks, -- Teresa Iglesias Graduate Group in Animal Behavior University of California-Davis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica-Surgipath EM Paraffin Issue
Has anyone else noticed a change in the EM400 embedding medium from Surgipath? We have not changed anything in our embedding, but now the EM400 results in cracks in the blocks. Leica says although the labeling has changed to indicate the EM400 is now also for infiltrating, the paraffin has not changed. Thanks, Sandy SVM ~ UW Madison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! Curled cryosections
Thanks, Montina The sections are 40um and I had no trouble with all my other brains (sectioned myself). I had help with these last two and they are all curled. I think they just got sectioned too fast or something. I'll try the shaker and see if it helps. Thanks! -Teresa On Wed, Sep 15, 2010 at 8:47 PM, Montina Van Meter montina.vanme...@pbrc.edu wrote: Teresa, I would put the sections through several 5-10 min. washes on a shaker table. It is very important to make sure you have all of the cryoprotectant rinsed out of the tissue or it will inhibit IHC staining. How thick are the sections? Were they cut on a cryostat or freezing microtome? I routinely cut rat brain at 40um and don't have any curling issues. That sometimes occurs when the knife has come through the section of brain too rapidly. A slow and steady motion is needed when cutting frozens. Good luck! Tina Van Meter Sent from my iPhone On Sep 15, 2010, at 9:37 PM, Teresa Iglesias tligles...@ucdavis.edu wrote: -- __ Teresa Iglesias Graduate Group in Animal Behavior Department of Evolution and Ecology University of California-Davis One Shields Avenue 2320 Storer Hall Davis, CA 95616 Office: 530-754-7837 Fax: 530-752-1449 tligles...@ucdavis.edu __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Picrosirius Red Staining of mixed archaeological residues. [SEC=UNCLASSIFIED]
Hello Histonetters, I have been using Picrosirius Red stain to detect collagen fibers in residues lifted from archaeological artefacts. The protocol has been adapted for staining slides rather than tissue samples however I am curious if it is normal that everything on the slide stains red. I realise that the collagen fibers can be easily detected in cross polarised and part polarised light ( yellow and orange = large fibers and green the thinner fibers and all are highly birefringent). While only the collagen changes colours in cross polarised light I am wondering if it is normal that everything on the slide picks up the stain to some extent and turns red (pink) when viewed in plane polarised light? This includes seed parts, plant lipids and ochre? Any thoughts or ideas? Thanks Regards Birgitta Stephenson Archaeological Microscopy Research Lab University of Queensland Birgitta Stephenson Pharmacist Advisor Veteran Affairs Pharmacy Advisory Centre Phone (07) 3223 8435 Fax (07) 3223 8651 __ IMPORTANT 1. Before opening any attachments, please check for viruses. 2. This e-mail (including any attachments) may contain confidential information for the intended recipient. If you are not the intended recipient, please contact the sender and delete all copies of this email. 3. Any views expressed in this e-mail are those of the sender and are not a statement of Australian Government Policy unless otherwise stated. 4. Electronic addresses published in this email are not conspicuous publications and DVA does not consent to the receipt of commercial electronic messages. 5. To unsubscribe from emails from the Department of Veterans' Affairs (DVA) please go to http://www.dva.gov.au/contact_us/Pages/feedback.aspx , and advise which mailing list you would like to unsubscribe from. 6. Finally, please do not remove this notice. -- Birgitta Stephenson bstep...@fastmail.fm -- http://www.fastmail.fm - Or how I learned to stop worrying and love email again ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
