[Histonet] Position Update - SW Chicago Suburbs
I had previously provided information on part time positions in the SW suburban location in Chicago. Currently, one of those positions has been upgraded to a full time position. If interested, please provide a CV and letter to me, and I will then forward. Thank you! Have a wonderful Holiday. Dr. Ruby ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] equipment and supply donations for start up non profit histolab in DC
I am usually the one giving stuff away and I now find myself in need of a handout. If anyone in histoland has any items they can get rid of please email me back so we can work out some details. I can use anything in terms of supplies, expendables, equipment. I have a microtome and water bath past that I have an empty room. Many thanks! Nick(Rocky) Madary, HT/HTL(ASCP)QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hard tissue microtome
Hi! could anyone recommend me a good microtome for hard tissue (specifically for bone tissue)? Thanks' in advance Hope you all have a good nativity and happy new year! silvina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 97, Issue 26
Miha, You need to lower down the cryostat at least -25 degree. You also need to spray your block the very minute before the cutting (not just one time spray on the OCT block). The thicker the section, the easier it can be cut. I also put my OCT blocks on the dry ice next to me, and transfer to the cryostat when I am ready to cut. Good Luck (I know it's not easy)! Madeleine Supervisor-Pathology (IPOX Histology) El Camino Hospital On Thu, Dec 22, 2011 at 10:00 AM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. out of office at noon today (marilyn.a.we...@kp.org) 2. TRAP Staining (Silvina Molinuevo) 3. Re: control block log (Richard Cartun) 4. Re: Histonet Digest, Vol 97, Issue 25 (naveeda arshad) 5. Re: Re: Histonet Digest, Vol 97, Issue 25 (Miha Tesar) 6. Position Update - SW Chicago Suburbs (Stephen G. Ruby) 7. equipment and supply donations for start up non profit histolab in DC (mad...@verizon.net) -- Message: 1 Date: Wed, 21 Dec 2011 10:01:33 -0800 From: marilyn.a.we...@kp.org Subject: [Histonet] out of office at noon today To: histonet@lists.utsouthwestern.edu Message-ID: of5129baae.0f3a19fc-on8825796d.00630526-8825796d.00630...@kp.org Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 12/20/2011 and will not return until 12/27/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. I will be in town for some of the time. Thank you. -- Message: 2 Date: Wed, 21 Dec 2011 11:07:15 -0800 (PST) From: Silvina Molinuevo silvinamolinu...@yahoo.com.ar Subject: [Histonet] TRAP Staining To: da...@uab.edu da...@uab.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1324494435.42985.yahoomail...@web113610.mail.gq1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Dear Dave, we use the next protocol with pretty good results. For diazotation reaction (Solution A): Dissolve 7mg Fast Garnet GBC base in 1ml of methyl or ethylenglygol. Add 1ml HCl 0.1N. Add drop by drop 2.07mg NO2Na. Keep T below 15ºC. Solution B:12.5mg/ml Naftol ASBI phosphate in distilled water For the enzymatic reaction: Disolve 75mg Sodium tartrate in 45 ml Citrate buffer pH 4.9. Pre-warm to 37ºC Add 2ml Solution A 0.5ml Solution B Incubate the slides about 1h at 37ºC. The most difficult thing is the diazotation reaction. You must have the stoichiometric relationship between Fast Garnet and Sodium nitrite. Sodium nitrite must be as pure as you can. You also have to retain the stoichiometry relation with chlorhidric acid to avoid reaction subproducts. Diazotation T must be not over 15ºC and you should use the diazotation product Best regards, silvina Dr María Silvina Molinuevo Grupo de Investigacion en Osteopatias y Metabolismo Mineral Departamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115 (1900)La Plata Argentina www.biol.unlp.edu.ar/giomm e-mail: silvina.molinu...@bigfoot.com -- Message: 3 Date: Wed, 21 Dec 2011 19:28:19 -0500 From: Richard Cartun rcar...@harthosp.org Subject: Re: [Histonet] control block log To: stephen.cla...@hcahealthcare.com, Histonet@lists.utsouthwestern.edu Message-ID: 4ef23353.7400.007...@harthosp.org Content-Type: text/plain; charset=US-ASCII I use FileMaker Pro software to keep track of our IHC controls. I created a control tissue file that includes the following: Control #, Case #, Tissue site, Diagnosis, DOS, Time in formalin, Processing date, Number of blocks submitted, and then a Comment field for the immunoreactivity for each antibody tested. Every file can then be searched by anyone of the data fields. For example, if we need a control for Parvovirus we would type in Parvovirus in the Diagnosis or Comment fields and then every control tissue tested positive (or negative) for Parvovirus would be identified. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
RE: [Histonet] Hard tissue microtome
Can you tell us more about the bone specimens you are wanting to cut? Jack Jack Ratliff Senior Histologist, BioMimetic Therapeutics, Inc. Chairman, Hard Tissue Committee - National Society for Histotechnology Date: Thu, 22 Dec 2011 10:25:35 -0800 From: silvinamolinu...@yahoo.com.ar To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hard tissue microtome Hi! could anyone recommend me a good microtome for hard tissue (specifically for bone tissue)? Thanks' in advance Hope you all have a good nativity and happy new year! silvina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Gram stain controls
Naveeda Arshad (where?) asks: Does any one has idea about how to make in house gram positive and negative control in your lab? What kind of tissue is suitable and and i need detail procedure for that? Usually a section of a ruptured appendix (easy enough to get in a hospital histology lab) will provide an abundance of suitable bacteria. A better solution is not to do a tissue Gram stain at all. You want to see bacteria - you really can't identify them in tissue sections. A simple tissue Giemsa or Diff-Quik II stain is both sensitive and specific for seeing bacteria of all kinds. (The ruptured appendix will work well as a control.) Pathologists are much too ready to order a stain that's of very dubious clinical value, particularly since tissue Gram stains usually stain Gram negative organisms rather weakly. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re:sectioning fat
Instead of using the cryo sray, use liquid nitrogen. Make a thick cotton swab, soak it in the liquid nitrogen, place it on the fat letting it turn white then discard the first section off then pick up the next one. This works really well. The cryo chamber needs to be really cold as well -25 works best. Let me know if you need more help. I have multiple tricks for cryosectioning. Mequita Praet, HTL(ASCP)SLS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Histonet Digest, Vol 97, Issue 25
I would not use any freezing aerosols if there is any suspicion of infection in tissue or cryochamber has been used for prior sectioning without being decontaminated. Depending on how fatty the tissue is you need to be in the region -28 to -35 degs C on the specimen, knife and anti-roll plate, for that you need a decent cryostat. Best regards Alan Bright Sent from my BlackBerry® wireless device -Original Message- From: Miha Tesar sauco...@yahoo.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Wed, 21 Dec 2011 23:07:26 To: naveeda arshadnaveedafa...@yahoo.ca; histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Reply-To: Miha Tesar sauco...@yahoo.com Subject: Re: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 Haloha! Does any one has any idea about how to cut the fat tissue in cryostat (frozen section). Temperature of the chambre/specimen and the thickness of the slice. I use the cryo spray but does not help me much! THX Miha and Mary C. 2 all From: naveeda arshad naveedafa...@yahoo.ca To: histonet@lists.utsouthwestern.edu Sent: Thursday, December 22, 2011 4:19 AM Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 Hi Does make in house gram Positive and negative control in your lab. What kind of tissue is suitable and and i need detail procedure for that.Thanks --- On Wed, 12/21/11, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: From: histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 97, Issue 25 To: histonet@lists.utsouthwestern.edu Received: Wednesday, December 21, 2011, 10:17 PM Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Control Slides (Rene J Buesa) 2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne) -- Message: 1 Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST) From: Rene J Buesa rjbu...@yahoo.com Subject: RE: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu, MargaretSherwood msherw...@partners.org Message-ID: 1324488987.20130.yahoomailclas...@web65716.mail.ac4.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases. René J. --- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: RE: [Histonet] Control Slides To: Rene J Buesa rjbu...@yahoo.com, histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 12:15 PM That is ideal Rene, but we are a research lab and don't run the same tissue all the time. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Wednesday, December 21, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. René J. --- On Wed, 12/21/11, Sherwood, Margaret msherw...@partners.org wrote: From: Sherwood, Margaret msherw...@partners.org Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list
[Histonet] RE. equipment and supply donations
Hey Rocky, We may have some stuff. Give me a call. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Control Slides
I've used http://www.americanmastertech.com/store/main.aspx?p=Categorybodyc=CV Only when I couldn't use what we had in the lab. Also some people might trade you if you have something they can use? Another thing I've noticed is occasionally vendors would request a control block for another client. I've always helped when I could so maybe you can ask for help from your vendors? Best of luck. Kim Sent from my iPhone On Dec 21, 2011, at 11:52 AM, Sherwood, Margaret msherw...@partners.org wrote: To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue Gram stain controls
But if you don't have a pathologist as compassionate as Dr Richmond and you are bound to do it. I would get microbiology to grow you a batch. Then plate/insert them into a section of lung. I prefer lung because it provides some background but not too much. This is just one way I've seen this done a long time ago. Good luck Kim Donadio Sent from my iPhone On Dec 22, 2011, at 2:10 PM, Bob Richmond rsrichm...@gmail.com wrote: Naveeda Arshad (where?) asks: Does any one has idea about how to make in house gram positive and negative control in your lab? What kind of tissue is suitable and and i need detail procedure for that? Usually a section of a ruptured appendix (easy enough to get in a hospital histology lab) will provide an abundance of suitable bacteria. A better solution is not to do a tissue Gram stain at all. You want to see bacteria - you really can't identify them in tissue sections. A simple tissue Giemsa or Diff-Quik II stain is both sensitive and specific for seeing bacteria of all kinds. (The ruptured appendix will work well as a control.) Pathologists are much too ready to order a stain that's of very dubious clinical value, particularly since tissue Gram stains usually stain Gram negative organisms rather weakly. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 97, Issue 26
Naveeda, You can use a piece of fresh placenta or lung and just add the (+) (-) gram bacteria (microbiology lab always have them in culture) to the tissues. Let the bugs infect the tissues for overnight @ room temperature or in a bacteria incubator (if you have one) @ 37c for overnight. I just add enough saline to the tissue and keep it moist. I chose placenta because it is easy to obtain (if there is a maternal ward, then plenty placenta after the delivery). Good Luck! Madeleine Supervisor-Pathology (IPOX Histology) El Camino Hospital 2500 Grant Road Mountain View, CA 94040 madelein...@elcaminohospital.org On Thu, Dec 22, 2011 at 10:54 AM, Madeleine Huey madeleineh...@gmail.com wrote: Miha, You need to lower down the cryostat at least -25 degree. You also need to spray your block the very minute before the cutting (not just one time spray on the OCT block). The thicker the section, the easier it can be cut. I also put my OCT blocks on the dry ice next to me, and transfer to the cryostat when I am ready to cut. Good Luck (I know it's not easy)! Madeleine Supervisor-Pathology (IPOX Histology) El Camino Hospital On Thu, Dec 22, 2011 at 10:00 AM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. out of office at noon today (marilyn.a.we...@kp.org) 2. TRAP Staining (Silvina Molinuevo) 3. Re: control block log (Richard Cartun) 4. Re: Histonet Digest, Vol 97, Issue 25 (naveeda arshad) 5. Re: Re: Histonet Digest, Vol 97, Issue 25 (Miha Tesar) 6. Position Update - SW Chicago Suburbs (Stephen G. Ruby) 7. equipment and supply donations for start up non profit histolab in DC (mad...@verizon.net) -- Message: 1 Date: Wed, 21 Dec 2011 10:01:33 -0800 From: marilyn.a.we...@kp.org Subject: [Histonet] out of office at noon today To: histonet@lists.utsouthwestern.edu Message-ID: of5129baae.0f3a19fc-on8825796d.00630526-8825796d.00630...@kp.org Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 12/20/2011 and will not return until 12/27/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. I will be in town for some of the time. Thank you. -- Message: 2 Date: Wed, 21 Dec 2011 11:07:15 -0800 (PST) From: Silvina Molinuevo silvinamolinu...@yahoo.com.ar Subject: [Histonet] TRAP Staining To: da...@uab.edu da...@uab.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1324494435.42985.yahoomail...@web113610.mail.gq1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Dear Dave, we use the next protocol with pretty good results. For diazotation reaction (Solution A): Dissolve 7mg Fast Garnet GBC base in 1ml of methyl or ethylenglygol. Add 1ml HCl 0.1N. Add drop by drop 2.07mg NO2Na. Keep T below 15ºC. Solution B:12.5mg/ml Naftol ASBI phosphate in distilled water For the enzymatic reaction: Disolve 75mg Sodium tartrate in 45 ml Citrate buffer pH 4.9. Pre-warm to 37ºC Add 2ml Solution A 0.5ml Solution B Incubate the slides about 1h at 37ºC. The most difficult thing is the diazotation reaction. You must have the stoichiometric relationship between Fast Garnet and Sodium nitrite. Sodium nitrite must be as pure as you can. You also have to retain the stoichiometry relation with chlorhidric acid to avoid reaction subproducts. Diazotation T must be not over 15ºC and you should use the diazotation product Best regards, silvina Dr María Silvina Molinuevo Grupo de Investigacion en Osteopatias y Metabolismo Mineral Departamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115 (1900)La Plata Argentina www.biol.unlp.edu.ar/giomm e-mail: silvina.molinu...@bigfoot.com -- Message: 3 Date: Wed, 21 Dec 2011 19:28:19 -0500 From: Richard Cartun rcar...@harthosp.org Subject: Re: [Histonet] control block log To: stephen.cla...@hcahealthcare.com, Histonet@lists.utsouthwestern.edu Message-ID: 4ef23353.7400.007...@harthosp.org Content-Type: text/plain; charset=US-ASCII I use FileMaker Pro software to keep track of our IHC controls. I
