[Histonet] re 2050 microtome
This could be as simple as moving the specimen holder forward. It may be racked right into the microtome and therefore show as stopped. Try moving the holder forward and see if the stopped light goes off. Regards steve weston lab manager Breathe-Well CRE UTAS-SOM Message: 9 Date: Sat, 15 Jun 2013 00:07:51 -0300 From: C.D.G. late...@montevideo.com.uy Subject: [Histonet] manual setup for Reichert-Jung 2050 needed To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: 201306150007510574.0040b...@smtp.montevideo.com.uy Content-Type: text/plain; charset=ISO-8859-1 Hi all: i received a Reichert-Jung microtome 2050 model. I need instructions for its operation. The electronic panel displays stop illuminated and I dont know how to continue, as other buttons seems not to operate. Any help of people who has worked or know to operate this motorized microtome will be appreciated. My kind regards, Carlos Defeo Histotechnologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cutting paraffin sections on a cryostat operated at room temperature? Nope.
Ever tried turning the handle of the cryostat, when it's at room temperature? Cryostats are tooled manufactured to operate at a low temperature. Since metal contracts at the low temperature, you'll find that you can't operate the microtome at the higher temperature. The handle will barely move. Sandy Harrison, HTL (ASCP) Histology Supervisor Minneapolis VAHCS 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Friday, June 14, 2013 3:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cutting paraffin sections...on a cryostat? Hi, all. A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts. Any thoughts? Kevin Johnson University of Miami Diabetes Research Institute ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin processing native sheep ACL
You might also consider using methyl salicylate instead of xylenes. Thanks to the help of Bob Skinner, I have achieved very nice results with native tendon. Generally speaking these MS steps will take a little longer, but you can monitor the progress very easily by watching for complete transparency of the tendon. You can then even develop a somewhat standardized protocol if you plan to process this type of tissue in the future. You even have a lot more flexibility with MS than xylenes as prolonged use in xylenes can make the tissue more hardened and brittle. Lastly, it is not generally recommended to put MS on the tissue processor, so I process to the final 100% EtOH, perform the MS exchanges by hand, transfer the tissues into a manual wax step to get rid of as much MS as possible and then finish with three (3) automated wax steps on the tissue processor. For my wax infiltration I use a 50:50 blend of TissuePrep from Fisher Scientific and EM400 from Leica. I then embed in 100% EM400. Best Regards, Jack Jack L RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology From: a.pr...@tissueregenix.com To: histonet@lists.utsouthwestern.edu Date: Fri, 14 Jun 2013 07:45:47 + Subject: Re: [Histonet] Paraffin processing native sheep ACL CC: Hi Liz, I inherited the following protocol for ACL samples. It works quite well, but times probably could be reduced - the optimization is on my to-do list. 70% Alcohol - 1 hour 90% alcohol - 1 hour 100% Alcohol -2 hours 100% alcohol - 3 hours 100% alcohol - 4 hours Xylene - 1.5 hours Xylene - 1.5 hours Xylene - 3 hours Wax - 3 hours Wax - 3 hours Wax - 4 hours I cut the sections at 8um so they hold together better. Takes a while for all the wrinkles to disappear when floating out on water-bath so be patient Hope this helps. Andrew Andrew Prior Histologist Tissue Regenix Group E-mail: a.pr...@tissueregenix.commailto:a.pr...@tissueregenix.com Website: www.tissueregenix.comhttp://www.tissueregenix.com/ -- Message: 3 Date: Wed, 12 Jun 2013 16:59:52 -0400 From: Elizabeth Ronan lizro...@umich.edumailto:lizro...@umich.edu Subject: [Histonet] Paraffin processing native sheep ACL To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Message-ID: 51b8e148.3020...@umich.edumailto:51b8e148.3020...@umich.edu Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello, I need to paraffin process native sheep anterior cruciate ligament (ACL) that has been fixed in 10% neutral buffered formalin for 7 days. I was wondering if anyone with more expertise on this subject could guide me with the best lengths of times in the alcohols, xylenes, and paraffin to fix ACL. I tried a 12 hour program but the sections crumbled in the middle and it appeared that the paraffin had not fully perfused the ligament. I have access to the following program, and can alter the lengths of the steps for as long as desired: Program: 70% 80% 95% 95% 100% 100% Xylene Xylene Paraffin Paraffin Paraffin Any advice is much appreciated. Thanks for your time, Liz The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Although we routinely screen for viruses, addressees should check this e-mail and any attachment for viruses. We make no warranty as to absence of viruses in this e-mail or any attachments. Registered Office: The Biocentre, Innovation Way, Heslington, York, YO10 5NY Registered No. 05969271 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Picro Sirius Red Stain
Hi All, Hope all are having a great Monday I am trying to do a Picro Sirius Red stain and have looked at many protocols that seem to not use a particular reagent that is in a manufacturers kit. I was hoping if someone could enlighten me as to why this reagent would be used and if someone is using it at what strength is the PhosoMolybdic Acid concentration. Your help will be greatly appreciated Kind Regards! John J Shelley Research Specialist, Histology Core Facility ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing Guinea Pig
Hello Histonet! I'm a long time reader first time poster. Does anyone have experience processing guinea pig tissues? I have been processing kidney and heart but it is consistently coming out mushy in the middle. The mouse tissue comes out fine even when processed on the same run. I had the tissue grossed in thinner (2.5mm) thinking that perhaps it was too thick but it didn't seem to help. Also, it has been fixed in 10%NBF for several days. I was just wondering if anyone else had similar problems with guinea pig? I appreciate in advance any advice or tips. Here is the protocol: Formalin 1hr 70% etOH 1hr 95% 1hr 100% 30min 100% 1hr 100% 1hr 100%1hr Clearify 1hr Clearify 1hr Clearify 1hr Paraffin 1hr paraffin 1hr All under pressure and heat only on the paraffin. Thanks Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Picro Sirius Red Stain
I do a Picro Sirius Red stain for collagen (got the protocol from Gayle Callis) and don't use Phosphomolybdic acid. My protocol is simple: one hour in PSR two rinses in acidified water rinse you can counterstain but my clients don't want a counterstain so I dehydrate, clear and coverslip. Boom, I'm done. The PSR stain is just Sirius Red F3B 0.5 gms and 500 ml of Sat. Picric Acid. Do not use a dye that is not CI 35780. The acidified water is 5ml Glacial Acetic Acid to 1L of DH2O Under polarized light the PSR stain is gorgeous! I love looking at the orange, yellow, pinks and green colors. Good thing because I do this stain all the time. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat Repair Service in San Diego
Good Morning, Can anyone recomend a good, reliable cryostat repair service in the San diego area? thanks Dusko ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Picro Sirius Red Stain
Dear John, I usually perform Picrosirius Red stain in cardiac tisse sections. I use phosphomolibic acid treatment in order to eliminate the cytoplasmic staining improving collagen/myoplasm contrast. Have a look at this paper: Picrosirius red staining of cardiac muscle following phosphomolybdic acid treatment Dolber PC, Spach MS Stain Technol. 1987 Jan;62(1):23-6. Good luck! Laura Hi All, Hope all are having a great Monday I am trying to do a Picro Sirius Red stain and have looked at many protocols that seem to not use a particular reagent that is in a manufacturers kit. I was hoping if someone could enlighten me as to why this reagent would be used and if someone is using it at what strength is the PhosoMolybdic Acid concentration. Your help will be greatly appreciated Kind Regards! John J Shelley Research Specialist, Histology Core Facility ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Laura Avogaro University of Trento Via delle Regole, 101 38123 Mattarello (TN) Italy Tel: +39 0461 283425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ACIS CALIBRATION SLIDE SET
Does anyone have an ACIS Calibration set they are not using and are willing to part with? One of my slides broke and I can't find a replacement anywhere. Thanks, Kari Breal Kari Breal, HT (ASCP) Histology Manager Alexian Brothers Health System ABMC-847-437-5500 ext. 5155 SAMC-847-843-2000 ext. 6818 kari.br...@alexian.netmailto:kari.br...@alexian.net CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing Guinea Pig
Heather, Looking at your protocol for processing the kidney and heart tissue I can't figure out why it is mushy especially if you are putting it in the cassettes so thin and it has had a chance to fix well before processing. In fact, looking at your protocol I might think that your tissue might be dried out and need to sit in cold icy water before sectioning. At any rate, I would rinse well in running water after fixing and skip the formalin on the processor. I'd start in 70% alcohol, maybe two changes and move on up to 80%, 2- 95%'s and 3-100%'s. I'm not familiar with the clearing agent that you use. I use Clear Rite 3 and never have a problem - 2 changes of Clear Rite 3 and 4 paraffins. I don't know how many cassettes you are processing but make sure there is adequate room for the reagents on the processor to move around the cassettes and that you have a good ratio of the reagents to the tissue and that the reagents are fresh. If you can increase the agitation in some of the dehydration steps it might help. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Parts for Sakura Tissue Tek 5 Embedding Center
Hello all you Histologists, Does anyone know of a used Histology equipment company that sells Sakura parts? We have a fairly new embedding center, (but past warranty), that needs a single heater unit. Sakura only sells the entire dispenser unit for nearly $8,000. I don't mean to offend any Sakura fans, but I don't think that's right. Thanks, Sandy Histology Necropsy Supervisor UW-Madison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology position
Hi Histonetters, I am moving back to Phoenix, Arizona and I am looking for a Histology position. I have over 17 years experience in the Histology field. I am willing to work full time and even part time. Please feel free to contact me at kecsl...@yahoo.com I hope to hear from you soon. Thank you for your time and consideration. Kristy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Blade Rationing
I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoor...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blade Rationing
That's penny pinching right there. I'd say fine, you tell me when to change blades and then put through the crap slides they get. Just avoid the calculi and staples at all costs... sometimes these number crunchers don't think. Curt -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Monday, June 17, 2013 2:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoor...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Blade Rationing
Sorry, I tell my techs a knife is cheaper than a complaint from the pathologists so, please don't abuse. H owever; don't hesitate to use what you need. As teh old saying oges for us A happy Patholgist is a happy life!! A dull is bad for sectioning and is dangerous overall. I can't imagine telling my Histologist to cut corners on knives and they know me well enough that they would think I had lost my mind!! So many other places to cut cost and not affect section quality. Pam Marcum Histology Supervisor UAMS - Original Message - From: Teresa Moore tmoor...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Monday, June 17, 2013 4:10:51 PM Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoor...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Blade Rationing
From a managers point of view, whoin my opinion that is a poor way to try to cut expenses. It will only lead to recuts and possible loss of important tissue. For the techs to understand the necessity to conserve is important but the tech needs to use their discretion as to when a blade needs changing. Sent from my iPhone On Jun 17, 2013, at 5:08 PM, Teresa Moore tmoor...@gmail.com wrote: I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoor...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT and HTL Job Descriptions
We are in the process of updating job descriptions and want to use this opportunity to see how other organizations are handling the difference in job descriptions between HT's and HTL's. Obviously if you have a different pay schedule for the two positions you need to clearly delineate the job descriptions and specify the increased responsibilities of staff that are HTL's. I am interested in how other institutions have handled this issue. In the past we have had the same job description for HT and HTL and have also not had a difference in pay schedules. I know that some places do not separate the two positions. Any information that you could share would be helpful as we look into this project. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HT and HTL Job Descriptions
St. Anthony Hospital, located in Oklahoma City, Oklahoma currently has an excellent opportunities for experienced Histologic Technicians. To work for our organization requires Certification as an HT or HLT by the American Society of Clinical Pathologists (ASCP) - or - other nationally recognized certifying agency acceptable to the Laboratory Director - or - experience acceptable to the Laboratory Director. Two years of previous histology experience required. Outstanding benefits package, competitive pay and generous paid time off. For consideration, please apply online at www.saintsok.com, Ad # 17929, or contact Renee Watley for additional information at (405) 272-6016; renee_wat...@ssmhc.com. Cynthia L. Brundige SSM Health Care of Oklahoma Regional Vice President-Human Resources 1000 N. Lee Street, P.O. Box 205 Oklahoma City, Oklahoma 73101 cynthia_brund...@ssmhc.com -Original Message- This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HT and HTL Job Descriptions
We have Histotechnologst 1, 2, 3, and Lead, Supervisor: We don't have a requirement that they have their HT or HTL certification, but do need specific experience in a histology lab. All require bachelor's degree with educational qualifications to sit for HT or HTL exam. Most have their HT or HTL. A few new people do not...yet. 1 - entry level bench tech, education equiv to one year experience ,ie some lab work. 2 - having one year histo lab experience 3 - having 4 years experience (Senior tech), Lead - having 5 years experience including all aspects of the lab. Supervisor, 10 years experience, general supervision and administration. Levels 1 and 2 are general bench techs rotating to all areas of the lab Level 3 is Senior tech who can be made responsible for overseeing one are of the lab, not to work there all the time, but be responsible for making sure everything is ship shape and people have what they need, do validations of stains and equipment. They also can take charge at the times a Lead or Supervisor is not around (ie, early AM or late PM) Lead Tech does more admin work (in addition to some bench work), troubleshooting, oversees procedure validation, workflow scheduling on the fly, generally keeps the lab running. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, June 17, 2013 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT and HTL Job Descriptions We are in the process of updating job descriptions and want to use this opportunity to see how other organizations are handling the difference in job descriptions between HT's and HTL's. Obviously if you have a different pay schedule for the two positions you need to clearly delineate the job descriptions and specify the increased responsibilities of staff that are HTL's. I am interested in how other institutions have handled this issue. In the past we have had the same job description for HT and HTL and have also not had a difference in pay schedules. I know that some places do not separate the two positions. Any information that you could share would be helpful as we look into this project. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
