[Histonet] 2014 NSH Teleconferences/Webinars

2013-11-04 Thread Lee Peggy Wenk
Promotion about NSH teleconferences for next year – if not interested, delete 
now.

FYI – the 2014 NSH webinar schedule is now available, and so is signing up. 
http://www.nsh.org/content/2014-nsh-laboratory-webinar-series-registration-now-open

If this link doesn’t work, go to NSH webpage www.nsh.org
Part way down on the left it says 2014 NSH Laboratory Webinar Series 
Registration Now Open
Just click on that.

One each month, usually 4th Wed of month (unless NSH Symposium or a major 
holiday interferes), from 1-2 pm Eastern Time.

Can have as many people attend as you want, and each gets 1 hour CE. Your lab 
also gets a link afterwards, and for those who couldn’t attend (working, 
off-shift, vacation, etc.), they can participate up to 2 years later, and still 
get CE.

$125 for each month, $1350 for all 12 months if you sign up by 1/22/14 (savings 
of $150, or better than buy 11, get 1 free).

IHC, histology, autopsy, safety ergonomics. 

Peggy A. Wenk, HTL(ASCP)SLS
NSH Webinar Coordinator
(No, I don’t get any money for doing this job or advertising it – I’m a 
volunteer)
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RE: [Histonet] Billing question

2013-11-04 Thread Gray, Ed
Professional only 


Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU 
Healthcare l eg...@wvuhealthcare.com
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Friday, November 01, 2013 3:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Billing question

I have a billing question that I would sure like to hear what others are doing, 
please.  In AP billing, when billing for CPT #88321 Consultation and report on 
referred slides prepared elsewhere  do you bill both a technical and 
professional component, or professional only?

Any input would be appreciated.  Sincerely, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
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[Histonet] basement membranes

2013-11-04 Thread Edwards, Richard E.

Best  technique, tinctorial or otherwise of identifying, with a  view to  
measuring their width, many  thanks.

 
Richard  Edwards


  Leicester University


 U.K.
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[Histonet] Problems with HE Bleeding

2013-11-04 Thread Anna Coffey
Hello Histonetters,

We have recently noticed some bleeding of our HE stained tissues and I was
curious to hear if anyone has had problems with this before.  There will
just be a faint halo of stain around the tissue.  This seems to be
happening inconsistently (various tissues, even differences within the same
stained batch) and has now occurred with both paraffin-embedded and frozen
tissue, so with 2 different programs in our autostainer.  If you've seen
this in your lab before, were you able to isolate the cause and fix it?
 I'd be very appreciative of any advice you may have about what we can do
to fix this problem.

Thanks,
Anna

-- 
Anna Coffey
Senior Histology Technician
Department of Oncology
Histopathology and Tissue Shared Resource
LR-10 Pre-Clinical Sciences Building
Lombardi Comprehensive Cancer Center
Georgetown University
202-687-7890
ah...@georgetown.edu
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[Histonet] Aspergillus tissue blocks for controls

2013-11-04 Thread Vickroy, Jim
We are in desperate needs of obtaining GMS controls.   In the old days we had 
all the fungal specimens we needed.  Today it is getting hard to find these 
tissues or controls.   Does anyone have any idea where we can get tissue blocks 
with Aspergillus or does anyone have any other suggestions about GMS controls.

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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RE: [Histonet] Problems with HE Bleeding

2013-11-04 Thread Laurie Colbert
You probably have water and/or alcohol contamination in your xylenes.  Make 
sure  your dehydrating alcohols after the eosin are really clean.

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Coffey
Sent: Monday, November 04, 2013 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Problems with HE Bleeding

Hello Histonetters,

We have recently noticed some bleeding of our HE stained tissues and I was 
curious to hear if anyone has had problems with this before.  There will just 
be a faint halo of stain around the tissue.  This seems to be happening 
inconsistently (various tissues, even differences within the same stained 
batch) and has now occurred with both paraffin-embedded and frozen tissue, so 
with 2 different programs in our autostainer.  If you've seen this in your lab 
before, were you able to isolate the cause and fix it?
 I'd be very appreciative of any advice you may have about what we can do to 
fix this problem.

Thanks,
Anna

--
Anna Coffey
Senior Histology Technician
Department of Oncology
Histopathology and Tissue Shared Resource
LR-10 Pre-Clinical Sciences Building
Lombardi Comprehensive Cancer Center
Georgetown University
202-687-7890
ah...@georgetown.edu
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Re: [Histonet] basement membranes

2013-11-04 Thread Lee Peggy Wenk

Electron Microscope.

Even if you did a periodic acid-methenamine silver stain (PASM, Jones), 
which in my opinion is the best histology stain, since it is a silver stain, 
you can get different thicknesses of basement membrane (bm) by leaving it in 
longer or shorter than is optimal for that patient's bm thickness.


Peggy A. Wenk, HTL(ASCP)SLS

-Original Message- 
From: Edwards, Richard E.

Sent: Monday, November 04, 2013 9:39 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] basement membranes


Best  technique, tinctorial or otherwise of identifying, with a  view to 
measuring their width, many  thanks.



Richard  Edwards



 Leicester University



U.K.

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[Histonet] GMS fungus controls

2013-11-04 Thread Davis, Cassie
Jim,

We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to 
find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888

Cassandra Davis
cda...@che-east.org
302-575-8095




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[Histonet] (no subject)

2013-11-04 Thread Davis, Cassie
Jim,

We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to 
find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888

Cassandra Davis
cda...@che-east.org
302-575-8095




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not the intended recipient, please delete this message, and
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Re: [Histonet] Aspergillus tissue blocks for controls

2013-11-04 Thread Patrick Laurie
Hi Jim,

I have found a couple of ways.  First, if you are fortunate to have a micro
department nearby, they can make a very nice one for you (a Histotip in
Sakura's Histologic).  Another method, which doesn't work the best,  can be
moldy orange peel.  And the easiest, you can create your own (moldy
sausage) or even take some human tissue (fresh lung works the best) and
leave it with some moldy meat, then you can get a great one.

Good luck!


On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim vickroy@mhsil.com wrote:

 We are in desperate needs of obtaining GMS controls.   In the old days we
 had all the fungal specimens we needed.  Today it is getting hard to find
 these tissues or controls.   Does anyone have any idea where we can get
 tissue blocks with Aspergillus or does anyone have any other suggestions
 about GMS controls.

 James Vickroy BS, HT(ASCP)

 Surgical  and Autopsy Pathology Technical Supervisor
 Memorial Medical Center
 217-788-4046


 
 This message (including any attachments) contains confidential information
 intended for a specific individual and purpose, and is protected by law. If
 you are not the intended recipient, you should delete this message. Any
 disclosure, copying, or distribution of this message, or the taking of any
 action based on it, is strictly prohibited.
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-- 

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869
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[Histonet] Problems with HE Bleeding

2013-11-04 Thread P.E. Visser
Dear anna 

I had while working for Baker chemicals, a customer in Germany who had a
similar problem there HE turned purple. the red disappeared. the
environment around the section contained little red droplets (only visible
under high magnification). after some time (because at first they clamed
noting was changed) it became clear  that they skipped ethanol 100 % and
just used the cheaper 96% instead. when evaluated they tested 3 slides and
that worked. but after 300 - 500 slides the xylene was saturated with water.
eosin being soluble in water migrates from the section to the water emulate
in the xylene. 

so I would test the ethanol you use. 3 times 100% is in my view a must.

 

I also on time in Scotland was confronted with xylene that was sold as non
recycled, but in fact it was. and sadly the supplier used an open container
to speed up cooling down. the rain was the source of the water. it lead to
several problems and took a long time before the we could find the source of
these problems. 

 

wish you success with solving yours.

regards piet

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[Histonet] AUTO: Bruce Palmatier is out of the office (returning 11/08/2013)

2013-11-04 Thread Bruce_Palmatier

I am out of the office until 11/08/2013.

I will be out of the office from Nov 4- Nov 8. If you have a question about
an order or need assistance placing an order, or if the matter is urgent,
please call 877-881-1192 between 8am and 8pm and a VWR Healthcare Service
associate will assist you. For other matters, I will respond to emails
within 24 hours. If you require pricing information, quotes, product
information, or other assistance, Tim Rafferty is the new VWR Healthcare
Sales Rep who's taken my place. He can be reached at
timothy_raffe...@vwr.com or (717) 668-9045.

Thank You,

Bruce Palmatier
Market Portfolio Manager
VWR Healthcare
bruce_palmat...@vwr.com
mobile: 484.319.5563
fax: 484-881-7307

Customer Service:  877.881.1192
Fax: 484.881.6486
Customer Service email: healthcareserv...@vwr.com


Note: This is an automated response to your message  Histonet Digest, Vol
120, Issue 5 sent on 11/4/2013 1:06:11 PM.

This is the only notification you will receive while this person is away.


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Re: [Histonet] Aspergillus tissue blocks for controls

2013-11-04 Thread Hans B Snyder
We have tried the moldy peel also but the pathologists did not like this
and would not accept any controls of this form.  I don't blame them they
want to see it naturally occurring.

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com ha...@histologistics.com


On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie foreig...@gmail.com wrote:

 Hi Jim,

 I have found a couple of ways.  First, if you are fortunate to have a micro
 department nearby, they can make a very nice one for you (a Histotip in
 Sakura's Histologic).  Another method, which doesn't work the best,  can be
 moldy orange peel.  And the easiest, you can create your own (moldy
 sausage) or even take some human tissue (fresh lung works the best) and
 leave it with some moldy meat, then you can get a great one.

 Good luck!


 On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim vickroy@mhsil.com
 wrote:

  We are in desperate needs of obtaining GMS controls.   In the old days we
  had all the fungal specimens we needed.  Today it is getting hard to find
  these tissues or controls.   Does anyone have any idea where we can get
  tissue blocks with Aspergillus or does anyone have any other suggestions
  about GMS controls.
 
  James Vickroy BS, HT(ASCP)
 
  Surgical  and Autopsy Pathology Technical Supervisor
  Memorial Medical Center
  217-788-4046
 
 
  
  This message (including any attachments) contains confidential
 information
  intended for a specific individual and purpose, and is protected by law.
 If
  you are not the intended recipient, you should delete this message. Any
  disclosure, copying, or distribution of this message, or the taking of
 any
  action based on it, is strictly prohibited.
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 --

 Patrick Laurie(HT)ASCP QIHC

 Histology Manager

 Celligent Diagnostics, LLC

 101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

 Work: 704-970-3300  Cell: 704-266-0869
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RE: [Histonet] Aspergillus tissue blocks for controls

2013-11-04 Thread Diana McCaig
As well we have used mushroom and other moldy food products but it only 
demonstrates the mold and not tissue elements.  But you can mince tissue and 
mix it with the fungus or mold so it one big segment and this gave more 
desirable results.

Diana 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder
Sent: November-04-13 4:47 PM
To: Patrick Laurie
Cc: histonet@lists.utsouthwestern.edu; Vickroy, Jim
Subject: Re: [Histonet] Aspergillus tissue blocks for controls

We have tried the moldy peel also but the pathologists did not like this and 
would not accept any controls of this form.  I don't blame them they want to 
see it naturally occurring.

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com ha...@histologistics.com


On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie foreig...@gmail.com wrote:

 Hi Jim,

 I have found a couple of ways.  First, if you are fortunate to have a 
 micro department nearby, they can make a very nice one for you (a 
 Histotip in Sakura's Histologic).  Another method, which doesn't work 
 the best,  can be moldy orange peel.  And the easiest, you can create 
 your own (moldy
 sausage) or even take some human tissue (fresh lung works the best) 
 and leave it with some moldy meat, then you can get a great one.

 Good luck!


 On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim vickroy@mhsil.com
 wrote:

  We are in desperate needs of obtaining GMS controls.   In the old days we
  had all the fungal specimens we needed.  Today it is getting hard to find
  these tissues or controls.   Does anyone have any idea where we can get
  tissue blocks with Aspergillus or does anyone have any other 
  suggestions about GMS controls.
 
  James Vickroy BS, HT(ASCP)
 
  Surgical  and Autopsy Pathology Technical Supervisor Memorial 
  Medical Center
  217-788-4046
 
 
  
  This message (including any attachments) contains confidential
 information
  intended for a specific individual and purpose, and is protected by law.
 If
  you are not the intended recipient, you should delete this message. 
  Any disclosure, copying, or distribution of this message, or the 
  taking of
 any
  action based on it, is strictly prohibited.
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 --

 Patrick Laurie(HT)ASCP QIHC

 Histology Manager

 Celligent Diagnostics, LLC

 101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

 Work: 704-970-3300  Cell: 704-266-0869 
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