[Histonet] John Caggiano’s passing
Dear HistoPeeps: I have some sad news to share with the histology/pathology community. Joe Caggiano informed me Monday that his brother John passed away this last Sunday night. John Caggiano, founder, President and CEO of Poly Scientific R & D Corp, died quietly in his sleep after a long illness. John and Poly Scientific were early and longtime supporters of NSH, (National Society for Histotechnologists). He was always supportive, generous, and it was a pleasure to spend time with him. John was a great friend and he will be greatly missed. RIP John. Regards, Akemi Allison-Tacha, BS, HT/HTL (ASCP) Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Faded H tissue section
Hi Tony: I actually gave that tip to Lee back in 1979 when he came to OHSU to give a seminar to the Oregon Histology Society. I found out that when I was doing GMA and the pathologists didn’t like Gills Hematoxylin. They loved my PAS on GMA so I tried using 1% Periodic Acid before using Harris Hematoxylin for H’s on GMA and it turned out beautifully. Guess he shared my tip with the rest of our society. He and I became closer se friends and we shared several tips, including his Movat’s tips which he didn’t publish, but I shared them in Frieda’s 4th Ed. Best, Akemi Allison-Tacha, BS, HT/HTL (ASCP) Sent from my iPhone > On Nov 9, 2023, at 6:11 PM, jayalakshmy p.s via Histonet > wrote: > > Thanks, I'll check it out. > >> On Fri, Nov 10, 2023, 6:58 AM Tony Henwood wrote: >> >> You could treat the decoverslipped section in 1% periodic acid (same as >> used in the PAS technique) for 30 minutes prior to H staining. This >> might improve the H staining. >> >> >> >> I believe Lee Luna suggested this but for the life of me, I can’t find the >> reference! >> >> >> >> Regards, >> >> >> >> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) >> >> Principal Scientist, the Children’s Hospital at Westmead (Retired) >> >> Adjunct Fellow, School of Medicine, University of Western Sydney. >> >> >> >> *From: *jayalakshmy p.s via Histonet >> *Sent: *Friday, 10 November 2023 3:43 AM >> *To: *histonet@lists.utsouthwestern.edu >> *Subject: *[Histonet] Faded H tissue section >> >> >> >> Hello, >> I would like to know how to effectively restain a faded H tissue section. >> The color becomes dull when re stained. Somebody please advise. >> Prof. Jayalakshmy. P S >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] [External] Re: Coffee at the desk
15:4-Observing Standard Precautions Occupational Safety and Health Administration (OSHA) Can a health care worker drink coffee in a laboratory where blood/body fluids/tissue tests are performed? Why or Why not? No, federal law prohibits eating and drinking in any area that can be potentially contaminated by blood or other body fluids. Regards, Akemi Allison-Tacha, BS, HT/HTL ASCP President/Consultant Phoenix Laboratory Consulting Sent from my iPhone On Jun 2, 2023, at 12:37 PM, Bitting, Angela K. via Histonet wrote: Oh, for the days when one could tuck a 32 oz. Slurpy inconspicuously behind the VIP without fear of admonishment. -Original Message- From: Jay Lundgren via Histonet Sent: Friday, June 2, 2023 3:34 PM To: Piche, Jessica Cc: Histonet@lists.utsouthwestern.edu Subject: [External] Re: [Histonet] Coffee at the desk External: Be careful with links and files. If suspicious, alert the Information Security Office. Questions? Call 570-271-8092 Next thing you're going to be telling me it's not OK to embed with a 1 foot tall bunsen burner open flame and a Coke on the cold tray! On Fri, Jun 2, 2023 at 7:46 AM Piche, Jessica via Histonet < histonet@lists.utsouthwestern.edu> wrote: It also doesn't set a good impression for everyone else in the lab since they can't have a coffee at their stations. If it could be done, I wouldn't want everyone else's coffees on my desk either, but I'd have to because I'd want to make it fair to everyone. If only it was safe for all of us Have a great weekend everyone! Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 From: McLaughlin, Stacy L. via Histonet ; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Coffee at the desk [EXTERNAL MSG] It is not allowed. The area you want food/drink has to be completely separate- floor to ceiling walls and a door. Stacy McLaughlin, HT(ASCP) QLScm Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 Office: (413)582-2019 Lab: (413)582-2179 smclaughl...@cooleydickinson.org -Original Message- From: Hannen, Valerie via Histonet Sent: Thursday, June 1, 2023 3:40 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coffee at the desk External Email - Use Caution Is there any regulation/ reason why a Histology supervisor whose desk is in the department and the desk area is taped off and designated as a "clean area ( meaning no chemicals or specimens cross the taped off area) can't have a closed coffee cup at their desk?? We are having a debate in our Lab and I wanted to get a consensus. Thanks in Advance, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com> http://secur/ e-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr 3g Tc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__w KY CLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_ 5X UvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9 IZ IGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuY q8 6VfPp5oSQ8Q%2Fhttp%253A%252F%252Fwww.parrishmed.com=05%7C01%7Ca kb itting%40geisinger.edu%7C4f92290a707e4df1d87108db63a05f02%7C37d46c56 7c 664402a16055c2313b910d%7C0%7C0%7C638213312668777202%7CUnknown%7CTWFp bG Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn 0% 3D%7C3000%7C%7C%7C=%2BQknLpXaFEZ0ilOK2JVWdUzUwrSmCapdNh1SOTmkk n0 %3D=0 < http://secur/ e-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr 3g Tc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__w KY CLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_ 5X UvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9 IZ IGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuY q8 6VfPp5oSQ8Q%2Fhttp%253A%252F%252Fwww.parrishmed.com=05%7C01%7Ca kb itting%40geisinger.edu%7C4f92290a707e4df1d87108db63a05f02%7C37d46c56 7c 664402a16055c2313b910d%7C0%7C0%7C638213312668777202%7CUnknown%7CTWFp bG Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1ha
Re: [Histonet] Alcian blue van gieson double stain
Just curious, why not do a Movat’s Pentachome? It gives you everything you are looking for and more. Best regards, Akemi Allison-Tacha, BS, Ht, HTL akemiat3...@gmail.com Sent from my iPhone > On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet > wrote: > > Hi, > if they are wanting to stain elastic, Alcian blue, and Van Gieson, I > recommend doing the Verhoff elastic stain first, then the Alcian blue, and > then the Van Gieson. > Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N > Blue Lake DriveNorman, OK 73069PH > 405-759-3953http://www.excaliburpathology.com > > A sharp knife is nothing without a sharp eye. - Klingon Proverb > >On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet > wrote: > > I am working with a researcher and they saw an article where someone > performed an Alcian Blue and elastic van Gieson double stain. If anyone > has done this before can you provide your protocol. I am trying to figure > out the best way to perform this process to get the best staining results. > Here are my questions/thoughts > > 1. Is it better to run the Alcian blue as usual and then perform the van > gieson stain? > 2. Will the differentiating solution in the van gieson stain affect the > alcian blue staining in any way? > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Validation of new G2 Coverslipper
Good Morning Histo Peeps: We just purchased a new Sakura G2 Coverslipper for our IHC Lab. The engineer will be coming Monday to make sure all adjustments are in alignment and fine tune anything which might have gotten out of alignment due to shipping. I have never validated a new Coverslipper in any of the labs I have managed. I searched the internet and was not able to find any information or instructions pertaining to Coverslipper Validation. I am of course going to run several test slides prior to running any actual cases on it. I will instruct my staff to date and provide test information on the slides to accompany the paperwork Sakura provides for the set-up of the equipment, but I am not sure that will be sufficient. Since we our a research facility, my IHC Director does not feel we need to validate our new Coverslipper to comply to GLP. I just want to make sure for my own assurance. I would greatly appreciate any guidelines for validation you may want to share with me. Best Regards, Akemi Eileen (Akemi) Allison, BS, HT/HTL (ASCP) IHC Manager HistoTox Labs, Inc. 2108 55th St. Suite 120 Boulder, CO 80301 Cell: (408) 335-9994 Office: 303-633-5401 Email: ealli...@histotoxlabs.com <mailto:ealli...@histotoxlabs.com> www.histotoxlabs.com <http://www.histotoxlabs.com/> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GLP lot# documentation
Hello Histo Land: I am the new IHC manager for a research lab which works primarily with animal tissue and we are moving towards GLP compliance for our department. We have an extremely large client base which consists of Big Pharma, private research companies, universities, etc… We are in the process of updating our SOW and SOP forms, Stain line maintenance forms, solution stainer change logs, etc… I have a question regarding documenting lot #’s for our hematoxylin counterstain protocol for IHC's. Do any of you who are GLP compliant document lot numbers for your alcohols and xylenes? Our director and QA officer would like to incorporate documenting lot numbers for alcohols and xylenes. Our histology department who is GLP certified only documents their lot numbers for Hematoxylin, Eosin, Bluing solution and High Def solutions for their H stain line. They currently do not document lot numbers for alcohols and xylenes. I have primarily worked for Clinical pathology labs, who did not document lot numbers for alcohols or xylenes. Please let me know if you do. Thanks, Akemi Eileen (Akemi) Allison, BS, HT/HTL (ASCP) Manager – IHC Department HistoTox Labs, Inc. 2108 55th St. Suite 120 Boulder, CO 80301 Cell: 408-335-9994 www.histotoxlabs.com <https://histotoxlabs.com/> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bx Processing run
Good morning Histopeeps: I am reaching out to you this morning to ask for your processing protocols for your biopsies and routines. Our department has two 25 year old VIP Tissue processors. Prior to my arrival, the department was only using one processor at a time and running the biopsies and routine tissues together. They were also only rotating solutions once a week even though there were close to 300 cassettes a day. With that being said, we are now using both tissue processors and separating biopsies from routines and we rotate all solutions daily. The specimens are put on the machines at 5:00 PM. This is not an option. Our biopsies finish at 5:00 AM and the routines finish at 6:30 AM. I would like to have our biopsies finish by 3:00 AM and the routines finish by 5:00 AM. Naturally, I do not want to compromise the quality of our tissue samples. I would appreciate you sharing your processing protocols with me which would include solutions and time in each station. Thank you in advance for your information. Akemi Allison, BS, HT/HTL (ASCP) Histology Supervisor UMC El Paso, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica's Spectra w/ coverslipper
Good morning Histopeeps: We will be receiving our new Spectra H Stainer w/ cover slipper the week of May 4th. This instrument was in negotiations for purchase prior to me coming on board. I have worked with the older models of Leica’s H Stainers, but am unfamiliar with this one. Could any of you who has this unit be willing to share your thoughts with me. It’s been several years since I have validated a new H stainer. Do I need to validate the instrument along with validating and optimizing the H stains? Thank you in advance for your information. Akemi Allison, BS, HTL/HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histologists assisting with transplants
Good morning histoland! Just curious, this is definitely a 1st for me. Received a call this morning from my histotech who is on call this week that she was called in at 3:30 AM to assist one our pathologists with a liver transplant. In all my years working in this field, histologists have never had anything to do with transplants. Have any of you out there had anything to do with transplants? Akemi Allison, BS, HTL Histology Supervisor UMC El Paso, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HistoQIP/PIP
Hello Histopeeps: Can someone tell me what year HistoQip start with NSH/CAP and PIP with CAP. Thank you! Akemi Allison, BS, HT/HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech openings in El Paso, TX
Happy Friday Histopeeps! Any Histotechs want to move to El Paso, TX? El Paso has a lot to offer! We have one current opening and another coming up in the next month. We are losing one of our best tech’s to FL. Her husband is a pathologist who is going to do his GI fellowship in Miami. Our lab is getting a Leica Upgrade starting in March! A Ceribro system, 2 new Bonds, a new H Stainer with Cover Slipper, and Cassette and Slide Printer. Email me and I will provide all information. Akemi Allison, BS, HT/HTL Histology Supervisor UMC El Paso, TX Any Histotech's want to move to El Paso? 2 openings coming up in my lab! Any Histotech's want to move to El Paso? 2 openings coming up in my lab! Any Histotech's want to move to El Paso? 2 openings coming up in my lab! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue disposal
Good morning Histopeeps! After reviewing our Policy Procedure Manual, our manager is questioning the bags we are using for disposing our tissues after diagnosis is complete. We are separating the tissue from the formalin by straining the excess formalin and neutralizing it. We then put the tissue into “Large Biohazard Bags” and the EVS staff picks it up and disposes it. My manager is wondering if the “Biohazard Bags" are appropriate. Should the bags state “Tissue” on the them? Are there any special disposal bags available? While working for a GI Lab in Monterey, CA we put all our bottle and tissue in "Biohazard Bags" and a Hazardous Waste company picked up the waste products from our endoscopy department along with ours. Any comments and suggestions would be greatly appreciated. Thank you in advance, Akemi Allison, BS, HT/HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel
Went on line to get MSDS regarding HistoGel. Here’s the link. https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf <https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf> Happy eating from a microwave which has been used at MD Anderson for HistGel! Best regards, Akemi > On Dec 12, 2019, at 5:18 AM, Kathleen S Cormier wrote: > > Can I share that we had a pathologist (back in the day) use our lab > microwave to re-heat his coffee? It had Schiff’s reagent stains all over the > inside of it. Some people really don’t have any common sense… > > > Kathy Cormier > Core Leader > Hope Babette Tang Histology Facility > Koch Institute for Integrative Cancer Research at MIT > 500 Main Street, 76-182 > Cambridge, MA 02139 > 617-258-8183 > corm...@mit.edu <mailto:corm...@mit.edu> > https://ki.mit.edu/sbc/histology <https://ki.mit.edu/sbc/histology> > > > > > > > > > >> On Dec 12, 2019, at 7:12 AM, Eileen Akemi Allison via Histonet >> > <mailto:histonet@lists.utsouthwestern.edu>> wrote: >> >> Good morning histopeeps: >> >> We recently brought on an Ophthalmology Pathologist from MD Anderson and >> they use histogel for orienting their thin eye specimens and need a >> microwave to heat it up. We do not have a microwave in the lab for their >> use but I had ordered one for them. I just found out they used the >> microwave in our lab lounge to heat up histonet which was opened and being >> stored in our gross area refrigerator. >> >> Do any of you know the CAP regulation against use of laboratory reagents >> being used in a microwave being used for food consumption? I ended up >> removing the microwave they used and put it in the histology lab. Our >> Director wants me to write a formal letter to submit to him. >> >> Thank you in advance for your impute! >> >> Akemi Allison, BS, HT/HTL (ASCP) >> >> >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu <mailto:Histonet@lists.utsouthwestern.edu> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] guidelines for using a microwave designated for food, but used to heat up histogel
Good morning histopeeps: We recently brought on an Ophthalmology Pathologist from MD Anderson and they use histogel for orienting their thin eye specimens and need a microwave to heat it up. We do not have a microwave in the lab for their use but I had ordered one for them. I just found out they used the microwave in our lab lounge to heat up histonet which was opened and being stored in our gross area refrigerator. Do any of you know the CAP regulation against use of laboratory reagents being used in a microwave being used for food consumption? I ended up removing the microwave they used and put it in the histology lab. Our Director wants me to write a formal letter to submit to him. Thank you in advance for your impute! Akemi Allison, BS, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Questions on how to operate a ThermoFisher Lab Vision autostainer 3602D
Ask Biocare or Dako. Unbeknownst to most of the public, it is the same exact unit they had years ago. Lab Vision made all 3 units. Their brand, Biocare and Dako. Lab Vision just put different branding on the various units when they were sold. Akemi Allison-Tacha, BS, HT/HTL (ASCP) Former Director and Operations Manager at Histology at Biocare > On Oct 24, 2019, at 12:49 PM, Bummer, Katherine via Histonet > wrote: > > Hello Histonetters, > > My company has acquired a refurbished ThermoFisher Lab Vision autostainer > 3602D and I have questions on how to operate. It is no longer supported by > ThermoFisher so I am unable to get tech support through them. Is there > anyone who is familiar and available/willing to answer a few questions? > > Thank you in advance. > > Kate > > Katherine Bummer > Nektar Therapeutics > 455 Mission Bay Boulevard South > San Francisco, CA 94158 > k.bum...@nektar.com<mailto:k.bum...@nektar.com> > 415.482.5716 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hints for cutting fatty FS
A while back on histonet a few people gave some tricks to cutting fatty tissue on histonet. Someone mentioned using 50% alcohol I believe. We had some extremely fatty LN’s yesterday. The pathologist cut away most of the fat, but the tech still had a hard time, so I got called in to help the tech. I was able to get sufficient sections, but they weren’t optimal. Could any of you share your secrets please. Thank you, Akemi Allison, BS, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cutting fatty FS
Good Morning Histoland, A while back on histonet a few people gave some tricks to cutting fatty FS tissue on histonet. Someone mentioned using 50% alcohol I believe. We had some extremely fatty FS LN’s yesterday. The pathologist cut away most of the fat, but the tech still had a hard time, so I got called in to help the tech. I was able to get sufficient sections, but they weren’t optimal. Could any of you share your secrets please. Thank you, Akemi Allison, BS, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo techs assisting on BM Bx's
Good Morning Histo Peeps: Back from a great trip to NSH NOLA and hit the ground running yesterday. I am faced with a department coverage issue which impacts our TAT. I am curious how many facilities have their histologists assist with BM Bx’s. I have never in my 50 years as a histologist assisted with BM Bx’s. That responsibility was under the hematology department responsibilities. We have 4 FT Histo tech’s and 1 PNR in our department. Yesterday we had 2 histologists pulled away from bench duties to assist with BM Bx’s, and then another histo tech was called to go to the main hospital to cut FS’s, which is 2 blokes away. One of the tech’s was gone for 3 hrs because the pathologist performing the BM was extremely slow. That left 1 tech and myself to cover bench duties. Keep in mind this is a county hospital with limited funding and a freeze on staffing. Thank you in advance for your input. Akemi Akemi Allison, BS, HT/HTL Histology Supervisor UMC El Paso, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Disposal of contaminated gloves, paper towels etc... after grossing
Good morning Histo Peeps: I am curious as to your laboratory/hospital policies on how to dispose of contaminated gloves, paper towels etc… after the PA/pathologists gross a specimen. Often times we receive fresh tissue in the lab and we do not know if the patient has TB, Hepatitis, HIV, or any other infectious disease. I was always taught that to treat these items universally as potential biohazards. Do you discard these items in a Biohazard Waste Container,, or do you discard them in a regular trash basket? Thank you in advance for your feedback. Akemi Allison, BS, HT/HTL (ASCP) Histology Manager UMC El Paso, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Plus Slides
Good morning: I just to to thank everyone who responded to my recent inquiry regarding (+) charged slides for IHC. I am getting samples sent to me to test from a few vendors. Thanks again! Akemi Allison, BS, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Plus Slides
Good morning Histo Peeps! I would like to get information on where you are getting your Plus Slides for IHC. We are currently getting our (+) charged slides from Cardinal Health and they have little or no charge. We currently have 2 Ventana IHC Instruments, as well a Special Stainer. We are having tissues falling partially and completely off even on the SS Stainer. Also, I realize Ventana will not back the IHC results unless the end user uses a particular brand of (+) charged slides. Years ago when I worked at Biocare Medical I used to QC our Kling-On Slides before selling them by checking to see if the sections indeed stuck to the slides without any lifting on each lot. I realize that positive charged slides have an expiration life, and these slides are newer lot numbers within their expiration. Thank you in advance for your assistance! Akemi Allison, BS, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] recomendations on new cryostats
Good Morning Histo Peeps! Our facility is looking to purchase 2 new cryostats. Can any of my pathology/histology colleagues recommend which you prefer. Pros and cons of the Sakura and Leica cryostats would be greatly appreciated. Thank you in advance for your input! Warm regards, Akemi Allison, BS HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] test
I received it. I have had the same problem lately. I didn’t get a copy of the email sent. It would be nice to know that it was received. Think they changed their format. Akemi Allison > On Nov 16, 2018, at 7:25 AM, Campbell, Tasha M. via Histonet > wrote: > > I keep trying to send an email for help with an issue I am having and its not > going through. It says the email has been sent but I am not getting the email > and no one has responded. I am testing to see if this message goes through. > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 > > > CONFIDENTIALITY NOTICE: This electronic mail transmission and any > accompanying data files is confidential and is intended exclusively for the > individual or entity to which it is addressed. The communication may contain > information that is proprietary, privileged or confidential or otherwise > legally exempt from disclosure. If you are not the named addressee or you > otherwise have received this message in error, you are not authorized to > read, print, copy or disseminate this message or any part of it. If you have > received this message in error, please notify the sender immediately by email > and delete all copies of this message. Receipt by anyone other than the named > addressee is not a waiver of any attorney-client work product or other > applicable privilege. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stain removal on clothing
Just found this info on-line for stain removal. I assume it originally came from Chuck Churukian since it’s from University of Rochester. REMOVING STAINS FROM HANDS AND CLOTHING Some stains can be removed form the hands by using *Erado-Sol. Dilute Chlorox (or similar bleach) and Erado-Sol are useful for removing stains from white clothing. Care should be exercised in removing stains from colored clothing. To remove the following stains use the solutions indicated. 1.Alcian blue - to remove from the hands use the acid alcohol solution. For cleaning glassware try using dilute Chlorox or acid alcohol. If the stain resists, use them alternatively. 2.Auramine-rhodamine - acid alcohol. 3.Basic fuchsin - Erado-Sol. 4.Carmine - acid alcohol. 5.Chromic acid - concentrated aqueous sodium thiosulfate containing a few drops of sulfuric acid. 6.Crystal violet - Erado-Sol or Chlorox. 7.Eosin Y - saturated aqueous lithium carbonate. 8.Fast green and similar acid dyes - saturated aqueous lithium carbonate. 9.Hematoxylin (alum and iron) - Erado-Sol or acid alcohol. 10. Iodine - 5% sodium thiosulfate. 11. Methylene blue - acid alcohol, Erado-Sol or Chlorox. 12. Nuclear fast red - acid alcohol. 13. Picric acid - saturated aqueous lithium carbonate. 14. Potassium permanganate - 5% oxalic acid. 15. Schiffs solution - Erado-Sol or Chlorox. 16. Silver deposits - Lugols iodine followed by 5% sodium thiosulfate. 17. Sudan stains (Oil red O, Sudan black B) - acetone. 18. Thioflavin T - acid alcohol. *American Scientific Products ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alcian Blue stain on lab coats
Hello Histo Peeps: I have two new lab coats which have been stained with Alcian Blue and can’t get the stains out. I have tried oxy clean incorporated with bleach, as well as pre-soaking in 3% acetic acid, and American Mastertech’s stain remover to no avail. Any suggestions would be greatly appreciated. Thank you in advance, Akemi Allison, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com <mailto:aalli...@montereygi.com> H: Email: akemiat3...@gmail.com <mailto:akemiat3...@gmail.com> Tele: (831) 375-3577 X117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology position in Beautiful Montery, CA
HISTOLOGY POSITION IN BEAUTIFUL MONTEREY, CA ATTENTION HISTOLOGISTS who meet CLIA grossing regulations and are HT or HTL certified. We have an opening in our pathology laboratory to meet the need of our growing medical group. Our lab has a formalin-free environment with new Milestone microwave hybrid tissue processing technology, Leica 2255 microtomes, a new Leica embedding center, a Leica H stainer, and a new Sakura cassette and slide printer. GI experience preferred, but we will train a knowledgeable and willing candidate. We insist on quality embedding and microtomy skills. Duties to include (but not limited to): grossing, embedding, microtomy, limited manual special stains, manual coverslipping, general histology and histology related clerical duties, accessioning, and data entry into our AP Easy LIS System. The right candidate is reliable, self-motivated, articulate, and well versed with anatomy identifications. Must be adept with Microsoft, Excel, and possess excellent keyboarding skills. The lab is located in a private practice Gastroenterology medical group in beautiful Monterey, CA., near the world-famous Pebble Beach and Carmel-by-the-Sea. This job is full-time days, Monday through Friday with NO WEEKEND’s and no travel. We offer a benefits package including medical, dental and vision insurance, paid holidays, vacation, sick accrual and a profit sharing 401K plan. Salary is negotiable depending on experience. Job Type: Full-time Please forward your cover letter and Resume to: Akemi Allison, BS, HT (ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Work: (831) 375-3577 X117 Email: aalli...@montereygi.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Opening in Beautiful Monterey, CA
ATTENTION HISTOLOGISTS! Do you know of anyone who is looking for a job? We have an opening in our pathology laboratory located in a private practice Gastroenterology Medical Group in beautiful Monterey, CA. It is a great opportunity for someone who is HT or HTL certified. The candidate must also meet CLIA grossing regulations. Our lab has a formalin-free environment with new Milestone microwave hybrid tissue processing technology, Leica 2255 Microtome's, New Leica Embedding Center and Leica H Stainer. GI experience helpful, but we will train a knowledgeable and willing candidate. We insist on quality embedding and cutting skills. Duties to include (but not limited to): accessioning, data entry into our AP Easy LIS System, grossing, embedding, microtomy, limited manual special stains, manual coverslipping, general histology and histology related clerical duties. The right candidate is reliable, self-motivated, articulate and well versed with anatomy identifications. Must be adept with Microsoft, Excel, and having excellent typing skills. Full Time Days, Monday through Friday with NO WEEKEND’S! We offer A benefits package including medical, dental and vision insurance, paid holidays, vacation, sick accrual and a profit sharing 401K plan. Salary is negotiable depending on experience. If you are interested, or if you know of someone, contact me and I will give you more details. Akemi Allison, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W.Email: aalli...@montereygi.com H. Email: akemiat3...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Opening in Beautiful Monterey, CA
ATTENTION HISTOLOGISTS! Do you know of anyone who is looking for a job? We have an opening in our pathology laboratory located in a private practice Gastroenterology Medical Group in beautiful Monterey, CA. It is a great opportunity for someone who is HT or HTL certified. The candidate must also meet CLIA grossing regulations. Our lab has a formalin-free environment with new Milestone microwave hybrid tissue processing technology, Leica 2255 Microtome's, New Leica Embedding Center and Leica H Stainer. GI experience helpful, but we will train a knowledgeable and willing candidate. We insist on quality embedding and cutting skills. Duties to include (but not limited to): accessioning, data entry into our AP Easy LIS System, grossing, embedding, microtomy, limited manual special stains, manual coverslipping, general histology and histology related clerical duties. The right candidate is reliable, self-motivated, articulate and well versed with anatomy identifications. Must be adept with Microsoft, Excel, and having excellent typing skills. Full Time Days, Monday through Friday with NO WEEKEND’S! We offer A benefits package including medical, dental and vision insurance, paid holidays, vacation, sick accrual and a profit sharing 401K plan. Salary is negotiable depending on experience. If you are interested, or if you know of someone, contact me and I will give you more details. Akemi Allison, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W.Email: aalli...@montereygi.com <mailto:aalli...@montereygi.com> H. Email: akemiat3...@gmail.com <mailto:akemiat3...@gmail.com> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Supervising a histologist
We are CLIA/CMS certified only. My lab assistant only has a high school degree and never had any background in any sciences. She knows nothing about trouble-shooting, nor theory and practice. She has worked primarily in clerical roles for our GI practice for 13 years. In the past 1.5 years, I trained her to accession, process, embed, cut, stain, and coverslip. We do not do IHC here. Our CEO/GI, MD said she could oversee our new HTL during my absence. Akemi Allison > On Nov 1, 2017, at 5:43 AM, Joseph Saby <saby_josep...@yahoo.com> wrote: > > Eileen- > > I think it would depend on what specific procedures both of them are signed > off on. > And it would be on specific procedures. > Or things are very strange in that area of Histoland. > > Joe Saby > > > From: Eileen Akemi Allison via Histonet <histonet@lists.utsouthwestern.edu> > To: Histonet <Histonet@lists.utsouthwestern.edu> > Sent: Wednesday, November 1, 2017 8:20 AM > Subject: [Histonet] Supervising a histologist > > Hello Histoland: > > This may sound like a strange question, but I have my reasons. Has anyone > run into a situation where a OJT histology assistant supervises an HTL when > the histology manager is absent? Is it even legal? Any feedback would be > greatly appreciated. > > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu <mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Supervising a histologist
Hello Histoland: This may sound like a strange question, but I have my reasons. Has anyone run into a situation where a OJT histology assistant supervises an HTL when the histology manager is absent? Is it even legal? Any feedback would be greatly appreciated. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP question ANP.12500
We keep ours for one week after final report. There have been past issues as to tissues submitted from our endoscopy center not matching up to our gross exams. Gastric tissue included with esophageal tissue, I account for every MINUTE fragment, so there were absolutely floaters from somewhere!. We account for everything when I gross the tissue! I have a embedding log sheet which accounts for every piece submitted, as well as who embeds it, cuts, it, etc.. as well as any comments which just don’t match up to the gross. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com <mailto:aalli...@montereygi.com> H: Email: akemiat3...@gmail.com <mailto:akemiat3...@gmail.com> Tele: (831) 375-3577 X117 Cell: (408) 335-9994 > On Sep 15, 2017, at 8:46 AM, Vickroy, James via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Wondered if something has changed on the question pertaining to retention. > We only do biopsies and keep the containers they come in for two days. We > have done this forever. Going through the new checklist I notice under wet > tissue it has (stock bottle). Before I interpret this incorrectly, a stock > bottle to me meant left over fixed tissue not taken for sections. Last week > I got an email saying this other institution was keeping the empty containers > for two weeks after final report. > > Obviously, it is clear if we have left over tissue we hold it for the > specified time period but does this also mean we should be holding the empty > biopsy container for two weeks plus also? > > I hope I am overthinking this since we don't have room for them. > > Your thoughts? > > Jim > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> > > > > This electronic message contains information from Springfield Clinic, LLP > that may be confidential, privileged, and/or sensitive. This information is > intended for the use of the individual(s) or entity(ies) named above. If you > are not the intended recipient, be aware that disclosure, copying, > distribution, or action taken on the contents of this information is strictly > prohibited. If you have received this electronic message in error, please > notify the sender immediately, by electronic mail, so that arrangements may > be made for the retrieval of this electronic message. Thank you. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017
Tim: I know full well about that facility and have known about their activity for several years! I am at a point in my life I really could care less about any consequences! I am Too old to care about being PC when it comes to the care and nurturing of histologists! I am 67 and all about patient care and what we do to do our best to produce the best quality we can! What can anyone do to me at this point in my life??? Akemi Allison-Tacha BS, HT/HTL ASCP (HT 1968/HTL 1982) > On Aug 1, 2017, at 4:09 PM, Morken, Timothy <Timothy.Morken@uc!sf.edu> wrote: > > OOO, better watch it, that could get some people mad!! > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > -----Original Message- > From: Eileen Akemi Allison via Histonet > [mailto:histonet@lists.utsouthwestern.edu] > Sent: Tuesday, August 01, 2017 3:24 PM > To: Pam Barker; Histonet > Subject: [Histonet] Fwd: RELIA Histology Careers Bulletin - Live where you > vacation 8-1-2017 > > Pam: No one in their right mind would work in that lab in Modesto! They have > a horrible track record for numerous years! Run and don’t even think about > applying! > Akemi > >> Begin forwarded message: >> >> From: "Pam Barker" <rel...@earthlink.net> >> Subject: RELIA Histology Careers Bulletin - Live where you vacation >> 8-1-2017 >> Date: August 1, 2017 at 1:26:51 PM PDT >> To: "Akemi Allison" <akemiat3...@gmail.com> >> >> Hi Akemi! >> What are you doing on your summer vacation? >> Been anyplace you would like to move to? >> Whatever you want to do, wherever you want to be, I can help! >> >> There is still time this summer to make that move. >> I have opportunities in all kinds of labs in all areas of the country. >> Your next position is just a phone call or an e-mail away. >> >> All of the opportunities that I represent are permanent full time positions >> with companies that offer excellent salaries, benefits and relocation >> assistance. >> >> Here are some of the opportunities that I am most excited about: >> >> HISTOLOGY SUPERVISORS AND MANAGERS: >> Histology Lab Manager – Atlanta, GA >> Histology Supervisor – Atlanta, GA >> Histology Supervisor – Boston, MA >> Histology Supervisor – Irvine, CA >> Histology Supervisor – Modesto, CA >> Lead Histotech – Los Angeles – BRAND NEW LAB Study Director – Fort >> Collins CO HISTOTECHNICIANS/HISTOTECHNOLOGISTS >> CA – San Diego – brand new lab CLIA qualified to gross CA – Irvine – >> learn IHC CA – Los Angeles – Lead Histotech Brand New Lab! >> TX – Nacogdoches day shift hospital environment CO – Glenwood day >> shift hospital environment TN – Chattanooga – CLIA qualified to gross >> 3rd shift generous shift diff. >> MA – Greater Boston Area - 2nd and 3rd shift generous shift diff. >> >> If you happen to know anyone I am also looking for a cytotech in >> Clearwater, FL >> >> If you or anyone you know are interested in hearing more about any of these >> opportunities or have another type of position or area in mind and would >> like some help in your job search please let me know. Just shoot me an >> e-mail at rel...@earthlink.net <mailto:rel...@earthlink.net> or give me a >> call toll free at 866-607-3542 or on my cell at 407-353-5070. >> >> Thanks-Pam >> >> Right Place, Right Time, Right Move with RELIA! >> >> Thank You! >> Pam M. Barker >> >> Pam Barker >> President/Senior Recruiting Specialist-Histology RELIA Solutions >> Specialists in Allied Healthcare Recruiting >> 5703 Red Bug Lake Road #330 >> Winter Springs, FL 32708-4969 >> Phone: (407)657-2027 >> Cell: (407)353-5070 >> FAX: (407)678-2788 >> E-mail: rel...@earthlink.net <mailto:rel...@earthlink.net> >> https://www.facebook.com/RELIASolutionsforhistologyprofessionals >> <https://www.facebook.com/RELIASolutionsforhistologyprofessionals> >> www.facebook.com >> <http://www.facebook.com/PamBarkerRELIA>/PamBarkerRELIA >> www.linkedin.com/in/reliasolutions >> <http://www.linkedin.com/in/reliasolutions> >> www.twitter.com/pamatrelia <http://www.twitter.com/pamatrelia> > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fwd: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017
Pam: No one in their right mind would work in that lab in Modesto! They have a horrible track record for numerous years! Run and don’t even think about applying! Akemi > Begin forwarded message: > > From: "Pam Barker" <rel...@earthlink.net> > Subject: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 > Date: August 1, 2017 at 1:26:51 PM PDT > To: "Akemi Allison" <akemiat3...@gmail.com> > > Hi Akemi! > What are you doing on your summer vacation? > Been anyplace you would like to move to? > Whatever you want to do, wherever you want to be, I can help! > > There is still time this summer to make that move. > I have opportunities in all kinds of labs in all areas of the country. > Your next position is just a phone call or an e-mail away. > > All of the opportunities that I represent are permanent full time positions > with companies that offer excellent salaries, benefits and relocation > assistance. > > Here are some of the opportunities that I am most excited about: > > HISTOLOGY SUPERVISORS AND MANAGERS: > Histology Lab Manager – Atlanta, GA > Histology Supervisor – Atlanta, GA > Histology Supervisor – Boston, MA > Histology Supervisor – Irvine, CA > Histology Supervisor – Modesto, CA > Lead Histotech – Los Angeles – BRAND NEW LAB > Study Director – Fort Collins CO > HISTOTECHNICIANS/HISTOTECHNOLOGISTS > CA – San Diego – brand new lab CLIA qualified to gross > CA – Irvine – learn IHC > CA – Los Angeles – Lead Histotech Brand New Lab! > TX – Nacogdoches day shift hospital environment > CO – Glenwood day shift hospital environment > TN – Chattanooga – CLIA qualified to gross 3rd shift generous shift diff. > MA – Greater Boston Area - 2nd and 3rd shift generous shift diff. > > If you happen to know anyone I am also looking for a cytotech in Clearwater, > FL > > If you or anyone you know are interested in hearing more about any of these > opportunities or have another type of position or area in mind and would like > some help in your job search please let me know. Just shoot me an e-mail at > rel...@earthlink.net <mailto:rel...@earthlink.net> or give me a call toll > free at 866-607-3542 or on my cell at 407-353-5070. > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: rel...@earthlink.net <mailto:rel...@earthlink.net> > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > <https://www.facebook.com/RELIASolutionsforhistologyprofessionals> > www.facebook.com <http://www.facebook.com/PamBarkerRELIA>/PamBarkerRELIA > www.linkedin.com/in/reliasolutions <http://www.linkedin.com/in/reliasolutions> > www.twitter.com/pamatrelia <http://www.twitter.com/pamatrelia> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] I have a job
I thought we weren’t allowed to post jobs on histonet! I tried and it was removed! Akemi Allison-Tacha > On Mar 27, 2017, at 11:45 AM, Caryn Anderton via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Hello- > > I wanted to know if it was possible to post a position that I have available? > See Below > > Immunohistochemistry Product Manager Communications Expert- Part Time > > Summary: > The position will focus on working with three internal groups within Biocare > Medical: Marketing, Engineering and R We're looking for a driven, > resourceful technical writing and communication expert to manage the process > of writing our customer-facing product documentation. You'll manage the > end-to-end process of drafting, writing, and releasing documentation, > tutorials, developer guides for: internal and client trainings, sales > collateral and product manuals. > Engineering & R: > Collaborate with the Engineering team to launch a new automated IHC slide > staining platform. Engage with the teams to understand the instrument and how > it works and be able to translate technical concepts, and make them easy to > understand for our end users. Create and maintain reference and user guides, > including hardware and software documentation > Marketing: > Work with marketing to supply scientific knowledge and technical/medical > writing for product catalogs, marketing brochures and website content. Work > with training department to develop sales training programs. > Requirements: > > ? Broad knowledge base in Histology , Immunohistochemistry and related > instrumentation > > ? Excellent writing, editing, and proofreading skills with meticulous > attention to detail > > ? Ability to present information and generate content appropriate market > segments > > ? Self-motivated, independent, able to prioritize and manage multiple > projects > > ? Demonstrate capacity to work collaboratively within cross-functional teams > > ? Proven and consistent track record of meeting project deadlines with > quality results > > ? Excellent organizational and time management skills > > ? Ability to receive creative direction and feedback > > ? Please include a cover letter and curriculum-writing sample with your > application. > Biocare Medical is a leading supplier of automated immunohistochemistry (IHC) > instrumentation, antibodies and the full range of reagents for IHC. Biocare's > portfolio includes novel and established primary antibodies and FISH probes > for many tissue types. > EEO/AA Employer/Veterans/Disabled/Race/Ethnicity/Gender/Age > > To all recruitment agencies: Biocare does not accept agency resumes. Biocare > will not pay for any fees related to unsolicited resumes. > > Job Location-Pacheco, California, United States > Department-Marketing > Position Type-Part Time > > > > > > > Caryn M. Anderton > Senior Director of Marketing > Biocare Medical, LLC. > 60 Berry Dr. > Pacheco, CA 94553 > (925) 768-1270 > www.biocare.net<http://www.biocare.net/> > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] I have a job
I thought we weren’t allowed to post jobs on histonet! I tried and it was removed! Akemi Allison-Tacha > On Mar 27, 2017, at 11:45 AM, Caryn Anderton via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Hello- > > I wanted to know if it was possible to post a position that I have available? > See Below > > Immunohistochemistry Product Manager Communications Expert- Part Time > > Summary: > The position will focus on working with three internal groups within Biocare > Medical: Marketing, Engineering and R We're looking for a driven, > resourceful technical writing and communication expert to manage the process > of writing our customer-facing product documentation. You'll manage the > end-to-end process of drafting, writing, and releasing documentation, > tutorials, developer guides for: internal and client trainings, sales > collateral and product manuals. > Engineering & R: > Collaborate with the Engineering team to launch a new automated IHC slide > staining platform. Engage with the teams to understand the instrument and how > it works and be able to translate technical concepts, and make them easy to > understand for our end users. Create and maintain reference and user guides, > including hardware and software documentation > Marketing: > Work with marketing to supply scientific knowledge and technical/medical > writing for product catalogs, marketing brochures and website content. Work > with training department to develop sales training programs. > Requirements: > > ? Broad knowledge base in Histology , Immunohistochemistry and related > instrumentation > > ? Excellent writing, editing, and proofreading skills with meticulous > attention to detail > > ? Ability to present information and generate content appropriate market > segments > > ? Self-motivated, independent, able to prioritize and manage multiple > projects > > ? Demonstrate capacity to work collaboratively within cross-functional teams > > ? Proven and consistent track record of meeting project deadlines with > quality results > > ? Excellent organizational and time management skills > > ? Ability to receive creative direction and feedback > > ? Please include a cover letter and curriculum-writing sample with your > application. > Biocare Medical is a leading supplier of automated immunohistochemistry (IHC) > instrumentation, antibodies and the full range of reagents for IHC. Biocare's > portfolio includes novel and established primary antibodies and FISH probes > for many tissue types. > EEO/AA Employer/Veterans/Disabled/Race/Ethnicity/Gender/Age > > To all recruitment agencies: Biocare does not accept agency resumes. Biocare > will not pay for any fees related to unsolicited resumes. > > Job Location-Pacheco, California, United States > Department-Marketing > Position Type-Part Time > > > > > > > Caryn M. Anderton > Senior Director of Marketing > Biocare Medical, LLC. > 60 Berry Dr. > Pacheco, CA 94553 > (925) 768-1270 > www.biocare.net<http://www.biocare.net/> > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cassette Printers
Good morning Histoland! Our department is looking into purchasing a new cassette printer which has the capability of interfacing with our AP Easy LIS System. I am currently demoing a General Data ID/Positive CL-01 Laser Cassette Printer and label printer system. We are a fairly small lab with limited space so a small foot print is essential. We also do not need a multiple magazine. I would love to hear your comments, good, bad or indifferent… Thanks in advance Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com <mailto:aalli...@montereygi.com> H: Email: akemiat3...@gmail.com <mailto:akemiat3...@gmail.com> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cassette printer additional request
Hi Again: I forgot to mention I am open to investigate other Cassette Printers other than the General Data Printer… AlOf course, price, and dependability is a big factor. Would love to hear your comments, good, bad or indifferent... Thanks in again! Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com <mailto:aalli...@montereygi.com> H: Email: akemiat3...@gmail.com <mailto:akemiat3...@gmail.com> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RIP Wanda Platt Jones
My feelings for Wanda Platt Jones: Although I am Buddhist, and believe in reincarnation, I am untuned to all religions! In Wanda's case, perhaps, she was needed to be with her GOD to help serve in the best way possible for the ultimate good of everyone. She had an energy and presence which was focused unconditionally for true GOOD and LOVE for everyone. Wanda to me is a True Angel; who now is looking down on us, and helping us to become better human beings. Anyone she came in contact with benefitted from her remarkable love, sweetness, and acceptance. I will truly miss her, but I know she has not left us. She is hugging and guiding us, and showering us with her love and sweetness. She is sitting in a Prestigious Histology Chair in the Sky with all the Greats, such as the likes of Chuck Churukian. He was another Great Christian Histologist, and a Very, Dear Friend! XoXo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Hire for My Lab
Attention West Coast Histology Students and Graduates! If you will be graduating, and are eligible for certification, or are recently graduated and certified; our GI Endoscopy laboratory in Beautiful Monterey, CA is expanding, and we are going to hire another histologist. We have a formalin free environment, with new Microwave Hybrid Tissue processing Technology. This is a great opportunity for the right person. WILL TRAIN a knowledgeable, willing candidate. Duties to include (but not limited to): accessioning, data entry into AP Easy LIS System, grossing, embedding, microtomy, and general histology *must be self motivated, reliable and a team player. Preferred that you meet CLIA grossing regulations. Monday through Friday with no weekends. We offer full benefits and 401 K. Salary is negotiable. If you are interested, or if you know of someone, contact me and I will give you more details. akemiat3377@gmail.comStudents! If you will be graduating and are eligible for certification, or are certified histologists; our GI Laboratory in Beautiful Monterey, CA is expanding, and we are going to hire another histologist. Preferred that you meet CLIA grossing regulations. This is a great opportunity for the right person. WILL TRAIN a knowledgeable, willing candidate. Duties to include (but not limited to): accessioning, data entry into AP Easy LIS System, grossing, embedding, microtomy, and general histology *must be self motivated, reliable and a team player. Monday through Friday with no weekends. We offer full benefits and 401 K. Salary is negotiable. If you are interested, or if you know of someone, contact me and I will give you more details. akemiat3...@gmail.com Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com <mailto:aalli...@montereygi.com> H: Email: akemiat3...@gmail.com <mailto:akemiat3...@gmail.com> Tele: (831) 375-3577 X117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] block retention
10 years for all slides and blocks with the exception of pediatric cases. Those cases we keep forever. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com <mailto:aalli...@montereygi.com> H: Email: akemiat3...@gmail.com <mailto:akemiat3...@gmail.com> Tele: (831) 375-3577 X117 Cell: (408) 335-9994 > On Nov 8, 2016, at 8:12 AM, Teresa W. Gainey via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > We keep our blocks for 15 years. Slides foreverper Pathologist request. > > > Teresa W.Gainey > > Beaufort Memorial Hospital > > Pathology Team Coordinator > > 843-522-5159 > > > > From: Algeo, Lacie A <lacie.al...@providence.org> > Sent: Thursday, November 3, 2016 11:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] block retention > > Hi All, > Outside of the current guidelines, how long is everyone saving their > blocks/slides. > Thanks, > Lacie > > Lacie Algeo, HTL (ASCP) MBCM > Histology Supervisor > Providence Sacred Heart Medical Center Laboratory > 101 W 8th Avenue > L-2 > Spokane, WA 99204 > 509-474-4418 > FAX 509-474-2052 > lacie.al...@providence.org<mailto:lacie.al...@providence.org> > > > This message is intended for the sole use of the addressee, and may contain > information that is priviledged, confidential and exempt from disclosure > under applicable law. If you are not the addressee, you are hereby notified > that you may not use, copy, disclose or distribute to anyone the message or > any information contained in the message. If you have received this message > in error, please immediately advise the sender by reply e-mail and delete > this message. > > > > > This message is intended for the sole use of the addressee, and may contain > information that is privileged, confidential and exempt from disclosure under > applicable law. If you are not the addressee you are hereby notified that you > may not use, copy, disclose, or distribute to anyone the message or any > information contained in the message. If you have received this message in > error, please immediately advise the sender by reply email and delete this > message. > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hiring a histologist
Attention recently graduated certified or certified eligible histologists! Our GI practice in Beautiful Monterey, CA is expanding, and we are going to hire another histologist to hone their skills under my tutelage. Must meet CLIA grossing regulations. We offer full benefits and 401 K. If you are interested, or know of someone who would be interested, contact me and I will give you more details. akemiat3...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Temperature for slides
We monitor room temp and record it as per CAP, and CLIA. Are you sure the request wasn’t for monitoring room temp because of paraffin blocks, not slides? The concern is for paraffin blocks melting, due to excessive heat. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.com mailto:aalli...@montereygi.com Tele: (831) 375-3577 X117 On Aug 28, 2015, at 4:58 AM, Heckford, Karen - SMMC-SF via Histonet histonet@lists.utsouthwestern.edu wrote: Okay I just got a weird request for monitoring the room temperature where I have my slides for that I use for Histology. We are talking blank slides. I have never heard of this before. Who else does this??? Apparently JCAHO wants this. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Marciafava-Bignami Disease I have re-logged into the system and maybe this will now communicate with Histologists out there, and hope that I do not get twice as many emails from Histone
GP’s are fine for general health issues, but I would certainly get more conclusive tests done by a neurologist, as well as contacting the best worker’s comp attorney in your area who has dealt with chemical exposure cases. Studies show we are #1 in this country for the most hazardous professions. It's safe compared to when I 1st started in this field in 1965! Well, I am a dinosaur from back in the day when we had inadequate ventilation, a shortage of fume hoods, inadequate education on hazards, safety and PPE's. In 1979 I started to work at OHSU in the surgical path lab, as well as doing research projects. We made up all of our own HE's and special stains from scratch, as well as made up our own 10% NBF in 55 gallon drums without fume hoods, ventilation, masks or gloves. I also worked with Glyco-Methacrylate embedded tissues without hoods or gloves! Since it was a medical school, we did every special stain under the sun and dealt with about every chemical, reagent, acid and stain you could think of! We also smoked cigarettes and drank coffee in the lab while we embedded and cut! We sure were a naive group back then! In the early days, the facilities I worked in never had MSDS information available. In 1989, while at Emanuel Hospital, Portland, OR, I researched and compiled the MSDS information on all the chemicals, reagents, acids and stains that we used. That was the 1st eye opener to what me and my fellow histologists dealt with on a daily basis. In 1988 I had base line tests done because I was having issues with dizziness, balance, reflexes, and short term memory loss. In 1992, I had extensive neurological tests done, as well as a sural nerve bx taken from my right ankle. It was found that I had nerve damage, loss of balance, no reflexes, numbness in my fingers, hyper sensitivity and reduced feeling on my right side, hearing loss in my left ear, and an aedes pupil in my right eye. It was concluded these were the results from exposure to multiple toxic chemicals in an extremely small room with excessive heat and NO VENTILATION at current hospital I was working at. These health issues are irreversible. I just deal with it. I was the 1st person who won a case for this in the state of Oregon, but it had consequences. I won the battle, but lost the war! I now have Primary Biliary Cirrhosis (PBC). I was 1st diagnosed in 2008 for PBC. I think this condition was caused by my continual exposure to Multiple Toxic Chemicals. You may, or may not agree. A huge amount of the chemicals we deal with in the histology lab targets the liver and is absorbed through the skin or is inhaled. Here is the link for PBC. http://www.liverfoundation.org/abouttheliver/info/pbc/ http://www.liverfoundation.org/abouttheliver/info/pbc/ Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com mailto:aalli...@montereygi.com On Aug 4, 2015, at 4:47 AM, Edmondson David (RBV) NHS Christie Tr via Histonet histonet@lists.utsouthwestern.edu wrote: __ From: Edmondson David (RBV) NHS Christie Tr Sent: 02 August 2015 16:40 To: 'histonet@lists.utsouthwestern.edu' Subject:Marciafava-Bignami Disease Hello out there, I have been working in Histology for forty years and am wondering at a cause for this disease. It was seen on MRI scans and they have told me to ease up on the alcohol, but I have never been remotely alcholic so I am giving up work in October and hope to live a little longer. Addition to work has been my only problem. They have tested a variety of bodily functions, touching my nose and foot movements together with assessing my ability to ride my motorcycle and make toast and tea, and then they let me off the acute neuro ward. My bike was chained up outside and making my way between lines of static traffic should have been a good sign. I am currently on massive doses of B vitamins but my GP reckons that they are of no use and I should just get better. So, is Xylene at the root of all my demyelination?? Regards David Edmondson This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Marciafava-Bignami Disease
From my archives regarding my dear friend (Chuck) Charles Churukian: December 26, 2010 https://www.facebook.com/akemi.allison/posts/176373569052385 · https://www.facebook.com/# I just heard from Irene Churukian today. Please put Chuck in your prayers. Irene said this: CLL cells changed in character so Chuck began chemo treatments on Dec. 1. His second one was Dec. 21... He has six altogether -The first cycle was difficult... he was very weak-in bed most of the time. He is now improving-thankfully. We are praying that by April, he will be in remission after all the treatments are completed. RIP Chuck Xo Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com mailto:aalli...@montereygi.com On Aug 4, 2015, at 2:11 PM, Ingles Claire via Histonet histonet@lists.utsouthwestern.edu wrote: I know I watch my exposure to Formalin and Xylene especially. I was diagnosed with NASH in 2007 (who knows how long before then, since I am asymptomatic) when I had a Pituitary Adenoma that had hemorraged (Boy talk about a headache!) Anyway, I have always wondered about the cause. I thought it was genetic as my father also had it for over 40 years before finally having a transplant. But I wonder if it was his exposure to jet fuel, etc. as he was a jet mechanic for 20 years in the USAF plus another 15-20 with a private company. So far my liver has been stable even getting a bit better to the point of being just a hair above the normal range. I do everything under the sun in my lab. Mohs, Grossing, plus all the routine stuff of cutting and staining, etc. I have been able so far to keep the repetitive injuries at bay, and Dansco shoes have saved my feet from the Plantar Faciitis (had that about a year straight at one point.). We had managed to keep xylene out of our lab for years by using Propar, but one of the Pathologists insisted on having a tape coverslipper. We still use it as little as possible. I had a co-worker, when she was pregnant wasn't allowed to gross (hence contact with formalin). Because of the NASH I get blood tests done every 6 months. It will be interesting to see if they have changed possibly due to the exposure to xylene, which I haven't had exposure to for years otherwise. Claire From: Mayer,Toysha N via Histonet [histonet@lists.utsouthwestern.edu] Sent: Tuesday, August 04, 2015 2:49 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Marciafava-Bignami Disease This is very interesting. I have worked in the field for over 20 yrs, and was pregnant while working in a small lab. Mostly everything was manual, and I did not use all of the safety precautions I should have (my fault). My son had severe speech and language delay as well as a language processing issue when he was smaller, and now stutters. I have often wondered (as most parents would) if my lack of precautions may have contributed. We stress the safety issues to the students daily, to get it all in their heads. I often tell people that I am so happy because I work with xylene, and I feel real good. So far, I don't see any noticeable symptoms of anything wrong, just the usual-decreased olfactory sensing, repetitive motion issues, plantar fasciitis, and varicose veins. Hair loss was an issue at one time, but that was attributed to PCOS. Now that I am not in the lab full time anymore it has decreased since I went natural (I'm African-american). One thing I have noticed is my C-reactive protein is high, and I take a statin for that. I have no liver issues, and my functions appear to be normal. Next time I go in for a physical, I will have my workplace hazards documented in my EMR. I will look up Primary Biliary Cirrhosis (PBC), to get some more information, especially since I was born jaundiced and I am anemic. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 11 Date: Tue, 4 Aug 2015 06:30:12 -0700 From: Eileen Akemi Allison akemiat3...@gmail.com To: Edmondson David (RBV) NHS Christie Tr david.edmond...@christie.nhs.uk Cc: Histonet histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Marciafava-Bignami Disease I have re-logged into thesystem and maybe this will now communicate with Histologists out there, and hope that I do not get twice as many emails from Histonet# Message-ID: e3d7c5a9-c516-4f0e-b522-61b348820...@gmail.com Content-Type: text/plain; charset=utf-8 GP?s are fine for general health issues, but I would certainly get more conclusive tests done by a neurologist, as well as contacting the best worker?s comp attorney in your
Re: [Histonet] GI Pathology Req's
Just an FYI- We do not have a LIS System! The GI CEO and administrator DOES NOT want to invest in a LIS System! Our pathologists are contracted, so they just go with the flow… which is difficult for me to have support to upgrade our system. As usual, we are an after thought! When I came here 4 years ago, I thought I was in a time machine and had been transported back to 1989 before they had lab computers or LIS Systems! We are currently using “PCM”. It is geared towards GI MD’s and their practice. It is a system which is used for scheduling and all patient information. It interfaces with the billing system. This system was in place before I got here. Currently, my assistant has to create a Path Report and type in gross, etc… then the pathologist types in his microscopic findings and sign’s the report. Everything is hard copy….. I have basically time warped to before the 90’s when I created all these workload recording forms up. We have hard copy req forms which are hand written by the nurses in endoscopy with all the patient and specimen information. We do have an automated cassette printer, but we hand write slides. We hand write an embedding log with # of number of pieces in the cassette, etc…for QC purposes. I hand write CPT charges on a hard copy and submit to the billing dept. Nothing is bar coded, etc…. It is better now that I have an assistant. The endoscopy center has upgraded to “Provation”. It interfaces with our current “PCM” (Prime Clinical Management System) which is antiquated! The new computer system for scheduling and billing is Athena Healthcare” it is geared for billing and appointment scheduling etc We are also interfaced with Elkay? and are in training now and go live August 11th! We are still going to have to do the same procedure with pathology as usual, but we have to create the templates again! They are not getting us a LIS system to interface! Oh wait, I’ve been a histologist for 50 years and still going strong! It’s frustrating, but at least I live in a beautiful area! Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com mailto:aalli...@montereygi.com H: Email: akemiat3...@gmail.com mailto:akemiat3...@gmail.com Tele: (831) 375-3577 X117 Cell: (408) 335-9994 On Jun 29, 2015, at 7:05 PM, Cristi Rigazio cls71...@gmail.com wrote: I am not at work right now but we have some great ideas. What EMR and LIS are you using? Sent from my iPhone On Jun 29, 2015, at 6:04 PM, Eileen Akemi Allison akemiat3...@gmail.com wrote: Hello Histolandl! I would like to reach out to all of you who do GI pathology. Our facility is going through a computer systems change for our endoscopy center, as well as the upstairs doctors offices, billing department, and the AP lab. I was wondering if any of you would care to share your GI requisition form. We would like to incorporate a new version for our endoscopy center. I would greatly appreciate it. Please send to my email address. Thank you in advance for your assistance! Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com mailto:aalli...@montereygi.com H: Email: akemiat3...@gmail.com mailto:akemiat3...@gmail.com Tele: (831) 375-3577 X117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GI Pathology Req's
Hello Histolandl! I would like to reach out to all of you who do GI pathology. Our facility is going through a computer systems change for our endoscopy center, as well as the upstairs doctors offices, billing department, and the AP lab. I was wondering if any of you would care to share your GI requisition form. We would like to incorporate a new version for our endoscopy center. I would greatly appreciate it. Please send to my email address. Thank you in advance for your assistance! Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com mailto:aalli...@montereygi.com H: Email: akemiat3...@gmail.com mailto:akemiat3...@gmail.com Tele: (831) 375-3577 X117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] searching for a 50cm stainless steel profile A knife
Maria: Hacker Instruments sells new and reconditioned knives. They also provide a knife sharpening service for a fee of course. I believe they have that size, but if not, they will help you. Here is their link. http://www.tidylabstore.com/newmikn.html Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com mailto:aalli...@montereygi.com H: Email: akemiat3...@gmail.com mailto:akemiat3...@gmail.com Tele: (831) 375-3577 X117 On Jun 9, 2015, at 8:28 PM, Maria Mejia mbmph...@gmail.com wrote: Hello, Can any one tell me where I can buy a 50cm stainless steel profile A type knife to use on a tetrander cast iron sliding microtome? Any assistance from anyone will be greatly appreciated. Regards Maria Mejia UCSF Mission Bay San Francisco, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Athena Healthcare computer system
Good Morning and Happy Friday Histoland! Just curious if anyone out there is using the Athena Healthcare Computer system for your AP lab. If so, do have any special Macros you have created, or do you use a separate LIS system? I would greatly appreciate any feedback you can provide. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com mailto:aalli...@montereygi.com H: Email: akemiat3...@gmail.com mailto:akemiat3...@gmail.com Tele: (831) 375-3577 X117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Athena Healthcare computer system
Good Morning and Happy Friday Histoland! Just curious if anyone out there is using the Athena Healthcare Computer system for your AP lab. If so, do you have any special Macros you have created, or do you use a separate LIS system? From my research, it appears to be a billing a patient scheduling system. I would greatly appreciate any feedback you can provide. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aalli...@montereygi.com mailto:aalli...@montereygi.com H: Email: akemiat3...@gmail.com mailto:akemiat3...@gmail.com Tele: (831) 375-3577 X117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P26 billing for IHC Professional component
Good morning Histoland: Happy Friday! I was asked by a friend to post this on histonet. They have a question for those of you who may be familiar with billing for the IHC professional component only. Here is the scenario; a small lab who does not perform IHC in-house sends a block to be cut and stained for IHC to a reference lab who performs a stain only IHC. The reference lab then sends the stained slides back to the ordering facility and their pathologist makes the interpretation of the stained slides. Arrangements have been made for the reference lab to charge the patients insurance company and/or patient for the technical component of the IHC stain. How would the requesting facility billing department charge for the pathologists professional component? Do they charge with a P26 CPT code only? Thank you in advance for your responses! Akemi Allison BS, HT/HTL (ASCP) President Phoenix Lab Consulting Marina, CA 93933 Tele: (408) 335-9994 Email: akemiat3...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Levels for Gastic and Esophogeal Bx's
Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx’s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Water baths
Speaking for myself and the labs I have managed, I have been checking ALL temps and recording them since the early 90’s. These include waters baths for tissue flotation, and ones used for special stains, ovens, refrigerators, freezers, cryostats, tissue processors, embedding centers, decloaking chambers and room temps. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.com Tele: (831) 375-3577 X117 On Dec 2, 2014, at 7:29 AM, Abbott, Tanya tanyaabb...@catholichealth.net wrote: Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On the lighter side...
49 years and 45 years certified as HT and 22 as HTL Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.com Tele: (831) 375-3577 X117 On Aug 7, 2014, at 3:39 PM, Ingles Claire cing...@uwhealth.org wrote: 15 years and over 10 certified HTL. Claire Ingles From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Richard A. Carter [rcar...@cancer-test.com] Sent: Thursday, August 07, 2014 4:36 PM To: 'Jennifer MacDonald'; Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 28 years Rick Carter -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, August 7, 2014 2:20 PM To: Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 31 years Jennifer MacDonald From: Douglas Porter doug.por...@caplab.org To: histonet@lists.utsouthwestern.edu Date: 08/07/2014 11:36 AM Subject:[Histonet] On the lighter side... Sent by:histonet-boun...@lists.utsouthwestern.edu How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Friday histology trivia
Glad to know I was correct all these years! Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.com Tele: (831) 375-3577 X117 On Jun 27, 2014, at 12:28 PM, Jan Shivers shive...@umn.edu wrote: The Ki in Ki-67 is from the city of origin (Kiel, Germany), and is really pronounced KEY-67 (not K. I. 67). The 67 in Ki-67 is from the original clone in a 96-well plate. Happy Friday! -- Jan Shivers Senior Scientist University of Minnesota On Fri, Jun 27, 2014 at 1:24 PM, Morken, Timothy timothy.mor...@ucsfmedctr.org wrote: The LEI in Leic comes from Leitz; the CA, from Cambridge. Barbara, You get the award for best trivia fact of the year! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Foster Sent: Friday, June 27, 2014 10:27 AM To: Histonet Subject: [Histonet] Friday histology trivia You are so right. Microscopes equal credibility. Maybe because we can actually see an image of the specimen rather than a squiggly line... or maybe because people actually sit at microscopes rather than at an impersonal control panel. Re: AO - They were part of the American Optical/Reichert-Jung Family. In 1986, they were acquired by Cambridge Instruments, which then merged with Leitz in 1991. The LEI in Leic comes from Leitz; the CA, from Cambridge. I had the privilege of being the Technical Marketing Manager for the research microscopy group, then, for a short period, product development manager for a suite that included the AO clinical line. Best regards, Barbara Foster ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Project DNA-Hereditary Cancers
FYI to my Histonet peeps. New developments in Hereditary Cancer. I am so proud to be associated with such a progressive GI doctor who has a passion towards genetic cancer solutions! Check out what the CEO of our GI practice/pathology laboratory, Dr. Daniel Luba's passion is on this website. He is the Chairman of the Board for project DNA. http://theprojectdna.com/about/ With warmest regards, Akemi Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.com Tele: (831) 375-3577 X117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Was told I am having bounce-backs
This is a test to see if this posts on histonet. I was told yesterday that there are a number of bounce-backs. I haven't received emails from histonet the past few days. Anyone else having problems? Peculier issues with yahoo lately Akemi Allison-Tacha, BS, HT (ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577 X117 W. Email: aalli...@montereygi.com P. Email: akem...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Our Day!
Happy Histotechnology Professionals Day to All my Colleagues! Did you know it is also Dezna Sheehan's Birthday too? I am honored to have known her. What an incredible mentor. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.com Tele: (831) 375-3577 X117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mineral Oil in Microwave Processing.
I have a Milestone Microwave Tissue Processor and use Prowave from Milestone. It’s proprietary, but it has alcohol in it and does a great job on our tissue.I haven’t used mineral oil for years! Akemi Allison HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aalli...@montereygi.com (831) 375-3577 X117 On Feb 16, 2014, at 6:44 PM, Cesar Francisco Romero chesar...@hotmail.com wrote: Dear People from Histonet Do you still use Mineral Oil in your Microwave Processing Protocol ? Or you only use ethyl alcohol ~ Isopropyl alcohol and Paraffin . I will apreciate any advice. Thank you all Cesar Romero Buenos Aires Argentina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Trade H-Pylori for Fungus Control
Hi everyone in histo land! I hope you all had a wonderful Valentines Day. I am hoping I might get a Valentines gift of some fungus control tissue. I am getting down to the tail end of mine. I would be more than happy to trade some (+) H Pylori blocks in exchange. I would be more than happy to pay for the shipping charges. Thank you in advance, Akemi Allison, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory Email: aalli...@montereygi.com Tele: (831) 375-3577 X117 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Jules Elias IHC Award
Congratulations Jim Burchette on receiving the Jules Elias IHC Award. Well deserved! Just emailed Jules to let him know. Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CLIA Compliance Regulations for histology staff coverage
Good morning to all in Histoland! Hopefully, one of you can help me with a question which was proposed to me by a fellow histologist working in a very small GI histology lab. Here is what information she gave me: She is training a histology assistant in house to do all the histology technical responsibilities at the request of the GI doctors. She said her OJT assistant is doing a great job technically, but she has not yet registered in school to finish her AA Degree so she can sit for the HT exam. She has been working under her instruction for 1 year. Her lab is California State and CLIA licensed. The GI doctors want the assistant to cover for her while she is on vacation. The pathologist who is the medical director does not think that CLIA Regulations allows this and wants the specimens sent out during her absence. She needs the CLIA Regulations stating what the guidelines are so her lab is complying to regulations. She didn’t want to call CLIA because it would send a RED FLAG up. I also didn’t want to call them because they may ask me what the name of the lab was that was proposing this question. I told her that this was most likely illegal, but she needs it in black and white. Do any of you have that information or a web link to go to at your finger tips? She needs the regulations by next week. Thank you in advance for your help, Akemi Akemi Allison-Tacha, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577 X117 Email: aalli...@montereygi.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Training and Competency checklist
Hi Again in Histoland. I have another favor to ask. I was going to give my friend a Histology Training and Compentency Checklist, but I had it on my old computor and didn't transfer it to my new MAC. I only have my Compentency checklist for Client Services from PhenoPath. Would anyone like to share theirs with me? Thank you in advance for your assistance, Akemi Akemi Allison-Tacha, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577 X117 Email: aalli...@montereygi.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] periodic acid/he
I forgot to mention the timing. You may try 5 minutes or even 10 minutes before staining in hematoxylin. It will give you even better results. I used 5 minutes in 1 % periodic acid. Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com From: e...@pigsqq.org e...@pigsqq.org To: histonet@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Sent: Friday, July 5, 2013 6:27 AM Subject: [Histonet] periodic acid/he Thanks for the tip. I had some periodic acid so I tried a 2 minute soak in a 0.5% soln of that followed by a tap water wash and then dw then Harris hematoxylin countered with a Eosin-Biebrich scarlet we like on some classical swine fever slides I looked at today. Very nice. I will play with this more. By the way, We use the hot water/detergent method for all our dewaxing now. We still are using xylene and alcohols for our processing from fixative to paraffin but we will change that eventually. E. Wayne Johnson Enable Ag Tech Beijing ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: HE staining question
Tony: I actually was going to suggest this. You beat me to it! I used to do GMA on 1.5 microns sections and did a Harris hematoxylin and eosin/phloxine routinely (by the way, this was not recommended back in 1980. Gills hematoxylin was the norm!). To get the results I wanted, I would routinely oxidize the slides in 1% periodic acid and rince in running tap water before staining in hematoxylin. the staining was intensified and was beautiful! I came up with this because my PAS's with hematoxylin counterstains looked better than routine HE's. I figuired it was worth doing the GMA's routinely using 1% periodic acid since we didn't do that many. Funny how we stumble on our modifications! Science never sleeps!! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com From: Tony Reilly tony_rei...@health.qld.gov.au To: Teri Johnson tjohn...@gnf.org; Linda Prasad (SCHN) linda.pra...@health.nsw.gov.au Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, July 3, 2013 4:06 PM Subject: [Histonet] Re: HE staining question Hi Linda For a little more information (not an article) go to: http://www.ihcworld.com/royellis/problems/problem21.htm regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ Teri Johnson tjohn...@gnf.org 7/4/2013 12:37 am Hi Linda, There is no journal article, merely an observation on my part many years ago. Teri On Jul 2, 2013, at 10:05 PM, Linda Prasad (SCHN) linda.pra...@health.nsw.gov.au wrote: Hi Teri, Oxidising the slides in periodic acid prior to HE improves the HE. Would really appreciate if you could please send me the reference for that article. Thank you Teri. Linda Prasad, MSc, BSc Hospital Scientist | Histopathology t: 02 9845 3316 | f: 02 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: www.schn.health.nsw.gov.au m: 0425 314 267 Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 P Please consider the environment before printing this email. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Teri Johnson [tjohn...@gnf.org] Sent: Wednesday, 3 July 2013 3:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: HE staining question Hi Brett, I agree with the others, the storage in 70% ETOH is likely not the cause of the washed out appearance of the staining. * Did the lab that did the HE staining run a daily control? How did that look? Perhaps the tap water they are using went a little funny? Perhaps the depar xylene needs refreshing? The hematoxylin is used up? If all the other HEs done that day look good, then look further. * Look at the fixative, it is possible to get bad batches of that. It is also possible to cram a lot of tissue into a small space resulting in inadequate fixation. Or perhaps it was bloody and not changed to fresh fixative? Was one sample fixed at room temperature and the other fixed at 4 degrees C? Was the person doing the necropsy the same among animal batches? * Two things might help get better hematoxylin staining - I recall a journal article about using antigen retrieval pretreatment to improve HE staining on samples that had been stored in fixative for a prolonged period of time. That might help. Years ago, I also noticed that the hematoxylin counterstained PAS slides looked better than our HEs, so we put a bucket of 0.5% periodic acid as an oxidation step before hematoxylin. It needs to be rinsed well so there is no periodic acid carryover (that'll kill your hematoxylin!), but that might improve your staining. If you get this figured out, please let us know the cause and fix. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals
Re: [Histonet] Re: HE staining question
Oh by the way, the reason I did a Harris hematoxylin and eosin/phloxine on GMA (plastics) was because our pathologists at OHSU hated Gills and were used to seeing Harris hematoxylin on all of our regular surgical and autopsy slides. We only did GMA on kidney, liver, some GI and BM core bx's. I was just a plain ole histologist that wanted to please her pathologists! Anything can be accomplished if you are creative and innovative! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com From: Akemi Allison akemiat3...@yahoo.com To: Tony Reilly tony_rei...@health.qld.gov.au; Teri Johnson tjohn...@gnf.org; Linda Prasad (SCHN) linda.pra...@health.nsw.gov.au Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, July 3, 2013 4:54 PM Subject: Re: [Histonet] Re: HE staining question Tony: I actually was going to suggest this. You beat me to it! I used to do GMA on 1.5 microns sections and did a Harris hematoxylin and eosin/phloxine routinely (by the way, this was not recommended back in 1980. Gills hematoxylin was the norm!). To get the results I wanted, I would routinely oxidize the slides in 1% periodic acid and rince in running tap water before staining in hematoxylin. the staining was intensified and was beautiful! I came up with this because my PAS's with hematoxylin counterstains looked better than routine HE's. I figuired it was worth doing the GMA's routinely using 1% periodic acid since we didn't do that many. Funny how we stumble on our modifications! Science never sleeps!! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com From: Tony Reilly tony_rei...@health.qld.gov.au To: Teri Johnson tjohn...@gnf.org; Linda Prasad (SCHN) linda.pra...@health.nsw.gov.au Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, July 3, 2013 4:06 PM Subject: [Histonet] Re: HE staining question Hi Linda For a little more information (not an article) go to: http://www.ihcworld.com/royellis/problems/problem21.htm regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ Teri Johnson tjohn...@gnf.org 7/4/2013 12:37 am Hi Linda, There is no journal article, merely an observation on my part many years ago. Teri On Jul 2, 2013, at 10:05 PM, Linda Prasad (SCHN) linda.pra...@health.nsw.gov.au wrote: Hi Teri, Oxidising the slides in periodic acid prior to HE improves the HE. Would really appreciate if you could please send me the reference for that article. Thank you Teri. Linda Prasad, MSc, BSc Hospital Scientist | Histopathology t: 02 9845 3316 | f: 02 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: www.schn.health.nsw.gov.au m: 0425 314 267 Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 P Please consider the environment before printing this email. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Teri Johnson [tjohn...@gnf.org] Sent: Wednesday, 3 July 2013 3:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: HE staining question Hi Brett, I agree with the others, the storage in 70% ETOH is likely not the cause of the washed out appearance of the staining. * Did the lab that did the HE staining run a daily control? How did that look? Perhaps the tap water they are using went a little funny? Perhaps the depar xylene needs refreshing? The hematoxylin is used up? If all the other HEs done that day look good, then look further. * Look at the fixative, it is possible to get bad batches of that. It is also possible to cram a lot of tissue into a small space resulting in inadequate fixation. Or perhaps it was bloody and not changed to fresh fixative? Was one sample fixed at room temperature and the other fixed at 4 degrees C? Was the person doing the necropsy the same among animal batches? * Two things might help get better hematoxylin staining - I recall a journal article about using antigen retrieval pretreatment to improve HE staining on samples that had been stored in fixative for a prolonged period of time. That might help. Years ago, I also noticed that the hematoxylin counterstained PAS slides looked better than our HEs, so we put a bucket of 0.5% periodic acid as an oxidation step before hematoxylin. It needs to be rinsed well so
Fw: [Histonet] p40
Hey Jim: Just read the abstract you sent the link too. Once again congratulations on yet another accepted abstract! That was pretty much the jest of Davids' information and side-by-side images of p63 verses p40 on lung CA. Akemi Allison B.S., HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577 X117 Email: aalli...@montereygi.com From: Jim Burchette jburc...@gmail.com To: Akemi Allison akemiat3...@yahoo.com Cc: Taylor, Jean jtay...@meriter.com; ih...@googlegroups.com ih...@googlegroups.com Sent: Monday, May 13, 2013 3:44 PM Subject: Re: [Histonet] p40 Another application for p40: p40 (ΔNp63) and keratin 34βE12 provide greater diagnostic accuracy than p63 in the evaluation of small cell lung carcinoma in small biopsy samples. Butnor KJ, Burchette JL. Hum Pathol. 2013 Mar 22. doi:pii: S0046-8177(13)00044-0. 10.1016/j.humpath.2013.01.011. [Epub ahead of print] On Fri, May 10, 2013 at 6:35 PM, Akemi Allison akemiat3...@yahoo.com wrote: Biocare Medical. David Tacha just gave a a workshop on this at the CSH meeting last weekend. Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com From: Taylor, Jean jtay...@meriter.com To: 'ih...@googlegroups.com' ih...@googlegroups.com; 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Friday, May 10, 2013 10:53 AM Subject: [Histonet] p40 Hi everyone, I'm looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I'm wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven't found that a lot of companies that carry it. Any info would be helpful. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jim Burchette Fly Fishing Bum '((( ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] p40
Biocare Medical. David Tacha just gave a a workshop on this at the CSH meeting last weekend. Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com From: Taylor, Jean jtay...@meriter.com To: 'ih...@googlegroups.com' ih...@googlegroups.com; 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Friday, May 10, 2013 10:53 AM Subject: [Histonet] p40 Hi everyone, I'm looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I'm wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven't found that a lot of companies that carry it. Any info would be helpful. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Giardia controls in exchange for H-Pylori controls
Hello Everyone in Histoland! I would like to reach out to you to see if anyone out there would be willing to exchange some Giardia Controls for H-Pylori contols. Please let me know as soon as possible. My pathology director has requested me to start doing research on duodenum polyps to check for Giardia using a Giemsa method. Thank you in advance, Akemi Akemi Allison B.S., HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Medical Group 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (8310 375-3577 x 117 Email: aalli...@montereygi.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Attention GI facilities-(Giardia) Statistical Information on Duodenum polyps stained
Hello again everyone: The medical director of pathology at my facility asked to make an inquiry on Histonet regarding any statistical information of special staining on duodenum polyps for Giardia routinely. Do you have any statistics, and if so, do you have the percentages too? Any information would be greatly appreciated. Also, do you do these special stains on everyone of your duodenum polyps?We do not want to do IHC, but prefer special stains because of the cost factor. I used to do a Giemsa modification years ago, but he would like to know if there are any of you doing other special stains for Giardia. We are trying to avoid the more complicated silver stains such as Steiner or Warthin Starry. Thank you Akemi Akemi Allison B.S., HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Medical Group 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (8310 375-3577 x 117 Email: aalli...@montereygi.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: Minor clarification Attention GI facilities-(Giardia) Statistical Information on Duodenum polyps stained
Sorry, Just to make a minor clarification. Duodenal biopsies, not polypectomies. Thanks for all of your efforts! Akemi Allison B.S., HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Medical Group 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (8310 375-3577 x 117 Email: aalli...@montereygi.com Original Below The medical director of pathology at my facility asked to make an inquiry on Histonet regarding any statistical information of special staining on duodenum polyps for Giardia routinely. Do you have any statistics, and if so, do you have the percentages too? Any information would be greatly appreciated. Also, do you do these special stains on everyone of your duodenum polyps?We do not want to do IHC, but prefer special stains because of the cost factor. I used to do a Giemsa modification years ago, but he would like to know if there are any of you doing other special stains for Giardia. We are trying to avoid the more complicated silver stains such as Steiner or Warthin Starry. Thank you Akemi Akemi Allison B.S., HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Medical Group 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (8310 375-3577 x 117 Email: aalli...@montereygi.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Happy 79th Birthday Jules Elias
For those of you who were wondering what happened to Jules Elias. It's his 79th Birthday today! I just got an email from Jules telling me he and Renee just recently returned from a month long cruise to Tahiti and Hawaii. They still do a fair job on the dance floor. His music making has reached higher levels than he ever anticipated and his time is quite taken up by rehearsals and performances. He's thinking of giving a NSH seminar on the history of our profession starting with the days at the AFIP with Lee and Nick and going to where we are now as a recognized profession by both pathologists and other medical professionals. Akemi Allison, BS.,HT/HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Share room for NSH
Regarding NSH 40th Anniversary Meeting: I'm trying to cut costs to attend. Are any of my friends going to the NSH meeting that would like to share a room? If you are giving a workshop, I would be more than happy to compensate you with some sort of a stipend since you get your room paid for. Let me know. I am looking not only for workshop presenters. It would be nice to share a room with a person I know, but am willing to get to know new friends. It helps to reduce costs and get to go! Thanks, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Type I Water
It is recommended to use DI water for your water bath, especially when cutting tissues for IHC. Tap water has all kinds of bacteria, as well as potential chorine, rust, lead and other components which might have adverse affects to your histologic testing. Ethel Macrea from Ventana has presented an excellent workshop on water for use in the histology lab at NSH national and state meetings. If you have an opportunity i highly recommend attending As a good housekeeping practice, I always recommend cleaning your water baths with a soapy detergent after use, rinsing with tap water, drying residual water from the water bath and either turning upside down, or covering to prevent dust particles. Akemi Allison BS, HT(ASCP)HTL From: Sarah Dysart sdys...@mirnarx.com To: Nancy Schmitt nancy_schm...@pa-ucl.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Friday, February 15, 2013 6:38 AM Subject: [Histonet] RE: Type I Water I put tap water in my water bath and haven't had issues. Alternatively you could use DI water which would be a little cleaner... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, February 15, 2013 8:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Type I Water Clearly I sent this too quickly (early) - this is in regards to the water in our tissue float baths - does it HAVE to be Type I? Thanks Nancy Good Morning- I feel like I am racking up frequent flier miles on Histonet lately:) We are seeing bacteria in our type I. This requires us to obtain our Type I from another of our lab sites. Do I have any other options? Like tap water? Nancy Schmitt HT, MLT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Warning SCAM from a histonet Hi email
Hello All: Here we go again, I have been SCAMMED! It's been 4 years, but still disturbing! Yesterday I opened an email from a histonet subscriber with a subject of Hi. Not sure who the person was, but it stated MD. This morning I opening my emails and had numerous Mail Failures to my email address. They must have hijacked my addresses. This may happen to you! Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hi SCAM FROM Stephen Peters MD
FYI- The Scam came email came from Stephen Peters, MD. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Also Hi SCAM FROM enrri...@yahoo.com and kacly...@yahoo.com
That's because they Hi-Jacked my email address book and sent a scam in my name... I have over 200 MD's in my email address book so this makes me look bad, I did some back tracking and I also noticed I received the same SCAM from histonet address but didn't open. enrri...@yahoo.com and Lynch Kenia kacly...@yahoo.com Akemi Allison BS, HT(ASCP)HTL From: Horn, Hazel V hor...@archildrens.org To: 'Bitting, Angela K.' akbitt...@geisinger.edu; Akemi Allison akemiat3...@yahoo.com; Histonet Histonet@lists.utsouthwestern.edu Cc: Linda Margraf linda.marg...@childrens.com Sent: Wednesday, January 30, 2013 5:40 AM Subject: RE: [Histonet] Hi SCAM FROM Stephen Peters MD And another from Akema. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Wednesday, January 30, 2013 7:29 AM To: Akemi Allison; Histonet Cc: Linda Margraf Subject: RE: [Histonet] Hi SCAM FROM Stephen Peters MD There is another that appears that its from Gloria Limetti. Don't open the link. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, January 30, 2013 8:26 AM To: Histonet Cc: Linda Margraf Subject: [Histonet] Hi SCAM FROM Stephen Peters MD FYI- The Scam came email came from Stephen Peters, MD. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hi histonet
check out this page http://bit.ly/VmkCzS Akemi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histotechnician Salary 2
Tim: I have worked in California since 1998, both in the SF Bay area and LA. The cost of living is extremely high in these two areas compared to the rest of California, and certainly higher than most of the country. Working as a manager in clinical and bio-tech settings, I found it difficult to find a histologist that was well rounded in all aspects of histology and IHC labs. The histologists that were suitable to hit the ground running were compensated accordingly. The average pay range paid for a registered HT/HTL was $25.00 to $40.00 per hour. There are a few bio-tech companies in the SF Bay area that hire unregistered histo techs and pay them $15.00 an hour. They primarily cut control tissues and do the less complicated assignments, I also know of several clinical histology laboratories in LA and SF that hire unregistered histo tech's and pay them $15.00 to $20.00 an hour. You have to understand that we do not require registered histo techs to work in a lab as long as they have a registered supervisor. The quality of work is compromised because these techs do not have the knowledge of theory and practice, You pretty much get what you pay for. I was told a couple of years ago from a very good source that a recent graduate of a LA histology program who passed their HT registry with a year of experience was paid $25.00 an hour to start. Hope this helps. Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com From: Morken, Timothy timothy.mor...@ucsfmedctr.org To: Tim Wheelock twheel...@mclean.harvard.edu; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, December 19, 2012 12:14 PM Subject: RE: [Histonet] Histotechnician Salary 2 Tim, it depends on the going rate in your area. What does your hospital pay a general histotech? How about other hospitals in the area. I'll bet your personnel dept has that data. In California it can range from $15 to $35 per hour for histotechs with 5 years experience. The lower is in the rural areas and the higher in the San Francisco or LA areas. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Wednesday, December 19, 2012 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechnician Salary 2 Hi again all: I should have mentioned that the position calls for doing classical neuropathology stains, but not IHC. We have our immunostains done by another lab. Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 88305TC starting to hit the fan...
Hello: You stated 88305TC starting to hit the fan... Would you care to explain some of the details with us. I work in a private GI lab which contracts our pathology PC mortion to local pathologists. One of which is our medical director, as well as the medical director for a local community hospital. They come to our facility to do the microscopic portion of their work. Regards, Akemi Akemi Allison, BS, HT(ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577 X117 Email: aalli...@montereygi.com From: Michael Kent mk...@dermpathlab.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, December 18, 2012 1:25 PM Subject: RE: [Histonet] 88305TC starting to hit the fan... Fellow Histoneters, Please feel free to contact me if your lab is in trouble from these cuts. We may be able to help. Also- we are hiring a Histotech in the Troy Michigan area, if you know anyone interested. Best and Happy Holidays, Michael Kent, MS, PhD Translational Scientist Dermatopathology Laboratory of Central States Adjunct Assistant Professor of Dermatology Wright State University Boonshoft School of Medicine 7835 Paragon Rd. Dayton OH 45459 (937) 436-4152 http://www.dermpathlab.com/ Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pam Plumlee wants to share new pictures with you :)
Has anyone else received this invite??? Not sure if it is legit? Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com From: Pam Plumlee paw...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Friday, October 12, 2012 5:50 PM Subject: [Histonet] Pam Plumlee wants to share new pictures with you :) Pam Plumlee has added you as a friend on Zoosk. Is Pam Plumlee your friend? [1]Yes [2]No This message was sent by a Zoosk user who entered your email address. If you'd prefer not to receive emails when other people send you emails through Zoosk, [3]click here You have received this message at the email address: [4]histonet@lists.utsouthwestern.edu Copyright © 2007-2012 Zoosk, 989 Market St, San Francisco, CA 94103 USA. [5]Privacy Policy Zoosk References 1. https://www.zoosk.com/signup/friend?invite-token=018fcd224966f34314859f1805621fbbt_ctr=IN_70_00_en_XX_US_02_S_20121013_00_Efrom=find-friends-yes-IN_70_00_en_XX_US_02_S_20121013_00_E 2. https://www.zoosk.com/signup/friend?t_ctr=IN_70_00_en_XX_US_02_S_20121013_00_Efrom=find-friends-no-IN_70_00_en_XX_US_02_S_20121013_00_E 3. https://www.zoosk.com/optout.php?with=histonet%40lists.utsouthwestern.edukey=849507f838bb201b7ea4db2ee33a5222from=email_invite 4. mailto:histonet@lists.utsouthwestern.edu 5. http://www.zoosk.com/privacy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Why am i getting zoosk invites?
I received the same why Akemi Allison-Tacha BS, HT(ASCP)HTL E-Mail: akemiat3...@yahoo.com From: Erinandjesseconley erinandjessecon...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Friday, October 12, 2012 7:46 PM Subject: [Histonet] Why am i getting zoosk invites? I have no interest in a dating service. Why would histonet be affiliates with this site? Sent from my Samsung Epic™ 4G Touch ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pam Plumlee wants to share new pictures with you :)
Take me off this list! We are not a dating service! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com From: Pam Plumlee paw...@yahoo.com To: histonet@lists.utsouthwestern.edu Sent: Friday, October 12, 2012 5:50 PM Subject: [Histonet] Pam Plumlee wants to share new pictures with you :) Pam Plumlee has added you as a friend on Zoosk. Is Pam Plumlee your friend? [1]Yes [2]No This message was sent by a Zoosk user who entered your email address. If you'd prefer not to receive emails when other people send you emails through Zoosk, [3]click here You have received this message at the email address: [4]histonet@lists.utsouthwestern.edu Copyright © 2007-2012 Zoosk, 989 Market St, San Francisco, CA 94103 USA. [5]Privacy Policy Zoosk References 1. https://www.zoosk.com/signup/friend?invite-token=018fcd224966f34314859f1805621fbbt_ctr=IN_70_00_en_XX_US_02_S_20121013_00_Efrom=find-friends-yes-IN_70_00_en_XX_US_02_S_20121013_00_E 2. https://www.zoosk.com/signup/friend?t_ctr=IN_70_00_en_XX_US_02_S_20121013_00_Efrom=find-friends-no-IN_70_00_en_XX_US_02_S_20121013_00_E 3. https://www.zoosk.com/optout.php?with=histonet%40lists.utsouthwestern.edukey=849507f838bb201b7ea4db2ee33a5222from=email_invite 4. mailto:histonet@lists.utsouthwestern.edu 5. http://www.zoosk.com/privacy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray
Well said Rene! In my 40 plus years as a supervisor, manager, technical support manager, and consultant; I have come across a broad spectrum of experienced and inexperienced histologists. Their knowledge base may be limited to their own experiences, or lack of it. A good supervisor or manager will be open to alternative methods of techniques. The mind is like a parachute; keep it open! Akemi Allison BS, HT(ASCP)HTL From: Rene J Buesa rjbu...@yahoo.com To: Jenny Vega histotech...@gmail.com; joelle weaver joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Sunday, September 30, 2012 7:17 AM Subject: Re: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray Jenny: There is a saying that life is too short to drink cheap wine. In the same way life is too short to be frustrated daily working at a place where work is like a daily uphill battle. For what you describe you know your trade. Start looking for another place although do not expect that your ideas will always be well received. Older, trained on the job, and with lots of experiences supervisors usually are not very open to suggestions, especially when those ideas conflict with what they are used to do because they do not know the scientific basis of what they are doing. The less open to suggestions a person is, the more ignorant they are likely to be. René J. From: Jenny Vega histotech...@gmail.com To: joelle weaver joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu Sent: Saturday, September 29, 2012 6:26 PM Subject: Re: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray Thanks everybody for your answers. I cant respond them all but I concluded that the best way to get good sections is too chill the blocks on ice because I agree that it facilitates the process. I really don't understand why my supervisor depends so much on freezing sprays to cut and the pathologist has never complained about artifacts caused by them but I do believe that they are present because I have seen them getting formed. It makes sectioning difficult because you try to get sections free of holes and that contributes to the problem. At my lab is the same thing. My supervisor is in charge of the embedding and she just use the ice only for hardening the paraffin block. We don't have a standard embedding center with cold plate. Since is a small lab we just have a heating plate where we handle the specimens and place them in the molds and we cool them on ice trays. After they are removed from the molds they are placed on the counter in numerical order and they reach room temperature and get warm. I do think that if they get cold and moistened since the beginning it can facilitate the sectioning process except for certain tissues that are not well processed. In my lab I change the reagents in the tissue processor weekly but they get dirty too quickly because we processes a lot of breast and colon tissue so is hard to get perfectly processed tissue daily. We are under a tight budget and we can't waste materials too quickly. This situation with my supervisor has caused me a lot of frustration. I have noticed that every tech has their own method to do things but unfortunately there are people who are not receptive to new ideas and they immediately criticize and say you are wrong specially if you are young and you have recently started your career. I have though on several occasions that all I learned in histotech school have been worthless because everybody in the lab does things differently and this has made me question my ability of being a good tech because I have experienced difficulty using their microtomy technique but I have realized that I am not wrong. Another issue was the use of microtome blades. Since I started my supervisor has told me to use the minimum amount of blades as possible because we are under a tight budget but I have noticed that some of those blades are of poor quality and since we cut a lot of tissues that are hard, calcified or have sutures they wear the blades too quickly. It's hard to cut many blocks using only one blade that you use to trim and section. I have realized that I am successful obtaining good sections when I chill the blocks on ice for a couple of minutes and change the blade immediately after encountering difficulties with a block. It's not worth to sacrifice the quality of the samples just to be cheap and save some money. My supervisor is a great person and tech with a lot of experience but she is not very open to new ideas. Her demands to save money can be unrealistic when the correct technique is not being used . With her technique of course you are going to waste excess of freezing spray and blades but she doesn't understand this. Perhaps I need a change of environment which is unfortunate but is difficult to work like this. Thanks everyone
[Histonet] Akemi's Email contacts Hi-Jacked
Hello All: Sorry, but it looks like someone hi-jacked my email addresses Not sure how this happened last night. Could have been from my LinkedIn, but I changed my password 2 days ago. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SPAM Sent-Akemi's Email Addresses Hi-Jacked
Hello All: SPAM Sent! Sorry, but it looks like someone hi-jacked my email contact addresses I have a MAC, but it could have been from my LinkedIn or my Facebook. I changed my password 2 days ago on my LinkedIn after the notification of a security breach. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] QA
10% Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Feb 13, 2012, at 11:51 AM, Amber McKenzie wrote: What % QA do most pathologists do internally? I'm trying to update my AP Manual. My last CLIA inspection, they told me to put in my AP Manual that it is done and get a binder labeled discrepancies. If there are any... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Precipitate
I agree with Tim, but I am curious why tap water is being used to make the 70% alcohol instead of DI water. Tap water has been known to have all kinds of contaminates. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Jan 27, 2012, at 6:23 AM, Morken, Timothy wrote: Matt, we have seen that in one of our VIP5's and we do weekly hot- water rinse of the first 4 stations. It is the oldest processor, at about 7 years. That processor had frequent pump-in pump-out errors randomly in the first 3 stations. Finally the service tech decided to get to the bottom of it and he found the steel tubing clogged with sand. It took a whole day to clean it all out. It did not look like formalin salts, but did look like the kind of deposits that you see with hard water in pipes. Since then, no problems at all. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Matt Mincer Sent: Thursday, January 26, 2012 5:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Precipitate Hey Histonet, We have a client who is having an odd problem with their processor. They are getting sandy clogs in station 3. The original thought was that it was formalin salts but the texture and color was wrong. Also, station 3 is 70% which should be weak enough. One of the techs mentioned in passing that the water quality in their town was really bad. I think that the problem is that, like formalin, the alcohol is causing dissolved minerals to be released from the tap water they use to mix their 70%. Has anyone seen this before or am I chasing a harebrained theory? Any thoughts would be greatly appreciated. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Looking for a used Biocare Decloaker
Why don't you ask your local Biocare rep if they have any Demo's available for sale. They used to have some available for sale. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Dec 16, 2011, at 4:10 PM, Sowmya Kedarnath wrote: Hello Histonetters! Anybody interested in selling a used Biocare Decloaker kindly email me with your contact details.Would greatly appreciate the help. Best Sowmya Kedarnath ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP meeting in Carmel, CA November 4-5
Hi All: Just wondering if any of you in histoland are going to the CAP meeting in Carmel at the La Playa in November. Let me know, I am living in Carmel, and would love to connect. Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tumors tumors everywhere
Sarah, I am curious; have you tried contacting the BioCare research tech support reps? They are very helpful regarding their products and trouble-shooting any issues that may arise. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 28, 2011, at 1:16 PM, sgoe...@mirnarx.com sgoe...@mirnarx.com wrote: So I am staining tumors that were implanted as cells subdermally into mice. The cells are human. I am trying to do Caspase staining on these tumors. The primary is an anti-human rabbit polyclonal, and I am using a polymer (Biocare Mach3) in lieu of the secondary antibody. The background is through the roof!! Could the reason be that the tumor was grown in a mouse and is having cross reactivity somehow? What species antibody should I be using instead? All my mouse monoclonal antibodies work perfect on the tissue, it's this stupid rabbit polyclonal!! I am blocking endogenous enzymes (peroxidase etc., DAKO), avidin and biotin (just to see if that would help...it didn't), and protein block (it's literally an hour worth of blocking!!), developing with DAB (Dako) and hematoxylin counterstain. I am so confused as how to get this to work! Also, it isn't just this particular antibody it is any rabbit polyclonal I have tried. Could it be the polymer? It is the one that Biocare suggested? HELP!! Thanks in advance =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] endogenous peroxidases
Make sure you use a fairly fresh bottle of H2O2. (no older than 6 months). The stock bottle degrades after it is opened. I have seen people using an older bottle of H2O2 and the results are sub-optimal. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 22, 2011, at 10:27 AM, Hobbs, Carl wrote: Gimme 5! Block before or after Ag retrieval. If it's Pwax sections make a 1/10 dilution of 100vols ( ~30%) H2O2 in Dist.water. Block for 15mins. Methanol is not neccessary...imho. Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus London SE1 1UL Tel: 020 78486813 Fax: 020 78486816 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slide drying ovens
There are many so called convection ovens out there that are gravity convection. Biocare has a great convection oven that has an internal fan, and can be set with several different programs. You can set it for IHC at the end of the day and it automatically stops and is ready for you in the AM when you come in. It has a small footprint. It is not cheap, but well worth it. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 19, 2011, at 10:13 AM, Bell, Mandy wrote: I'm looking for recommendations for a new slide drying oven. Something relatively small, with convection. Any input would be appreciated. Thanks ! Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 3, 000 Breast Cancer Specimens from Quebec Breast Cancer being retested
PhenoPath Laboratories Retests Nearly 3,000 Breast Cancer Specimens from Quebec Breast Cancer specimens which may have been misdiagnosed. In the re-analysis, results of which were just announced, a total of 87 women were found to have received a false negative result from the original pathology laboratory tests in Quebec. Of this number, 39 were required to alter their treatment and among them five women died. Minister of Health Yves Bolduc said it was impossible to determine whether the women died as a result of being improperly treated. Check out this link. http://www.ihc.co/a824362-seattle-based- phenopath-laboratories-retests-nearly-.cfm Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Standardization??? 3, 000 Breast Cancer Specimens from Quebec Breast Cancer being retested
From what I have experienced in my observations of various labs; numerous labs do not follow proper standardization practices which are set by FDA guidelines for Her2-neu. Prognostic markers are particularly susceptible to heat. Baking slides in excess temperatures (over 62 degrees C) for prolonged lengths of time can also drop the antigenicity of the test tissues a full grade. There has been documentation of this through publications. Temperatures MUST be monotored and recorded. The Antigen Retrieval Process is very important and QC measures MUST be followed. Internal temperature and pressure MUST be met and recorded. QC monitor strips will record and show any irregularities in this step. If the pressure and temp has not been met, the test should be repeated. A lot of IHC techs do not monitor this process. Unfortunately, internal laboratory standardization can vary from shift to shift. Just think about non- standardization practices throughout the country because of under- education and strict adherence to established guidelines. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 19, 2011, at 10:36 AM, Akemi Allison wrote: PhenoPath Laboratories Retests Nearly 3,000 Breast Cancer Specimens from Quebec Breast Cancer specimens which may have been misdiagnosed. In the re-analysis, results of which were just announced, a total of 87 women were found to have received a false negative result from the original pathology laboratory tests in Quebec. Of this number, 39 were required to alter their treatment and among them five women died. Minister of Health Yves Bolduc said it was impossible to determine whether the women died as a result of being improperly treated. Check out this link. http://www.ihc.co/ a824362-seattle-based-phenopath-laboratories-retests-nearly-.cfm Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rita Humphrey
Hello Histoland! Does anyone know how to get in touch with Rita Humphrey, previously with Childrens Hospital, Birmingham, AL? I lost touch with her after she left Childrens and would like to re-connect. Thanks in advance, Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GREAT B5 substitute
Hi All, I used to use B5, Zenker's and Helly's for years on LN and BM biopsies. I wanted to stop using mercury in our department, but didn't want to compromise the quality of the results we were getting. I was interested in using fixatives and decalcification products that would not effect IHC results. At the suggestion of Dr. Elaine Jaffe, Chief Hematopathologist from NIH, I tried 2 products from BBC Biochemical. I did parallel studies on bilateral BM bx's using B•Plus Fixative™ and RapidCal•Immuno™, and then B5 fixative and EDTA decal solution, and then Zenker's fixative. We then did parallel studies for IHC. We ran a panel of Ab's on specimens which were treated with each of the solutions. The pathologists concluded that B•Plus Fixative™ produces superb fixation for hematopoietic tissues and the RapidCal•Immuno™ does not interfere with IHC staining. In some cases, the results on the IHC's were better on some of the Ab's using these products too. It only takes 2-3 hours of fixation time for BM biopsies. After prefixing, they can go directly into RapidCal•Immuno™ for 45 minutes to 1 hour, washed in running water for 5 minutes, and then continue to be processed as normal starting off in 10% formalin. Just a note: This product is a rapid decalcifier designed to preserve immunogenicity at a maximum while accomplishing rapid decalcification. It is designed specifically for bone marrow biopsies and LN biopsies. By the way, I am not on BBC's payroll. I am always on a quest to improve patient care! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] bone marrow biopsy decals
Hi Diana, I recently made a histonet posting Great B5 substitute which also addresses BM decal. Since there are a number of companies who sell decal solutions with similar names, I am not sure you tried the products from BBC Chemical. It is best to use both products together. My post goes into my findings. Regards, Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Mar 24, 2011, at 10:00 AM, Diana Martinez-Longoria wrote: Our pathologist wants to know why Formical-4 is the best decal for bone marrow biopsies, since we heard about in Histonet? I have experimented with four different decals: Rapid-Immuno, Formical-4, Nitric Acid, and HCL\Formic Acid and we are still not sure which will be the best decal. Also we a decal that will minimize the leeching out of iron. If any out there has any suggest, please let me know. Thanks in advance! Diana Martinez-Longoria Histotechnician HT(ASCP)CM El Centro Regional Medical Center 1415 Ross Ave El Centro CA 92243 (760) 339-7267 (760)482-5365 fax dmlongo...@ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e- mail and its attachments. Â ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GREAT B5 substitute
Having worked and developed products in the Biotech arena, not all products are disclosed. If they are, you lose the competitive edge. Here is the link to the the MSDS and information BCC discloses; http://www.bbcus.com/uploads/files/BPlusFix_MSDS.pdf Section 2. Composition and Information on Ingredients Name CAS# % by Weight Exposure Limits 1) Water 2) Formaldehyde 37% 3) Zinc Salts 4) Selected Buffers 7732-18-5 50-00-0 Not Available Not Available 25% 0.5% - 1% Not Available Not Available OSHA PEL 0.75ppm TWA Not Available Not Available Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Mar 24, 2011, at 12:28 PM, Johnson, Nacaela wrote: Are the contents of the B Plus Fixative proprietary? Is it an AZF type of fixative? Did you try any ISH on the test runs? Thanks, Nacaela Johnson, HTL (ASCP) Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Thursday, March 24, 2011 12:08 PM To: histonet Subject: [Histonet] GREAT B5 substitute Hi All, I used to use B5, Zenker's and Helly's for years on LN and BM biopsies. I wanted to stop using mercury in our department, but didn't want to compromise the quality of the results we were getting. I was interested in using fixatives and decalcification products that would not effect IHC results. At the suggestion of Dr. Elaine Jaffe, Chief Hematopathologist from NIH, I tried 2 products from BBC Biochemical. I did parallel studies on bilateral BM bx's using B*Plus Fixative(tm) and RapidCal*Immuno(tm), and then B5 fixative and EDTA decal solution, and then Zenker's fixative. We then did parallel studies for IHC. We ran a panel of Ab's on specimens which were treated with each of the solutions. The pathologists concluded that B*Plus Fixative(tm) produces superb fixation for hematopoietic tissues and the RapidCal*Immuno(tm) does not interfere with IHC staining. In some cases, the results on the IHC's were better on some of the Ab's using these products too. It only takes 2-3 hours of fixation time for BM biopsies. After prefixing, they can go directly into RapidCal*Immuno(tm) for 45 minutes to 1 hour, washed in running water for 5 minutes, and then continue to be processed as normal starting off in 10% formalin. Just a note: This product is a rapid decalcifier designed to preserve immunogenicity at a maximum while accomplishing rapid decalcification. It is designed specifically for bone marrow biopsies and LN biopsies. By the way, I am not on BBC's payroll. I am always on a quest to improve patient care! Akemi Allison BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alcohol Xylene recyclers
Hi Everyone in histoland! I would like to get your feedback on which alcohol / xylene recyling units you prefer. I would like information regarding purity of end product, cost, size of footprint, and relyability. We currently do not have a recycling unit, and I have been requested to gather information. Thank you, Akemi Allison BS, HT(ASCP)HTL E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP
Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
