[Histonet] Microscope slide storage cabinets?

[Cooper, Brian via Histonet]

Happy Friday Histonet!

What is everyone using for long term microscope slide storage these days?  Our 
custom slide drawers in electronic shelving units are almost at capacity, and I 
need to move a LOT of old slides offsite.   All of our REALLY old slides are in 
"Technicon" style metal drawers.  Is there anything better on the market?  We 
won't be making cardboard boxes . . .

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Premade Eosin

[Cooper, Brian via Histonet]

When we're not using our Ventana HE600 (their proprietary products), we use 
Eosin Y 515 from Leica.  Our frozen section room uses Protocol Eosin-Y from 
Fisher. 

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Piche, Jessica via Histonet  
Sent: Tuesday, April 2, 2024 9:12 AM
To: Histonet 
Subject: [Histonet] Premade Eosin (EXTERNAL EMAIL)

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Good Afternoon,

I hope everyone is having a great week so far!

Can anyone share what pre-made eosin brands they are using and recommend? We 
use Eosin Y. We make it ourselves and want to look into pre-made.

Thank you in advance. Have a great day.

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
Histology Team Leader
203-573-7167
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Re: [Histonet] Bone samples

[Cooper, Brian via Histonet]

Embed the bones diagonally in your molds if you're able (size depending) as 
this will allow for the greatest amount of paraffin support.  Trim them very 
slowly, and if need be, place the blocks into the same "slow decal" solution 
for additional amount of time sufficient to enable better sectioning.  Start 
checking in half hour or 45 minute intervals; rinse the blocks well in running 
water and attempt to section.  If they're not ready, back into decal solution 
they go.  

I feel that very wet ice helps to facilitate sectioning better than ice that is 
drier and fresh out of the freezer.  Just be sure to blot the face of the block 
with gauze before attempting to cut.  

There's my two cents!

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Chakib Boussahmain via Histonet  
Sent: Wednesday, January 24, 2024 2:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone samples (EXTERNAL EMAIL)

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Hi guys,

 I hope this messagefinds you well. I am currently working on a study involving 
bone samples thathave been treated with slow decal and embedded in paraffin. I 
am facingchallenges in obtaining nice sections, and I was wondering if you 
could providesome guidance or recommendations.

Thank you in advance for your help!
Chakib
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[Histonet] We were introduced to an awesome new product!

[Cooper, Brian via Histonet]

Good afternoon HistoNet!

You know that product that many of us use to skim our flotation baths in 
between cutting blocks?  You know, those individual sheets in the green box?  
Well, for years now, I for one have been frustrated when say, a white-pages 
sized chunk of them come out when I go to grab one!  (I know, I dated myself 
there).  Or how the next sheet will retract inside the box, and then you have 
to surgically extract it to get on with it.  Or how this product tears, and 
you're forced to skim your bath with only a fragment?  It's been in every lab 
I've ever worked in!

If you get a chance to try them, Tech-Wipe specialty task wipers are such an 
amazing upgrade!  We have no financial gain here, I'd never even heard of this 
product until a few weeks ago.  None of the issues I mentioned above have 
happened!  Every one of our crew has told me how much they love this new 
product!  These have a little bit of elasticity to them, and that seemingly 
prevents tears.  And they only come out one at a time!  Ask your consumables 
rep if they carry it!  I never thought I'd write an email about skimming a 
waterbath; it's such a mundane task we've done millions of times as Histotechs. 
 But here you go!

Happy Tuesday!

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Microwave Processing

[Cooper, Brian via Histonet]

They may make a combo UPS/line conditioner.  That would be ideal.  Line 
conditioners stabilize and protect against power spikes/surges.

Thanks, 

Brian

-Original Message-
From: Campbell, Tasha  
Sent: Wednesday, November 8, 2023 12:40 PM
To: Cooper, Brian 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microwave Processing (EXTERNAL EMAIL)

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What does a line conditioner do? The company was bought out recently and 
basically has no employees so I have no one to help me. 

Tasha Campbell
Sent from my iPhone

> On Nov 8, 2023, at 3:36 PM, Cooper, Brian  wrote:
> 
> Hi Tasha,
> 
> Maybe look into getting a line conditioner for your tissue processors.  Your 
> tissue processor vendor may have some suggestions.
> 
> Thanks,
> 
> Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
> Pathology and Laboratory Medicine Children's Hospital Los Angeles
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> Ph: 323.361.3357
> bcoo...@chla.usc.edu
> 
> -Original Message-
> From: Campbell, Tasha via Histonet 
> Sent: Wednesday, November 8, 2023 11:44 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL)
> 
> CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside 
> CHLA. Do not open attachments, click on links, or respond unless you expected 
> this message and recognize the email address: 
> histonet-boun...@lists.utsouthwestern.edu.
> 
> Is there anyone that is super familiar with microwave processing? I really 
> need some help.   I am having trouble with my tissue and I think it's the 
> processing.  I don't know what else it would be.  We moved to a new building 
> so I don't know if the power could be stronger and its changing the way it 
> processes or what.  I have tried messing with the program times and temps but 
> I cannot figure it out.  My blocks keep having what looks like knife lines 
> but it's not the microtomes. The only change has been moving into a brand new 
> building.  Any help would be appreciated!  Thanks.
> 
> 
> Tasha Campbell, B.S., HTL (ASCP)
> Frederick Gastroenterology Associates
> 7109 Guilford Dr. Suite 300
> Frederick, MD 21704
> 301-695-6800 ext. 144
> 
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Re: [Histonet] Microwave Processing

[Cooper, Brian via Histonet]

Hi Tasha, 

Maybe look into getting a line conditioner for your tissue processors.  Your 
tissue processor vendor may have some suggestions.

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Campbell, Tasha via Histonet  
Sent: Wednesday, November 8, 2023 11:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL)

CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside 
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this message and recognize the email address: 
histonet-boun...@lists.utsouthwestern.edu.

Is there anyone that is super familiar with microwave processing? I really need 
some help.   I am having trouble with my tissue and I think it's the 
processing.  I don't know what else it would be.  We moved to a new building so 
I don't know if the power could be stronger and its changing the way it 
processes or what.  I have tried messing with the program times and temps but I 
cannot figure it out.  My blocks keep having what looks like knife lines but 
it's not the microtomes. The only change has been moving into a brand new 
building.  Any help would be appreciated!  Thanks.


Tasha Campbell, B.S., HTL (ASCP)
Frederick Gastroenterology Associates
7109 Guilford Dr. Suite 300
Frederick, MD 21704
301-695-6800 ext. 144

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Re: [Histonet] Formalin pH

[Cooper, Brian via Histonet]

Ask your vendor to send a certificate of analysis that includes the pH. That's 
what we did and now they come with each lot. We do have to bug them for them 
occasionally.

I'm betting your vendor has at least heard about this from other customers so 
it should be on their radar at the very least.


Thanks,



Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor

Department of Pathology and Laboratory Medicine

Children's Hospital Los Angeles

4650 Sunset Blvd MS#43- Los Angeles, CA 90027

Ph: 323.361.3357

bcoo...@chla.usc.edu


From: Paula Sicurello via Histonet 
Sent: Tuesday, September 12, 2023 7:12:05 PM
To: HistoNet 
Subject: [Histonet] Formalin pH (EXTERNAL EMAIL)

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Hello Histoteckies,
What are y'all doing regarding the CAP requirement to monitor the pH of 
formalin?
We buy tons and tons of the 5 gallon cubitainers and we are still debating over 
how to check the pH.

Looking forward to your replies.
Toodles!
Sincerely,

Paula Sicurello
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Re: [Histonet] Leica H 600

[Cooper, Brian via Histonet]

The Ventana HE600 is an awesome machine. You can't use xylene to remove the 
coverslips, you need to heat them on a hot plate to soften the glue, and then 
the slips just come right off.

I used other dip and dunk stainers in the past and I was skeptical at first 
too. Those concerns have been put to rest! The machine requires no manual user 
maintenance (PM service is of course required) or filtering of reagents. You 
can't put expired reagents on the machine, nor can you put reagents in the 
wrong place. The machine performs a cleaning cycle every evening, so we 
literally do nothing on this machine except press start.

No machine is completely perfect. Sometimes staining modules leak or fail. This 
machine has 3 separate staining modules so you can always disable the troubled 
module and the machine will still work. It's been very rare that our 
coverslipper has failed.

It's certainly not the cheapest stainer on the market, but I've told people who 
come to our lab that the machine is like another employee! It just works and 
works and works. I'll be happy to answer any further questions.


Thanks,



Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor

Department of Pathology and Laboratory Medicine

Children's Hospital Los Angeles

4650 Sunset Blvd MS#43- Los Angeles, CA 90027

Ph: 323.361.3357

bcoo...@chla.usc.edu


From: O'Neil, Beth via Histonet 
Sent: Wednesday, August 23, 2023 5:28:11 AM
To: Histonet 
Subject: [Histonet] Leica H 600 (EXTERNAL EMAIL)

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I would appreciate any feedback from anyone with experience using the Leica H 
600. I recently attended a demo of the unit and we are still hung up on the 
large slide tray it uses instead of the traditional slide racks.   I also spoke 
with another user who indicated that if you need to re-coverslip a slide it is 
very difficult to remove the existing coverslip and when you do the hematoxylin 
is removed from the stain ???  We already know we will need two units to handle 
our workload of approximately 900 H  Any information would be greatly 
appreciated.

Beth O'Neil
beth.one...@wvumedicine.org
West Virginia University, JW Ruby Memorial Hospital
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Re: [Histonet] Tissue Processor Schedule Validations

[Cooper, Brian via Histonet]

WOW!  I came back from lunch and I have seven responses already!  Everyone said 
the same thing, which was my game plan, take punches from larger samples!  
Happy Friday Eve everyone!   

Thanks so much Histonet!  

Brian

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Thursday, March 23, 2023 11:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processor Schedule Validations (EXTERNAL EMAIL)

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Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of 
Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>

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[Histonet] Tissue Processor Schedule Validations

[Cooper, Brian via Histonet]

Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming 
months, and I'm curious how people have validated small tissue processing 
protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier 
to do because we can readily gross duplicate sections. Obviously we can't adopt 
this approach for smaller samples because they're entirely submitted.  I have a 
game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Tracking of blocks onto processors

[Cooper, Brian via Histonet]

Hi Chris, 

At our institution, we're currently utilizing CoPath Automatic Barcoding and 
Tracking (AB).  Similar to Vantage, each step of the workload is tracked from 
accessioning → grossing → embedding→ microtomy→ staining→ QC (or case assembly) 
and then delivery to pathologist. For every cassette that grossed by our PA's 
the day before, that information shows up on an automatically printed report 
for our techs to verify that the actual block exists the next day (when they 
are embedded and cut) or being accounted for at the time of filing.  At 10 AM, 
a 2nd report will print highlighting any block that has yet to be cut (these 
blocks will either be in the grossed or embedded status).  Usually, this final 
report is simply a blank page, because we typically have all of our routine 
workload cut before this time.  If anything IS present on this report, it 
indicates that there is a cassette we didn't embed, have yet to cut, or worst 
case scenario, lost.  This report is basically a red flag warning--it's a way 
to tell if anything is missing, and that you'd better locate that missing 
sample ASAP.

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Willis, Donna G via Histonet  
Sent: Wednesday, March 22, 2023 5:29 AM
To: Hagon, Christopher (Health) ; 
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tracking of blocks onto processors (EXTERNAL EMAIL)

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Chris,
We have been tracking blocks since we went live with Vantage over 10 years ago. 
 Roche (Ventana then) built a custom program for us to use at that time because 
they had not yet implemented it into Vantage.  In the past 2 years Roche has 
updated their software to allow documenting this information.  There are 
several options of how the scanning is performed.  For our lab we scan before 
we place the block into the formalin holding tanks, right after the 
PA/Resident/Grossing Tech have placed the tissue into the block.  I prefer it 
done there because I do not want my technicians exposed an extra time to 
formalin if the scanning is done right before the baskets are placed on the 
processors.  We have a manual log that is used to document the time the 
cassette are placed in and taken out of the processors.  We also track our 
blocks and slides into storage.  I hope this helps.  

Donna Willis
Anatomic Pathology Manager
Baylor Scott Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



-Original Message-
From: Hagon, Christopher (Health) via Histonet 

Sent: Tuesday, March 21, 2023 5:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: {EXTERNAL} [Histonet] Tracking of blocks onto processors


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UNOFFICIAL

Hi Histonetters,

Just wondering if or how others are tracking/scanning racks of blocks onto 
processors. In this day and age of traceability with barcodes, we scan each 
block in each rack before loading onto a processor and recording which 
processor that rack went on to. Has anyone got a more efficient way of doing 
this? Assigning at grossing/cut-up is an option, but is an extra step for the 
PA/registrar and relies on knowing which processor is in use that day.

Looking in the crystal ball, will next gen processors automatically scan blocks 
as the first step? Load blocks, start the run and it produces a report of the 
blocks on that run? Interested to hear peoples thoughts.

Cheers,

Chris Hagon - Pathology
Digital Solutions Division, ACT Health Directorate


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Re: [Histonet] Special Stainers

[Cooper, Brian via Histonet]

Agilent-Artisan stainers are amazing.  You can search old Histonet posts with 
my explanation as to why! I once had a lot to say about this. 

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Kara, Phillip via Histonet  
Sent: Wednesday, February 22, 2023 11:05 AM
To: Eddie Martin via Histonet 
Subject: [Histonet] Special Stainers (EXTERNAL EMAIL)

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So I am working for a brand new lab that has been open since September. We 
currently perform all our specials by hand (roughly 7 stains; 3 silvers) and 
our numbers are starting to shoot up big time on them.
Any recommendations on special stainers and kits that go with them? We are a 
Sakura flag ship lab but we are open to other equipment if the cost is good and 
the results are great.

Phillip Kara BS, HTL(ASCP) : Lead Histology Tech Wise Pathology LLC
609 Medical Center Dr.
Decatur TX. 76234
Office: 940-626-0120
pk...@wisehealthsystems.com

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Re: [Histonet] destain for IHC

[Cooper, Brian via Histonet]

We used to do this all the time in my old reference lab.


Thanks,



Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor

Department of Pathology and Laboratory Medicine

Children's Hospital Los Angeles

4650 Sunset Blvd MS#43- Los Angeles, CA 90027

Ph: 323.361.3357

bcoo...@chla.usc.edu


From: Nancy Schmitt via Histonet 
Sent: Wednesday, February 8, 2023 9:29:13 AM
To: Histonet 
Subject: [Histonet] destain for IHC (EXTERNAL EMAIL)

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Hello-

Has anyone had success destaining an H and then running IHC on the same slide?

Thanks!

Nancy


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Re: [Histonet] CAP regulatory question

[Cooper, Brian via Histonet]

Hi Jessica, 

That's the way we read it here, but in most cases we have probably more like 3 
years-worth. 

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

Thanks, 

Brian

-Original Message-
From: Piche, Jessica via Histonet  
Sent: Thursday, February 2, 2023 9:13 AM
To: Histonet 
Subject: [Histonet] CAP regulatory question (EXTERNAL EMAIL)

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Good Afternoon,

Do we only have to keep lot to lot comparisons for immunohistochemistry and 
special stains for 2 years?

I hope everyone is having a good day.

Thank you.

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
Histology Team Leader
203-573-7167
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Re: [Histonet] Temperature Monitoring System

[Cooper, Brian via Histonet]

We use Isensix here, and I really like the system.  It's not exactly "wireless" 
because each unit is hardwired (with AA battery backup) into a CP unit which 
transmits relays data back to an Access Point (also hard wired), which in turn 
relays the information to the Control Center software. When a unit goes out of 
range, the system can email designated individuals or page specific numbers, 
and has backup notifications to designated individuals if the primary contact 
doesn't respond within a designated number of notifications sent.   They are 
"wireless" in the sense that there aren't wires running through ceilings back 
to a single central location, but there are certainly visible wires wherever a 
CP or AP is located.  We monitor refrigerators, freezers, room temp and 
humidity in countless sections of the pathology lab, and even elsewhere in the 
hospital here.  

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Matthew D. Roark via Histonet  
Sent: Wednesday, January 18, 2023 8:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperature Monitoring System (EXTERNAL EMAIL)

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Good morning all!

Is there a wireless temperature monitoring system that you would recommend?





Matthew Roark, HTL(ASCP)CM
Histology Specialist
Laboratory

P 573-331-3982 | M 573-979-1925 | F 573-331-5049 
mro...@sfmc.net

Saint Francis Healthcare System
211 Saint Francis Drive
Cape Girardeau, MO 63703

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Re: [Histonet] Cassette printers

[Cooper, Brian via Histonet]

Good Morning Paula, 

All I've ever used is the Leica IPC Cassette printer across my last few 
institutions.  They are absolute workhorses; printing is fast and beautiful and 
the maintenance is really easy.  The only downsides I can think of is that the 
IPC has a large footprint, and the ink is really pricey.  You're supposed to 
change the cartridge every 3.5 months or 60,000 prints or so.  We never hit 
that volume so we're always throwing away ink! 

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Paula Sicurello via Histonet  
Sent: Wednesday, December 28, 2022 10:44 AM
To: HistoNet 
Subject: [Histonet] Cassette printers (EXTERNAL EMAIL)

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Good Morning Listers,
We are in the market for a new cassette printer.  Has anyone used the General 
Data LaserTrack FLEX system?  I'm interested in how well it actually prints on 
3 sides of the cassettes.
Any input about any other vendors printers is helpful.  I have quotes from 
General Data, Sakura, and Leica.
Please provide your opinions and related experiences with each of the vendor's 
cassette printer.  We currently have a General Data CL12 that is dying a slow, 
painful death.
Thanks oodles for your help.

Paula Sicurello
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[Histonet] Cryogenic Vials?

[Cooper, Brian via Histonet]

Good afternoon Histonet,

What type of containers are you using for storage of frozen tissue samples 
collected in the gross room?  Our current cryogenic vials have been on 
backorder for more than a year now, and they keep pushing back our delivery 
date.

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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[Histonet] Epon Resin Embedding Question

[Cooper, Brian via Histonet]

Good afternoon Histonet,

Asking this question for a friend:  Is there a way to correct an improperly 
prepared Epon resin embedded block?   Following embedding, the block is soft 
and "tacky" to the touch.  This block is proving VERY difficult to section.  
Any assistance from those of you doing EM in your labs would be greatly 
appreciated.

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Molds- cold vs warm

[Cooper, Brian via Histonet]

Thanks for saying this Jay!! I have to say, it's been a while since we've had 
such a great response on Histonet!! Everything you said is spot on.

Happy Friday everyone!

Thanks,

Brian Cooper
Histology Supervisor
Children's Hospital Los Angeles
Sent from my mobile





On Sep 9, 2022 2:37 PM, Jay Lundgren via Histonet 
 wrote:
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Whoever is telling you to use cold molds needs to go back to clown college.

That is totally, 100%, absolutely, wrong.

There is some debate as to embed "wet" (cassettes submerged in paraffin
bath) or "dry", and I will accept either, as mostly a matter of personal
preference.  BUT, in both of these cases, the molds are hot.

I have been a Histotech for five decades, trained at Armed Forces Institute
of Pathology (back when that used to mean something) and I have NEVER seen
anyone using cold molds.

It is a guaranteed way to get cold fractures and cracks in your blocks, or
to pop tissue out when you are cutting, which might be irretrievable. Just
think how much time all those re-embedded blocks are going to save you!

Also, you won't be able to easily re-position specimens in the block, to
put them "on edge" or "on end", for example.  The tissue will instantly
stick to the cold mold.  And if you want to re-position it, guess what,
you'll have to warm the mold up to get the tissue unstuck.  How's that
(non-existent anyway) time savings now?


If you want to prove to whatever jackass suggested this that they are
wrong, get a big stack of every histopathology textbook you can find.
There is nothing in any of them talking about paraffin embedding with cold
molds.

As a matter of fact, every single textbook will specify molds at the same
temp as paraffin.

Anyway, it doesn't even make sense, thermodynamically.  Heat travels from
hot to cold.  Those "cold" molds will be the same temperature as the
paraffin, almost instantly. Did it take a tiny amount of heat out of the
hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The
amount of heat from the paraffin used to warm the mold is trivial compared
to the total heat of the system. That's why cold plates have huge, noisy
refrigeration units.  You can't argue with thermodynamics.

If you are having trouble getting your blocks to release, use mold
release!  Viola!  
https://secure-web.cisco.com/1vhENcmRngDgLubdLEYMzzWWUK4ILg_WIJNnMutz67Oikk5LSg5SqF6OvSQqMWpr4MIirbF_ExGbIXm9Usdm35LUk87pXYTIvPVdKY5u2dRCdo_Ss-iuZ4nCOa0nPTIpPec8zwvOBcVIE7eM7o-flt9BAIGK0ZOw4K3HOXwNiLmQBnD0hFb9pgrU0ZuPnk5llOYCeJ5b2Pmkp2B9UPlVvxPMI3-iHRILtOB4kPL45PII_yUJnJhFYAryeid5lrITtm-w0KNyKrfJVI0mHy47Niz0TEpxxvl3DoTDmq-umsyN3BucCj2B-aJFqJ-AW3thtXSEk-Nl0NzBBrSxw8cPzSrKsVww7cCLh_krbh7VXKlRiRGF41o3UKk_oEQuHGIEeYlUNLnpLndnkSH0cwR3nNWhq3Cy8hw6ws0Ka8kYRH8_TVttsOh_lQbO4tm6_i-fdNOZxcR_7t-QeE9aW5YP1hg/https%3A%2F%2Fwww.statlab.com
 I used to think it was
superfluous, but now I consider it compulsory.  This is probably the answer
to most of your issues.

I don't know who is suggesting using cold molds, but I can pretty much
guarantee that it's a pathologist who thinks his slides are taking too
long, and knows nothing about histopathology, or a lab manager, who knows
nothing about histopathology.  This next part is directly to them.

To Whoever Suggested Cold Molds:  The answer to getting your slides out
quicker is buying more equipment and hiring more techs, and holding
everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding).
Making nonsensical, uninformed suggestions only exposes your ignorance.

Please feel free to show them this reply.

Sincerely,

Jay A. Lundgren, M.S., HTL (ASCP)
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Re: [Histonet] Automated ihc staining of bone

[Cooper, Brian via Histonet]

Charles, 

Try and blot your sections dry before placing them into the oven.  After 
picking up your section, place your slide onto a flat surface and take a paper 
towel or piece of filter paper, and gently press it down on top of the section 
to wick away all of the moisture.  We use this technique frequently here on 
bone marrow cores.

Thanks, 

Brian D. Cooper, HT (ASCP)CM QIHC| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Charles Riley via Histonet  
Sent: Tuesday, August 30, 2022 12:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated ihc staining of bone (EXTERNAL EMAIL)

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I am working on trying to get IHC stains optimized on bone samples of rat 
tibias. The issue I am running into is that the periosteum is separating from 
the bone marrow and wrinkling up over top of the rest of the sample.

If anyone has any techniques to prevent this wrinkling/detachment of tissue 
from the main section during automated ihc staining it would be greatly 
appreciated.
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Re: [Histonet] Slide Printer

[Cooper, Brian via Histonet]

Similar story here, except we went with SlideMates.  Before AB LIS 
integration, we were able to have CoPath print a barcode on our cassettes 
(Leica IPC cassette printer). The SlideMate Field Service Reps were able to 
translate that barcode info on the slide printers (which were still standalone 
at that point).  That barcode on the cassettes translated to a patient name and 
accession number on each slide.  

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Terri Braud via Histonet  
Sent: Friday, August 5, 2022 12:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Slide Printer (EXTERNAL EMAIL)

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We've been using our Leica IPS and IPC printers for over 15 years, first as 
stand-alone units, and now, integrated into CoPath.  What a workhorse these 
instruments this have been!  Their modern equivalent is almost identical, so 
parts are readily available.  My recommendation would be to repair or replace 
with the same. 

Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-3874
  Honesty
AccouNtability
    AgiLity
    CoLlaboration
  CoMpassion

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Friday, August 5, 2022 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Histonet Digest, Vol 225, Issue 4

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Today's Topics:

   1. slide printers (Colleen Forster)


--

Message: 1
Date: Thu, 4 Aug 2022 17:15:11 -0500
From: Colleen Forster 
To: histonet-request 
Subject: [Histonet] slide printers
Message-ID:

Content-Type: text/plain; charset="UTF-8"

HEllo Histonetters,

We are a research lab that has been using the Leica IPC/IPS printed for many 
years. Today the flash motor went out in the IPS. We are now looking into 
updating the slide printer.

We are research only and NOT hooked into any LIS system. It would be a stand 
alone process. Can some of you share what you have and the pros/cons?
I need to start the search for the replacement I would like.

Thank you in advance.

--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930


--

Subject: Digest Footer

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End of Histonet Digest, Vol 225, Issue 4



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Re: [Histonet] HSV testing

[Cooper, Brian via Histonet]

We run Cell Marque's HSV1 and HSV2 on our Leica Bond platform here.  

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Nancy Schmitt via Histonet  
Sent: Wednesday, July 6, 2022 4:38 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HSV testing (EXTERNAL EMAIL)

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Hello-
What are you using for HSV IHC testing?
Thank you
Nancy

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Re: [Histonet] microtome pm

[Cooper, Brian via Histonet]

Once a year is good for us.  Just an added thought: we always make sure they 
change the extension springs in the cassette clamp with each PM as well.  

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Paula via Histonet  
Sent: Tuesday, June 28, 2022 1:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microtome pm (EXTERNAL EMAIL)

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Hi everyone,

 

How often do you PM your microtomes?  Currently, we have them done every 6 
months but I'm thinking once a year is sufficient and I wanted to get some 
input before we decide.

 

Thank you in advance,

Paula

Bio-Path Medical Group

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Re: [Histonet] Melanoma Control Blocks?

[Cooper, Brian via Histonet]

Good morning Histonet!  

Just wanted to let you know that we've received a lot of offers for help on 
melanoma controls since my email yesterday.  Thank you to all who have 
responded. I think we will likely have melanoma controls for many years to come 
if these all pan out!  Much appreciated Histonet; when one of us needs help, 
this is always such a valuable resource.   Happy almost Friday!  

Thanks, 

Brian

-Original Message-
From: Cooper, Brian via Histonet  
Sent: Wednesday, March 30, 2022 10:57 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Melanoma Control Blocks? (EXTERNAL EMAIL)

CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside 
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Good morning Histonet!

Does any of you have any melanoma control blocks you'd be willing to share?  We 
have a bunch of other controls we can trade for: CMV, EBV, Pneumocystis, HSV 1 
or HSV2 just to name a few.  Please message me offline if you're able to help!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>

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[Histonet] Melanoma Control Blocks?

[Cooper, Brian via Histonet]

Good morning Histonet!

Does any of you have any melanoma control blocks you'd be willing to share?  We 
have a bunch of other controls we can trade for: CMV, EBV, Pneumocystis, HSV 1 
or HSV2 just to name a few.  Please message me offline if you're able to help!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] best paraffin

[Cooper, Brian via Histonet]

Paraplast for processing and Paraplast Xtra for microtomy at our institution.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Jay Lundgren via Histonet  
Sent: Thursday, March 10, 2022 9:22 AM
To: John Garratt ; Rinker, Jeffrey 
; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] best paraffin (EXTERNAL EMAIL)

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Paraplast plus is my personal favorite.

On March 10, 2022, at 11:04 AM, John Garratt via Histonet 
 wrote:

The paraffins available from lab suppliers (not candle makers) is all of 
excellent quality these days. There is some variability, but choice of paraffin 
usually comes down to personal preference.
If you are having problems cutting it may well be related to other factors like 
fixation and processing, and of course, the microtomy.
What are the issues you are encountering?

John
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On Thu, Mar 10, 2022 at 7:21 AM, Rinker,Jeffrey via Histonet 
 wrote:

> I was wondering what people think is the best paraffin to use. I find it hard 
> to get a good read on what is best for our lab. We do around 60-100 blocks a 
> day and do a bx and surgical run overnight. I think that we are having 
> problems with cutting because we are not optimized and i am trying to fix 
> that.
> --
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Re: [Histonet] contents of Histonet digest

[Cooper, Brian via Histonet]

Our formalin vendor is sending us certificates of analysis with every shipment 
now. We review these forms to ensure the lot is acceptable and file them in a 
binder.

Thanks,

Brian Cooper
Histology Supervisor
Children's Hospital Los Angeles
Sent from my mobile

On Mar 4, 2022 7:18 AM, "Maimone-Schoen, Michele via Histonet" 
 wrote:
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respond unless you expected this message and recognize the email address: 
histonet-boun...@lists.utsouthwestern.edu.

How are labs addressing the new CAP standard ANP.10041: Quality of Formalin 
Monitoring?   Thank you.


Michele Maimone-Schoen, MS
Manager, Anatomic Pathology
New York-Presbyterian Hospital
525 East 68th Street, Starr 1003
New York, NY  10065
212-746-2633 (Office)
212-746-5007 (Fax)
646-856-1738 (Cell)

Be Amazing!

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[Histonet] Stat6 control material

[Cooper, Brian via Histonet]

Good afternoon Histonet,

Do any of you have any STAT6 control material available to share? Please 
message me offline if you're able to help; we have lots of control materials 
that we can trade.

Thanks,

Brian Cooper
Histology Supervisor, Children's Hospital Los Angeles
bcoo...@chla.usc.edu
Sent from my mobile phone
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[Histonet] Histochemical autostainer

[Cooper, Brian via Histonet]

Dako (Agilent) Artisan is the way to go.

Thanks,

Brian Cooper
Histology Supervisor
Children's Hospital Los Angeles

Sent from my cell phone, so please excuse any funny typos.

On Jan 26, 2020 5:47 PM, "Azam, Muhammad via Histonet" 
 wrote:
Hi all:

Our special stainer went kaput last week. Anybody would recommend a reliable 
benchtop autostainer for histochemical stains.

V/r

Muhammad Azam, MD
Chief, Pathology & Laboratory Medicine Service (113)
James H. Quillen VAMC
Mountain Home, TN
Phone: 423-979-3529
Cell: 423-741-9872

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[Histonet] Deep Water Bath with Lighting

[Cooper, Brian via Histonet]

I really like the TFB 35 flotation baths from Medite. They make a shallow and a 
deeper dish version; you'll want to go with the deeper dish. I'm not in front 
of one right now, but I suspect they're about 3" give or take.

Thanks,

Brian Cooper
Histology Supervisor
Children's Hospital Los Angeles

Sent from my cell phone, so please excuse typos


On Dec 18, 2019 3:45 PM, Sandra Cheasty via Histonet 
 wrote:
Ho-ho-ho all,
I'm looking for a deep water bath, (3" minimum), that has an 
interior light source. Most of them seem to be only 2" tall, or have a slanted 
front control panel that none of us in the lab want. Does anyone know of a 
brand of flotation water baths that are 3" deep and have interior lighting?
Cheers!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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[Histonet] Cryostat Samples for Molecular Testing

[Cooper, Brian via Histonet]

Good afternoon Histonet!

We would like to know what decontamination procedures you are performing to 
prevent cross-contamination in between cutting scrolls for frozen samples for 
molecular testing.  We've had a few complaints of trace carryover from one 
sample to the next.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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[Histonet] TJP2 antibody

[Cooper, Brian via Histonet]

Good morning Histonet,

Is anyone out there working with TJP2 (tight junction protein) antibody?  If 
so, we'd love to hear from you, and ideally, get a slide or two that 
demonstrates loss of this protein in human liver.  We're willing to provide you 
with a control block for something else if you can help us out.  Pediatric 
institutions-this one may be in your wheelhouse!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] Sectioning over processed GI biopsy

[Cooper, Brian via Histonet]

Did you try soaking them face-down on your flotation bath for 20 seconds or so, 
prior to placing on a very wet ice bath (over a Labsorb or paper towel)?  It 
sounds like these GI's were processed with an inappropriate protocol on your 
tissue processor.

Good luck.

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcoo...@chla.usc.edu 

-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 18, 2019 9:49 AM
To: Histo List
Subject: [Histonet] Sectioning over processed GI biopsy (EXTERNAL EMAIL)

What is the best way to get a section from an over-processed GI biopsy?
Paraffin keeps pulling away from the tissue and nothing sections. Tissue
very hard even after lenghty time on ice water bath

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] Mini Lab Incubators?

[Cooper, Brian via Histonet]

Good Afternoon Histonetters!

Does anyone out there have any recommendations as far as Mini Lab Incubators 
go?  Have looked at the VWR Digital Mini Incubator and LabNet International 
Mini Incubator online this morning.  Haven't seen much in the way of comments 
on these products; would love to hear your feedback.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu

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Re: [Histonet] New Lab

[Cooper, Brian via Histonet]

Know what else you can't have enough of?  Data ports.  We just went live with 
barcoding and tracking and we had to add TONS of them in nearly every room!

Good luck!

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 


-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, January 11, 2019 10:28 AM
To: Campbell, Tasha M.
Cc: Histonet
Subject: Re: [Histonet] New Lab (EXTERNAL EMAIL)

Hi Tasha,

How fun!  All I can say is ventilation, ventilation, ventilation.  If you
can't get proper ventilation I recommend Sentry Air Systems.  They have
portable fume extractors that use a series of carbon filters, to suck up
the xylene or formaldehyde vapors.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



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please contact the sender and delete the material from any computer.


On Fri, Jan 11, 2019 at 5:37 AM Campbell, Tasha M. via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello, I am a small GI lab and our facility is moving to a brand new
> building!  The building has yet to be built so I have full control on how
> to design my lab.  I will have about 1000-1500 sq ft.  I was just wondering
> if anyone had any tips for me?  One thing I want to change is the type of
> flooring.  Right now we have the normal 12 x 12 in tiles that are built in
> most buildings.  Is there any other type of flooring that is better
> especially when dealing with the paraffin? And the fact that I am scraping
> the floors?   I use to work at the local hospital here and they remodeled
> the lab there and they put vinyl flooring in that is supposed to be made
> for labs.  Come to find out during the first xylene spill that xylene melts
> vinyl!  So I know I don't want to go that route.
>
>
> Thanks!!
>
>
>
>
> Tasha Campbell, B.S.,HTL(ASCP)
> Frederick Gastroenterology Associates
> 310 W. 9th St.
> Frederick, MD 21701
> 301-695-6800 ext. 144
>
>
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Re: [Histonet] Brachyury

[Cooper, Brian via Histonet]

We use the mouse monoclonal (1H9A2) from AbCam.  Works like a charm.

https://www.abcam.com/brachyury--bry-antibody-1h9a2-ab140661.html 

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Oler, April via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, December 26, 2018 6:20 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Brachyury (EXTERNAL EMAIL)

Good morning Histonet,

Has anyone had luck finding a Brachyury antibody that works well? After the 
Santa Cruz (H-210) was discontinued, I've been unable to find an antibody to 
replace the H-210. I've tried two antibodies (Santa Cruz D-10 and BioSB X1A02), 
but I was unable to validate either of them due to inconsistent staining 
results.

Thank you in advance for any information you can provide.

April Oler, HT
Lead Tech IHC/DIF/Muscle
Michigan Medicine
University of Michigan
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Re: [Histonet] Low Oxygen Sensor/Alarms

[Cooper, Brian via Histonet]

Hi,

Posting this question again to my colleagues on Histonet, as I have yet to 
receive a response.   In regards to newly revised LABGEN checklist,  GEN.77550 
states "In areas where liquid nitrogen is used, there are oxygen sensors with a 
low oxygen alarm mounted in an appropriate location and sufficient airflow to 
prevent asphyxiation."   For those of you who already do this, can you please 
tell me the type and model of sensor you're using?

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
bcoo...@chla.usc.edu


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[Histonet] Low Oxygen Sensor/Alarms

[Cooper, Brian via Histonet]

Hi,

Looking at the newly revised LABGEN checklist this afternoon.  GEN.77550 states 
"In areas where liquid nitrogen is used, there are oxygen sensors with a low 
oxygen alarm mounted in an appropriate location and sufficient airflow to 
prevent asphyxiation."   For those of you who already do this, can you please 
tell me the type and model of sensor you're using?

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
bcoo...@chla.usc.edu


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Re: [Histonet] Experience (especially long term)

[Cooper, Brian via Histonet]

I've used both.  Agree that the Sakura's are awesome; used them for years at 
another institution.  We currently have two of the Histostars, and we actually 
like the design a little better.  The Histostar's hot plates are set back a 
little further than those on the Sakura model.  In my case, this results in 
much fewer instances of "paraffin sleeves" on my lab coat!  The remaining 
plastic space in front of the hot plate is a great place to align GI biopsies 
in your mold during orientation, or to flatten out and scrape paper wrapped 
cell block material.   On our Histostar models (probably about 5 years old now) 
we don't have the ability to adjust the temperature of the cold plate.  I do 
miss that, because we've found ours too be a little too cold, and we sometimes 
get cracks in our paraffin blocks (this is REALLY inconvenient on needle 
biopsies). I believe that on newer models, this feature has been added.   

That's my two cents worth...

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Kristyn Ferber via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 27, 2018 10:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Experience (especially long term) (EXTERNAL EMAIL)

Hello Histonetters!

I'm looking to gather histotech's experience with Thermo Scientific's
HistoStar embedding workstation. I am very familiar with Sakura and think
it's top notch as far as service and support and, especially, longevity.
How does everyone feel the HistoStar compares with the Tissue Tek system if
you've used both?

Thanks in advance!

Kristyn Ferber, HTL
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Re: [Histonet] Luxol fast Blue Question

[Cooper, Brian via Histonet]

Hi Lisa, 

We keep them in solution at 56-60 degrees overnight, and typically start 
differentiation between the 15-20 hour range. 

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 


-Original Message-
From: Chapman, Lisa via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, September 12, 2018 12:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Luxol fast Blue Question (EXTERNAL EMAIL)

Hello All,

When performing a LFB for myelin and nissl, how long does everyone keep their 
slides in the LFB solution for and what temp do you use?  Most procedures say 
"overnight" and Sheehan states 16-24 hours.

Any advice would be greatly appreciated!  Thank you in advance!

Lisa Chapman HT (ASCP)
ACL Laboratories
Milwaukee, WI
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Re: [Histonet] tissue processor

[Cooper, Brian via Histonet]

We absolutely love our Leica Peloris II.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Lisa Brenner via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, February 02, 2018 10:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] tissue processor (EXTERNAL EMAIL)

Hello,

   We are in the market for a new vacuum infiltrating tissue processor. Our old 
VIP has been a work horse but needs replacing. What is everyone using? What 
works well? What's new?

Lisa Brenner


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[Histonet] Type of Nitrile Gloves in your lab

[Cooper, Brian via Histonet]

Hi,

Does anyone out there use a brand of nitrile glove that they really like?  The 
ones we use here are not so good.  When we pull them on, the part closet to the 
wrist often tears off, leaving behind a nice blue rubber band.  At least 
several gloves per box have tears in them before we even put them on.  One of 
our Quality Managers asked us to come up with alternatives.  Can you help me 
out?   You can email me offline if you don't want to name particular brands for 
everyone to see.  PLEASE HELP!!!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu


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Re: [Histonet] LFB-H controls

[Cooper, Brian via Histonet]

We use spinal cord.

-Original Message-
From: Diane Satterfield via Histonet [histonet@lists.utsouthwestern.edu]
Received: Friday, 03 Nov 2017, 7:39AM
To: 'histonet@lists.utsouthwestern.edu' [histonet@lists.utsouthwestern.edu]
Subject: [Histonet] LFB-H controls (EXTERNAL EMAIL)

I am getting ready to learn how to do the LFB-H staining on brain tissue.  I 
was wondering want I would use for a control with this staining.

Diane
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Re: [Histonet] Lab assistants

[Cooper, Brian via Histonet]

Too funny Jay!  I take it you've met my old boss!!  LOL! 

Brian

-Original Message-
From: Jay Lundgren via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, October 12, 2017 1:04 PM
To: Hannen, Valerie
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Lab assistants (EXTERNAL EMAIL)

The most important role of the lab assistant in the Histology laboratory is to 
be the target of middle management ire.

  Sincerely,

   Jay A. Lundgren, M.S., HTL(ASCP)

On Wed, Oct 11, 2017 at 4:00 AM, Hannen, Valerie via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Good Morning,   We are having a debate about what a Lab assistant can and
> can not do in the State of Florida.  So, those of you in Florida 
> please weigh in.  Can they make alcohol  dilutions and use those alcohols to:
>
> 1)  Change an automatic stainer
>
> 2)  Change the tissue processor
>
>
> What about recycling said alcohols?   Are they allowed to pour formalin
> into the processor wells? Can they fill small bottles with formalin to 
> be used by doctor's offices?  The list could go on and on... but I 
> will stop here.
>
> Thanks so much for any insight into this!
>
>
> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
> Section Chief, Histology
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville,Florida 32796
> T: (321)268-6333 ext. 7506
> F: (321) 268-6149
> valerie.han...@parrishmed.com
> www.parrishmed.com
>
> ==
> "This email is intended solely for the use of the individual to whom 
> it is addressed and may contain information that is privileged, 
> confidential or otherwise exempt from disclosure under applicable law. 
> If the reader of this email is not the intended recipient or the 
> employee or agent responsible for delivering the message to the 
> intended recipient, you are hereby notified that any dissemination, 
> distribution, or copying of this communication is strictly prohibited. 
> If you have received this communication in error, please immediately 
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Re: [Histonet] What is the best microtome?

[Cooper, Brian via Histonet]

Microm HM325 gets my vote.

-Original Message-
From: Dawn Bugge via Histonet [histonet@lists.utsouthwestern.edu]
Received: Wednesday, 13 Sep 2017, 3:04PM
To: histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu]
Subject: [Histonet] What is the best microtome? (EXTERNAL EMAIL)

Hello everyone,

What is your favorite microtome?  I am helping a lab with purchases and I
only have experience with the Finesse ME+ and an old Leica.  Any thoughts
would be great.

Thanks.

--
Dawn R Bugge
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[Histonet] Need help identifying a product!!!

[Cooper, Brian via Histonet]

Happy Friday Histonet!

Our cassette printed died this week, and we've been handwriting cassettes for 
the last couple of days.  It's been YEARS since I had to do this; BOY has my 
handwriting atrophied since then!  I remember we used to have this little metal 
rack that held the cassettes perfectly stationary when you wrote on them; the 
rack held about 20-25 cassettes in 4 or 5 rows.  Do any of you remember these?  
I've been searching online for a couple days, but Google and all the lab supply 
sites just aren't catching what I'm looking for.  Our printer will be fixed in 
a few hours, but just in case, I'd like to have one of these racks for the 
future.  Any ideas?  Should I check antique shops?  :)

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu


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Re: [Histonet] Leica SelecTech Stains

[Cooper, Brian via Histonet]

Hi Amanda, 

We used to use their Define Solution, but now we simply stain with their 
Hematoxylin 560 MX and Alcoholic Eosin-Y.  1% acid alcohol (5 quick dips) for 
differentiation and dilute ammonia water for bluing.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 

-Original Message-
From: Amanda Reichard via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, July 10, 2017 6:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica SelecTech Stains (EXTERNAL EMAIL)

For those of you using the SelecTech stains...

Do you use the entire system (define and blue buffer), or just the hematoxylin 
and eosin?

It seems from previous posts everyone who has used it is very happy with it, 
I'm just curious if we can stick with a less pricey differentiation and bluing 
solution.

Thanks in advance

Amanda Reichard, HTL (ASCP)cm
Histology/Cytology Supervisor
Licking Memorial Health Systems
1320 W. Main St.
Newark, OH 43055
(220) 564-4163
areich...@lmhealth.org




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[Histonet] Empty Chemical Containers

[Cooper, Brian via Histonet]

Happy Lab Week Histonet!

How are you all disposing of empty chemical containers?   Triple rinsing and 
throwing them in regular trash (with the labels defaced,) or having the empty 
containers disposed of by your outside chemical contractors? I'm sure this 
answer will vary from state to state, and institution to institution (and 
chemical to chemical for that matter).  Sorry if this has been asked 
previously-I searched the archives, and an answer wasn't readily found.  This 
came up during safety rounds the other day, and our institution's policy on 
this is pretty vague.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu


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Re: [Histonet] BRAF V600E

[Cooper, Brian via Histonet]

Thank you to all who replied to my inquiry about BRAF on the Bond Max.  As 
always, I'm truly grateful for the valuable resource that is Histonet. 

Happy Holidays everyone!

Brian

-Original Message-
From: Cooper, Brian via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, December 13, 2016 2:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] BRAF V600E

Good Afternoon Histonet!

It's been a few years since I posted this question initially; thought I'd give 
it a try again.  Is anyone successfully utilizing Roche's BRAF V600e (VE1) (or 
Spring Bioscience's) antibody on a Leica Bond Max?  If you are, I'd really 
appreciate hearing how you did it.  This antibody is quite expensive, and we 
don't particularly want to burn through massive amounts of money trying to 
reinvent the wheel.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>



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[Histonet] BRAF V600E

[Cooper, Brian via Histonet]

Good Afternoon Histonet!

It's been a few years since I posted this question initially; thought I'd give 
it a try again.  Is anyone successfully utilizing Roche's BRAF V600e (VE1) (or 
Spring Bioscience's) antibody on a Leica Bond Max?  If you are, I'd really 
appreciate hearing how you did it.  This antibody is quite expensive, and we 
don't particularly want to burn through massive amounts of money trying to 
reinvent the wheel.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu



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Re: [Histonet] On the hunt for new microtomes!

[Cooper, Brian via Histonet]

We've used Microm microtomes for years and they have always been 100% trouble 
free.

-Original Message-
From: Jennifer MacDonald via Histonet [histonet@lists.utsouthwestern.edu]
Received: Monday, 01 Aug 2016, 12:45PM
To: Rene J Buesa [rjbu...@yahoo.com]
CC: Mary Faith Encarnacion [mfb.encarnac...@gmail.com]; 
histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu]
Subject: Re: [Histonet] On the hunt for new microtomes!

Lecia makes the Sakura microtome.  We have both Sakura and Microm
microtomes in our student lab.  They have both proved to be reliable.
There is a difference between a Shandon microtome and a Microm microtome.



From:   Rene J Buesa via Histonet 
To: Mary Faith Encarnacion ,
"histonet@lists.utsouthwestern.edu" 
Date:   08/01/2016 12:42 PM
Subject:Re: [Histonet] On the hunt for new microtomes!



I don't know now, but some years ago Thermo instruments were less that
reliable. Try Leica or even better Sakura.René

On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet
 wrote:


 Hey HistoNet,

Thanks to everyone who helped me out by providing their opinions on
embedding centers.  This time, I need everyone's thoughts on microtomes.
My lab is debating between the Thermo/Microm HM355S and the Leica RM2255.
Your thoughts and advice are very much appreciated!  If there are any more
I should try, let me know!

Thanks again,
Mary Faith
Histology Supervisor
VA Palo Alto Health Care System
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Re: [Histonet] block scrapers

[Cooper, Brian via Histonet]

I bought everyone really old pocket knives from flea markets--basically the 
same thing as what Jay said.  For the most part, these old knives were already 
really dull and didn't require any assistance to dull them further.

Good luck.

Brian


-Original Message-
From: Jay Lundgren via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, July 21, 2016 12:04 PM
To: Lauren Sweeney
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] block scrapers

Buy cheap paring knives at the dollar store and blunt the edges?

On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Hi all,
>
> My lab is in need of some tools to scrap the paraffin off the edges of 
> the blocks after embedding. Does anyone have any recommendations for me?
>
>
> Thanks!!
> L
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Re: [Histonet] Leica ASP6025 Tissue Processor and Cryostats

[Cooper, Brian via Histonet]

So we actually have a Leica CM1950 in our Histo Lab, as well as a Fisher NX70 
Cryostar in our Frozen Section Room in the OR.  They are both excellent 
cryostats (I actually learned how to cryosection on the Leica).  When we were 
in the market for a new Cryostat a year ago, we went with the NX70 because of 
the ergonomic flexibility and cold disinfection--both really cool (ok, pun 
truly wasn't intended there) features!  Check em both out, and see all the cool 
bells and whistles.

Good luck!

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Fortin, Joyce via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, March 10, 2016 10:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica ASP6025 Tissue Processor and Cryostats

Could anyone who is using the Leica ASP6025 Tissue Processor please send me 
their opinions of it-pros and cons?
Also, which of the Leica cryostats you like the most?  We want the vacuum and 
UVC disinfection models.  Pros and cons...
Thank you!



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Re: [Histonet] Microtomes

[Cooper, Brian via Histonet]

This has been a Histonet post in the past; techs are fiercely loyal to their 
favorite brand and I'm sure you'll see people disagree with me as soon as I hit 
send!

I prefer Microm.  In our experience, block alignment is easier with the 
Microm's because the X and Y axis orientation knobs are positioned in such a 
manner that you can adjust block orientation with your left hand while your 
right hand is on the flywheel.  This allows you to keep a constant sight line 
of the entire depth of the block face in relation to the knife.  With the Leica 
microtomes, the positions of these screws are moved to the right, and the 
orientation of the Y axis requires you to either use two hands at once, or for 
you to move your left arm across your sight line to orient the Y axis.  While 
certainly not insurmountable; this just makes block orientation take a little 
longer.  Since we frequently have to recut blocks from different institutions, 
this is a big deal here.  I'm sure the peeps from the other camp are going to 
say that it's all in the technique.  To that I say, YEAH, YEAH, YEAH  :)

Both of these machines are well designed and will provide years and years of 
excellent service as long as they are well maintained.  The best thing you can 
do is ask to demo both machines and see what you like best. 

Good luck!

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 


-Original Message-
From: HERRINGTON, SHEILA via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, January 27, 2016 11:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtomes

We are looking to purchase two new microtomes and were wondering on thoughts 
and recommendations from the experience of the people that actually use them.  
Pros and cons would be extremely valuable on ergonomics, reliability and 
overall performance.

Sheila Herrington, RT
Technical Lead, Immunohistochemistry and Histopathology Kelowna General Hospital
2268 Pandosy Street,
Kelowna, B.C. V1Y 1T2

250-862-4300 x 7510

sheila.herring...@interiorhealth.ca



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Re: [Histonet] Alliance Rubber Bands

[Cooper, Brian via Histonet]

Awesome post Brent!   I thought I was the only one who had a strong opinion 
about rubber bands! I never used the orange color bands you mentioned, but the 
Alliance Pale Crepe Gold #64 were pretty awesome too.  Agree totally--cheap 
rubber bands HURT

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: Brent Adams via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, January 27, 2016 11:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Alliance Rubber Bands

Hey Histonetters,



I don't endorse products but this one is really good.



I don't get a kick back and no relatives work for the company, I just wanted

to give you all a good product to use if you are looking. I looked up the 
company

and found they are the last U.S. Made Rubber Band company and based out of

Arkansas.



I think I have had 2 eye injuries and multiple painful mishaps from

rubber bands that break far to easily so from that perspective I feel

I can give this product a thumbs up.



For the last 4 years I have been using  Alliance Rubber Company

non- latex rubber bands. The size 64 (3.5 x 1/4) work best on Slide folders

I have found. The Orange color rubber bands are easy to see and

hold the file folders together great and they rarely break.



I know it sound silly to talk about rubber bands this way but I was

really getting tired of being snapped by faulty rubber bands when trying to

wrap slide folders or paperwork.



I just had my first rubber band by Alliance snap a couple months ago which 
caught me

off guard cause it had been so long since that happened. It was then I look to

see what brand we were using and it turned out that we had been using the same

NON-latex rubber bands from the Alliance Rubber Company since I opened the 
Laboratory in 2012.

Before that at other labs breaking rubber bands were just part of the daily Job.



I think purchasing has gotten their best price on Amazon but they also use 
staples and

Office depot for supplies. I informed them that I only want these rubber bands 
and they

can get their best pricing where ever.



My fingers hurt a lot less after distributing slide folders and I don't have to 
swear as much. Ha Ha.

Funny how little things can really bother us.



FYI



Brent Adams - BS, LPN, HT


www.acadianagastro.com

Acadiana Gastroenterology Associates, LLC
439 Heymann Blvd
Lafayette, LA  70503

tel:  (337) 269-1126
fax:  (337) 269-1476
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Re: [Histonet] Best Glass Coverslipper?

[Cooper, Brian via Histonet]

Thank you to all who responded to my inquiry about the best glass coverslipper! 
 Everyone loves their Leica CV5030's and Sakura Glas G2's!

Thanks, 

Brian  


-Original Message-
From: Cooper, Brian via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, January 21, 2016 2:36 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Best Glass Coverslipper?

Hey Histonet!

What's the best automatic glass coverslipper out there?  I only have experience 
with the Sakura tape slipper, which is AWESOME, but alas, we can't get one 
here.  Space is definitely an issue; would love to hear your experiences!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu<mailto:bcoo...@chla.usc.edu>



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[Histonet] Best Glass Coverslipper?

[Cooper, Brian via Histonet]

Hey Histonet!

What's the best automatic glass coverslipper out there?  I only have experience 
with the Sakura tape slipper, which is AWESOME, but alas, we can't get one 
here.  Space is definitely an issue; would love to hear your experiences!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu



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[Histonet] Document Control Systems

[Cooper, Brian via Histonet]

Happy Friday Histonetters!

For any of you out there using Title 21, Medialab or iPassport for document 
control (or anything else for that matter),  I would love to hear your feedback 
on what it's like to use the systems.  Pros/cons/responsiveness of the 
companies to your concerns-anything at all!  Your feedback would be greatly 
appreciated!  You can email me offline if you prefer.  We've seen demos from 
all three vendors, but would absolutely love to hear from people who actually 
use these systems.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu



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[Histonet] PFIC Case Material

[Cooper, Brian via Histonet]

Good evening Histonet,
Would any of you have access to, and be willing to share either blocks, or 
unstained slides from patients known to have progressive familial intrahepatic 
cholestasis (PFIC2 or PFIC3)?  We're interested in working up a few antibodies, 
yet a sufficient number of positive cases is proving to be elusive.  I'm 
willing to trade for control blocks that may have in our library.  Please 
contact me if you can help.
Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu



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[Histonet] Spirochete Control Block?

[Cooper, Brian via Histonet]

Good afternoon Histonetters!

I'm fairly certain my chances of seeing a puppy dog, riding on the back of a 
unicorn, sitting under a blue moon are greater, but does anyone have a block of 
spirochetes they'd be willing to share? Recently, we've gotten some great 
controls from these posts and thought we'd give this a shot!  We're more than 
willing to trade!  Please contact me directly if you don't want to post your 
responses.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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[Histonet] CMV antibody clone

[Cooper, Brian]

Good afternoon Histonet,

Just taking a quick poll to find out which CMV clone most folks are running in 
their labs.  Your feedback is much appreciated.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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Re: [Histonet] CMV antibody clone

[Cooper, Brian via Histonet]

Thank you to everyone who replied to this post.  In case you're wondering, I 
got like 8 responses today, and everyone who responded uses CMV (DDG9/CCH2) 
from either Cell Marque or Dako.

You guys are the best!

Thanks,

Brian

-Original Message-
From: Cooper, Brian [mailto:bcoo...@chla.usc.edu] 
Sent: Monday, July 13, 2015 12:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CMV antibody clone

Good afternoon Histonet,

Just taking a quick poll to find out which CMV clone most folks are running in 
their labs.  Your feedback is much appreciated.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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Re: [Histonet] Sakura SmartSection

[Cooper, Brian]

I went to Sakura to see that thing on a demo a few years back, and it was . . . 
scary!  The sales reps couldn't get it calibrated right, and it ripped tissue 
out of the blocks while it was trimming on a few occasions. Not one of us in 
the room (pretty much all experienced bench histotechs) could collect ribbons, 
though I suspect this could have been a learning curve type thing.  Aside from 
that, I will say that they had the ability to realign blocks pretty well 
perfected, and I was impressed by that.  

I went back to Sakura about a year later for something else, and our rep was 
still shaking her head about how bad that demo experience went!   Sounds like 
they may have worked out the kinks eh?

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357    
bcoo...@chla.usc.edu 


-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] 
Sent: Friday, July 10, 2015 12:26 PM
To: Histonet
Subject: [Histonet] Sakura SmartSection

Has anyone done any work with the Sakura SmartSection robot? We've had some 
blocks cut on it and have had good initial results. This could be a 
game-changer for histology staffing.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

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Re: [Histonet] Sakura SmartSection

[Cooper, Brian]

Ah!  I stand corrected.  I have to admit, I'm really leery about letting 
automated microtomes be in the same vicinity of renal or liver needle 
biopsies--are you guys comfortable with this?   
 
Brian


-Original Message-
From: Willis, Donna G. [mailto:donna.wil...@baylorhealth.edu] 
Sent: Friday, July 10, 2015 1:47 PM
To: Cooper, Brian; Morken, Timothy; histonet@lists.utsouthwestern.edu
Subject: RE: Sakura SmartSection

Brian,
I think the unit that Tim is talking about is something different.  His unit 
has not need for a tech to pick up sections.  The sections are placed on the 
slide by the robot.  

We are currently demoing the new Sakura Tissue Tek Auto Section Microtome that 
does not use a robot.  I will have to say that if it is the same unit that you 
looked at years ago, they have worked out the bugs.  It is a really nice unit.  
I technicians do not want the unit to leave when the demo is complete.

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager

Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com



-Original Message-
From: Cooper, Brian [mailto:bcoo...@chla.usc.edu] 
Sent: Friday, July 10, 2015 3:00 PM
To: Morken, Timothy; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Sakura SmartSection

I went to Sakura to see that thing on a demo a few years back, and it was . . . 
scary!  The sales reps couldn't get it calibrated right, and it ripped tissue 
out of the blocks while it was trimming on a few occasions. Not one of us in 
the room (pretty much all experienced bench histotechs) could collect ribbons, 
though I suspect this could have been a learning curve type thing.  Aside from 
that, I will say that they had the ability to realign blocks pretty well 
perfected, and I was impressed by that.  

I went back to Sakura about a year later for something else, and our rep was 
still shaking her head about how bad that demo experience went!   Sounds like 
they may have worked out the kinks eh?

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu 


-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
Sent: Friday, July 10, 2015 12:26 PM
To: Histonet
Subject: [Histonet] Sakura SmartSection

Has anyone done any work with the Sakura SmartSection robot? We've had some 
blocks cut on it and have had good initial results. This could be a 
game-changer for histology staffing.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

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Re: [Histonet] Adenovirus controls

[Cooper, Brian]

Good afternoon Histonet!  

I just wanted to say thank you to all who helped in our quest for Adenovirus 
control material.  Your generosity is truly appreciated, and once again, 
Histonet has proven to be an amazing resource!

Sincerely, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357  
bcoo...@chla.usc.edu 

-Original Message-
From: Cooper, Brian [mailto:bcoo...@chla.usc.edu] 
Sent: Thursday, May 28, 2015 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Adenovirus controls

Good morning Histonet, 

It's been about a month since I last sent out an inquiry about adenovirus 
control material, and I struck out badly with only one response!   Does anyone 
have a spare adenovirus block they'd be willing to trade for something else 
they might need?  We have a lot of control material available for special 
stains--Fungus, Gram +/-, AFB, etc . . .  For IHC, we have Eber and TONS and 
TONS of Tonsil!   You can contact me offline if you're more comfortable doing 
so.  Any help would be greatly appreciated.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edu 




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Re: [Histonet] : Paraffin block disposal

[Cooper, Brian]

At this institution, we cite CAP's guidelines (ANP.12500) in our retention 
policy as a minimum of 10 year retention, prior to disposal of patient tissues. 
 In practice we go further than that--we've never discarded a patient's blocks. 
 The only thing we ever gotten rid of was animal research tissues, and even 
then, sparingly.  They make great practice tissue blocks for histology 
students! 

Brian Cooper
CHLA

-Original Message-
From: Mayer,Toysha N [mailto:tnma...@mdanderson.org] 
Sent: Monday, June 08, 2015 7:02 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] : Paraffin block disposal

The regulation says two years.  I was always led to believe that for Pedi, it 
should 10 years past the age of 18.  Some facilities add the phrase 'past sign 
out'  onto the policy for disposal.  The methods can vary according to facility 
and state.  In some places that could mean in the trash, in others biohazard 
waste.  If confused check with another long standing facility  and a newer one 
in the area to get an idea of what should be done.  I have usually placed them 
in the biohazard trash, so that there would be no issues with anything.  

Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator 
Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer 
Center
713.563-3481

Message: 1
Date: Sat, 6 Jun 2015 10:24:42 -0700
From: Aimee Tolentino a.tolentin...@gmail.com
To: Arbaugh, Roberta rarba...@csdermatology.com
Cc: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin block disposal
Message-ID: 1f36aba5-452b-4556-8d1e-e5d09fdb2...@gmail.com
Content-Type: text/plain;   charset=us-ascii

That's a good question. I'd like to know the answer myself to that. :)

Sent from my iPhone

 On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta rarba...@csdermatology.com 
 wrote:
 
 Per CLIA we only need to keep paraffin blocks two years. What is the proper 
 way to dispose of them?
 
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 action or omission taken by you in reliance on it, is prohibited and may be 
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Re: [Histonet] Paraffin block disposal

[Cooper, Brian]

Hey Aimee,

This has been discussed several times on Histonet. It sounds like it depends on 
the institution. Since they're FFPE, pathogens are not a concern. I didn't 
reply to all because someone will shout out, What about CJD? and then I would 
have to punch them. They should be able to go into the regular trash though, 
since there is nothing that anyone can catch from them. Here, just like 
Genzyme, we are told to dispose of them as regulated, biohazard waste. You 
would have PHI concerns if the patient's name is on them, so they'll need to be 
identified first . . .

Thanks,

Brian Cooper, HT (ASCP)
Supervisor, Histology
Children's Hospital, Los Angeles

Sent from my Galaxy S5, so please forgive any weird typos . . .

-Original Message-
From: Aimee Tolentino [a.tolentin...@gmail.com]
Received: Saturday, 06 Jun 2015, 10:25AM
To: Arbaugh, Roberta [rarba...@csdermatology.com]
CC: histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu]
Subject: Re: [Histonet] Paraffin block disposal

That's a good question. I'd like to know the answer myself to that. :)

Sent from my iPhone

 On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta rarba...@csdermatology.com 
 wrote:

 Per CLIA we only need to keep paraffin blocks two years. What is the proper 
 way to dispose of them?

 DISCLAIMER: The information in this message is confidential and may be 
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 message by anyone else is unauthorized. If you are not the intended 
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 unlawful. Please immediately contact the sender if you have received this 
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[Histonet] Adenovirus controls

[Cooper, Brian]

Good morning Histonet, 

It's been about a month since I last sent out an inquiry about adenovirus 
control material, and I struck out badly with only one response!   Does anyone 
have a spare adenovirus block they'd be willing to trade for something else 
they might need?  We have a lot of control material available for special 
stains--Fungus, Gram +/-, AFB, etc . . .  For IHC, we have Eber and TONS and 
TONS of Tonsil!   You can contact me offline if you're more comfortable doing 
so.  Any help would be greatly appreciated.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 
bcoo...@chla.usc.edu 




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Re: [Histonet] Friday Trivia Question: Most specimen on a single case

[Cooper, Brian]

I once saw an entire breast submitted--it was something like 100+ blocks.  

Thanks, 

Brian

-Original Message-
From: Stacy McLaughlin [mailto:stacy_mclaugh...@cooley-dickinson.org] 
Sent: Friday, May 08, 2015 9:49 AM
To: Michael Mihalik; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Friday Trivia Question: Most specimen on a single case

I've never seen that many on one case.  The most I've seen is ~30 (parathyroid) 
and its been many years.
What types of specimens are they?
Stacy

-Original Message-
From: Michael Mihalik [mailto:m...@pathview.com]
Sent: Friday, May 08, 2015 12:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Friday Trivia Question: Most specimen on a single case

Please excuse the trivia query, but we've got a client who somewhat regularly 
creates cases with 100+ specimen.  I think the most I have ever seen is 127.

I'm curious how common this is.  What's the most specimen on a single case 
you've ever seen?

Thanks for your patience and experience.

Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369



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Re: [Histonet] Can't log into Histonet to do anything

[Cooper, Brian]

I got Gayle's email as well, but when I went to Histonet's page though Firefox 
and Google, got the Unable to Connect message when I clicked on a bunch of 
the Monthly hyperlinks.  Same message for everything back until the Histonet, 
November, 2008 hyperlink.  They seem to work prior to this date.  Tried IE as 
well, same result.  

Brian


-Original Message-
From: koelli...@comcast.net [mailto:koelli...@comcast.net] 
Sent: Monday, May 04, 2015 3:55 PM
To: callis, gayle
Cc: Histonet
Subject: Re: [Histonet] Can't log into Histonet to do anything

I got your message Gayle (through Histonet) although haven't heard much at all 
weekend otherwise.  Use IE. 
Ray  in Lake Forest Park, WA 

- Original Message -

From: Gayle Callis gayle.cal...@bresnan.net
To: Histonet histonet@lists.utsouthwestern.edu
Sent: Monday, May 4, 2015 3:21:01 PM
Subject: [Histonet] Can't log into Histonet to do anything 

Dear Histonettters, 

  

At the risk of being pesky, is Histonet having problems.   I generally go to 
Histonet via Firefox/Google and haven't been able to get to the website for two 
days.   I only hope someone out there can even get this message.  I have tried 
finding Marvin Hanna's email address.   

  

Stymied and dead in the water.   I would love to unsubscribe, but not sure 
anyone gets this message. 

  

Gayle Callis 

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[Histonet] FW: ATRT delay in formalin fixation

[Cooper, Brian]

Good afternoon Histonet,

We got a complaint from one of our researchers this morning about loss of 
antigenicity on an FFPE sample. According to the researcher the pERK antigen 
in tissue is labile if not fixed right away, hence trying to find if the 
samples which are negative are truly negative or could be a factor of time to 
fixing.   She's hypothesized that it's likely due to a delay in formalin 
fixation from the time of removal from the patient.

When we perform frozen sections, the tissue that's removed sits in a petri dish 
fresh until the decision is made on how to proceed with the sample.  By the 
time frozen samples are prepared and tissue is set aside for ancillary studies, 
it can be as long as 30 minutes until the remaining tissue is placed in 
formalin.  Have any of you had complaints of antigenicity loss due to this 
delay?  We've only had one researcher make this complaint, and it's only on 
cases of AT/RT.  None of our pathologists have noticed this phenomenon.  Is 
this exclusive for this pERK antigen?

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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[Histonet] Adenovirus controls

[Cooper, Brian]

Good morning Histonet,

Would any of you have any adenovirus control blocks you'd be willing to trade?  
We have real fungus and gram control blocks we can part with (not orange peels 
or Slim Jims :)).

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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[Histonet] RE: Centrifuge for cytospins

[Cooper, Brian]

We make our BAL cytospins directly--no centrifuging first.

Brian

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Tuesday, April 28, 2015 8:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Centrifuge for cytospins

Hi Everyone,

Just asking a quick question for our Cyto department. Are BAL slides 
centrifuged and then made in to cytospin slides or do you just make the 
cytospin slides with no centrifuging?

Thank you,

Jessica Piche, HT(ASCP)



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RE: [Histonet] setting up for staining

[Cooper, Brian]

We use the same setup for our cytology staining. We run a Pap stain about once 
every three weeks. Those dishes and racks are sturdy.

Thanks,

Brian Cooper, HT (ASCP)
Histology Supervisor,
Path  Lab Medicine
Children's Hospital, Los Angeles

Sent from my Galaxy S3, so please forgive any weird typos . . .


-Original Message-
From: Emily Brown [talulahg...@gmail.com]
Received: Friday, 17 Apr 2015, 5:43AM
To: histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu]
Subject: [Histonet] setting up for staining

Hello!

My lab doesn't really do staining (H and E, Masson Trichrome) too often
(maybe once a week), but I'd really like to have a set of dishes with all
of the plastic bins and what not.  Unfortunately, this costs $500 for just
one row.
Has anyone tried the newer set-ups like this
https://us.vwr.com/store/catalog/product.jsp?product_id=4790248

It's remarkably cheaper, but I wonder if that's because it's not as good.
We don't have a lot of money, and convincing my boss to even consider this
will probably be difficult.  I've been using three glass dishes and pouring
reagents in and out of them; it's time to move on to an actual set where we
can store the reagents in the dish.
While I wish we could buy the cool Tissue Tek version (since everyone else
has it), it's not feasible at all considering the cost.

(Also, Ann, stop lurking I know you're reading this!!)

Emily


By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward.

-Chuck Palahniuk, Haunted
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RE: [Histonet] Re: Pam Marcum colleague losing bone sections from slides

[Cooper, Brian]

Have you ever tried blotting your slides dry before putting them into the oven? 
 The veteran who taught me this trick used it on brain tissues from the 
Coroner's office (in the early 80s--don't wanna offend anyone) which were 
grossed very thickly and were always poorly processed.   She never used charged 
slides or additives in her waterbath.  She claimed she was the only one in her 
lab who was allowed to cut this stuff because everyone else's slides had 
tissue loss!  I can tell you from my experience that it works well for toenail 
which is notorious for detaching from slides.  I've used it on many other 
tissues as well.

Anyway, press a slightly moistened clean L'Absorb or paper towel down onto the 
section after microtomy.  You don't want the paper towel soaking wet--just 
damp.  This will effectively wick the section of any excess moisture.  Then 
incubate and stain as usual.  

Good luck.

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Tuesday, April 07, 2015 2:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Pam Marcum colleague losing bone sections from slides

From Pam:  I am currently trying to stain L6 vertebrae from rabbits. 
They
have been decalcified and paraffin processed properly. I've tried cutting at 
both 5 and 10 microns and my tissue is still not sticking to my slides. I know 
my sectioning is fine because I'm successful with every other tissue I've ever 
sectioned and stained. For some reason the bone I'm using won't stick to any 
slides. I was using charged slides and I even tried poly-L-lysine slides, but 
the bone keeps coming up even before I attempted to stain them. I've even tried 
leaving them in the incubator for more than the usual 48-72 hours. I know it's 
possible to do other stains beside HE on bone, but I think my main issue is 
just getting good contact between the tissue and slide. If you have any advice 
or thoughts, I would love to hear them. 

  

I will get the messages to him ASAP. 

  

Pam 

*

 

What was meant by incubator and at what temperature?   It helps to dry
sections FLAT, at 37 to 40C for several days.  Do NOT dry at 60C.   

 

If the sections are not staying on plus charge or poly L lysine coated slides,  
then use chrome gelatin subbing solution in a water bath OR by
pre-subbing clean microscope slides.

 

This is the Chrome gelatin protocol that worked for our huge decalcified bone 
sections and or problem bone sections. 

 

Chrome Gelatin Subbing Solution:  Section/Slide Coating Adhesive

 

0.1 g Chromium Potassium Sulfate (this is toxic.  Collect for proper disposal, 
not down the drain is you pre-sub the slides). 

1.0 g Gelatin:  100 bloom, Sigma.  For large bone sections, use 200 or 300
bloom gelatin, Sigma).   200 and 300 bloom gelatins are very large gelatin
molecules made from pig collagen.  100 bloom is a much smaller molecule than
200 bloom.   Do NOT use household (cooking)  gelatin used for
cooking. Buy the pure gelatins only. 

1 liter Distilled Water

 

Dissolve chromium potassium sulfate and gelatin in hot but not 
boiling water.  Cool subbing solution before use, and store in refrigerator.  
If gelatin gets growth, discard, make new.  A few crystals of Thymol in stock 
subbing solution can help prevent growth. 

 

DO NOT USE PLUS CHARGE SLIDES WITH SUBBING SOLUTION.   GELATIN COATS OVER A
PLUS CHARGE COATING AND NEGATES THE POSITIVE CHARGE.  

 

For presubbing glass slides, wash these by dipping in acetone, air dry before 
using the pre-subbing protocol to get rid of any greasy/oily residues
on glass surface.   If you put the subbing solution in a water bath,
uncoated,  glass slides will work fine without further washing.

 

You can do either of the following: 

 

1.Add 10 ml subbing solution to a warm water bath for paraffin
sections. Then mount sections onto the cleaned glass slide, drain, and air dry, 
store in a cool, dry place. 

2.Dip acetone washed, dry slides into subbing solution, air dry,
and store in a dust free area.  Box subbed slides and store until needed.  

 

If you get background staining with hematoxylin (hematoxylin stains gelatin) 
then dip  pre-subbed slides in NBF ~10 times, rinse with distilled water, air 
dry and store slides.  The aldehyde fixative cross links the gelatin to some 
degree, but still allows section to adhere without annoying background 
staining.  

 

Pick up sections from water bath drain and lay flat to dry at 40C for
several days.   You will not need extra subbing solution in the water bath
if using presubbed 

[Histonet] RE: Cutting of Paraffin Blocks

[Cooper, Brian]

Completely agree.  Give them practice tissue blocks because it's expected that 
they WILL destroy them during the early stages of their learning process.  

If I can go one further here--when these students have demonstrated acceptable 
microtomy technique (and when you feel comfortable), be sure to teach them how 
to realign their microtome's orientation head/specimen clamp to the surface of 
blocks that have been previously cut.I cannot tell you how many histotechs 
I've met in my career who didn't possess this skill and simply refaced these 
blocks.   One even yelled at the Biomedical Maintenance tech when he serviced 
her microtome and readjusted the orientation!   

I have a student in our lab right now, and tasked her with providing me 5 HE's 
from each of the practice blocks I gave her.  Here's the catch.  In between 
each section that she collects, she is supposed to completely misalign the 
specimen head, then realign to the surface of the block.  I told her that all 
five HE's from each block should look relatively similar by the time she's 
done, and that there should be no appreciable tissue loss.  While this might 
seem like a torture test for beginners, it will only serve them (and ultimately 
the patients they serve) so well in the future. 

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Wednesday, March 25, 2015 9:49 AM
To: Adesupo, Adesuyi (Banjo); 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Cutting of Paraffin Blocks

Heck no, crack that whip!  Give them practice tissue that isn't important so 
they won't be afraid of ruining something.

Laurie Colbert, HT (ASCP)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adesupo, 
Adesuyi (Banjo)
Sent: Wednesday, March 25, 2015 9:26 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Cutting of Paraffin Blocks

Hi,
   How are you guys doing? I hope you are all doing well. Please  I have two 
histology trainees and  they have spent more than six months in the histology 
lab and they cannot cut blocks yet. They are comfortable with filing slides and 
blocks.
But I have been telling them that they have to start cutting now. My question 
now is am I too hard on them?

Best regards,
Adesupo Adesuyi
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[Histonet] Pneumatic Tube Delivery System

[Cooper, Brian]

Dear Histonetters,

For those facilities that have a pneumatic tube system in use, do any formalin 
fixed samples get delivered in this manner?   The vast majority of our samples 
will not be, for obvious reasons.  But there has been some discussion of 
combining bone marrow cores and aspirates and tubing them at the same time.  
The specimens will need to be physically separated of course because of the 
potential for damage of the aspirate/smear material due to exposure to formalin 
fumes.  Have any of you crossed this bridge?  I'd love to hear your 
experiences/concerns . . .

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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RE: [Histonet] Controls:

[Cooper, Brian]

I've used moldy orange peels as a GMS/PAS-F control in the past.  Just saved 
the peels in a plastic bag over the weekend, then processed as usual.  They 
worked beautifully!

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Thursday, March 05, 2015 7:21 AM
To: Jb; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:

We have successfully used hamburger meat to make Gram controls.  As far as GMS 
controls go, we ran across a post from someone smearing cream cheese onto lung 
tissue and letting it sit for a couple of days, fixed and processed it and were 
able to demonstrate Aspergillus by GMS.  My fungus control stocks are low so I 
was actually planning to try this with some beef lung.  I haven't heard of the 
Slim Jim method before.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Wednesday, March 04, 2015 1:05 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Controls:

Off the wall question, I have been told that slim jims (pepperoni stick) at the 
gas station can be processed and used as good gram controls. Has anyone done 
this and do they work for GMS also?

Thank you,

Sent from my iPhone
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[Histonet] RE: cilia for EM

[Cooper, Brian]

Will respondents please be sure to click reply all to this post?  We're 
having the same issue here.  Namely, remnants of the sample collection brush 
remain in the vial and damage our diamond knives.  Any advice would be greatly 
appreciated.
  
Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A
Sent: Thursday, February 26, 2015 9:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cilia for EM

Does anyone have a good technique/tool for cilia biopsy collection that we 
could share with our physicians.  We have been having issues with poor quality 
specimens.
Thanks,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.al...@providence.orgmailto:lacie.al...@providence.org


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[Histonet] Frozen tissue/OCT Vials

[Cooper, Brian]

It's almost Friday Histonetters!

What kinds of containers are you storing your OCT embedded samples in?  We're 
currently using snap top vials for all of our frozen samples that have a 
tendency to pop their lids when removed from our LN2 freezers!   Wondering if 
there are some type of screw top vials that will suit our purposes.  I know we 
will likely have to change the boxes we store our vials in as well, but that's 
par for the course.  Any help will be greatly appreciated!

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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[Histonet] RE: Cassette Labeler

[Cooper, Brian]

Leica's IPC cassette printers are workhorses.  This is the only type I've ever 
had in the labs I've worked in, so I can't comment on any others.

Brian


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Tuesday, January 27, 2015 8:35 AM
To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; 'Debbie Granato'; 
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cassette Labeler

I would look into any of them. They work great.

-Original Message-
From: Sanders, Jeanine (CDC/OID/NCEZID) [mailto:j...@cdc.gov] 
Sent: Tuesday, January 27, 2015 10:34 AM
To: Mike Pence; 'Debbie Granato'; histonet@lists.utsouthwestern.edu
Subject: RE: Cassette Labeler

Sakura also has a similar system

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Tuesday, January 27, 2015 11:32 AM
To: 'Debbie Granato'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cassette Labeler

Look at the SIideMate and PrintMate from Thermofisher. This is what we started 
with then intergraded into our LIS this year.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debbie Granato
Sent: Tuesday, January 27, 2015 10:28 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Labeler

Our lab is currently looking into purchasing a cassette and slide labeler.

We are a small lab and are looking for a stand- alone unit with the flexibility 
to be used with the computer or bar code scanner at a later date.

I would appreciate any feedback or suggestions for any model that may fulfill 
our needs.

Thank you,

Debbie Granato HT(ASCP)


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[Histonet] RE: Sakura slide racks

[Cooper, Brian]

You can get them from Fisher Scientific.  

http://www.fishersci.com/ecomm/servlet/itemdetail?itemdetail=%27item%27storeId=10652productId=13307075catalogId=29104matchedCatNo=NC0046742fromSearch=1searchKey=sakura+4768highlightProductsItemsFlag=YendecaSearchQuery=%23store%3DScientific%23nav%3D0%23rpp%3D25%23offSet%3D0%23keyWord%3Dsakura%2B4768%23searchType%3DPROD%23SWKeyList%3D%5B%5DxrefPartType=Fromsavings=0.0xrefEvent=1421876996159_0searchType=PRODhasPromo=0
 

Thanks, 

Brian

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Wednesday, January 21, 2015 1:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sakura slide racks

Histonetters!!

I am looking for some replacement racks that hold 20 slides and that fit in the 
Sakura Tissue-Tek film coverslipper. For some reason our ordering department 
doesn't want us to/let us order directly from Sakura.

Thanks

Claire

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RE: [Histonet] RE: specials stainers

[Cooper, Brian]

We have a pair of each of these in our lab right now on demo (only until I can 
get the paperwork signed later this week). Dako takes it hands down!

Thanks,

Brian Cooper, HT (ASCP)
Histology Supervisor,
Path  Lab Medicine
Children's Hospital, Los Angeles

Sent from my Galaxy S3, so please forgive any weird typos . . .


-Original Message-
From: Houston, Ronald [ronald.hous...@nationwidechildrens.org]
Received: Tuesday, 20 Jan 2015, 5:39AM
To: 'Mitchell, Janice A' [mitchel...@email.chop.edu]; 
histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu]
Subject: [Histonet] RE: specials stainers

DAKO wins hands-down, without any reservation

Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.comhttp://www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.NationwideChildrens.org

One person with passion is better than forty people merely interested.
~ E.M. Forster



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell, 
Janice A
Sent: Tuesday, January 20, 2015 6:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] specials stainers

Good Morning,
We are looking for an automatic stainer for special stains.   Ventana vs Dako?  
Thoughts?
Thanks for any input, Janice Mitchell
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[Histonet] Paraffin Temperature Checks

[Cooper, Brian]

For those of you out there that are fortunate enough to have tissue processors 
or embedding centers that are used less often than daily-like maybe even weekly 
(or even less frequently,) how often are you checking and documenting the 
paraffin temperatures on said pieces of equipment?  For our daily equipment, 
it's no big deal obviously.  But we have a research processor and embedding 
center that doesn't get used often-sometimes for a week or two, and if we're 
not touching the machine, it seems overkill to document paraffin temps daily.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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[Histonet] RE: PASD muscle stains

[Cooper, Brian]

Same here. 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Prasad 
(SCHN)
Sent: Tuesday, January 06, 2015 2:58 PM
To: 'Tiffany Passaro'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: PASD muscle stains

Usually we do the DiPAS stain on muscle frozen section. Air dried for 10 
minutes. Don't use any fixatives :)


Linda Prasad, MSc, BSc

Senior Scientist | Histopathology
t: 02 9845 3306 | f: 02 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au
m: 0425 314 267
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead 
NSW 2145, AUSTRALIA



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tiffany Passaro
Sent: Tuesday, 6 January 2015 10:23 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] PASD muscle stains

Greetings,


I am looking for fixatives that others are using in their labs 
for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 
10% NBF. Thanks in advance for any info on this.

Tiffany
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[Histonet] RE: Formic acid disposal?

[Cooper, Brian]

Our waste disposal company has us combine our formic acid waste with our 
special stain/HE stain waste.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Carole
Sent: Monday, January 05, 2015 5:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formic acid disposal?

Hello everyone and Happy New Year,

Can anyone who is using formic acid in their lab tell me how they 
handle/dispose of it after use?  I realize there is some variability by region, 
but any help would be appreciated.  Thanks in advance.

Carole Johnson
Carole Johnson, HT(ASCP)cm
New Mexico Department of Agriculture
Veterinary Diagnostic Services
505.383.9299

To understand is to stand under, which is to look up, which is a good way to 
understand




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RE: [Histonet] Warthin Starry on Dako Artisan

[Cooper, Brian]

Hi, 

We've had really good results with pretreatment in 1% Uranyl Nitrate, as well 
as with 1% Uranyl Acetate in Acetate Buffer--both at room temperature, and at 
60 degrees C for 10 minutes.  We've also tried zinc formalin, as well as 
Spirochete Sensitizer--a commercially available product, but we've only 
obtained extremely faint staining of spirochetes with the latter two.   Our 
sections are cut at 4 µm.   
 
Thanks, 

Brian

-Original Message-
From: Sanders, Jeanine (CDC/OID/NCEZID) [mailto:j...@cdc.gov] 
Sent: Wednesday, December 24, 2014 5:13 AM
To: Cooper, Brian; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Warthin Starry on Dako Artisan

What protocol with uranyl nitrate do you use? Dako told us to use zinc formalin 
as a sensitizer but it doesn't seem to make much of a difference. Thanks!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, December 23, 2014 11:18 AM
To: Cooper, Brian; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Warthin Starry on Dako Artisan

Try a 0.10% solution of phosphotungstic acid.René J.  

 On Monday, December 22, 2014 6:39 PM, Cooper, Brian 
bcoo...@chla.usc.edu wrote:
   

 Happy Holidays Histonetters!

Has anyone out there successfully stained spirochetes with Dako's Artisan 
Warthin-Starry kit, without first pretreating the sections with uranyl nitrate? 
 With offline uranyl nitrate pretreatment, spirochetes stain brilliantly!  But 
everything else we've tried leaves them invisible . . .

Any guidance would be greatly appreciated.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357    Pager: 213-209-0184
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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[Histonet] RE: Leder stain

[Cooper, Brian]

Never done the stain myself, but have you tried Poly Scientific?  Check out 
this link . . . 

http://www.polyrnd.com/products/reagent-assembly-kits/conventional/leder-stain-kit.aspx
 

Thanks, 

Brian


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Histology
Sent: Tuesday, December 23, 2014 9:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leder stain

Hi All,

Does anyone know where I can find the reagents to do the Leder stain (Naphthol 
AS-D Chloroacetate Esterase)?  We usually get a kit from Sigma but they have 
been out since September.

Thanks in advance,

Mehndi Helgren

Dominion Pathology Labs
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[Histonet] Warthin Starry on Dako Artisan

[Cooper, Brian]

Happy Holidays Histonetters!

Has anyone out there successfully stained spirochetes with Dako's Artisan 
Warthin-Starry kit, without first pretreating the sections with uranyl nitrate? 
  With offline uranyl nitrate pretreatment, spirochetes stain brilliantly!  But 
everything else we've tried leaves them invisible . . .

Any guidance would be greatly appreciated.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu



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RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek

[Cooper, Brian]

Not sure if it's the same scenario, but this happened to us once about a dozen 
years ago on an older version of a VIP.  Luckily, I haven't seen this again!  

One of our lab assistants loaded a basket of formalin-fixed tissues onto a 
processor that hadn't yet been through the clean cycle.  The machine wouldn't 
let him start the process, so he took the basket out and put it back into 
formalin to wait while he ran the clean cycle.  For that brief moment that the 
basket was in the retort, a bunch of residual formalin drained off the tissues 
and basket and mixed in with the residual molten paraffin that was left in the 
bottom of the retort.  Anyway, after he ran the clean cycle he started the run 
as usual.  8 hours later, our blocks smelled like formalin when we cut them, 
and they were all mushy in texture.   

So Sakura told us that whenever you run the clean cycle, the VIP (again, it was 
a really old model and I'm not sure if the technology is still the same) will 
attempt to draw back into the last station that was used, any residual reagent 
that was left in the retort before proceeding to the next reagent.  Since the 
last reagent had been paraffin, anything in the retort (including the newly 
mixed in formalin) went back into the last paraffin chamber on the VIP.  As 
such, the next basket of tissues were infiltrated with formalin-infused 
paraffin in the very last processing step.   If I recall, we had to reprocess 
almost all of those blocks.  

Hope this helps.   

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P
Sent: Monday, November 24, 2014 10:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue 
tek

Dear all
After processing in vip 6 the last paraffin has a strong odour of formalin

Have anybody experienced the same
Any ideas why it is happening.

Arun
Kuwait
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RE: [Histonet] Specimen numbering systems

[Cooper, Brian]

Same here.

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Children's Hospital, Los Angeles 
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joelle Weaver
Sent: Friday, November 21, 2014 11:30 AM
To: Willis, Donna G.; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Specimen numbering systems

Numeric for the Specimen and Alpha for the Block.
 


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
 From: donna.wil...@baylorhealth.edu
 To: histonet@lists.utsouthwestern.edu
 Date: Fri, 21 Nov 2014 19:02:56 +
 Subject: [Histonet] Specimen numbering systems
 
 Can large facilities of more than 500 beds please let me know how they are 
 numbering their Surgical specimens.  Alpha for the Specimens and numeric for 
 the Block or Numeric for the Specimen and Alpha for the Block.
 
 Thanks,
 
 Donna Willis, HT/HTL(ASCP)
 Anatomic Pathology Manager
 
 Baylor University Medical Center
 3500 Gaston Ave|Dallas, Texas  75246
 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com
 
 
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RE: [Histonet] Tissue falling off positive slides

[Cooper, Brian]

We bake ours @ 75-80 degrees for 15 minutes in an offline oven, prior to 
automated staining.  Just a habit I picked up years ago, but I always lean the 
slide racks diagonally against the side of the oven when they're baking, rather 
than having them sit entirely upright.  They seem to drain any residual water 
more completely that way.  We've used Fisher's positive charged slides for 
years and haven't really had a problem with tissue loss on HE.  I think your 
baking temp and time may be a little low and short, respectively. 

Good luck.

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Thursday, November 06, 2014 8:36 AM
To: Pardue, Judith
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue falling off positive slides

Make sure yr sections r airdryed completely before u bake thm 

Sent from my iPhone

 On Nov 6, 2014, at 10:34 AM, Pardue, Judith judith_par...@memorial.org 
 wrote:
 
 WE are having trouble with tissue coming off our he slides. Our heater is 
 set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue 
 positive slides.
 
 Judith Pardue
 CHI Memorial
 Chatt. Tn. 37343
 judith_par...@memorial.org
 
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[Histonet] RE: undercounter crushed ice makers?

[Cooper, Brian]

We had one at my previous institution.  Most of the techs (including me) 
preferred the large, solid blocks of ice to the crushed stuff, except when it 
came time to make margaritas.  Then we were all over the crushed stuff!  

The solid blocks hold moisture at the surface better than the crushed ice, and 
they stay colder longer.  Crushed ice doesn't hold a flat surface when you add 
water to it (even when you compact it), and we seemed to constantly be pushing 
the blocks down into the slushy abyss.  Maybe I didn't give it enough of a try, 
and I have been accused (rightfully so) of being set in my ways!  Not an issue 
here though--we have a freezer full of big ol' blocks of ice . . . 

Happy Halloween Histonet!!!

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Friday, October 31, 2014 3:16 PM
To: Histonet
Subject: [Histonet] undercounter crushed ice makers?

Does anyone use an undercounter crushed ice maker they are happy with? We are 
looking into this for microtomy ice trays.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org

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RE: [Histonet] Paraffin Used

[Cooper, Brian]

Hi Bill, 

We infiltrate with Paraplast, and embed with Paraplast Extra.

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Thursday, October 23, 2014 11:48 AM
To: histonet
Subject: [Histonet] Paraffin Used

What paraffin do you use in your lab? Do you use different type for processing 
and embedding? Looking to investigate the top used paraffins for a workshop I 
am putting together.

Sent from my iPhone
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RE: [Histonet] white plastic scrapers

[Cooper, Brian]

You know what works better? A small, metal drywall taping/mud knife-maybe 3-4 
inches. They're more flexible than the plastic scrapers, and I think they 
actually pick up more paraffin per pass. You can pick these up at any hardware 
store.

Thanks,

Brian Cooper, HT (ASCP)
Histology Supervisor,
Path  Lab Medicine
Children's Hospital, Los Angeles

Sent from my Galaxy S3, so please forgive any weird typos . . .


-Original Message-
From: Betsy Molinari [bmolin...@texasheart.org]
Received: Tuesday, 14 Oct 2014, 5:08AM
To: Histonet@lists.utsouthwestern.edu [Histonet@lists.utsouthwestern.edu]
Subject: [Histonet] white plastic scrapers

Hi all,
Where can I purchase the whit plastic scrapers used to scrape the paraffin off 
embedding centers and other surfaces. I believe ours went out with the trash.
Thanks.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston,TX 77030
832-355-6524 (lab)
832-355-6812 (fax)



http://www.texasheart.org



Betsy Molinari
Senior Histology Research Technician
832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | 
www.texasheart.orghttp://www.texasheart.org



6770 Bertner Ave., MC 1-283, Houston, TX 77030



[Texas Heart 
Institute]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI
 News] [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute  [THI 
on Flicker] http://www.flickr.com/photos/texasheart/sets/  [THI on Google] 
https://plus.google.com/u/0/118043615690351997044/posts   [THI on Pinterest] 
http://pinterest.com/texasheartinst/  [THI on Twitter] 
http://twitter.com/Texas_Heart  [THI on You Tube] 
http://www.youtube.com/TexasHeartInstitute


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[Histonet] RE: Gram staining

[Cooper, Brian]

We use the McDonald's Gram Stain kit from American Master Tech, which employs 
the use of a Modified Tartrazine counterstain.  Both our docs and techs love 
our Gram stains . . .  

Here's the link to the product info.

http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesitem=KTMGS 

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela 
K.
Sent: Thursday, October 09, 2014 11:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gram staining


Our new Pathologist would like to see more yellow in the background of our Gram 
stain.  We use picric acid-acetone currently, which looks more pink than yellow 
most times. So  I'm wondering if a method using a Tartrazine counterstain would 
be better.
Any thoughts?


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RE: [Histonet] slide id

[Cooper, Brian]

How does Vantage work with blocks and slides that were in your institution 
prior to the implementation of Vantage?  Suppose you needed to send materials 
to another institution for further testing?  Do you generate new barcoded 
labels and affix them to the materials prior to sending them out (for tracking 
purposes)? 

Thanks, 

Brian


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Friday, October 03, 2014 1:21 PM
To: Rathborne, Toni
Cc: Histonet@lists.utsouthwestern.edu; Tapper, Sheila J.
Subject: Re: [Histonet] slide id

Vantage works great. We use it to track all blocks and slides as they move in 
and out of the core lab and in and out of archive. You must bar code and make a 
decision on cassette printers. Techs scan their own bar code to log on the 
system.

Sent from my iPhone

 On Oct 3, 2014, at 1:17 PM, Rathborne, Toni toni.rathbo...@rwjuh.edu wrote:
 
 How do you like Vantage? Have you experienced any problems with it, and is it 
 easy to use?
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM 
 DESALVO
 Sent: Friday, October 03, 2014 4:02 PM
 To: Tapper, Sheila J.
 Cc: Histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] slide id
 
 We use Vantage Quality and Tracking system and the tech is captured for 
 embedding, microtomy and staining
 
 Sent from my iPhone
 
 On Oct 3, 2014, at 12:55 PM, Tapper, Sheila J. 
 sheila.tap...@essentiahealth.org wrote:
 
 We have our techs fast process the slides that they cut - so the tech is 
 documented in the processing history... same for embedding. 
 
 Sheila 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
 Sent: Friday, October 03, 2014 2:51 PM
 To: 'anita'; Histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] slide id
 
 We do.
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita
 Sent: Friday, October 03, 2014 2:50 PM
 To: Histonet@lists.utsouthwestern.edu
 Subject: [Histonet] slide id
 
 just wondering if techs are putting their id on the slides that they cut, 
 that way if a mistake is made with labeling the tech that cut it is 
 identified.
 
 thanks for your input,
 
 anita dudley
 
 providence hosp
 
 mobile alabama 
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RE: [Histonet] slide id

[Cooper, Brian]

Thanks Tim.  I think we're going the ABT route as well with our next CoPath 
upgrade.  We went to Ventana last year for their 360 tour, and saw all that 
Vantage has to offer.  One of my good friends is a Vantage Implementation 
Manager, so I'm sure our decision to go with ABT  will drive him batty!  

Happy Friday!!!  

Brian

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Friday, October 03, 2014 3:37 PM
To: Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] slide id

Brian, we use Copath ABT, not Vantage, but the principle is the same. We use 
the old non-barcoded system for older block/slide sendouts. There is no way to 
barcode old blocks, unless you want to enter it as a consult, and that entails 
giving it a new number, which would just confuse things.

It will take a few years but eventually we'll be sending out mostly barcoded 
items. This is just one of the things you have to work with when changing over 
to something new. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center San Francisco, CA

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message.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Friday, October 03, 2014 1:26 PM
To: WILLIAM DESALVO; Rathborne, Toni
Cc: Histonet@lists.utsouthwestern.edu; Tapper, Sheila J.
Subject: RE: [Histonet] slide id

How does Vantage work with blocks and slides that were in your institution 
prior to the implementation of Vantage?  Suppose you needed to send materials 
to another institution for further testing?  Do you generate new barcoded 
labels and affix them to the materials prior to sending them out (for tracking 
purposes)? 

Thanks, 

Brian


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Friday, October 03, 2014 1:21 PM
To: Rathborne, Toni
Cc: Histonet@lists.utsouthwestern.edu; Tapper, Sheila J.
Subject: Re: [Histonet] slide id

Vantage works great. We use it to track all blocks and slides as they move in 
and out of the core lab and in and out of archive. You must bar code and make a 
decision on cassette printers. Techs scan their own bar code to log on the 
system.

Sent from my iPhone

 On Oct 3, 2014, at 1:17 PM, Rathborne, Toni toni.rathbo...@rwjuh.edu wrote:
 
 How do you like Vantage? Have you experienced any problems with it, and is it 
 easy to use?
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
 WILLIAM DESALVO
 Sent: Friday, October 03, 2014 4:02 PM
 To: Tapper, Sheila J.
 Cc: Histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] slide id
 
 We use Vantage Quality and Tracking system and the tech is captured 
 for embedding, microtomy and staining
 
 Sent from my iPhone
 
 On Oct 3, 2014, at 12:55 PM, Tapper, Sheila J. 
 sheila.tap...@essentiahealth.org wrote:
 
 We have our techs fast process the slides that they cut - so the tech is 
 documented in the processing history... same for embedding. 
 
 Sheila
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike 
 Pence
 Sent: Friday, October 03, 2014 2:51 PM
 To: 'anita'; Histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] slide id
 
 We do.
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita
 Sent: Friday, October 03, 2014 2:50 PM
 To: Histonet@lists.utsouthwestern.edu
 Subject: [Histonet] slide id
 
 just wondering if techs are putting their id on the slides that they cut, 
 that way if a mistake is made with labeling the tech that cut it is 
 identified.
 
 thanks for your input,
 
 anita dudley
 
 providence hosp
 
 mobile alabama

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[Histonet] RE: Automated Special Stainer...

[Cooper, Brian]

Dako Artisan all the way!   I've used both systems (the Artisan in my old 
institution).  We currently have a few of the new Benchmarks on demo right now. 
 Thus far, we're not that impressed.  Frequently, there's variability on the 
silver stains (even in the same run,) and adjusting timing is nowhere near as 
flexible as on the Artisan.  Even if you take away the half hour for online 
deparaffinization, the stains take significantly longer on the Benchmarks (than 
the old Nexes we are still running in our lab).   Oh yeah, one more thing.  The 
reagent tray capacity on the Artisan is about twice what it is on a Benchmark.  
You'll need two Benchmarks to do what one Artisan does, TATs being equal.   
  

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sarah.dys...@stdavids.com
Sent: Wednesday, September 17, 2014 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated Special Stainer...

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's 
North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

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[Histonet] RE: Automated Special Stainer...

[Cooper, Brian]

I guess TAT's being equal is a relative term, depending upon your workload.  
For our purposes, it's not slide capacity that poses a problem--it's reagent 
capacity.  We've never loaded 50 special stains at any one time--we typically 
don't even have half that amount in one day!  

So here's a scenario common to the workload at our institution.  For our liver 
panels, we run Iron, Trichrome, Retic, PAS with and without Digestion.  That 
pretty much maxes out the reagent capacity on the Benchmark.  Sure, we can load 
up to 20 of these onto the Benchmark.  But as is so frequently the case, we 
also have just 1 GMS that also needs to be stained!  This is where the second 
Benchmark comes into play, or we'll have to stain by hand.   One Artisan can 
handle this at the same time, and if memory serves (disclosure--it's been about 
2 years, and the model I used didn't have online deparaffinization), it didn't 
add all that much time to the process.  It was certainly faster than waiting 
for the machine to complete, and start another run.   For our institution, it's 
either going to be 2 Artisans or 2 Benchmarks.  The Artisans will give us some 
greater flexibility due to the greater reagent capacity.  

Brian


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, September 17, 2014 10:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Brian wrote: ... one more thing.  The reagent tray capacity on the Artisan is 
about twice what it is on a Benchmark.

Are the TAT's equal? When we loaded up our old artisan with slides (50) and 
full of reagents it took over 5 hours to finish - way past our deadline. So 
nobody wanted to use it that way so the techs resisted and wanted to do manual 
to get the slide out. We ended up putting only a few stains on it.

We  are looking at doing specials the way we do immunos - on demand all day 
rather than in one batch in the AM. 

It seemed to me that the 20-slide units from Ventana allow small batches and 
run in parallel or allow staggered use. Buying a 50-slide capacity instrument 
and then putting only 20 slides at a time on it seems odd somehow.


We looking at each of these as well and not sure yet which would serve us 
better. We do about 60-80 specials slides per day, and 13 stains give us 90% of 
our volume. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center San Francisco, CA

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Wednesday, September 17, 2014 10:33 AM
To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Automated Special Stainer...

Dako Artisan all the way!   I've used both systems (the Artisan in my old 
institution).  We currently have a few of the new Benchmarks on demo right now. 
 Thus far, we're not that impressed.  Frequently, there's variability on the 
silver stains (even in the same run,) and adjusting timing is nowhere near as 
flexible as on the Artisan.  Even if you take away the half hour for online 
deparaffinization, the stains take significantly longer on the Benchmarks (than 
the old Nexes we are still running in our lab).   Oh yeah, one more thing.  The 
reagent tray capacity on the Artisan is about twice what it is on a Benchmark.  
You'll need two Benchmarks to do what one Artisan does, TATs being equal.   
  

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
sarah.dys...@stdavids.com
Sent: Wednesday, September 17, 2014 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Automated Special Stainer...

Opinions on Ventana versus Dako...Go!

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's 
North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

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