[Histonet] p16
Happy Wednesday Everyone Has anyone been having consistency problems with their p16 staining since MTM has been taken over by Ventana? One day the procedure works another day it doesn't. Same procedure, no variation in the temperature on pretreatment, run on the Dako 48, with no errors. Every other IHC works consistently but the p16, anyone have any thoughts? I do make the DAB fresh daily as suggested in the instructions, it is the same kit that has been running. The only thing I can think of is we do sometimes run the p16 twice a day without making fresh DAB, but the instructions say it can be used within a 24 hour period. This problem is driving me crazy! It just doesn't make any sense. Any help would be appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] p16
Yes we monitor the temp and humidity. Retrieval is made fresh for every run. We run the PT for the p16 in a water bath since we only run a few slides at a time, but the water bath temp in also monitored. I should say that there is some staining in these slides just not what the control usually looks like. If we repeat the slides they do stain correctly. There is no difference in the slide prep or the staining procedure. Any ideas. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: Ross Benik [mailto:r...@premierlab.com] Sent: Wednesday, February 06, 2013 1:26 PM To: Cynthia Pyse Subject: RE: [Histonet] p16 Do you monitor the temperature and humidity inside the Link48? How often do you prepare your retrieval solution/are you using the PT mods? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 06, 2013 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Happy Wednesday Everyone Has anyone been having consistency problems with their p16 staining since MTM has been taken over by Ventana? One day the procedure works another day it doesn't. Same procedure, no variation in the temperature on pretreatment, run on the Dako 48, with no errors. Every other IHC works consistently but the p16, anyone have any thoughts? I do make the DAB fresh daily as suggested in the instructions, it is the same kit that has been running. The only thing I can think of is we do sometimes run the p16 twice a day without making fresh DAB, but the instructions say it can be used within a 24 hour period. This problem is driving me crazy! It just doesn't make any sense. Any help would be appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] problem with 88305 reimbursement
Hi Histonetters We are currently having a problem with our Medicare reimbursement on the tech component of the 88305. If the patient has been seen at a hospital, either in-patient or out- patient, we are told by Medicare that we have to contract with that hospital to bill for the 88305. The biopsy was not done at the hospital but in a doctor's office. According to Medicare if there the same date of service it can only be billed through the hospital. Medicare is calling it consolidated billing. I can't see how the hospital can bill for something that was done in the doctor's office then sent to an independent lab. Is anyone else having this problem? How are you handling it with Medicare? Any help would be appreciated. Thanks in advance Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] problem with 88305 reimbursement
It's not a SNF but an actual hospital. These are outpatient that go to the hospital for any service. Example, one patient went to a clinic for a vaccination, another went for blood work. None of which has anything to do with the biopsy from the doctor's office. Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: Brendal Finlay [mailto:brendal.fin...@medicalcenterclinic.com] Sent: Tuesday, January 29, 2013 2:32 PM To: Cynthia Pyse Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] problem with 88305 reimbursement Is it a skilled nursing facility? If a patient is in an SNF (for rehab or other reasons) then Medicare will not pay. The testing facility has to contract with the SNF for payment. I have not seen this with a hospital, though. Brendal Finlay, HT (ASCP) On Jan 29, 2013, at 12:51 PM, Cynthia Pyse cp...@x-celllab.com wrote: Hi Histonetters We are currently having a problem with our Medicare reimbursement on the tech component of the 88305. If the patient has been seen at a hospital, either in-patient or out- patient, we are told by Medicare that we have to contract with that hospital to bill for the 88305. The biopsy was not done at the hospital but in a doctor's office. According to Medicare if there the same date of service it can only be billed through the hospital. Medicare is calling it consolidated billing. I can't see how the hospital can bill for something that was done in the doctor's office then sent to an independent lab. Is anyone else having this problem? How are you handling it with Medicare? Any help would be appreciated. Thanks in advance Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] her 2 neu, CISH vs FISH
Good afternoon histonetters We are currently investigating bringing Her 2 Neu CISH staining into our lab. FISH staining has too many logistics nightmares for me to consider, not to mention the cost of purchasing digital scanning equipment. Our pathologist are off site in 5 different hospitals. Does anyone know if there is a sensitivity issue between fluoresce and chromogen staining? Are oncologists receptive to having the Her 2 Neu confirmed by chromogenic ISH as opposed to fluoresce ISH? Anyone performing Her 2 Neu by CISH, what, if any, issues have you encountered. Any help and information would be appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] moleculer testing
Happy Monday Histonetter Who out there in Histoland is doing the testing for BRAF, KRAS, ALK, and HER2neu and what testing methods are you using. I prefer not to use FISH. Would rather have a permanent record of the slides. Any information is welcome. Have a great week. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION
Yes you are. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Friday, December 07, 2012 12:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION Hi All: First, thank you for all your feedback on the processors. I site-visited a VIP6 at a local hospital From my understanding the VIP6 can rotate absolute alcohol and xylene (using the bulk reservoirs), as well as the paraffin stations, but it cannot rotate other concentrations of alcohol based on hydrometer readings. Am I correct in this appraisal? Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thanks
I want to thank everyone who responded to my chemical disposal question. I now have adequate ammunition to combat my general manager's lack of knowledge. He seems to think I don't know what I am talking about. Thanks again for the backup it is appreciated. Have a great weekend. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Waste drum labeling
I would also be interested in the answers to this. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, November 28, 2012 1:16 AM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste drum labeling For EPA standards, how do you label your waste drums? I have one 55 lb drum that we only pour ventana waste in. What numbers do you write in the diamond to cover all the kits that you stain with? 2 reps from EPA came by my lab yesterday and said i needed an emergency phone list by our phone?? Does anyone have the checklist for EPA that small labs should follow? From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Cristi Rigazio [cls71...@gmail.com] Sent: Tuesday, November 27, 2012 3:01 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Elizabeth Cameron Subject: Re: [Histonet] Uneven Staining As most other bases have been covered, do you leave space between the slides in the rack? Once in a blue moon we had stains from the same rack that differed in intensity, after much troubleshooting we started putting space between the slides and we haven't had the problem since. Just an idea. Cristi Sent from my iPhone On Nov 27, 2012, at 12:24 PM, Rene J Buesa rjbu...@yahoo.com wrote: This is quite a puzzling situation. You have ruled out dewaxing (that was my first idea of cause) but I do not think that soaking will be the problem because the sections are fixed and infiltrated with paraffin, so there is no possibility of diluting in water. Thick/thin sections in a series is a very rare occurrence so I am also at a loss with this problem. Weak staining solutions could cause it but all sections in a series will be equally weak. Just in case, since you have covered the dewaxing, issue do the following: do not leave the sections too long in the water bath and dry them in the oven as soon as they are drained. René J. From: Elizabeth Cameron elizabeth.came...@jax.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, November 27, 2012 1:35 PM Subject: [Histonet] Uneven Staining About a year ago, I posted about issues with staining mouse kidney sections (see below for original post). We realized that part of the problem was the sections drying during staining, which has been resolved, but we are still having some issues with some slides staining well and others being very pale. This time it seems to be consistent across the slide, so I know it is not a drying issue. They are serial sections, so they are all cut together and stained together, and they are from the same animal. I don't believe deparaffinization is an issue - we have tried longer times with fresh solutions and it doesn't make a difference. We have also tried re-staining the slides after letting them sit in xylene overnight, and there is no difference in the staining. I am now thinking that it may be related to how long the sections soak or how long they sit on the waterbath. They are NBF fixed, and there is a fine line between soaking well for hydration and oversoaking, resulting in swelling. Has anyone experienced anything like this? Any other ideas? I am at a loss, and I'd really like for this to work! Thanks, Liz We are doing a Hale's colloidal iron stain (no counterstain) on serial sections of mouse kidneys. We are staining an entire kidney at a time (about 90-150 slides), and after many successful runs, we are now finding some slides in each batch with very uneven staining. Half of a section will stain as it should, and the rest of the section is very pale. It seems to be in a similar area from one section to the next, but not exactly the same area. It does not look like a deparaffinization issue. There may be two or three slides in a row like this, or just one section on a slide, followed by 30-40 that look fine. The sections are 6 microns. Any ideas on why this might be happening? Thanks! The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] chemical disposal
Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous waste. I disagree, it is a chemical and needs to be disposed in the chemical hazardous waste. What is everyone else doing to dispose of DAB. We are located in NY, I do have those regs. Thanks in advance for any and all help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Processors
Rhonda I have both a Thermo-Fisher Excelsior and a Sakura VIP6. Both work well but I prefer the Excelsior. There is less hands on time for changing reagents, flushing and cleaning. I did have problems at first with the VIP. Since Sakura replaced the gate valve all has been great. If I need to replace either machine I would purchase the Excelsior. This is just my opinion, I'm sure other out there may disagree with me. Everyone out there in Histoland have a safe, healthy Thanksgiving. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gregoire, Rhonda (MAFRI) Sent: Tuesday, November 20, 2012 5:32 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Processors Does anyone have the Thermo Shandon Excelsior or the Leica ASP300 S tissue processors? What do you like or not like about the one you have? Thanks Rhonda Gregoire, R.T. Charge Technologist Clinical Pathology/TSE Section Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Initiatives 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email rhonda.grego...@gov.mb.camailto:rhonda.grego...@gov.mb.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ENFD Testing
Happy Wednesday Histonetters I have had a request from some of our clients to do epidermal nerve fiber density testing. I have found information in how to obtain the specimen but have not found the protocol for processing, cutting, and staining the specimen. To anyone out there who is performing this testing, I would appreciate your insight. I am starting this from scratch. Any information would be welcome. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Eosin staining for small biopsies
We also use safranin on all our small biopsies and also our breast biopsies with no adverse effects to any of our IHC or ISH. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Wednesday, June 20, 2012 10:32 AM To: histonet@lists.utsouthwestern.edu; Carol Freeman Subject: Re: [Histonet] Eosin staining for small biopsies We use safranin (used in Microbiology as a counterstain) on our small biopsies. We apply a small drop during grossing. It does not affect staining of HE, IHC or ISH. We like this because it is an intense red that doesn't leach out in the alcohols of processor. Cindi Robinson HT(ASCP) Mercy Medical Center Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 phone-712-279-2768 robin...@mercyhealth.com Freeman, Carol carol.free...@utoledo.edu 6/20/2012 8:46 AM Good Morning all, Happy Wednesday! I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol?? I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result?? One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree? Thanks for any responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene Substitute for Counterstain Clearing
Andrew You could use Clearium from Leica. Clearium can either be coverslipped from xylene or isopropyl alcohol. Drying time from isopropyl is longer then xylene. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew Coleman Sent: Friday, May 11, 2012 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute for Counterstain Clearing Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene microspheres. The spheres are inert to alcohol, but are washed out when we clear with xylene to coverslip. The spheres are also supposedly soluble in DMF, acetone, acetonitrile, chloroform and methylene chloride for what its worth. Is it reasonable to coverslip these slides in permanent mount without clearing with xylene after dehydrating the tissue? Or does anyone know of a substitute clearing agent with chemical properties dissimilar enough from xylene that might be worth trying instead? Thanks, Andrew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] vaginitis panel
I know. I was hoping to see what everyone else is doing. -Original Message- From: Kim Donadio [mailto:one_angel_sec...@yahoo.com] Sent: Friday, May 04, 2012 6:30 PM To: Cynthia Pyse Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] vaginitis panel It's Friday :) Noticed none touched this? Sent from my iPhone On May 3, 2012, at 4:11 PM, Cynthia Pyse cp...@x-celllab.com wrote: Histonetters For those of you performing vaginitis panels, how are you billing these? Do you bill for each separate test or use the combine CPT code? We are having a discussion on what is our best method of billing. Thanks in advance for the info. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] vaginitis panel
Histonetters For those of you performing vaginitis panels, how are you billing these? Do you bill for each separate test or use the combine CPT code? We are having a discussion on what is our best method of billing. Thanks in advance for the info. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] DeCloaking Chamber
Karen I would just purchase a timer to plug the Decloaking chamber into, I use one to heat my water bath for pretreatments prior to by techs coming in. I purchased the timers from Fisher, cat. #666224 for $28.82. Works like a charm. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, May 02, 2012 8:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DeCloaking Chamber Hi, Does anyone know of a delayed start Decloaking chamber that is independent from the IHC stainer? I am looking around to get a new Decloaking chamber. Any suggetions? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Batch Controls
What do you do if the IHC slides need to be sent to another facility. Currently we place a control on every slide we run. I know this is using a lot of tissue but I sometimes have 3 different cases going to 3 different facilities for patient treatment. Also we stain all the IHC stains for 5 different hospitals. I can't see any way of getting around putting the positive controls on the slides. With having 14 different Pathologists this seems to work out best. How is everyone handling this? Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, April 18, 2012 3:41 PM To: Amy Self; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Batch Controls As the laboratory Director, I can sign-off on the positive control slides or my staff can do it since I have delegated this responsibility to them. We all use a different color pen to mark the slide(s) O.K. so we always know who signed-off on the control slide. All positive controls are filed by date (the date is printed on the label). I believe that we can back-track (using the staining logs) to identify which machine the control was run on (if needed). Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Amy Self as...@georgetownhospitalsystem.org 4/18/2012 9:55 AM Good Morning and Happy Wednesday to everyone, For those of you that are running batch controls with your IHC stains I have a couple of questions for you. What is your process for review and documentation of the batch control slide? How do you label/identify this slide for easy retrieval? (Do you identify this slide by date run or stain run) Thanks in advance for everyone's help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] know error system - prostate biopsies
We had one of our clients looking into this testing. The company does a hard sell. What they neglect to tell you is the insurance companies may not cover this testing leaving the patient with a huge bill sometimes $700 to $800. Known Error will tell you it is a Medicare approved test what they don't tell you is the patient will almost always end up with some bill, also the other HMO do not have approval leaving the patient with the full bill. Our client declined to use this system after further investigation form our lab. The amount of work evolved in processing and hands on specimen time is huge with no reimbursement to the lab itself. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Friday, April 13, 2012 9:25 AM To: Richard Cartun; Histonet Subject: RE: [Histonet] know error system - prostate biopsies We do not! Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, April 13, 2012 8:21 AM To: Histonet Subject: [Histonet] know error system - prostate biopsies I am curious how many of you working in hospital or non-hospital Anatomic Pathology labs are using the know error system for prostate biopsy/patient DNA confirmation? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block Storage-another question
If the pod lab is in NJ and the reading lab is in NY, which guide lines do you follow. NYS requires us to save our blocks for 20 years. Due to storage issues, I would rather the pod lab store the blocks. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 21, 2012 10:20 AM To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails Subject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. René J. --- On Wed, 3/21/12, Nails, Felton flna...@texaschildrens.org wrote: From: Nails, Felton flna...@texaschildrens.org Subject: RE: [Histonet] Block Storage To: 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional componentor should they have the facility performing the professional component store them? Who is ultimately responsible? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cytokeratin AE1/AE3
Joanne What Dako platform are you using, link or 48? On the link I pretreat with PK, the IS series antibody is used with an incubation time of 10 minutes.. On the 48 I pretreat with High pH TRS, the IR antibody is used with an incubation time of 20 minutes. Both systems give me clean staining.(I'm in the process of phasing out the link.) I would try either decreasing the incubation time of the antibody if you stay with the HIER pretreatment or try the enzyme pretreatment with your current protocol. Good luck. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Clark Sent: Tuesday, February 28, 2012 11:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytokeratin AE1/AE3 Hi Histonetters, I am having some issues with my Cytokeratin AE1/AE3. We use the antibody from DAKO and do HIER on it before staining. We run AE1/AE3 as a protocol on sentinel lymph nodes from breast cases that were negative on frozen section to rule out micro metastases and what my pathologists sometimes sees is staining that looks like it's running in-between the cells. It is not an indication of metastases but my pathologists want me to get rid of it. Have any of you seen anything like this? I would especially appreciate the opinion of Samuri Pathologist on this if it isn't too much trouble. Thanks all! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico Roswell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] egfr
Good Morning Histonetters I am looking to add EGFR to our antibody list. What clone is everyone using out there in Histoland? Any info would be greatly appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Humidity levels and IHC staining
NY in winter has low humidity, I still have no problems with my Dako stainer. Make sure the lid is closed. I had a tech who consistently left the lid open, there was some drying on the first row of slides. Closed the lid the problem stopped. Are you using Dako buffer? The surfactant in the buffer should prevent drying. You might want to run it by the Dako tech service. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 17, 2012 2:27 PM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Jason McGough Subject: RE: [Histonet] Humidity levels and IHC staining Renee, it can depend on where you are: Florida? 70, 80% humidity, no drying out. South Dakota, in winter? 10 percent humidity and you get drying problems. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 17, 2012 11:20 AM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? René J. --- On Fri, 2/17/12, Jason McGough jmcgo...@clinlab.com wrote: From: Jason McGough jmcgo...@clinlab.com Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] question
Good Morning Histonetters One of our clients is interested in starting to compare biopsy tissue with a DNA swab. The test name is Known Error test and it is performed at a company in Utah. Does anyone out there know what this testing entails. I could use any information since I have no knowledge of this test. Thanks in advance for your help Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bleaching melanin
Hello Histonetters I need to bleach melanin out of some paraffin sections. The only bleaching solution I have is a 3% H2O2. I know you can bleach melanin in a 10% H2O2 solution for 1 to 2 days. How long do you think it will take to bleach the section in a 3% solution? Of course everyone wants it yesterday or I could order the K permanganate and oxalic acid I really need. Any suggestions would be greatly appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] immuno stainers
Hello Histonetters Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how times flies when you have time off. Now it's back to the old laboratory grind. We are in the market for a new immuno stainer. I currently use a Dako Link, which I am very pleased with. I have demoed the new Dako with great results, unfortunately we need 2 stainers and the pricing maybe a little out of our range. Is anyone using the Biocare Intellipath? What are the pro's and con's? How is their service? I currently use their detection system, Mach 4, but the majority of my antibodies are purchased from Dako. Any input on the daily use of the Intellipath from current users would be appreciated. Thanks in advance for the input Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HPV high low risk
Hi Histonetters I am looking to add to our test menu the High and low risk HPV. What is everyone using out there. Instrumentation? Vendor? Pros and cons to any system. I would appreciate any input. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Automated Slide Stainer and Coverslipper: Foil or Glass?
Martin We currently have a Leica CV35030, which is a glass cover slipper. My 15 Pathologists prefer the glass covers slips for the optical quality. We are required to archive our slides for 20 years, which is another reason we chose glass cover slips. The Leica CV5030 if cleaned nightly and maintained properly is a work horse, with only small problems that are easily fixed within the lab. Cindy Cindy Pyse, CLT, HT(ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hasselblatt, Martin Sent: Tuesday, November 08, 2011 4:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Slide Stainer and Coverslipper: Foil or Glass? Dear list members, we have funding to buy an automated slide stainer and coverslipper and are considering to go for the Tissue-Tek® Prisma. If you had the choice: Would you opt for a glass coverslipper or a foil coverslipper? Feedback of pathologists on the optical quality of the foiled slides as well your technical experiences with maintenance (we heard rumors the glass version is running less stable) are most welcome. Please take into account that we plan to archive slides infinitely and will not discard them after 5 years. Thank you very much for your help! All the best from Germany, Martin Martin Hasselblatt, M.D. Professor of Neuropathology Institute of Neuropathology Universitätsklinikum Münster 48149 Münster, Germany ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Specimen Tracking for courier
I would be interested in everyone responses. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bull, Jennifer L. Sent: Thursday, November 03, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Tracking for courier I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] unstained slides
Hello Histonetters, Recently we have seen an increase number of unstained slides requested from outside facilities. We do not release our blocks, so unstained slides are the only option for any facility to obtain our tissue. Currently we do not charge for these slides, but I am rethinking that policy. What is everyone doing about unstained slide requests? Are you charging for the slides? How much per slide? Any information would be helpful. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Old Green Staining Racks
Market Lab cat#ML1212. I would check the cat# before ordering, the picture in the catalog show the right rack but the description is different. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Tuesday, August 09, 2011 11:39 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Old Green Staining Racks Does anyone remember the old green staining racks that had an inverted L metal handle and held 25 slides? We are trying to find somewhere to order them, but having no luck. Any suggestions are welcome! And, thanks in advance! The Histochicks at Sharon Regional Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cam 5.2
Thanks to everyone for the Cam5.2 info. A great weekend to all. Cindy Cindy Pyse, CLT, HT (ASCP) Laobratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cam 5.2
Hello Histonetters What company is everyone buying their Cam 5.2 antibody from? Thanks for the information. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] alcohol as a fixative
Most clinician I know have other staff in the procedure room. Why not have someone other than the clinician place the specimen in the formalin. This way you get the best possible fixation for the patient. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tryka, A. Francine Sent: Wednesday, July 13, 2011 11:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alcohol as a fixative Hello all in histoland. I have a clinician who may be sensitive to formalin. One of my colleagues suggests that he place his endoscopy specimens into alcohol instead of the usual formalin to mitigate his exposure. Have any of you dealt with this problem? What percent alcohol would you suggest? Or methanol? Would you transfer it back into formalin upon receipt in the lab? Thanks, Franci Tryka Correspondence, including e-mail and other electronic communications, to and from employees and elected officials of the Teton County Hospital District, dba St. John's Medical Center, may be subject to disclosure under the Wyoming Public Records Act. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is strictly prohibited. If you are not the intended recipient, please notify the sender by reply e-mail and destroy all copies of the original message. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] water
Hi Histonetters I have 2 tech off on vacation so I thought I would take the easy way this time. Does anyone know the difference between distilled water system and the reverse osmosis water system? Could if effect the IHC staining by using one or the other? Usually I would research it myself but it has be crazy around here lately. Thanks in advance for any help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 Ext.232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Retirement
As long as we don't have to go back to steel knives, old AO micrtomes and the autotechnicon I'm in. I am also 15 to 18 years out. What is the world of histology going to do without us. Who will know how to make a solution of mucicarmine (not that I do anymore, but I could) or eosin? The tech I train now look at me like I am speaking a foreign language sometimes. Make me feel old, but closer to retirement. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, June 17, 2011 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retirement OK, I know it is Friday, and I know that this may sound like a bit of a jokebut I am 15-18 years out from retirement and my wife and I want to retire someplace tropical And it would be smart to get settled in such a location. So, if anyone knows of any openings in Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT (ASCP) QIHC PLEASE let me know. Would be open to others, but would prefer a US territory. I can be reached at b...@deaconbill.com William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist/Safety Officer Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] validation sheet
Happy Friday Histonetters Does anyone have a validation log sheet they have used for the validation of a recycler. Someone spilled xylene on our copy then proceeded to discard it without coping the sheet first. I would appreciate it if anyone would share the log sheet they have used, it would save me some time. Thanks in advance. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decal
Hello Histonetters What is everyone using to decal specimens. I haven't had to use it in a few years, before that I just purchased formic acid and made a 10% solution. Just wanted to see if the criteria has changes over the years. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Bond III
Hi Histonetters I am testing the Leica Bond III immunostainer. How is everyone handling processing the Hercept test from Dako on the Bond III? I cannot use either the approved pretreatment or visualization system, not to mention the approved buffer. I currently run my immunos on the Dako so it has not been a problem. I would appreciate any input anyone has. Thanks in advance. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cyto prep
Good Morning Histonetters For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Immuno's
Do you use a hydrophobic pen to circle the tissue. This concentrates the solution where you need it. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 etx.232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, April 11, 2011 10:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno's Hi Folks.. Looking for a bit of help. Is there anyone out there who are doing their immuno's manually? Our Pathologist has just informed us that our immuno's are ALL too weak. He states that they always have been, as he compares them to slides that we get back from the reference labs that we use for the antibodies that we don't do in-house. If you are doing them manually...what companies are you using for antibodies, detection kit and chromogen? And are your stains really dark? I informed our Pathologist that I know for a fact that the reference labs that we use, do the immuno's on stainers, and naturally these stains will be consistently be stronger. I am trying to see if I can keep costs down by trying not to have to buy a stainer and continue to do the immuno's manually. If you all know of a wet-workshop or of a immuno. company who would send someone to our facility to teach us ( the latter is preferred, since my section chief is going to retire in 1 1/2 weeks, I will be taking over her duties and this is going to leave us with only myself and one other tech, until we can get a replacement for my position). Any help or advice that can be given will be greatly appreciated. Thanks so much. Valerie Hannen, MLT(ASCP),HTL,SU (FL) Parrish Medical Center Titusville,Florida ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] how to bleach melanoma cases without bad effect on IHC
Sabeti I use the AEC chromogen (Dako catalog #K346989) for my melanoma cases. I use a aqueous mounting media, placed on the tissue only, dry it completely on a hot plate. When dry I coverslip it with permanent mounting media(don't place it in xylene), dry coverslip. Works great, and the color doesn't fade. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shahram Sabeti Sent: Tuesday, March 22, 2011 4:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to bleach melanoma cases without bad effect on IHC hello dear fellows, i have an IHC project on paraffin embedded melanoma cases; some of them being heavily pigmented. i have seen many protocols based on H2O2 to bleach the pigmented ones, but i am not sure about any possible effect on antigen retrieval.please guide me . yours, sabeti ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HercepTest
Cindy I use a Fisher Isotemp 205 waterbath. The end result is beautiful. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cindy Bulmer Sent: Tuesday, March 22, 2011 1:12 PM To: Histonet Subject: [Histonet] HercepTest I understand the HercepTest calls for a waterbath for the epitope retrieval process... but, I'm wanting to know what type of equipment, ie: waterbath make and model or if people are using the Dako PT link module. Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histotech is suppose to cut per hour?
Practice, practice, practice! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine he slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] procedural safeguards when accessioning identical sources
Carol We mark all our prostates with dye for ease when embedding. For prostates that need to be accessioned in order we alternate the color of the dyes, hematoxylin, safranin, eosin. When grossing, the color of the dye used is in the dictation. Hope this helps. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, February 24, 2011 3:48 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] procedural safeguards when accessioning identical sources Our lab has the policy of not accessioning back to back specimens of the same source. This helps us to ensure if there is a mix up the pathologist can tell when reading the case. For example if the specimen source is a skin and they have an endocervical they would know. We are soon to be getting a higher volume of prostates and will have to accession them together. What kind of procedural safeguards do you have in place when working with a high volume of identical sources? Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks
Happy Friday Histonetters I want to thank everyone for the information on IHC strainers. This is just what I needed. People who actually use the machines on a daily basis giving their opinions. What a great forum we have. Hope everyone has a great weekend. Currently we are receiving 6-10 inches of snow, hey, what do you except in Buffalo. Thanks again. Cindy Cindy Pyse, CLT, HT(ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Equipment
Hi Histonetters I currently use a Dako stainer for my IHC staining. It is a work horse with very little problems. It is a older model that we may need to replace in the near future. What is everyone using out in histoland. I would be perfectly willing to purchase another Dako but I want to explore all avenues before making a decision. What are the pros and cons of the instruments any of you are using. How often is the machine down? What is the capacity? We run the Dako twice daily usually to the capacity of 48 slides. I would like to hear only from actual user of the instrumentation, no vendors please. This is only a fact finding e-mail. Thanks in advance for all your input. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Viper Affrim
Hello Histonetters We have recently added the BD Viper and Affrim testing to our lab. Training is underway. I would like input from the experts out there on how much time is actually spent on setting up both the Viper and Affrim testing. I know what the manufacturer says I just want the opinion of someone who is actually performing the testing. I need to consider the scheduling changes I need to make. Thanks in advance for any help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] gloves
Happy Monday Histonetters I am looking for a reasonably priced, non-latex glove that will hold up to xylene. The purple knight from Kimberly-Clark works great but is cost prohibited. My general manager want me to find a cheaper alternative. I have had several samples but none will hold up to any exposure to xylene. We were previously using the black panther gloves which were working well, but according to our supplier are no longer available. Any advice from you vast experience would be appreciated. Thanks in advance. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: DAB
From: Cynthia Pyse [mailto:cp...@x-celllab.com] Sent: Tuesday, September 28, 2010 3:48 PM To: 'histonet-requ...@lists.utsouthwestern.edu' Subject: DAB Hello Histonetters I need some expert advice concerning the disposal of DAB. Our lab is in NYS and there has been some discussion on the proper way to dispose of DAB. I've done the research, and found household bleach is no longer acceptable. There is the potassium permanganate treatment, the horseradish peroxidase treatment, and also disposing of it the regulated medical waste that is incinerated. What is everyone using? Does NYS have different standards than other states or is this a federal regulation? If you dispose of the solution is the RMW, how do you protect the techs when pouring the waste DAB from our IHC waste container into bottles for disposal? If there is a spill can it be cleaned up within the lab or does a Hazmat team need to be called. I want to make sure we cover all our bases. I don't want any problems with NYS. Thank you in advance for all your input. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura VIP6
Help! I am having a problem with me VIP6 tissue processor. About every 4 to 6 weeks the processor has a error code that position 2 which is 80% has not pumped in fully in the allotted time. Sakura answers to this problem is either I need to use their formalin or the alcohol in the second station is too high a concentration. I have never had this problem before in any processor I have used. I still use the Shandon excelsiour tissue processor and use the same protocol in both machines with no problems in the Shandon. Sakura thinks it is a build-up of precipitating salts that is causing the problem but there was no sign of this when the service tech checked the rotary valve. After every process we not only flush with ETOH and xylene but also run a warm water flush from the first station which is formalin. I use a different bottle for the warm water but do use the same valve from the formalin bottle. When we have completed 7 runs we run the 3 warm water flush Sakura recommends. Even the service tech remarked that I was doing more than they recommend. I am at a loss as to what it could be. My only conclusion is that maybe the valve in station 2 intermittently collapse or sticks for some reason. I wanted to see if anyone either is having the same problem or if someone has a thought of what the problem could be. I did purchase a refurbished machine. I would appreciate any thoughts, ideas, or even guesses that anyone has. Thanks in advance for any help. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura VIP6 update
Thanks everyone for your responses. Sakura has scheduled an upgrade of my rotary and gate valve. I hope this is the answer I need. Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water collecting at bottom of sections
I second Linda's technique, the only other option I use is to bring the slide up at a negative angle so the slide comes out of the water bath first then the section. I find this works well for us. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, July 23, 2010 2:42 PM To: nap...@mail.siscom.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Water collecting at bottom of sections I routinely flick my wrist holding the slide with the section I've just picked up. Usually, this is enough force to release any water at the bottom of the section. If that doesn't work I melt a tiny hole at the bottom of the section with a heated probe and flick again. I'm sure the manufacturer is right in saying that its the coating; the paraffin adheres too well and too quickly to the slide trapping water underneath. It is also very important to hold your slides as vertically as possible when bringing them under your section and raising the slide out of the water bath. That way you will trap as little water as possible underneath. Hope this helps. From one long-time-microtomist to another, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of nap...@mail.siscom.net Sent: Friday, July 23, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water collecting at bottom of sections Hello all, From time to time and depending on what brand of adhesive (or charged) slides I am using, I seem to get a bag of water that drains to the bottom of my sections but doesn't drain out. I have been working in microtomy a long time and have had to deal with this contingency time and time again, but never really have gotten to the bottom of the problem. I spoke with a premium manufacturer of such slides and they seemed to indicate that it is a problem with the coating, but couldn't tell me for sure. All I know is that certain brands do this more than others. If you know what I mean, you know it is a problem. My bath is pure distilled H2O with no gelatin or Sta-on added. It is if the adhesive properties are SO good that they will not release the water when vertically drained and have to be shaken off or cut with a razor blade at bottom to release the water. Anyway, if anyone has an insight or two on this, I would be interested. It seem sthe most challenging issues are ones that seem related to some of the most simple tasks that one has performed for many years!! Manufacturers understand what I mean, but cannot pinpoint the problem for me via phone or e-mail. Anyone see this and have a chemical/mechanical solution they have developed over the years? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biocare P63 with Dako Flex detection
Cathy Have you tried adding an EnVision FLEX mouse linker to your protocol. Sometimes that will work. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cathy.crump...@tuality.org Sent: Wednesday, July 21, 2010 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare P63 with Dako Flex detection Hi all, I have ran into a troubleshooting dilemma and was wonderi=g if anyone else could help. Is there anyone out there that uses the Biocare Medical P63 concentrated antibody with the Dako TRS and Flex detec=tion kit? I am curious what dilution you use and if you have to do an=ything special for the P63 (extra long retrieval, DAB enhancer, etc). = am having problems getting a strong signal for the basal cells in prosta=e. I am running it at 1:50 with 40 minutes in pH 9 retrieval (which =seems too long to me but does help). A dilution with the same protoco= at 1:100 is way too light. Thanks, =DIV Cathy Crumpton HT(ASCP), Histology Lead Tuality Community Hosp=tal Hillsboro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] inking dyes
I use tattoo dyes. They work great and are much cheaper than tissue marking dyes. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Thursday, July 15, 2010 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inking dyes Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] BILLING QUESTION
We have a contract with the hospitals, this comes from our billing manger. We bill the hospitals, then they bill the patient. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Friday, July 02, 2010 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BILLING QUESTION Histonetters: If you do some work for another hospital (Histology) can you bill the hospital or do you have to bill the patient directly? Is there a statute or Regulation out there about this? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] water bath information
I am also looking for the same type of waterbaths and would appreciate any information. Thanks Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 08, 2010 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] water bath information Hi Guys- We need new waterbaths and I'm looking for a specific type. It's blue with a square glass baking dish inset on the top. We don't care if they're blue but the small footprint with the glass insert is the key-- Vendors and refurb dealer responses welcome- Thanks! Cheryl Kerry, HT(ASCP) Houston ker...@labcorp.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Is anyone else having an issue with their paraplast?
I'm relieved that someone else is having the same problem. I was beginning to think it was my processor. I have already decided to switch paraffin. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hayes, Sherry Sent: Thursday, May 13, 2010 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Is anyone else having an issue with their paraplast? We are having an issue with our paraplast (manufacturer McCormick). There are black flecks and what looks like a dark sand in the paraplast. The issue has continued for @8 weeks and has happened with several lot numbers. Is anyone else experiencing this issue? Sherry S. Hayes Laboratory Systems Support Coordinator Bloomington Hospital 601 West Second Street PO Box 1149 Bloomington, IN 47402 t812.353.5606 f812.353.5584 p812.334.6337 sha...@bloomingtonhospital.org mailto:sha...@bloomhealth.org bloomingtonhospital.orghttp://www.bloomingtonhospital.org/ CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If you are not the intended recipient, you may NOT use, disclose, copy or disseminate this information. Please contact the sender by reply e-mail immediately and destroy all copies of the original message including all attachments. Your cooperation is greatly appreciated. Bloomington Hospital P.O. Box 1149 Bloomington, IN 47402 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gastric biopsies
Christi We run a diff stain on all gastric bx suspected of H. pylori as routine, it saves us time. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Wednesday, May 12, 2010 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gastric biopsies Hello Histoland, I was wondering if anyone out there does stains other than HE 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD68
Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks
Thanks to all for providing me with the information I requested. You have made my decision making so much easier. Thanks again Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC on FNA
Happy Friday Histonetters What is everyone using for controls for FNA IHC stains. We routinely stain human FFPE sections and only have controls for tissue. Recently we had a few cases when the only sample was an FNA to perform IHC on. Any suggestions would be welcome . Regards Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC on FNA
Linda I agree with you that is why I asked the question. We do not have a cryostat, so frozen sections are out of the question. I thought of making cytospin slides out of positive fluid but then you run into the problem of any positive cells making it onto the slide. Would any of you run the FNA slide without pretreatment and the FFPE control with the pretreatment? I appreciate everyone suggestions. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: Sebree Linda A [mailto:lseb...@uwhealth.org] Sent: Friday, April 09, 2010 12:40 PM To: Cynthia Pyse; Histonet Subject: RE: [Histonet] IHC on FNA This topic touches a nerve with me. We recently went back to receiving requests on FNAs after years of not doing any. When we were routinely staining them with IHC, we would use a frozen tissue section for a control as the same protocols generally worked for both, i.e. no HIER, protease, etc as may be needed for FFPE. When we stopped doing FNA IHC routinely, we eliminated our frozen section control inventory. Now that we're doing them again, it was decided to use FFPE controls and their accompanying IHC protocols. I do not agree with this practice as I feel there are too many differences between FNA preparations and FFPE sections. I was voted down so now we are using HIER, protease, etc. whatever the FFPE protocol calls for on these FNA preparations. Surprisingly, at least to me, there are still cells left on the FNA preps after even the harshest retrieval protocols. We also don't run negative controls of the FNAs. The argument being that there are totally different cells from one slide to the next so a comparison between a negative control slide and one that received antibody is useless. So that's what we're doing even though I don't agree with it. At the time this all came about, I also queried Histonet as to common practices so the archives should have some info. also. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 09, 2010 11:16 AM To: 'Histonet' Subject: [Histonet] IHC on FNA Happy Friday Histonetters What is everyone using for controls for FNA IHC stains. We routinely stain human FFPE sections and only have controls for tissue. Recently we had a few cases when the only sample was an FNA to perform IHC on. Any suggestions would be welcome . Regards Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] histo techs doing cyto prep work
That is how I started many moons ago(29 years and counting). I spent 1 week in histology, the next week in cytology, this continued for 2 years. I currently supervise both the histology and the cytology lab sections. My histotechs do little cytology prep work but are willing if time allows to help out at the end of the day. The cyto prep techs are willing to learn histology but there is little time to take advantage of their willingness. Hope this answers your question. Happy Friday Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Friday, March 26, 2010 12:43 PM To: Histonet Subject: [Histonet] histo techs doing cyto prep work Hi all, I'm curious!! How many of you histotechs are being trained or already know how to do cyto prep work? And how many of you histo supervisors are now supervising cytology labs in addition to the histology lab? Is cross training histotechs to be cyto prep techs (in addition to their histo job) becoming more popular? I welcome any and all responses... ~Kim Tournear ~ HT (ASCP), QIHC (ASCP) Histology Supervisor Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] coverslipper
Leica CV3050 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, March 25, 2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslipper Hi Colleges, I need your opinion about coverslip by hand vs. using machine. If you use machine what company's coverslipper you prefer? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mc Coy fixative
Hi Histonetters I am having a senior moment. Can anyone tell me what McCoy fixative is made of and what it is used for? Thanks in advance for the help. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Removing Yellow color from slides refixed in Bouin's solution
I just place the slides into 80% ETOH for 2 minutes. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cscam...@uci.edu Sent: Monday, March 15, 2010 7:42 PM To: HistoNet Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's solution Hi Histonet, After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it very difficult to remove the yellow coloring. Rinses with water are not doing the trick. Does anyone have some advice on how to bring the slides back to a clear color so that I may proceed with the Masson Trichrome procedure? Thanks! -Colin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Excelsior Vs. VIP 6
Matt We have both the VIP 6(new)and the Excelsior(older). The Excelsior is a little less user friendly but is a workhorse if maintained properly. There is less solution exposure since you do not have to fill the containers as you do in the VIP6. Both machines produce excellent tissue blocks. The VIP6 has many safe guards so there is little chance for mistakes. With every step in the VIP6 it asks for verification of the command. If you have any more question I can answer feel free to contact me. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com 716-250-9235 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Thursday, March 11, 2010 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Excelsior Vs. VIP 6 Hello to the World Wide Histo's, We are looking at getting a new processor and was wondering what everyones feelings were on the Excelsior from Thermo vs the VIP 6 from Sakura. We have a VIP5 right now and it is a delight. The demo of the Excelsior was very impressive. Thanks for your thoughts. Matt HT (ASCP) Longmont United Hospital Longmont, Colorado ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16
We purchase our p16 through MTM laboratories reference # 9517. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Tuesday, March 09, 2010 5:28 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] p16 Hello fellow Histonetters, Does anyone run the antibody p16? If so, where do you purchase it from and maybe you can share your protocol with me? Thank you in advance. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center (701)-530-6730 dknut...@primecare.orgmailto:dknut...@primecare.org This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Helicobacter Pylori antibody
We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20 minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare) 10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes hematoxylin(Dako). Works great. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain Sent: Tuesday, March 09, 2010 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] fontana
Fontana-Masson also stains for Argentaffin granuals. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Wednesday, February 24, 2010 9:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fontana Does anyone know of anything other than melanin that Fontana Masson would stain? Also, does anyone know of a stain that would label un/saturated fatty acids? Not sure if this is even possible. Thanks in advance. Kris Kalleberg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tape/Film Coverslips vs. Glass
Cathy I prefer the glass coverslips. It seems to give more protection to the tissue. Recoverslipping is also easier than with the tape. Just my opinion. Hope this helps. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cathy.crump...@tuality.org Sent: Monday, February 01, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tape/Film Coverslips vs. Glass Hello all, We mig=t be getting a new coverslipper and the pathologists asked me to get feedb=ck from my fellow histotechs that have used both glass and tape coverslips=. Which type is most prefered for general histology and cytology?nbs=p; I did some research in the archives and most of the responses were dated=004. Has anything changed about the tape systems since then? =I would be interested in receiving your input. Thanks, Cath= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Sakura iDent
Anne You might want to try going up the Sakura chain of command. I had trouble with a coverslipper, after contacting the regional manager it was amazing the service it received. Good luck. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Wednesday, January 13, 2010 4:16 AM To: Denise Van Eaton Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Sakura iDent Hi Denise sadly no resolution also sadly very little response from the histonet - except for Rene - he and i did some troubleshooting to try to see if i had missed anything - we had the same thoughts so ... i am still sitting with the iDent in its box, unused, until someone, somewhere can convince me that the print does not wipe off with xylene - which will be hard to do seeing that i wiped it off myself and saw with my own eyes that xylene removes the ink!! Sakura - if you are reading this - i am not a happy camper Annie 2010/1/12 Denise Van Eaton dvanea...@littonlab.com Hi Anne, We have been checking into cassette printers. Obviously, if we can bring one in for a demonstration we will but I thought the iDent looked like a good place to start. I noticed your problem (on the Histonet) with the ink rubbing off after the xylene. I never saw any responses from the rest of the Histonetters... did you resolve the problem? Assuming you (or Sakura) did, what was the trick? Thank you in advance for sharing your fix for a potential problem. Denise Van Eaton HT(ASCP) Litton Pathology Associates -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bachelor's Degrees
Tom, I agree with you about Nate qualifications. Unfortunately in NYS if you bring in outside work your tech MUST be licensed in NY. The copy of the license need to be displayed in the lab. I haven't found any way to hire employees without a license. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Saturday, December 05, 2009 3:23 PM To: Nathan Jentsch Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bachelor's Degrees One more reason to consider carefully before throwing support to state licensure where it does not exist. I feel sorry for you Nathan and I'd like to have someone explain the upside of licensing to you. It seems it's not about having a license (like a driver's license) to practice histology. I fear it's just more about fattening state coffers and adding another level of bureauracracy to things. If you are educated (as you are Nate) and if you are academically eligible to sit for the registry exam. And if you can satisfactorily pass the exam, what has state licensing got to do with it? Are you a better histologist in New York because you're licensed as opposed to your neighbors in PA, for example who aren't? I think not. Does licensing prove something that science degrees and registry certifications do not? Maybe I just don't get it. And I'm not trying to pick a fight here with the supporters of licensing. I just haven't heard a good convincing argument for it yet. I'm also quite certain that even though monetary compensation has improved somewhat, the last thing most Histologists need is another payment. The privilege to work in a certain state, which is paid for (by you) nothing more?! I suppose some kindly employers out there somewhere could pay for it...good luck with that. Here's an idea, let's say you're degreed and registry eligible and/or have passed your board exam(s) and are certified. How 'bout the state says you've met the qualification for licensing, here you go! Nate you are degreed and certified and in my book and in the book of the current state I live in - Oregon - and the states I've worked in - Wisconsin, Michigan and Minnesota - you are more than qualified to work. I for one would not hesitate in the least to consider a person such as yourself for employment. Again you are more than qualified, even though you are unlicensed. I guess I just don't understand how credentialing - degrees and certifications - aren't enough, but licensing is the magic ticket to better science/medicine/patient care/whatever. I'm sure some folks out there will bring on the firestorm, but again Nathan I feel sorry for you and I don't see the reasoning behind this. Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nathan Jentsch Sent: Saturday, December 05, 2009 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bachelor's Degrees Paula, Let me tell you that this is an extremely frustrating point for me not for getting a job but for getting a license in New York State (which is related because I'm technically supposed to have a license to work). Despite the fact that I have a B.S. in a science field and have been working competently at my job for almost two years now, the state wants me to have an A.S. in histotechnology to get my license. They won't even consider HT certification as sufficient. If a collective group of experts in the fields of laboratory science and pathology say I'm qualified, why isn't that good enough for a bunch of beurocrats who can't even manage the pocket book of our state. Nate ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
