RE: [Histonet] Block Counts
I have always found there are so many variables that having an expectation of setting a set number is not always possible. I feel they should be compared to their own standards and not of their co-workers. If they can cut or embed a set number on a particular than they should maintain or gradually increase (for newer techs) over time. . I have seen where in one day they cut so many blocks when the work load mandates it but on a slower day it takes them just as much time to cut half as many blocks. The work pace should be at their comfort level but should be a standard rate. Diana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: January-12-15 4:58 PM To: Ellen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block Counts Depends on the type of tissue. Depends on the length of time your day is for a repetitive task. Assigning an arbitrary number is counterproductive. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen Sent: Monday, January 12, 2015 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Counts I'm looking for raw data on time studies that directly deal with the number of blocks a PA can produce in a day and how many a histo tech can cut a day. Thanks Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Aspergillus tissue blocks for controls
As well we have used mushroom and other moldy food products but it only demonstrates the mold and not tissue elements. But you can mince tissue and mix it with the fungus or mold so it one big segment and this gave more desirable results. Diana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder Sent: November-04-13 4:47 PM To: Patrick Laurie Cc: histonet@lists.utsouthwestern.edu; Vickroy, Jim Subject: Re: [Histonet] Aspergillus tissue blocks for controls We have tried the moldy peel also but the pathologists did not like this and would not accept any controls of this form. I don't blame them they want to see it naturally occurring. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie foreig...@gmail.com wrote: Hi Jim, I have found a couple of ways. First, if you are fortunate to have a micro department nearby, they can make a very nice one for you (a Histotip in Sakura's Histologic). Another method, which doesn't work the best, can be moldy orange peel. And the easiest, you can create your own (moldy sausage) or even take some human tissue (fresh lung works the best) and leave it with some moldy meat, then you can get a great one. Good luck! On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim vickroy@mhsil.com wrote: We are in desperate needs of obtaining GMS controls. In the old days we had all the fungal specimens we needed. Today it is getting hard to find these tissues or controls. Does anyone have any idea where we can get tissue blocks with Aspergillus or does anyone have any other suggestions about GMS controls. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Endometrial biopsies
In the past when we receive these samples there has been no issues cutting them. Over the past few months there has been a tendency for these blocks to shred into fine strips when cut. Almost like there was sand in the blocks. Mollifex, decal, warm or cold, doesn't matter they still shred. Getting levels is such a challenge. I have inquired at the collection site to determine if there is a change in the process of collecting that could cause this. I have ensured the samples are not left on gauze or something to allow them to dry out. Even blocks with lots of blood and/or mucous will shred as well as blocks with only a scanty amount present. Is anyone else experiencing this. Diana Chatham Kent Health Alliance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PowerPath QA
Is anyone using this module and are you willing to share any documents regarding it. Or opinions on what think of it for a QA program Sincere thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryomolds without a cryobar
There was a recent post for someone inquiring about cryomolds. I have not used these and have researched them and found out the Sakura cryostat has a cryobar with recessed ports to accommodate these molds. Has anyone used these on a Microm microtome. Is there a problem removing the molds and having them separate from the chucks? diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking for cryomolds
I did not know such a product existed until now. How do these molds work? Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of margaret chadwell Sent: July-29-13 12:24 PM To: histonet@lists.utsouthwestern.edu; Carol Torrence Subject: Re: [Histonet] Looking for cryomolds Hi Carol PSL (Pacific Southwest Lab Supplies) sells the round Cryomolds. Margie Chadwell Sent from Windows Mail From: Carol Torrence Sent: Monday, July 29, 2013 9:18 AM To: histonet@lists.utsouthwestern.edu I found an old box of Tissue Tek 4728 cryomolds in our Mohs lab. They are round with a small tab on the side. We like them so much better than the disposable embedding molds sold for paraffin embedding. I have seen cryomolds that look like the same ones for paraffin but are they any thinner for quicker freezing? 1. Does anyone know where to get the round ones?I do not see them on the Sakura website. 2. Do the square ones that look like our paraffin molds release easily? Thanks, Carol Torrence ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone marrow blocks
I sent out a request for information a while back and am going to ask again from a different approach. I am finding problems with embedding blocks of bone marrow particles with or without blood clot. Does anyone have a special technique they would like to share to concentrate the particles centrally in the block. Our current technique has the particles put in a block and then they are tamped down causing them to disperse throughout the block. Sometimes this results in the particles being located around the perimeter of the block with very little centrally positioned yielding extensive time to scan the entire slide because they are so spaced out. Do you let the sample clot or do you use anti-coagulant? Are the particles separated or are they mixed in with the blood? Any suggestions will be greatly appreciated Thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bone marrow aspirations
Can anyone share with me their process for processing bone marrow aspirations and embedding them in paraffin blocks. Currently ours are being spread out throughout the entire base mold and makes it cumbersome to screen. Has anyone used histogel or making a button and embed that instead of just filtering the aspirate and putting that is a cassette. Thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reagent containers for VIP5
Does anyone have spare reagent containers for a VIP5 they are willing to sell. I am looking for 3 to use exclusively for warm water wash. We are located in Ontario, Canada Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] standards for embedding
I know this topic has been beaten to death in the past and do realize it is dependent on tissue orientation, quantity of pieces in a block, and experience. There are guidelines suggested by the College of Med Lab of Ontario in a document Oct 2008 that indicates the expected minimum daily range is Specimens with no orientation 16-38 seconds per specimen Specimens with orientation21 to 52 seconds peer specimen Mixed cassettes 60-70 per hour I would like to know if this is monitored and what do you if your techs are unable to reach these recommendations, even after several years of experience. I realize these are minimum suggestions and should be achievable but what recommendations are suggested if a tech with over 10 years experience still averages close to 2 minutes a block. On average we may have 120-140 blocks a day and it takes 3.5-4 hours to embed. I have had ergonomic assessments done. They seem to maintain a constant level and will not strive for improvement. Any suggestions? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alcian Blue
What is the shelf life of prepared 1% Alcian blue in 3% Acetic Acid? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cresyl violet stain for HP
Is anyone willing to share their staining protocol for Cresyl Violet stain for HP Thanks Diana McCaig ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LEICA 6025 PROCESSOR
We are going to be setting up a Leica 6025 Processor. Can anyone send me their SOP and worksheets that you have created so I can use them as a reference and modify them according to our usage. Would truly be appreciated as well as any helpful tips or tricks you have encountered. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decal affecting IHC
Does decalcifying tissue (Calex II) affect the antibody reaction for IHC in bony tissue? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stain for HP
What stain are you using for HP?--Giemsa. Warthin Starry or IHC. Do you do them routinely or only when requested? Are they done on an autostainer or with a kit? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Breast processing times
Can you please give me your routine for processing breast cases over the weekend. Do you extend the time in alcohol to achieve the 48 hour maximum, call someone in over the weekend, or just indicate on the patient report that the fixation extended beyond the 48 hours limit for HER 2, 72 hours for ER and PR. Is the ischemic time recorded within the lab as well as on the patient report? Thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thickness of slides
Can you tell me what thickness you cut your routine slides for HE and immuno (in particular lymph nodes). Also, your protocol for cutting prostate needle core biopsies.how many spares you cut and do you designate the level on the slide label if multiple levels are submitted on one slide? Thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] air drying special stain slides rather than dehydrate and clear
I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to air dry (probably not set standard time) and dipped in xylene prior to cover slipping. Reason given is that the counterstain gets washed out. Wouldn't adjusting the times be a better resolution. I understand residual water could be present and cause long term issues on storage but wanted some other opinions on this process. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: air drying special stain slides rather than
Would this work for auto cover slipping (tape film)if they were set in the xylene reservoir prior to cover slipping? Diana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson Sent: September-11-12 1:15 PM To: Rene J Buesa Cc: 'histonet@lists.utsouthwestern.edu'; Mayer, Toysha N Subject: Re: [Histonet] RE: air drying special stain slides rather than I am convinced to give it a try because I also have trouble will the loss of some stains in dehydration. I was concerned that the slides would not clear well after oven dehydration. I will see how it works for me. I can see clearly how going from counterstain to oven will save much hassle with xylene and alcohols as well as not washing out some special stains. I have tried some of the isopropyl alcohol and acetone dehydration called for in some of the stain procedures and it would be great if the slides could just be popped into the oven. What mounting medium are you using? Does it matter? I am a bit worried about penetration of the mountant into the tissue section if there is no xylene in the tissue. Will neutral balsam still work ok? Rene: if you have a link to the paper you talked about on eliminating xylene, I am interested. Xylene is becoming more and more of an issue and a pain for us. EWJohnson Enruikang Ag Tech Beijing. On 9/12/2012 12:01 AM, Rene J Buesa wrote: Toysha: Perhaps you have not oven dried stained slides before, and that explains some of your comments, like: 1- if the stained slides are completely dried, the miscibility you point out is not an issues, because there is nothing to mix with; 2- if you dehydrate → clear the stained sections that will take about 15 minutes per group of up to 25 slides, or even more depending on the protocol used in your automated stainer, but if your group of slides in their rack are placed in an oven at 60ºC for 5 minutes it will just that, 5 minutes reducing the usual TAT for each staining procedure; 3- any oven can accommodate more than 100 stained slides in their racks and the TAT is shortened by oven drying, no matter how many slides you are working with; 4- I really do not know where you can find that extreme heat can affect the tissue sections. All tissue sections are fixed → processed → dried (usually at the same 60ºC before staining) → stained and an additional step at 60ºC to dry before cover-slipping is just that, an additional step at 60ºC 5- The so called Lean technologies do not refer to staining only, they have to do with the whole work-flow and an additional drying step at 60ºC cannot affect in a negative way to the work-flow 6- after staining you will oven dry the sections. I think you should try the method instead. René J. From: Mayer,Toysha Ntnma...@mdanderson.org To: 'histonet@lists.utsouthwestern.edu'histo...@lists.utsouthwestern.ed u Sent: Tuesday, September 11, 2012 11:41 AM Subject: [Histonet] RE: air drying special stain slides rather than Ooh, great question for my students next semester. Your answer is the counterstain, some counterstains may require dehydration after rinsing, or some may not. Adjusting the times of the counterstain is not the issue as much as the solvent of the counterstain. Rene, while I do acknowledge that the xylene may/will cause hazards, we must think of the miscibility of the clearant and the dehydrant, as well as the amount of time involved. The amount of time involved to blot and air dry the slides will affect the TAT for the specimen. 5 min may be ok if you have a small amount of slides, but with a larger number of slides, it will be considerably more than 5. Also Lean methodologies would not apply in that case. With automation, the extreme heat involved with a stain dryer may affect the tissue on the slide. There are some stains that can be blotted, cleared and coverslipped, but using the alcohol to remove excess water and counter stain is better in my opinion. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 16 Date: Tue, 11 Sep 2012 10:32:08 -0400 From: Diana McCaigdmcc...@ckha.on.ca Subject: [Histonet] air drying special stain slides rather than dehydrateand clear To:histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain;charset=us-ascii I was hoping to get information on why special stains are dehydrated, cleared and mounted vs allowing them to be blotted dry, air dried then coverslip. Every procedure I have ever encountered always indicates to dehydrate and clear but I have heard where some labs are blotting the slides , allowing to
[Histonet] counterstain for Alcian Blue (ph2.5)
Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] restaining negative stain with synaptophysin
We have no tissue in a block and would like to take the negative control and stain it for synaptophysin. We are using the Nemesis, Biocare Mach 4, AP protocol. Sincere thanks Diana McCaig ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Quality assurance program for pathologists
Is anyone willing to share with me their quality assurance/management program for pathologists. Sincere thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PowerPath Turn Around Time Report
Hi Does anyone using PowerPath have a turn around time report? Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DRYING TIMES for immuno stains
Can you tell me how long you let your IHC slides air dry at room temperature and how long you put them in the oven prior to staining? Thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biocare decloaker
We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SMM-HC
We are using Biocare Smooth Muscle Myosin-Heavy Chains (AP Protocol using Warp Red and previously Vulcan Fast Red)on the Nemesis auto stainer.. Lately we have notice considerable background staining. Any suggestions. The slides almost look like in the old days when you put too much albumin ( as an adhesive) on a slide and did an HE and the eosin coated the slide. We do not notice this with other antibodies. Any suggestions? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] uric acid crystals in tissue for gout
Does anyone have a method for identifying uric acid crystals in gout on a tissue sample. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MTS/Verhoeff's Stain
Does anyone have a procedure for a combination MTS/VG elastic stain that they would be willing to share? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] diastase
I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] negative controls on immunos
If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control sections for frozen sections
Does anyone run a HE control prior to staining a frozen section? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAS STAIN
Hi We have been doing a PAS stain on the same control block and same reagent supplier for a long time. Yesterday when we ran the slides, they failed to stain We opened new bottles of Periodic Acid and Schiff's Reagent and recut fresh controls Still, we are unable to get any staining. Suggestions to help would be appreciated Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] inking dyes
Can someone provide me with a supplier of tissue marking dyes for use on the grossing bench. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AB-PAS for Barrett's Esophagus
Can anyone share with me their procedure for AB-PAS for Barrett's Esophagus. With thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] processing schedule for needle core biopsies
We have been notices folds and wrinkles in the needle cores biopsies that do not seem to be affected by increased soaking. I am inclined to think it has to with processing. Currently they are done with the routine surgical specimens .What is the preferred schedule for needle cores? Is it best to excluded them from the main processor and do a separate run with reduced times? thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HPS Stain
I think there is confusion regarding the original question posed. They were not asking about the HPS stain. Some hospitals use an eosin/saffron stain in their routine HE. I had another lab recently asking about the formula to prepare this solution instead of eosin. Any advise? Diana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Wednesday, March 17, 2010 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HPS Stain Hi Sheila, HPS (Hematoxylin-Phloxine-Saffron) is the routine stain we use. There are a few institutions in Canada that also still use this stain; around Ottawa and a few other places in Ontario and Quebec. Perhaps one of these institutions sent slides to your pathologist for referral/consult? It is quite a nice trichrome stain, but somewhat more complex and expensive, uses more staining dishes, and it's sometimes tricky to get a proper phloxine/saffron balance. Due to the use of HE being more widespread, our pathology residents usually ask for HE slides for their files when preparing for exams. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] minimum fixation time for needle core prostate biopsies
Is there a minimum time for prostate biopsies to be fixed. Does fixation times affect PIN4's? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cassette labelers with bar codes
Can anyone advise me on cassette labelers that print 2D bar codes? Preferences or suggestions what to look for. Diana McCaig Histology Lab Chatham Kent Health Alliance 80 Grand Avenue West Chatham. Ontario N7L 1B7 519-352-6401 (6604) This email communication and any files transmitted with it may contain confidential and or proprietary information and is provided for the use of the intended recipient only. Any review, retransmission or dissemination of this information by anyone other than the intended recipient is prohibited. If you receive this email in error, please contact the sender and delete this communication and any copies immediately. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alcian Blue
In the past, when we made up 1% Alcian blue in 3% acetic acid (using bottle distilled water), the pH was always 2.5. We did not have to modify it to get the proper pH. Still using the same powder, we are getting a pH of 2.1. I hate to adjust it because it seems to affect the staining. Any suggestions. The pH meter is calibrated monthly. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IMMUNO STAINING--DECOLORIZING
We had a series of PIN 4 cocktail immuno stains and got a poor reaction. I am afraid to cut deeper in the block and miss the area of concern. I have determined the problem was due to the heat bar not being turned on. What can I do the slides to reverse the process and restain them. The DAB stained good, it is the vulcan fast red that was not reacting. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ER/PR scoring guidelines
Can someone provide me with the guidelines (Canadian) for scoring ER/PR and what the preferred antibody is for determining this. I see there are several clones. Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
What should the pH range be for Scott's Tap Water? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
