RE: [Histonet] Xylene Substitutes
Donna S. Ing= ersoll, B.S., HTL, CT(ASCP) Laboratory Manager A P Laboratories, LLC 2090 Executive Hall Rd S= uite 165 Charleston= , SC 29407 843-300-= 3001 X 202 843-300-= 3003 (fax) [1]dingers...@aplaboratories.com The contents of this message, together = with any attachments, are intended only for the use of the person(s) to whi= ch they are addressed and may contain confidential and/or privileged inform ation. Further, any medical information herein is confidential and protecte= d by law. It is unlawful for unauthorized persons to use, review, copy, dis= close, or disseminate confidential medical information. If you are not the = intended recipient, immediately advise the sender and delete this message a= nd any attachments. Any distribution, or copying of this message, or any at= tachment, is prohibited. Original M= essage Subject: Re: [Histonet] Xylene Substitutes From= : Rene J Buesa [2]rjbu...@yahoo.com Date: Wed, August 21, 2013 12:06 pm To:Yaskovich,Ruth=A(NIH/NIDCR)[E][3]rya skov...@dir.nidcr.nih.gov, '[4]Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwest= ern.edu Ruth: I all reality none is good. B= oth alkanes or D-Limonene are either as dangerous or more than xylene, and = all perform below the xylene standard. Any one requires modifications= of all procedures, they seldom dewax adequately and all constitute a proce= ssing compromise. Many cannot de recycled. Please go to [5]http://www.histosearch.com/rene.html= /a and read about xylene substitution. The optimal solu= tion to eliminate xylene consists on: 1- dehydrate with 2-propanol (o= r pure isopropyl alcohol or IPA) 2- do not use any ante-medium and = go from IPA directly to paraffin wax or ideally to mineral oil:IPA at = 1:1 3- infiltrate as usual 4- dewax sections with 2%aq. sol. of= dishwater soap at 90ºC for 1 min twice 5- stain as usual = 6- dehydrate stained sections in a convection oven at 60ºC for 5 minut= es 7- coverslip as usual. As you can see xylene is nowhere to b= e found in this sequence that produce optimal quality slides. Try it! René J. From: Yaskovich, Ruth A (NIH/NIDCR) [E] [6]ryaskov...@dir.nidcr.nih.gov To: '[7]Histonet@lists.utsouthwes= tern.edu' [8]His to...@lists.utsouthwestern.edu Sent: Wednesday, August 21, 2= 013 11:18 AM Subject: [Histonet] Xylene Substitutes = I know this question has been asked before but what's the best and safes= t Xylene Substitute? Ruth Yaskovich N.I.H. N.I.D.C.R. ___= Histonet mailing list [9]histo...@lists.uts= outhwestern.edu [10]http://lists.utsouthwestern.edu/mailman/listinfo/histo= net ___ Histone= t mailing list [11]H= isto...@lists.utsouthwestern.edu [12]http://lists.utsouthwestern.edu/mailm= an/listinfo/histonet References 1. =mailto:dingers...@aplaboratories.com; 2. 3Dmailto:rjbu...@yahoo.com; 3. 3Dmailto:ryaskov...@dir.nidcr.nih.gov; 4. 3Dmailto:Histonet@lists 5. file://localhost/tmp/3D 6. 3Dmailto:ryaskov 7. 3Dmailto:Histonet@lists.utsouthwestern.edu; 8. 3Dmailto:Histonet@lists.utsouthwestern.edu; 9. 3Dmailto:Histonet@lists.utsouthwestern.edu; 10. 3Dhttp://lists.utsouthwestern.edu/mailma 11. 3Dmailto:Histonet@lists.utsouthwestern.edu; 12. 3Dhttp://lists.utsouthw=/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HIER pressure cookers....
dingers...@aplaboratories.com Original Message Subject: RE: [Histonet] HIER = pressure cookers From: Blazek, Linda [1]lbla...@digestivespecialists.com = Date: Tue, April 30, 2013 9:58 am To: Connolly, Brett M brett_conno...@merck.com, = k. leigh adams [2]k.= leigh.adams@gmail.com, [3]histonet@lists.utsouthwestern.edu [4]histonet@lists.utsouthwestern.edu= /a I second that! Linda Blazek HT (ASCP) = Manager/Supervisor GI Pathology of Dayton Digestive Speciali= sts, Inc Phone: (937) 396-2623 Email: [5]lbla...@digestivespecialists.com = -Original Message- From:[6]histonet-bounces@lists.utsouthwes=tern.edu[[7] mailto:histonet-boun...@lists.utsouthwestern.edu]OnBehalf Of Connolly= , Brett M Sent: Tuesday, April 30, 2013 9:54 AM To: k. leigh ad= ams; [8]histo...@lists.ut= southwestern.edu Subject: RE: [Histonet] HIER pressure cookers...= . Biocare Decloaker here !! Brett M. Connolly, Ph= .D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 [9]brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -O= riginal Message- From: [10]histonet-boun...@lists.utsouthwestern.edu [[11]mailto:histonet-bounc= e...@lists.utsouthwestern.edu] On Behalf Of k. leigh adams Sent: Tu= esday, April 30, 2013 9:30 AM To: [12]histonet@lists.utsouthwestern.edu Subject: [His= tonet] HIER pressure cookers Any input as to preferred ins= truments would be greatly appreciated... Leigh _= __ Histonet mailing list [13]Histonet@lists.utsouthwe= stern.edu [14]http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contai ns information of MerckCo., Inc. (One Merck Drive, Whitehouse Statio= n, New Jersey, USA 08889), and/or its affiliates Direct contact information= for affiliates is available at [15]http://www.merck.com/contact/contacts.html) that may = be confidential, proprietary copyrighted and/or legally privileged. It is i= ntended solely for the use of the individual or entity named on this messag= e. If you are not the intended recipient, and have received this message in= error, please notify us immediately by reply e-mail and then delete it fro= m your system. = ___ Histonet mailing list [16]Histonet@lists.utsouthwestern.edu [17]http://lists.uts= outhwestern.edu/mailman/listinfo/histonet = ___ Histonet mailing list [18]Histonet@lists.utsouthwestern= .edu [19]http://lists.utsouthwestern.edu/mailman/listinfo/histonet = References 1. 3Dmailto:lbla 2. 3Dmailto:k.leigh.adams@gmail.com; 3. 3Dmailto:histonet@lists.utsout 4. file://localhost/tmp/3Dm 5. 3Dmailto:lblazek 6. 3Dmailto:h 7. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu; 8. 3Dmailto:histonet@lists.utsouthwestern.edu; 9. 3Dmailto:bre 10. 3Dmailto:histonet-bounces@lists.u 11. =mailto:histonet-boun...@lists.utsouthwestern.edu; 12. 3Dmailto:histo...@lists.ut 13. 3Dmailto:Histonet@lists.utsouthwestern.edu; 14. 3Dhttp://lists.utsouthwestern.edu/mailman/list 15. 3Dhttp://www.merck.com/conta 16. 3Dmailto:Histonet@lists. 17. file://localhost/tmp/3D 18. =mailto:Histonet@lists.utsouthwestern.edu; 19. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Histonet Digest, Vol 113, Issue 30
dingers...@aplaboratories.com ---= - Original Message Subject: [Histonet] RE: Histonet Dige= st, Vol 113, Issue 30 From: Barr,Kaye H [1]khb...@mdanderson.org Date: Tue, April 30, = 2013 1:48 pm To: '[2]histonet@lists.utsouthwestern.edu' [3]histonet@lists.utsouthwestern.edu What title do most use for someone who does gross and is not a P A? -Original Message- From:=[4]histonet-boun=c...@lists.utsouthwestern.edu [[5]mailto:histonet-boun...@lists.utsouthwestern.edu] O= n Behalf Of [6]hi= stonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, April 30, = 2013 12:02 PM To: [7]histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol= 113, Issue 30 Send Histonet mailing list submissions to = [8]histonet@lists.u= tsouthwestern.edu To subscribe or unsubscribe via the Worl= d Wide Web, visit [9]http://lists.utsouthwestern.edu/mailman/listinfo/hist= onet or, via email, send a message with subject or body 'help' to [10]hist= onet-requ...@lists.utsouthwestern.edu You can reach the pe= rson managing the list at [11]histonet-ow...@lists.utsouthwestern.edu = When replying, please edit your Subject line so it is more specific = than Re: Contents of Histonet digest... Today's To= pics: 1. BIG PROBLEM (Behnaz Sohrab) 2. Re: BIG PR= OBLEM (Linda) 3. Yearly Training (Nancy Schmitt) 4. HIER = pressure cookers (k. leigh adams) 5. RE: HIER pressure cookers= (Connolly, Brett M) 6. RE: HIER pressure cookers (Blazek,= Linda) 7. Re: HIER pressure cookers (Jan Shivers) 8.= RE: 5 gal specimen container search (Hannen, Valerie) 9. RE: 5 ga= l specimen container search (Shirley A. Powell) 10. RE: HIER pressu= re cookers ([12]Dingersoll @aplaboratories.com) 11. AW: [Histonet] BIG PROBLEM (Gudrun Lan= g) 12. Processor question - alcoholic formalin vs. buffered forma lin (Lake, Kim S) 13. RE: Processor question - alcoholi= c formalin vs. buffered formalin (Hannen, Valerie) 14. = cassette labelers (Margiotta-Watz, Michele) --- --- Me= ssage: 1 Date: Mon, 29 Apr 2013 10:36:01 -0700 From: Behnaz So= hrab [13]sohra...@ah.org = Subject: [Histonet] BIG PROBLEM To: [14]histonet@lists.utsouthwestern.edu Mes= sage-ID: [15]517E4D11.4347.0= 05...@ah.org Content-Type: text/plain; charset=us-ascii Good Morning, We have two Leica HE stainer( old xt-5010 an= d new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Ac= id 40 seconds Bluing : 45 sec. We done this for years!! last few mont= hs our Pantologists are complaining about GI Biopsies that they have TOO mu ch blue in cytoplasma and the Mucin is too purple and blue? Please any sugg= estion?? Mean while we are trying the NEW kit from Leica for HE = , they think that is TOO pale?any of you has tried this new stain from Leci a? With much application. Behnaz Sohrab, Los Angeles -- Message: 2 Dat= e: Mon, 29 Apr 2013 11:30:38 -0700 (PDT) From: Linda [16]lmd...@yahoo.com Subject: Re: [Histon= et] BIG PROBLEM To: Behnaz Sohrab [17]sohra...@ah.org, [18]histonet@lists.utsouthwestern.edu histonet@lists.utsouthwester= n.edu Message-ID: [19]1367260238.82176.YahooMailN e...@web164002.mail.gq1.yahoo.com Content-Type: text/plain; cha= rset=iso-8859-1 Hi, ? I would first check with y= our alcohol and xylene suppliers to see if they changed anything.? When som= ething?goes south it is usually because the manufacturer have changed.? Thi= s happened with my silver stains. ? You may have to go back to = the basics- harris hematoxylin, acid alcohol, and eosin y.? Richard Allen 7= 211 hematoxylin and the rest of their staining is excellent in these situat= ions until you can get it back under control.? If you send them slides they= will work a time out for you. ? If you are using Leica's selec= tech, which is what I started this lab out with because I was heavily sampl= ed out,?it has blue nuclei instead of the traditional purple.? I do 5 minut= es in hematoxylin, 30 dips in clarifier and 1 minute in blue, 55 sec in eos= in but I have (2) 95s after.? They also have a tech dept to help.? I have a Leica autostainer too. ? I hope this helps. ? Lind= a Dee, HT(ASCP) ? __= __ From: Behnaz Sohrab [20]Sohrab= b...@ah.org To: [21]histonet@lists.utsouthwestern.edu Sent: Monday, April 29, = 2013 12:36
[Histonet] Expired slides
dingersoll@aplabo= ratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] [FWD: Opportunity in Charleston, SC]
dingers...@aplaboratories.com
= ;
=
Original Message
Subject: = Opportunity in Charleston, SC
From: [1]dingers...@aplaboratories.com
Date: Tue, March = 05, 2013 4:20 pm
To: [2]histonet-requ...@lists.utsouthwestern.edu
AP Laboratories, LLC, a rapidly= growing anatomic pathology laboratory
in Charleston, SC, is accepting appl= ications for full and part time
histology technicians/technologists and gro= ssing technicians. We
offer an excellent compensation and benefit pac= kage to full time
employees. Pleaseemail your resume to [3]dingersoll@a
plaboratories.com or visit our website at
[4]www.aplaboratories.com = nbsp;
Donna S. Ingersoll, B.S., HTL, CT(ASCP)
=
Laboratory Manager
A P Laboratories LLC
8= 43-300-3001 x 202
[5]dingers...@aplaboratories.com
References
1. 3Dmailto:Dingersoll@apl 2. 3Dmailto:histonet-request@lists.utsouthwes
3. file://localhost/tmp/3Dm 4. 3Dhttp://www.aplabor=/
5.
3Dmailto:dingersoll@aplaboratories.___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] used lab equipment
I posted last week and got several responses. Anyone interested i= n obtaining this equipment, please email me what you are willing to pay= BRfor it. Whoever buys must also agree to pay for all packaging, handli= ng, freight and shipping costs related to the equipment. I will accept e= mails related to this equipment until Friday 11/9/12 We have the following equipment: Leica TP1050 (Tissue Processor) Dako Autostaine= r (48 slide capacity, unsure of software version) RMC Products MR3 Autom= ated microtome Fisher Scientific IsoTemp oven 2 Ventanna Benchmarks I just took over as lab manager a couple =f months ago and I'm not BRsure if all of the above are operational. I do kn=w that the Benchm= ark XTs do work and the Leica TP1050 does not work. Price is VERY negoti= able. Donna S. Ingersoll, B.S., HTL, CT(ASCP) Laboratory Manager= BRA P Laboratories LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Disposal of used lab equipment
We have upgraded some of our equipment in recent months and repla= ced some VERY OLD equipment that is just taking up space now. = I have contacted some of the used equipment man= ufacturers and no one is interested in taking this equipment off our hands. My question is how do you dispose = of used histology equipment. I don't know if our local landfill will = take it. Is there a service out there who will come haul it away? I appreciate any feedback. = ; Donna S. Ingersoll, B.S., HTL, CT(ASCP) n= bsp; ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Disposal of used lab equipment
We have the following equipment:
=
Leica TP1050 (Ti= ssue Processor)
Dako Autostainer (48 = slide capacity, unsure of software version)
RMC Products MR3 Automated microtome
Fisher Scientific IsoTemp oven
2 Ventanna Benchmark XT
I just took over as lab manager a couple = of months ago and I'm not
sure if all of the above are operational. I do kn= ow that the
Benchmark XTs do work and the Leica TP1050 does not work.
Price is VERY negotiable.
Donna S. Ing= ersoll, B.S., HTL, CT(ASCP)
Original Message
Subject: [Hi= stonet] Disposal of used lab equipment
From: [1]dingers...@aplaboratories.com
Date: Th= u, November 01, 2012 11:34 am
To: [2]histonet@lists.utsouthwestern.edu
We have upg= raded some of our equipment in recent months and repla=
ced
some VERY = OLD equipment that is just taking up space now.
=
I have contacted some of the used equipment man= ufacture= rs and no
one
is interested in taking this equipment off our hands.
= BR
My question is how do you dispose = of use= d histology equipment. I
don't know if our local landfill will = take = it. Is there a
service
out there who will come haul it away?
I appreciate any feedback.
= ;
Donna S. Ing= ersoll, B.S., HTL, CT(ASCP)
n= bsp;
__= _
Histonet mailing list
[3]Histonet@lists.utsouthwestern= .edu
[4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3Dmailto:Dinge 2. 3Dmailto:histonet@lists.utsou 3.
=mailto:Histonet@lists.utsouthwestern.edu;
4.
3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/his___
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